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668 results on '"Kulkarni, Ashok B."'

5. Meeting report: a hard look at the state of enamel research.

Author

Klein, Ophir D, Duverger, Olivier, Shaw, Wendy, Lacruz, Rodrigo S, Joester, Derk, Moradian-Oldak, Janet, Pugach, Megan K, Wright, J Timothy, Millar, Sarah E, Kulkarni, Ashok B, Bartlett, John D, Diekwisch, Thomas Gh, DenBesten, Pamela, and Simmer, James P

Subjects
Cell Line, Stem Cells, Dental Enamel, Animals, Humans, Cell Culture Techniques, Amelogenesis, enamel, mineralized tissue, mineralization, ameloblast, stem cell, Medical Biotechnology, Dentistry
Abstract

The Encouraging Novel Amelogenesis Models and Ex vivo cell Lines (ENAMEL) Development workshop was held on 23 June 2017 at the Bethesda headquarters of the National Institute of Dental and Craniofacial Research (NIDCR). Discussion topics included model organisms, stem cells/cell lines, and tissues/3D cell culture/organoids. Scientists from a number of disciplines, representing institutions from across the United States, gathered to discuss advances in our understanding of enamel, as well as future directions for the field.

Published
2017

8. PD-1 blockade attenuates immunosuppressive myeloid cells due to inhibition of CD47/SIRPα axis in HPV negative head and neck squamous cell carcinoma

Author

Yu, Guang-Tao, Bu, Lin-Lin, Huang, Cong-Fa, Zhang, Wen-Feng, Chen, Wan-Jun, Gutkind, J Silvio, Kulkarni, Ashok B, and Sun, Zhi-Jun

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Immunology, Dental/Oral and Craniofacial Disease, Clinical Research, Cancer, Rare Diseases, Aetiology, 2.1 Biological and endogenous factors, Animals, Antibodies, Monoclonal, Antigens, Differentiation, B7-H1 Antigen, Blotting, Western, CD47 Antigen, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Head and Neck Neoplasms, Humans, Immunohistochemistry, Macrophages, Mice, Knockout, Myeloid Cells, PTEN Phosphohydrolase, Papillomaviridae, Programmed Cell Death 1 Receptor, Protein Serine-Threonine Kinases, RNA Interference, Receptor, Transforming Growth Factor-beta Type I, Receptors, Immunologic, Receptors, Transforming Growth Factor beta, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, HNSCC, myeloid-derived suppressor cell, tumor associated macrophagy, PD-1, Protein-Serine-Threonine Kinases, Antigens, CD47, Antigens, CD274, Oncology and carcinogenesis
Abstract

Myeloid-derived suppressor cells (MDSCs) and tumor associated macrophages (TAMs) play key roles in the tumor immune suppressive network and tumor progression. However, precise roles of programmed death-1 (PD-1) in immunological functions of MDSCs and TAMs in head and neck squamous cell carcinoma (HNSCC) have not been clearly elucidated. In the present study, we show that PD-1 and PD-L1 levels were significantly higher in human HNSCC specimen than in normal oral mucosa. MDSCs and TAMs were characterized in mice and human HNSCC specimen, correlated well with PD-1 and PD-L1 expression. αPD-1 treatment was well tolerated and significantly reduced tumor growth in the HNSCC mouse model along with significant reduction in MDSCs and TAMs in immune organs and tumors. Molecular analysis suggests a reduction in the CD47/SIRPα pathway by PD-1 blockade, which regulates MDSCs, TAMs, dendritic cell as well as effector T cells. Hence, these data identify that PD-1/PD-L1 axis is significantly increased in human and mouse HNSCC. Adoptive αPD-1 immunotherapy may provide a novel therapeutic approach to modulate the micro- and macro-environment in HNSCC.

