20 results on '"Kuckelhaus, Selma A. S."'
Search Results
2. The peptide secreted at the water to land transition in a model amphibian has antioxidant effects
- Author
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Barbosa, Eder Alves, primary, Plácido, Alexandra, additional, Moreira, Daniel C., additional, Albuquerque, Lucas, additional, Dematei, Anderson, additional, Silva-Carvalho, Amandda É., additional, Cabral, Wanessa F., additional, Báo, Sonia N., additional, Saldanha-Araújo, Felipe, additional, Kuckelhaus, Selma A. S., additional, Borges, Tatiana K., additional, Portugal, Camila C., additional, Socodato, Renato, additional, Teixeira, Cátia, additional, Lima, Filipe Camargo D. A., additional, Batagin-Neto, Augusto, additional, Sebben, Antônio, additional, Eaton, Peter, additional, Gomes, Paula, additional, Brand, Guilherme D., additional, Relvas, Joao B., additional, Kato, Massuo J., additional, and Leite, Jose Roberto S. A., additional
- Published
- 2021
- Full Text
- View/download PDF
3. Mechanistic Insights into the Leishmanicidal and Bactericidal Activities of Batroxicidin, a Cathelicidin-Related Peptide from a South American Viper (Bothrops atrox)
- Author
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Dematei, Anderson, primary, Nunes, João B., additional, Moreira, Daniel C., additional, Jesus, Jéssica A., additional, Laurenti, Márcia D., additional, Mengarda, Ana C. A., additional, Vieira, Maria Silva, additional, do Amaral, Constança Pais, additional, Domingues, Marco M., additional, de Moraes, Josué, additional, Passero, Luiz F. D., additional, Brand, Guilherme, additional, Bessa, Lucinda J., additional, Wimmer, Reinhard, additional, Kuckelhaus, Selma A. S., additional, Tomás, Ana M., additional, Santos, Nuno C., additional, Plácido, Alexandra, additional, Eaton, Peter, additional, and Leite, José Roberto S. A., additional
- Published
- 2021
- Full Text
- View/download PDF
4. SUPPLEMENTARY MATERIAL from The peptide secreted at the water to land transition in a model amphibian has antioxidant effects
- Author
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Barbosa, Eder Alves, Pl��cido, Alexandra, Moreira, Daniel C., Albuquerque, Lucas, Dematei, Anderson, Silva-Carvalho, Amandda ��., Cabral, Wanessa F., B��o, Sonia N., Saldanha-Ara��jo, Felipe, Kuckelhaus, Selma A. S., Borges, Tatiana K., Portugal, Camila C., Socodato, Renato, Teixeira, C��tia, Lima, Filipe Camargo D. A., Batagin-Neto, Augusto, Sebben, Ant��nio, Eaton, Peter, Gomes, Paula, Brand, Guilherme D., Relvas, Joao B., and Leite, Jose Roberto S. A.
- Abstract
In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O2 levels and UV exposure on land compared with aquatic environments.
- Published
- 2021
- Full Text
- View/download PDF
5. Quantification of lipid bodies in monocytes from patients with periodontitis
- Author
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Naiff, Priscilla F., primary, Kuckelhaus, Selma A. S., additional, Corazza, Danilo, additional, Leite, Luciana M., additional, Couto, Shirley, additional, deOliveira, Mariangela S., additional, Santiago, Luander M., additional, Silva, Larissa F., additional, Oliveira, Laudimar A., additional, Grisi, Daniela C., additional, Carneiro, Valeria M. A., additional, and Guimarães, Maria do C. M., additional
- Published
- 2020
- Full Text
- View/download PDF
6. The Antioxidant Peptide Salamandrin-I: First Bioactive Peptide Identified from Skin Secretion of Salamandra Genus (Salamandra salamandra)
- Author
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Plácido, Alexandra, primary, Bueno, João, additional, Barbosa, Eder A., additional, Moreira, Daniel C., additional, Dias, Jhones do Nascimento, additional, Cabral, Wanessa Felix, additional, Albuquerque, Patrícia, additional, Bessa, Lucinda J., additional, Freitas, Jaime, additional, Kuckelhaus, Selma A. S., additional, Lima, Filipe C. D. A., additional, Batagin-Neto, Augusto, additional, Brand, Guilherme D., additional, Relvas, João B., additional, Leite, José Roberto S. A., additional, and Eaton, Peter, additional
- Published
- 2020
- Full Text
- View/download PDF
7. Phagocytic activity of monocytes and neutrophils in patients with periodontitis, whether or not associated to type 2 diabetes.
- Author
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Naiff, Priscilla F., Kuckelhaus, Selma A. S., Couto, Shirley, Oliveira, Mariângela, Santiago, Luander M., Cascaes, Andrea C. G., Silva, Larissa F., Oliveira, Laudimar A., Grisi, Daniela C., Carneiro, Valéria M., and Guimarães, Maria do Carmo M.
