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11 results on '"Kucko, N.W."'

1. EVALUATION OF A CALCIUM PHOSPHATE-COLLAGEN MATRIX BONE GRAFT WITH NEEDLE-SHAPED SUBMICRON SURFACE TOPOGRAPHY IN A CLINICALLY RELEVANT SHEEP POSTEROLATERAL LUMBAR SPINE FUSION MODEL

Author

Kucko, N.W., primary, Crowley, J., additional, Wills, D., additional, Wang, T., additional, Pelletier, M., additional, Yuan, H., additional, Houtzager, G., additional, Campion, C., additional, Walsh, W.R., additional, de Bruijn, J., additional, and Groot, F. Barrère-de, additional

Published
2024
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2. The effect of lipoxin A4 on E. coli LPS-induced osteoclastogenesis

Author

Ali, M., Kucko, N.W., Jansen, J.A., Yang, F., Walboomers, X.F., Ali, M., Kucko, N.W., Jansen, J.A., Yang, F., and Walboomers, X.F.

Abstract

Contains fulltext : 232799.pdf (Publisher’s version ) (Open Access), OBJECTIVES: The objective of the present study was to investigate the effect of lipoxin-type A4 (LXA4) on bacterial-induced osteoclastogenesis. MATERIAL AND METHODS: Human periodontal ligament cells (PDLCs) in coculture with osteoclast precursors (RAW264.7 cells) were exposed to bacterial stimulation with lipopolysaccharide (LPS) to induce inflammation. After 24 h, cells were treated to 100 ng/ml of LXA4 and 50 ng/ml of forymul peptide receptor 2 (FPR2/ALX) receptor antagonist (Boc-2). After 5 days, osteoclastic resorptive activity was assessed on calcium phosphate (CaP) synthetic bone substitute. Additionally, osteoclastic differentiation was evaluated using tartrate-resistant acid phosphatase (TRAP) staining, TRAP enzymatic activity assay, and on the expression of osteoclast-specific genes. RESULTS: We found that stimulation of in the osteoclasts with LPS-stimulated PDLCs induced a significant increase in tartrate-resistant acid phosphatase (TRAP) positive cells, higher resorptive activity, and enhanced expression of specific genes. Meanwhile, LXA4-treatment exhibited strong anti-inflammatory activity, and was able to reverse these inflammatory effects. CONCLUSIONS: We conclude that (1) PDLCs are a potential target for treating bacterial-induced bone resorption in patients with periodontal disease, and (2) LXA4 is a suitable candidate for such therapy. CLINICAL RELEVANCE: The results prove that lipoxins have a protective role in bacterial-induced periodontal inflammation and alveolar bone resorption, which can be translated into a clinical beneficial alterative treatment.

Published
2021

4. Development of Next-Generation Calcium Phosphate Bone Cements with Improved Handling and Mechanical Properties

Author

Kucko, N.W., Leeuwenburgh, S.C.G., Herber, R.P., and Radboud University Nijmegen

Subjects
Radboud Institute for Molecular Life Sciences, Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10], Reconstructive and regenerative medicine [Radboudumc 10]
Abstract

Contains fulltext : 218242.pdf (Publisher’s version ) (Open Access) Radboud University, 26 mei 2020 Promotor : Leeuwenburgh, S.C.G. Co-promotor : Herber, R.P.

Published
2020

5. Micro- and macromechanical characterization of the influence of surface-modification of poly(vinyl alcohol) fibers on the reinforcement of calcium phosphate cements

Author

Kucko, N.W., Petre, D.G., Ruiter, M., Herber, R.P., Leeuwenburgh, S.C.G., Kucko, N.W., Petre, D.G., Ruiter, M., Herber, R.P., and Leeuwenburgh, S.C.G.

