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The paper presents the results of the analysis of the usefulness of the mineral composition of diets and the effectiveness of the use of mineral and vitamin supplements in feeding cows of different technological groups. The analysis showed that the amount of calcium and phosphorus in the diets was close to the norm. Extremely high concentrations of elements such as magnesium, potassium and sodium were noted with an obvious lack of chlorine and sulfur. The cation-anion balance was positive: the range of indicators ranged from 14.98 to 23.49 and a tendency to increase this indicator by the end of lactation was revealed. The ratio of calcium and phosphorus, as well as potassium and sodium did not exceed the established norms. For most of the trace elements, the excess of the recommended norms was determined, which can be justified due to the low digestibility of trace elements in the body of cows.

The article presents the results of experimental studies on the use of nanosized iron powder in the third trimester of gestation on the reproduction processes of sows. The studies were carried out in the conditions of the farm. The physiological state of pregnant sows was determined by hematological parameters. It was found that iron NRP contributed to an increase in the number of red blood cells and hemoglobin in the blood of sows, compared with the control 20 days before farrowing, the content of red blood cells in the blood of experimental sows was 6.3% significantly higher compared to the control, and hemoglobin by 3.8 %. For 10 days, before farrowing, respectively, by 6.9 % and 5.2% higher. The indicators that characterize the reproductive ability of sows are: multiplicity, large fertility, the number of piglets in the nest and their average live weight. The use of ultrafine iron powder in the diets of pregnant sows in the third trimester of gestation contributed to the improvement of the overall physiological state, confirmed by the improvement of hematological parameters and had a positive effect on reproductive ability. The weight of the nest in the experimental group of sows on the day of farrowing was 4.1 % higher, the large-fruiting rate was 3.1 %, compared to the control animals.

Evaluates the acrosome status in human spermatozoa from normozoospermic men. Use of flow cytometry following the induction of the acrosome reaction with the ionophore A23187; Dual fluorescence staining of methanol fixed spermatozoa incubated with and without the ionophore A23187; Intensity of green and red fluorescence in acrosome-reacted spermatozoa.

The search for modern effective means for prevention and treatment of diseases of hooves of farm animals is an urgent and timely issue. The study considers assessment of the incidence and nature of hoof pathologies in highly productive cows in one of the livestock farms of Ryazan region with a production line system and loose housing of animals. The total percentage of lameness in the farm was 17.3%, including acute lameness, when the cow does not lean on a bad limb - 3.6%. After assessing the significance and relevance of the study for this farm, there was an experiment to assess the efficiency of the use of a new antiseptic drug for preventive and therapeutic treatment of hooves based on quaternary ammonium salts, nonionic surface-active materials, copper aldehyde and sulfate. The drug showed high bactericidal activity when studying the total microbial count (51.2% lower than in the control group) and the presence of E. coli bacteria (not detected after exposure). When assessing the presence and nature of the growth of microorganisms on the Endo nutrient medium in the experimental group of animals, the results similar to the comparison drug were noticed.

To study peculiarities of mental disorders in women during treatment of sterility using current reproductive technologies (extracorporeal fertilization), 512 women, mean age 33,6+3,7 years and sterility duration 3,1+/-2,2 years (from 1 to 5 years) have been examined. A number of attempts to treat sterility varied from 1 to 10. In 266 (52%) patients, borderline mental disorders have been found. Anxiety and depressive disorders made up 31,2%. A significant role of stress in their manifestation and a negative influence of mental disorders on treatment outcome and possibility of remission has been revealed. The use of cognitive-behavioral psychotherapy resulted in the decrease of expression of mental disorders and adequately prepared patients to the treatment thus improving its results.

By studying 211 females by currently available radiation techniques, such as X-ray study, ultrasonography, and magnetic resonance imaging mammography (MRIM), the authors consider that the specificity of contrast-enhanced MRIM (CE MRIM) is greater than that of MRIM without administering magnetic resonance contrast agents (MRCA). CE MRIM reveals clinically suspicious early-stage breast lesions and defines the stage of a tumorous process, the patients being unexposed to ionizing irradiation. CE MRIM as an auxiliary technique becomes one of the most informative ones, particularly in girls, early reproductive-age females with developed glandular tissue, and pregnant females. In the authors' opinion, the total algorithm of a breast study in this group of patients in specialized medical centers where trained physicians work should differ from the generally accepted algorithm: instead of applying X-ray mammography (XRM) as a basic method of primary diagnosis, ultrasound mammography using Doppler color mapping, followed by MRIM or XRM should be employed.

To compare the effects of urological, neurological and obstetricogynecological risk factors of urine incontinence (UI) in women, we made a questionnaire survey covering a representative sample of 4336 female residents of Moscow aged 25-74 years. We used standard epidemiological methods and unified criteria, descriptive statistics and modeling by means of logical regression in 6.12 version of SAS system. We revealed that chronic cystitis and other inflammatory urinary diseases provoke a 4-fold increase in the relative risk (RR) of UI in women being higher than obstetricogynecological risk factors (RR 1.5-2.8) and neurological risk factors (RR 1.3-2.0). UI in many cases is of stress type, but in urinary inflammation a mixed type occurs 1.8 times more frequently. Thus, chronic cystitis and other urinary inflammatory diseases are essential UI risk factors in women.