Published
2015

11. Photoactivation of Endogenous Latent Transforming Growth Factor–β1 Directs Dental Stem Cell Differentiation for Regeneration

Author

Arany, Praveen R, Cho, Andrew, Hunt, Tristan D, Sidhu, Gursimran, Shin, Kyungsup, Hahm, Eason, Huang, George X, Weaver, James, Chen, Aaron Chih-Hao, Padwa, Bonnie L, Hamblin, Michael R, Barcellos-Hoff, Mary Helen, Kulkarni, Ashok B, and J Mooney, David

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Dentistry, Dental/Oral and Craniofacial Disease, Transplantation, Stem Cell Research, Stem Cell Research - Nonembryonic - Non-Human, Regenerative Medicine, Development of treatments and therapeutic interventions, 5.2 Cellular and gene therapies, Animals, Cell Differentiation, Dentin, Mice, Stem Cells, Tooth, Transforming Growth Factor beta1, Biological Sciences, Medical and Health Sciences, Medical biotechnology, Biomedical engineering
Abstract

Rapid advancements in the field of stem cell biology have led to many current efforts to exploit stem cells as therapeutic agents in regenerative medicine. However, current ex vivo cell manipulations common to most regenerative approaches create a variety of technical and regulatory hurdles to their clinical translation, and even simpler approaches that use exogenous factors to differentiate tissue-resident stem cells carry significant off-target side effects. We show that non-ionizing, low-power laser (LPL) treatment can instead be used as a minimally invasive tool to activate an endogenous latent growth factor complex, transforming growth factor-β1 (TGF-β1), that subsequently differentiates host stem cells to promote tissue regeneration. LPL treatment induced reactive oxygen species (ROS) in a dose-dependent manner, which, in turn, activated latent TGF-β1 (LTGF-β1) via a specific methionine residue (at position 253 on LAP). Laser-activated TGF-β1 was capable of differentiating human dental stem cells in vitro. Further, an in vivo pulp capping model in rat teeth demonstrated significant increase in dentin regeneration after LPL treatment. These in vivo effects were abrogated in TGF-β receptor II (TGF-βRII) conditional knockout (DSPP(Cre)TGF-βRII(fl/fl)) mice or when wild-type mice were given a TGF-βRI inhibitor. These findings indicate a pivotal role for TGF-β in mediating LPL-induced dental tissue regeneration. More broadly, this work outlines a mechanistic basis for harnessing resident stem cells with a light-activated endogenous cue for clinical regenerative applications.

Published
2014

12. Behavioral and synaptic alterations relevant to obsessive-compulsive disorder in mice with increased EAAT3 expression

Author

Delgado-Acevedo, Claudia, Estay, Sebastián F., Radke, Anna K., Sengupta, Ayesha, Escobar, Angélica P., Henríquez-Belmar, Francisca, Reyes, Cristopher A., Haro-Acuña, Valentina, Utreras, Elías, Sotomayor-Zárate, Ramón, Cho, Andrew, Wendland, Jens R., Kulkarni, Ashok B., Holmes, Andrew, Murphy, Dennis L., Chávez, Andrés E., and Moya, Pablo R.

Published
2019
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25. ACTIVATION OF CYCLIN-DEPENDENT KINASE 5 BROADENS ACTION POTENTIALS IN HUMAN SENSORY NEURONS

Author

Tiwari, Manindra Nath, Hall, Bradford E., Terse, Anita, Amin, Niranjana, Chung, Man-Kyo, and Kulkarni, Ashok B.

Subjects
Article
Abstract

Chronic pain is one of the most devastating and unpleasant conditions, associated with many pathological conditions. Tissue or nerve injuries induce comprehensive neurobiological plasticity in nociceptive neurons, which leads to chronic pain. Recent studies suggest that cyclin-dependent kinase 5 (CDK5) in primary afferents is a key neuronal kinase that modulates nociception through phosphorylation-dependent manner under pathological conditions. However, the impact of the CDK5 on nociceptor activity especially in human sensory neurons are not known. To determine the CDK5-mediated regulation of human dorsal root ganglia (hDRG) neuronal properties, we have performed the whole-cell patch clamp recordings in neurons dissociated from hDRG. CDK5 activation induced by overexpression of p35 depolarized the resting membrane potential and reduced the rheobase currents as compared to the uninfected neurons. CDK5 activation evidently changed the shape of the action potential (AP) by increasing AP rise time, AP fall time, and AP half width. The application of a prostaglandin E2 (PG) and bradykinin (BK) cocktail in uninfected hDRG neurons induced the depolarization of RMP and the reduction of rheobase currents along with increased AP rise time. However, PG and BK applications failed to induce any further significant changes in addition to the aforementioned changes of the membrane properties and AP parameters in the p35-overexpressing group. We conclude that CDK5 activation through the overexpression of p35 in dissociated hDRG neurons broadens AP in hDRG neurons and that CDK5 may play important roles in the modulation of AP properties in human primary afferents under pathological conditions, contributing to chronic pain.