Abstract

Contains fulltext : 220688.pdf (Publisher’s version ) (Open Access), Calcium phosphate cements (CPCs) are frequently used as synthetic bone substitute materials due to their favorable osteocompatibility and handling properties. However, CPCs alone are inherently brittle and exhibit low strength and toughness, which restricts their clinical applicability to non-load bearing sites. Mechanical reinforcement of CPCs using fibers has proven to be an effective strategy to toughen these cements by transferring stress from the matrix to the fibers through frictional sliding at the interface. Therefore, tailoring the fiber-matrix affinity is paramount in designing highly toughened CPCs. However, the mechanistic correlation between this interaction and the macromechanical properties of fiber-reinforced CPCs has hardly been investigated to date. The aim of this study was to tailor the fiber-matrix interface affinity by modifying the surface of poly(vinyl alcohol) (PVA) fibers and correlate their interfacial properties to macromechanical properties (i.e. fracture toughness, work-of-fracture and tensile strength) of CPCs. Results from single fiber pullout tests reveal that the surface modification of PVA fibers increased their hydrophilicity and improved their affinity to the CPC matrix. This observation was evidenced by an increase in the interfacial shear strength and a reduction in the critical fiber embedment length (i.e. maximum embedded length from which a fiber can be pulled out without rupture). This increased interface affinity facilitated energy dissipation during fracture of CPCs subjected to macromechanical three-point flexure and tensile tests. The fracture toughness also significantly improved, even for CPCs reinforced with fibers of lengths greater than their critical fiber embedment length, suggesting that other crack-arresting mechanisms also play an important role in mechanically reinforcing CPCs. Overall, these basic insights will improve the understanding of the correlation between micro- and macromechanical characteristics of

Published
2020

8. Sterilization effects on the handling and degradation properties of calcium phosphate cements containing poly ((D,L)-lactic-co-glycolic acid) porogens and carboxymethyl cellulose

Author

Kucko, N.W., Li, Wenliang, Martinez, Marcela A.Garcia, Rehman, Ihtesham ur, Ulset, A.S., Christensen, B.E., Leeuwenburgh, S.C.G., and Herber, R.P.

Subjects
All institutes and research themes of the Radboud University Medical Center, Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]
Abstract

Item does not contain fulltext

Published
2019

11. Long-term evaluation of the degradation behavior of three apatite-forming calcium phosphate cements

Author

An, J., Liao, H., Kucko, N.W., Herber, R.P., Wolke, J.G.C., Beucken, J.J.J.P van den, Jansen, J.A., and Leeuwenburgh, S.C.G.

Subjects
Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10], technology, industry, and agriculture, Other Research Radboud Institute for Molecular Life Sciences [Radboudumc 0], macromolecular substances
Abstract

Item does not contain fulltext Calcium phosphate cements (CPCs) are injectable bone substitutes with a long clinical history because of their biocompatibility and osteoconductivity. Nevertheless, their cohesion upon injection into perfused bone defects as well as their long-term degradation behavior remain major clinical challenges. Therefore, the long-term degradation behavior of two types of alpha-tricalcium phosphate-based, apatite-forming CPCs was compared to a commercially available apatite-forming cement, that is HydroSet(TM) . Carboxyl methylcellulose (CMC) was used as cohesion promotor to improve handling properties of the two experimental cements, whereas poly (d, l-lactic-co-glycolic) acid (PLGA) microparticles were added to introduce macroporosity and stimulate CPC degradation. All three CPCs were injected into defects drilled into rabbit femoral condyles and explanted after 4, 12, or 26 weeks, after which the bone response was assessed both qualitatively and quantitatively. CPCs without PLGA microparticles degraded only at the periphery of the implants, while the residual CPC volume was close to 90%. On the contrary, bone ingrowth was observed not only at the periphery of the CPC, but also throughout the center of the implants after 26 weeks of implantation for the PLGA-containing CPCs with a residual CPC volume of approximately 55%. In conclusion, it was shown that CPC containing CMC and PLGA was able to induce partial degradation of apatite-forming CPCs and concomitant replacement by bone tissue. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1072-1081, 2016. 01 mei 2016

Published
2016

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