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495 results on '"Kumagai J"'

2. 058 A Rare Case of Spinal and Bulbar Muscular Atrophy (SMBA) Diagnosed by Hypertestosteronemia During Infertility Treatment

Author

Tai, T., primary, Igarashi, H., additional, Takesige, Y., additional, Nakamura, Y., additional, Hattori, H., additional, Nakajo, Y., additional, Aono, N., additional, Kasajima, M., additional, Yoshinaga, K., additional, Koizumi, M., additional, Hashimoto, T., additional, Toya, M., additional, Kumagai, J., additional, and Kyono, K., additional

Published
2022
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9. A bypass mechanism of abiraterone-resistant prostate cancer: Accumulating CYP17A1 substrates activate androgen receptor signaling

Author

Moll, J.M. (Jan), Kumagai, J., Royen, M.E. (Martin), Teubel, W.J. (Wilma), Soest, R.J. (Robert Jan) van, French, P.J. (Pim), Homma, Y. (Yukio), Jenster, G.W. (Guido), Wit, R. (Ronald) de, Weerden, W.M. (Wytske) van, Moll, J.M. (Jan), Kumagai, J., Royen, M.E. (Martin), Teubel, W.J. (Wilma), Soest, R.J. (Robert Jan) van, French, P.J. (Pim), Homma, Y. (Yukio), Jenster, G.W. (Guido), Wit, R. (Ronald) de, and Weerden, W.M. (Wytske) van

Abstract

Background: Intratumoral steroidogenesis and its potential relevance in castration‐ resistant prostate cancer (CRPC) and in cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1)‐inhibitor treated hormone‐naïve and patients with CRPC are not well established. In this study, we tested if substrates for de novo steroidogenesis accumulating during CYP17A1 inhibition may drive cell growth in relevant preclinical models. Methods: PCa cell lines and their respective CRPC sublines were used to model CRPC in vitro. Precursor steroids pregnenolone (Preg) and progesterone (Prog) served as substrate for de novo steroid synthesis. TAK700 (orteronel), abiraterone, and small interfering RNA (siRNA) against CYP17A1 were used to block CYP17A1 enzyme activity. The antiandrogen RD162 was used to assess androgen receptor (AR) involvement. Cell growth was measured by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay. AR‐ target gene expression was quantified by reverse transcription polymerase chain reaction (RT‐PCR). Nuclear import studies using cells with green fluorescent protein (GFP)‐tagged AR were performed to assess the potential of precursor steroids to directly activate AR. Results: Preg and Prog stimulated cell proliferation and AR target gene expression in VCaP, DuCaP, LNCaP, and their respective CRPC sublines. The antiandrogen RD162, but not CYP17A1 inhibition with TAK700, abiraterone or siRNA, was able to block Preg‐ and Prog‐induced proliferation. In contrast to TAK700, abiraterone also affected dihydrotestosterone‐induced cell growth, indicating direct AR binding. Furthermore, Prog‐induced AR translocation was not affected by treatment with TAK700 or abiraterone, while it was effectively blocked by the AR antagonist enzalutamide, further demonstrating the direct AR activation by Prog. Conclusion: Activation of the AR by clinically relevant levels of Preg and Prog accumulating in abiraterone‐treated patients may act as a driver for CRPC. These d

Published
2019
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14. P838 Complicated disease in East Asian patients with ulcerative colitis over two decades: A single-centre retrospective cohort in Japan

Author

Taida, T, primary, Kumagai, J, additional, Ogasawara, S, additional, Ohta, Y, additional, Hamanaka, S, additional, Nakamura, M, additional, Ohyama, H, additional, Saito, K, additional, Kusakabe, Y, additional, Yasui, S, additional, Sugiyama, H, additional, Sakai, Y, additional, Mikata, R, additional, Nakagawa, T, additional, Katsuno, T, additional, Tsuyuguchi, T, additional, and Kato, N, additional

Published
2018
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19. 109 Expression of androgen and estrogen signalling components and stem cell markers is highly predictive cancer progression of metastatic prostate cancer

Author

Fujimura, T., primary, Takahashi, S., additional, Urano, T., additional, Takayama, K., additional, Sugihara, T., additional, Obinata, D., additional, Yamada, Y., additional, Kumagai, J., additional, Kume, H., additional, Ouchi, Y., additional, Inoue, S., additional, and Homma, Y., additional

Published
2014
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24. Increased expression of estrogen-related receptor alpha (ERRalpha) is a negative prognostic predictor in human prostate cancer.