Published
2023

28. CCR Translation for This Article from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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29. Supplementary Figure 6 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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30. Supplementary Figure 4 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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31. Supplementary Figure 2 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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33. Supplementary Methods from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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35. Supplementary Figure 5 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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36. Supplementary Figure 8 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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37. Supplementary Table 2 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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38. Supplementary Figure Legend from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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39. Supplementary Table 3 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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40. Supplementary Figure 1 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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41. Supplementary Figure 3 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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42. Supplementary Figure 7 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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43. Supplementary Table 1 from PI3K/mTOR Inhibitor PF-04691502 Antitumor Activity Is Enhanced with Induction of Wild-Type TP53 in Human Xenograft and Murine Knockout Models of Head and Neck Cancer

Author

Herzog, Amanda, primary, Bian, Yansong, primary, Vander Broek, Robert, primary, Hall, Bradford, primary, Coupar, Jamie, primary, Cheng, Hui, primary, Sowers, Anastasia L., primary, Cook, John D., primary, Mitchell, James B., primary, Chen, Zhong, primary, Kulkarni, Ashok B., primary, and Van Waes, Carter, primary

Published
2023
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44. Data from Progressive Tumor Formation in Mice with Conditional Deletion of TGF-β Signaling in Head and Neck Epithelia Is Associated with Activation of the PI3K/Akt Pathway

Author

Bian, Yansong, primary, Terse, Anita, primary, Du, Juan, primary, Hall, Bradford, primary, Molinolo, Alfredo, primary, Zhang, Pin, primary, Chen, Wanjun, primary, Flanders, Kathleen C., primary, Gutkind, J. Silvio, primary, Wakefield, Lalage M., primary, and Kulkarni, Ashok B., primary

Published
2023
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45. Supplementary Figure 2 from Progressive Tumor Formation in Mice with Conditional Deletion of TGF-β Signaling in Head and Neck Epithelia Is Associated with Activation of the PI3K/Akt Pathway

Author

Bian, Yansong, primary, Terse, Anita, primary, Du, Juan, primary, Hall, Bradford, primary, Molinolo, Alfredo, primary, Zhang, Pin, primary, Chen, Wanjun, primary, Flanders, Kathleen C., primary, Gutkind, J. Silvio, primary, Wakefield, Lalage M., primary, and Kulkarni, Ashok B., primary

Published
2023
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46. Supplementary Figure 1 from Progressive Tumor Formation in Mice with Conditional Deletion of TGF-β Signaling in Head and Neck Epithelia Is Associated with Activation of the PI3K/Akt Pathway

Author

Bian, Yansong, primary, Terse, Anita, primary, Du, Juan, primary, Hall, Bradford, primary, Molinolo, Alfredo, primary, Zhang, Pin, primary, Chen, Wanjun, primary, Flanders, Kathleen C., primary, Gutkind, J. Silvio, primary, Wakefield, Lalage M., primary, and Kulkarni, Ashok B., primary

Published
2023
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47. Supplementary Methods, Figures 1-2 from Progressive Tumor Formation in Mice with Conditional Deletion of TGF-β Signaling in Head and Neck Epithelia Is Associated with Activation of the PI3K/Akt Pathway

Author

Bian, Yansong, primary, Terse, Anita, primary, Du, Juan, primary, Hall, Bradford, primary, Molinolo, Alfredo, primary, Zhang, Pin, primary, Chen, Wanjun, primary, Flanders, Kathleen C., primary, Gutkind, J. Silvio, primary, Wakefield, Lalage M., primary, and Kulkarni, Ashok B., primary

Published
2023
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