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The development of rapid chromatographic procedures is of utmost relevance to analytical laboratories since the modern pharmaceutical business is continually developing faster and more precise methods of drug discovery, development, distribution, and monitoring. Comprehensive two-dimensional (2D) chromatography is being developed as a tool to address challenges in the analysis of complex materials. Complex sample analyses may yield data that is challenging to quickly and accurately process. Here, chemometric analysis of data has shown to be the most fruitful and illuminating method. There are several applications for the determination of organic chemicals in samples with varying matrices. Gas/liquid chromatographic techniques are used for the bulk of organic chemical determinations. Therefore, it is crucial that these processes have minimal effects on the natural world. The collection and processing of samples, chromatographic separation, and final determination might all have less environmental impact if chromatographic methods were used. In both GC and LC, multidimensional separation methods may be used to improve the environmental friendliness of the analysis. The study describes 2D chromatographic techniques for GC-MS, 2D-LC, 2D-GC, 2D-CE, FTIR, and 2D-IR. Moreover, their advantages, challenges, and previous analytical data are also covered., Author(s): M. Sudheeshna [sup.1] , M. Malarvannan [sup.1] , K. Vinod Kumar [sup.1] , G. Kranthi Kumar [sup.1] , Y. Padmanabha Reddy [sup.1] Author Affiliations: (1) Department of Pharmaceutical Analysis, [...]

Globally, campylobacteriosis is one of the most common causes of gastroenteritis in humans. Campylobacter is an enteric pathogen present as a commensal in the gastrointestinal tract of a wide variety of animals and birds. Poultry, pig and livestock are the main reservoirs and the disease spread mainly by consumption of contaminated meat and milk. Contact with companion animals and contaminated environment (water and soil) also add to the risk of acquiring this infection. In the light of increasing trend of rearing pets in households in the post-covid period, the risk of Campylobacter infection from cats has to be investigated. Rats which are peri-domestic animals may contaminate the livestock farms rearing food animals and so may serve as a source of transmission of the disease. Rectal swabs were collected from cats (70) presented to veterinary hospitals and various households, and caecal samples were collected from rats (60) caught from different households and farm premises of Thrissur district. Campylobacter could not be detected in samples from cats by conventional culture technique and direct broth polymerase chain reaction (PCR). Out of the 60 samples collected from rats, no samples were positive for Campylobacter by conventional culture technique, but six (10 %) samples collected from rats procured from poultry farm premises were found to be positive for Campylobacter spp. by direct broth PCR. Of the six isolates obtained by direct PCR, five were identified as C. jejuni and one as C. coli. The presence of Campylobacter spp. in rat, increase the risk of transmission of the bacteria to farm animals and thereby potentially contaminate the food chain, resulting in human infections. A one health approach is needed to combat the occurrence and transmission of the disease in animals and humans. [ABSTRACT FROM AUTHOR]

Quality by Design (QbD) is an essential approach to pharmaceutical development and manufacturing that has garnered significant attention in recent years. Quality in services, products, and procedures equates to customer satisfaction. Consequently, it facilitates a transition in the pharmaceutical sector and the Food and Drug Administration (FDA) toward a more scientific, risk-based, comprehensive, and proactive drug development strategy. The pharmaceutical industry is actively seeking new solutions to ensure product quality and efficiency. This paper provides a comprehensive overview of QbD principles and their application in the pharmaceutical industry. The benefits of implementing QbD principles are discussed, encompassing increased efficiency, reduced costs, and improved product quality, safety, and efficacy. As the pharmaceutical industry continues to evolve, QbD will remain a crucial aspect of drug development and manufacturing. This article aims to provide pharmaceutical professionals with a comprehensive understanding of the QbD approach. [ABSTRACT FROM AUTHOR]