Author

Fujimura, T., Takahashi, S., Urano, T., Kumagai, J., Ogushi, T., Horie-Inoue, K., Ouchi, Y., Kitamura, T., Muramatsu, M., Inoue, S., Fujimura, T., Takahashi, S., Urano, T., Kumagai, J., Ogushi, T., Horie-Inoue, K., Ouchi, Y., Kitamura, T., Muramatsu, M., and Inoue, S.

Abstract

Item does not contain fulltext, The nuclear receptor ERRalpha (estrogen-related receptor alpha) is known to modulate the estrogen-signaling pathway, but the biological significance of ERRalpha in the prostate remains unclear. We investigated the expression of ERRalpha in human prostate tissues and cancer cell lines to evaluate the potential roles of the receptor in prostate cancer (PC). Western blot analysis of ERRalpha was performed in three cell lines of human PC (LNCaP, DU145 and PC-3). The expressions of ERRalpha in cancerous lesions (n = 106) and benign foci (n = 99) of 106 surgically obtained prostate specimens were evaluated by immunohistochemistry. The relationships between the ERRalpha expression and clinicopathological features were evaluated. Western blot analysis using the polyclonal anti-ERRalpha antibody detected a 52 kD band in all three PC cell lines. Positive immunostaining of ERRalpha in the nuclei was found in 73 (69%) cancerous and 47 (47.5%) benign epithelium, whereas the stromal tissues were negative for ERRalpha. The mean immunoreactivity score (IR score) of the cancerous lesions (3.5 +/- 2.6) was significantly higher than that of the benign foci (1.8 +/- 2.1) (p < 0.0001). The IR score of the cancerous lesions significantly correlated with the Gleason score (p = 0.0135). Univariate and multivariate hazard analyses revealed significant correlations between elevated ERRalpha expression and poor cancer-specific survival (p = 0.0141 and 0.0367, respectively). The enhanced expression of ERRalpha might play a role in the development of human PC and serve as a significant prognostic factor for the disease.

Published
2007

25. Cytochrome P450 2B6 is a growth-inhibitory and prognostic factor for prostate cancer.

Author

Kumagai, J., Fujimura, T., Takahashi, S., Urano, T., Ogushi, T., Horie-Inoue, K., Ouchi, Y., Kitamura, T., Muramatsu, M., Blumberg, B., Inoue, S., Kumagai, J., Fujimura, T., Takahashi, S., Urano, T., Ogushi, T., Horie-Inoue, K., Ouchi, Y., Kitamura, T., Muramatsu, M., Blumberg, B., and Inoue, S.

Abstract

Item does not contain fulltext, BACKGROUND: Cytochrome P450s (CYPs) influence the biological effects of carcinogens, drugs and hormones including testosterones. Among them, Cytochrome P450 2B6 (CYP2B6) plays a critical role in the deactivation of testosterone. In the present study, we examined CYP2B6 expression in human prostate tissues and prostate cancer. METHODS: Immunohistochemical analysis was performed in 98 benign and 106 malignant prostate tissues and patients' charts were reviewed for clinical, pathologic and survival data. We also investigated whether stable expression of CYP2B6 in LNCaP (human prostate cancer cell line) influences cellular proliferation. RESULTS: CYP2B6 was abundantly expressed in the normal epithelial cells compared to the prostate cancer cells. Significant immunostaining of CYP2B6 was found in 75 of 106 samples (71%), in the cytoplasm of cancerous tissue samples. CYP2B6 immunoreactivity was inversely correlated with high Gleason score (P < 0.001). Decreased immunoreactivity of CYP2B6 significantly correlated with poor prognosis (P < 0.0001). Univariate and multivariate hazard analyses revealed a significant correlation of decreased CYP2B6 expression with poor cancer-specific survival (P = 0.0028 and 0.0142, respectively). Furthermore, overexpression of CYP2B6 in LNCaP cells significantly decreased testosterone-induced proliferation. CONCLUSIONS: These results demonstrated that decreased expression of CYP2B6 might play a role in the development of prostate cancer, and be useful as the prognostic predictor for human prostate cancer.

