Search

We present a novel approach for benchmarking and validating quantitative phase tomography (QPT) systems using three-dimensional microphantoms. These microphantoms, crafted from biological and imaging data, replicate the optical and structural properties of multicellular biological samples. Their fabrication featuring refractive index modulation at sub-micrometer details is enabled by two-photon polymerization. We showcase the effectiveness of our technique via a round-robin test of healthy and tumoral liver organoid phantoms across three different QPT systems. This test reveals sample- and system-dependent errors in measuring dry mass and morphology. Tailored microphantoms establish the gold standard for the optimization of hardware setups, reconstruction strategies and error assessment, paving the way for novel non-invasive, label-free measurements of 3D biological samples.

In this paper we present a method to robustly evaluate the quantitative accuracy of various tomographic phase microscopy (TPM) methods with a multiple scattering 3D-printed microphantom with known geometry and refractive index distribution. We demonstrate this method by fabricating a multiple-scattering phantom and comparing 3D refractive index results that are output from three TPM reconstruction methods operating with visible and near-infrared wavelengths. One of these methods assumes the sample to be weak-scattering, while the other two take multiple scattering into account. This study can be readily extended to more complex microphantoms fabricated to more closely capture scattering characteristics of real-world scattering objects, such as tissue.

Objective quality assessment of digital holograms has proven to be a challenging task. While prediction of perceptual quality of the recorded 3D content from the holographic wavefield is an open problem; perceptual quality assessment from content after rendering, requires a time-consuming rendering step and a multitude of possible viewports. In this research, we use 96 Fourier holograms of the recently released HoloDB database to evaluate the performance of well-known and state-of-the-art image quality metrics on digital holograms. We compare the reference holograms with their distorted versions: (i) before rendering on the real and imaginary parts of the quantized complex-wavefield, (ii) after converting Fourier to Fresnel holograms, (iii) after rendering, on the quantized amplitude of the reconstructed data, and (iv) after subsequently removing speckle noise using a Wiener filter. For every experimental track, the quality metric predictions are compared to the Mean Opinion Scores (MOS) gathered on a 2D screen, light field display and a holographic display. Additionally, a statistical analysis of the results and a discussion on the performance of the metrics are presented. The tests demonstrate that while for each test track a few quality metrics present a highly correlated performance compared to the multiple sets of available MOS, none of them demonstrates a consistently high-performance across all four test-tracks., Comment: This research is part of an extensive experiment which includes 3 subjective tests for holographic data. The document related to the first phase of these experiments is accessible via: arXiv:1909.12594 . Also, the holographic database created from these experiments is publicly available via this link: http://ds.erc-interfere.eu/holodb/

Visual quality assessment of digital holograms is facing many challenges. Main difficulties are related to the limited spatial resolution and angular field of view of holographic displays in combination with the complexity of steering and operating them for such tasks. Alternatively, non-holographic displays - and in particular light-field displays - can be utilized to visualize the numerically reconstructed content of a digital hologram. However, their suitability as alternative for holographic displays has not been validated. In this research, we have investigated this issue via a set of comprehensive experiments. We used Fourier holographic principle to acquire a diverse set of holograms, which were either computer-generated from point clouds or optically recorded from real macroscopic objects. A final public data set comprising 96 holograms was created using three compression methods which encoded the holograms at four bit-depths. Three separate subjective-tests were conducted using a holographic display, a light field display and a 2D display. For these subjective experiments, a double stimulus, multi-perspective, multi-depth subjective testing methodology was designed and implemented. The tests show that the non-holographic displays indicate a higher sensitivity to artifacts than the holographic display, though at the same time it is demonstrated they are highly correlated. This indicates that the numerically reconstructed holograms rendered on a light field or 2D display have a high predictive value for the perceived quality on holographic display., Comment: This paper consists of 15 pages