Reliable correlations between the parameters of mifepriston pharmacokinetics describing the rate of drug elimination from the blood plasma and the levels of beta-human chorionic gonadotropin (beta-HCG) and progesterone reflecting the state of gestation in females have been established fore the first time. According to these relationships, the half elimination time, the mean retention time, and the plasma clearance of mifepriston can be considered as predictors of the clinical efficacy of this drug for the early pregnancy interruption.

The present paper examines the capacities of non-invasive MRI in the diagnosis of endometriosis. A standardized study algorithm is proposed, indications and methodology for MRI in different forms of endometriosis (EM) are specified. It is shown that in the diagnosis of different types of EM there are specific changes in the MR pattern of small pelvic organs, which allow one to make a differential diagnosis of this disease with a higher sensitivity (96%) and specificity (87%) as compared with ultrasound study. MRI makes it possible to assess the degree of invasion of endometrioid heterotopies into the wall of the intestine and cervix uteri with a high degree of accuracy and to judge the degree and extent of intestinal luminal narrowing. Diagnostic errors in solving these problems (as well as those associated with the detection of extragenital foci of EM in the small pelvis) are due first of all to movement artefacts a (respiration and intestinal motility) particularly in the presence of a significant adhesive process and/or after surgical intervention. MRI does not virtually yield false-negative conclusions in the diagnosis of different forms of genital endometriosis (less than 3%), which, in the authors' opinion, rather justifies some hyperdiagnosis (about 11%) made by this method in the diagnosis of endometriosis of the rectovaginal septum. MRI is a closing, specifying stage of instrumental diagnosis of not only EM, but also other small pelvic diseases.

We studied expression of genes of interleukins, interferon-gamma, tumor necrosis factor-alpha, and transforming growth factor-beta(2) in adhesions on uterine tubes. In tubal adhesions the intensity of production of mRNA for proinflammatory cytokines, antiinflammatory cytokine interleukin-10, and regulator of cell proliferation surpassed that in normal tissues by 2.5-7.4, 2.2, and 50.2 times, respectively. Correlations were found between production of mRNA for tumor necrosis factor-alpha and transforming growth factor-beta(2), interleukin-12, and interferon-gamma and interleukin-12 and transforming growth factor-beta(2). Our results suggest that expression of these genes during adhesion formation is regulated by the feedback mechanism.

The paper presents the results of studies of the role of immune factors in the pathogenesis of infertility, impaired gestation, and perinatal abnormalities, which have been performed at the Research Center of Obstetrics, Gynecology, and Perinatology, Russian Academy of Medical Sciences, in the past decade. It gives an evaluation of the contribution of antispermatic antibodies to infertility of unclear genesis, the systemic and local factors of immunity in females with endometriosis, the immune and interferon status in those with sexually transmitted infections, the role of immune factors and intrauterine infection in gestational abnormalities, the involvement of autoimmune mechanisms in reproductive disturbance. The trends of further studies are outlined.

Objective: To investigate the influence of sera and peritoneal fluids (PFs) from fertile and infertile women on the binding of antisperm antibodies to the surface of spermatozoa., Design: The immunoglobulin (Ig) G antisperm antibodies binding to the surface of liver spermatozoa was evaluated after their incubation in antisperm antibodies-positive serum from an infertile male in the presence and absence of female sera or PFs., Setting: Russian Scientific Center for Obstetrics, Gynaecology, and Perinatology., Patient(s): Serum and PF samples from fertile and infertile women; antisperm antibodies-positive serum from infertile men; high-quality fresh semen from healthy donors., Intervention(s): Serum samples were obtained from fertile and infertile women and from infertile men. Peritoneal fluids were collected during routine laparoscopy., Main Outcome Measure(s): The proportion of spermatozoa positive for IgG antibodies and the quantity of antisperm antibodies on the sperm surface measured by flow cytometry (FCM)., Result(s): The addition of sera from fertile or infertile women with endometriosis or pelvic adhesion disease to an IgG antisperm antibodies-positive male serum resulted in significant inhibition of the antisperm antibodies binding to the sperm surface., Conclusion(s): Sera of fertile as well as infertile woman contain factors that block IgG antisperm antibodies binding to the surface of live spermatozoa.

The paper reviews recent-years' papers and the data of their own investigations on a role of obligate anaerobic microbes in obstetric and gynecological abnormality. The fact that secondary pelvic inflammatory processes caused chiefly by non spore-forming anaerobes is ascertained. There is evidence that obligate anaerobic bacteria are involved in the abnormality directly unassociated with the development of an inflammatory process: preterm labor, premature discharge of amniotic fluid, intranatal fetal hypoxia, respiratory distress syndrome and hyaline membrane disease of the premature newborn. Among vaginal infections the key role is played by bacterial vaginosis which is, from the pathophysiological point of view, now a severe derangement of the vaginal environment system with greatly prevalent obligate anaerobic bacteria and without lactoflora. Complications associated with this abnormality are noted.