Published
2007

30. Immunohistochemical distribution of type I, II and III collagens in the rabbit supraspinatus tendon insertion

Author

Kumagai, J, Sarkar, K, Uhthoff, H K, Okawara, Y, and Ooshima, A

Subjects
Tendons, Cartilage, Animals, Female, Collagen, Rabbits, Humerus, musculoskeletal system, Immunohistochemistry, Research Article
Abstract

The collagen fibres in the area of attachment of the supraspinatus tendon to bone were studied immunohistochemically in 12 mature female New Zealand white rabbits. The labelling of type I collagen was uniformly prominent in the bone as well as in the fascicles of the tendon proper but inconspicuously scattered in the unmineralised and mineralised zones of the fibrocartilage. Type II collagen, not detected in the tendon proper, was widespread in both zones of the fibrocartilage. Type III collagen, on the other hand, appeared to be confined mainly to the zone of unmineralised fibrocartilage, in addition to its presence in the endotenon of the tendon proper. The region of the tidemark failed to show immunostaining for any of the collagen fibre types. In conclusion, this study demonstrates that, although all the principal fibrous collagen types are constituents of the supraspinatus tendon at its attachment site, the distribution pattern of immunolabelling varies from zone to zone.

Published
1994

31. Stem Cells

Author

He, H., primary, Emmett, M. R., additional, Marshall, A. G., additional, Ji, Y., additional, Conrad, C. A., additional, Priebe, W., additional, Colman, H., additional, Lang, F. F., additional, Madden, T. L., additional, Kristoffersen, K., additional, Stockhausen, M.-T., additional, Poulsen, H. S., additional, Binder, Z. A., additional, Orr, B., additional, Lim, M., additional, Weingart, J. D., additional, Brem, H., additional, Olivi, A., additional, Riggins, G. J., additional, Gallia, G. L., additional, Litofsky, N. S., additional, Miller, D. C., additional, Rath, P., additional, Anthony, D. C., additional, Feng, Q., additional, Franklin, C., additional, Pei, L., additional, Free, A., additional, Kirk, M. D., additional, Shi, H., additional, Timmer, M., additional, Theiss, H., additional, Juerchott, K., additional, Ries, C., additional, Paron, I., additional, Franz, W., additional, Selbig, J., additional, Guo, K., additional, Tonn, J. C., additional, Schichor, C., additional, Zhou, Y.-H., additional, Hu, Y., additional, Pioli, P. D., additional, Rajneesh, K., additional, Limoli, C. L., additional, Yu, L., additional, Hess, K. R., additional, Linskey, M. E., additional, Faber, F., additional, Jaeger, D., additional, Thorsteinsdottir, J., additional, Albrecht, V., additional, Tonn, J.-C., additional, Price, R., additional, Song, J., additional, Zimmerman, P., additional, Duale, H., additional, Rivera, A., additional, Kaur, B., additional, Parada, L., additional, Cook, C., additional, Chiocca, E. A., additional, Kwon, C.-H., additional, Munoz, D. M., additional, Guha, A., additional, Estrada-Bernal, A., additional, Van Brocklyn, J. R., additional, Gu, C., additional, Mahasenan, K. V., additional, Joshi, K., additional, Gupta, S., additional, Mattson, A., additional, Li, C., additional, Nakano, I., additional, Chi, A. S., additional, Rheinbay, E., additional, Wakimoto, H., additional, Gillespie, S., additional, Kasif, S., additional, Rabkin, S. D., additional, Martuza, R. L., additional, Bernstein, B. E., additional, Skirboll, S. L., additional, Wurdak, H., additional, Zhu, S., additional, Romero, A., additional, Lorger, M., additional, Watson, J., additional, Chiang, C.-y., additional, Zhang, J., additional, Natu, V. S., additional, Lairson, L. L., additional, Walker, J. R., additional, Trussell, C. M., additional, Harsh, G. R., additional, Vogel, H., additional, Felding-Habermann, B., additional, Orth, A. P., additional, Miraglia, L. J., additional, Rines, D. R., additional, Schultz, P. G., additional, Hide, T., additional, Takezaki, T., additional, Nakamura, H., additional, Makino, K., additional, Kuratsu, J.-i., additional, Kondo, T., additional, Yao, J., additional, Kim, Y.-W., additional, Koul, D., additional, Almeida, J. S., additional, Weinstein, J. N., additional, Alfred Yung, W. K., additional, Miyazaki, T., additional, Chaudhury, A. R., additional, Wong, A. J., additional, Del Vecchio, C., additional, Mitra, S., additional, Han, S.-Y., additional, Holgado-Madruga, M., additional, Gupta, P., additional, Golebiewska, A., additional, Brons, N. H., additional, Bjerkvig, R., additional, Niclou, S. P., additional, Ramm, P., additional, Vollmann-Zwerenz, A., additional, Beier, C., additional, Aigner, L., additional, Bogdahn, U., additional, Kalbitzer, H. R., additional, Hau, P., additional, Sanzey, M., additional, Vallar, L., additional, Tamura, K., additional, Aoyagi, M., additional, Ando, N., additional, Ogishima, T., additional, Yamamoto, M., additional, Ohno, K., additional, Perin, A., additional, Fung, K. H., additional, Longatti, P., additional, Guiot, M.-C., additional, Del Maestro, R. F., additional, Rossi, S., additional, Stechishin, O., additional, Weiss, S., additional, Stifani, S., additional, Goodman, L., additional, Gao, F., additional, Gumin, J., additional, Ezhilarasan, R., additional, Love, P., additional, George, A., additional, Lang, F., additional, Aldape, K., additional, Sulman, E. P., additional, Soeda, A., additional, Lee, D.-H., additional, Shaffrey, M. E., additional, Oldfield, E. H., additional, Park, D. M., additional, Dietrich, J., additional, Han, R., additional, Noble, M., additional, Yang, M. Y., additional, Liu, X., additional, Madhankumar, A. B., additional, Sheehan, J., additional, Slagle-Webb, B., additional, Connor, J. R., additional, Fu, J., additional, Shen, R.-J., additional, Kaluzova, M., additional, Machaidze, R., additional, Nduom, E. N. K., additional, Burden, C. T., additional, Hadjipanayis, C. G., additional, Lei, L., additional, Sonabend, A., additional, Guarnieri, P., additional, Ludwig, T., additional, Rosenfeld, S., additional, Bruce, J., additional, Canoll, P., additional, Vaillant, B. D., additional, Bhat, K., additional, Balasubramaniyam, V., additional, Wang, S., additional, Sulman, E., additional, Goodman, L. D., additional, Love, P. N., additional, Soules, M., additional, Zhu, T., additional, Flack, C., additional, Talsma, C., additional, Hamm, L., additional, Muraszko, K., additional, Fan, X., additional, Matsuoka, Y., additional, Kawano, Y., additional, Kobayashi, D., additional, Kumagai, J., additional, Frank, R. T., additional, Najbauer, J., additional, Aboody, K. S., additional, Metz, M., additional, Garcia, E., additional, Aramburo, S., additional, Valenzuela, V., additional, Gutova, M., additional, Annala, A. J., additional, Barish, M., additional, Danks, M., additional, Kim, S. U., additional, Portnow, J., additional, Hofstetter, C., additional, Gursel, D., additional, Mubita, L., additional, Holland, E., additional, Boockvar, J., additional, Monje, M., additional, Freret, M., additional, Masek, M., additional, Edwards, M. S., additional, Fisher, P. G., additional, and Beachy, P., additional

Published
2010
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33. Independent histological risk factors for lymph node metastasis of superficial esophageal squamous cell carcinoma; implication of claudin-5 immunohistochemistry for expanding the indications of endoscopic resection

Author

Chiba, T., primary, Kawachi, H., additional, Kawano, T., additional, Kumagai, J., additional, Kitagaki, K., additional, Sekine, M., additional, Uchida, K., additional, Kobayashi, M., additional, Sugihara, K., additional, and Eishi, Y., additional

Published
2009
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35. ESR, ENDOR, and ESEEM spectroscopy study on the local structure and motion of reactants : Highly selective tunneling radical abstraction in a neopentane-ethane mixture

Author

Kumagai, J, Itagaki, Y, Ishizuka, M, Kumada, T, Lund, Anders, Miyazaki, T, Kumagai, J, Itagaki, Y, Ishizuka, M, Kumada, T, Lund, Anders, and Miyazaki, T

Abstract

Local structures and motions of radical reactants in highly selective tunneling abstraction reaction in neopentane-ethane mixture are investigated with CW-ESR, ENDOR, and ESEEM spectroscopy at different temperatures. H atoms produced by the radiolysis of neo-C5H12 in the neo-C5H12-C2H6 (2 mol %) mixtures above 40 K react selectively with the solute C2H6 to give ethyl radicals. The selective reaction is suppressed below 30 K. The local structures of neopentane matrix around neopentyl and ethyl radicals are approximately the same in the temperature range from 4.4 to 45 K. Although neopentyl radicals are rigidly trapped below 100 K, ethyl radicals in neopentane matrix begin to librate even at 6 K and increase the degree of motion up to 30 K, Finally, the librating motion of the ethyl radicals in neopentane matrix is vigorous above 40 K. The ethane molecule can take more favorable orientation for the hydrogen abstraction reaction by H atoms than rigid neopentane molecules by the vigorous libration motion, resulting in the selective reaction with H atoms.

Published
2001
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