- Subjects
TYPE 2 diabetes ,PERIODONTITIS ,NEUTROPHILS ,MONOCYTES ,DISEASE complications - Abstract
Copyright of Acta Odontologica Latinoamericana: AOL is the property of Acta Odontologica Latinoamericana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
8. Quantification of lipid bodies in monocytes from patients with periodontitis.
- Author
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Naiff, Priscilla F., Kuckelhaus, Selma A. S., Corazza, Danilo, Leite, Luciana M., Couto, Shirley, deOliveira, Mariangela S., Santiago, Luander M., Silva, Larissa F., Oliveira, Laudimar A., Grisi, Daniela C., Carneiro, Valeria M. A., and Guimarães, Maria do C. M.
- Subjects
PERIODONTITIS ,MONOCYTES - Abstract
Objectives: For the first time in the history of periodontics, the production of lipid bodies by monocytes was assessed from blood of patients with periodontitis in comparison to systemically healthy individuals. The purpose of this study was to compare the lipid body frequency within monocytes between healthy patients and those with periodontal disease. Materials and Methods: A total of 30 participants (11 males and 19 females), were divided between orally healthy control subjects (C, n = 16) and periodontitis subjects (P, n = 14), in a cross‐sectional study. Both groups were systemically healthy. The following clinical periodontal parameters were assessed: probing depth, clinical attachment level, visible plaque index and gingival bleeding on probing index. Blood samples were collected to obtain monocytes containing lipid bodies, which were analyzed by light microscopy. Results: The periodontitis group demonstrated a higher corpuscular index than the control group (nonopsonized p =.0296 or opsonized p =.0459; Mann–Whitney). The frequency of monocyte cells containing lipid bodies (basal p =.0147, opsonized p =.0084 or nonopsonized, p =.026; Mann–Whitney) was also higher compared to those observed in healthy individuals. Conclusions: The data suggest that periodontitis may contribute to a higher production of lipid bodies. It was also hypothesized that a major production of lipid bodies by monocytes in severe periodontitis, compared to orally healthy subjects, could interfere with the innate immune response or represents a higher reservoir of cholesterol esters within macrophages and a major risk to systemic implications, such as atherosclerosis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
9. Epiisopilosine alkaloid has activity against Schistosoma mansoni in mice without acute toxicity
- Author
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Guimarães, Maria A., primary, de Oliveira, Rosimeire N., additional, de Almeida, Rebeca L., additional, Mafud, Ana C., additional, Sarkis, Ana L. V., additional, Ganassin, Rayane, additional, da Silva, Marcos P., additional, Roquini, Daniel B., additional, Veras, Leiz M., additional, Sawada, Tânia C. H., additional, Ropke, Cristina D., additional, Muehlmann, Luis A., additional, Joanitti, Graziella A., additional, Kuckelhaus, Selma A. S., additional, Allegretti, Silmara M., additional, Mascarenhas, Yvonne P., additional, de Moraes, Josué, additional, and Leite, José R. S. A., additional
- Published
- 2018
- Full Text
- View/download PDF
10. Lycopene rich extract from red guava ( Psidium guajava L.) displays anti-inflammatory and antioxidant profile by reducing suggestive hallmarks of acute inflammatory response in mice
- Author
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Vasconcelos, Andreanne G., primary, Amorim, Adriany das G.N., additional, dos Santos, Raimunda C., additional, Souza, Jessica Maria T., additional, de Souza, Luan Kelves M., additional, Araújo, Thiago de S.L., additional, Nicolau, Lucas Antonio D., additional, de Lima Carvalho, Lucas, additional, de Aquino, Pedro Everson A., additional, da Silva Martins, Conceição, additional, Ropke, Cristina D., additional, Soares, Pedro Marcos G., additional, Kuckelhaus, Selma Aparecida S., additional, Medeiros, Jand-Venes R., additional, and Leite, José Roberto de S.A., additional
- Published
- 2017
- Full Text
- View/download PDF
11. Anthelmintic Activity In Vivo of Epiisopiloturine against Juvenile and Adult Worms of Schistosoma mansoni
- Author
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Guimaraes, Maria A., de Oliveira, Rosimeire N., Veras, Leiz M. C., Lima, David F., Campelo, Yuri D. M., Campos, Stefano Augusto, Kuckelhaus, Selma A. S., Pinto, Pedro L. S., Eaton, Peter, Mafud, Ana C., Mascarenhas, Yvonne P., Allegretti, Silmara M., de Moraes, Josue, Lolić, Aleksandar, Verbić, Tatjana, Leite, Jose Roberto S. A., Guimaraes, Maria A., de Oliveira, Rosimeire N., Veras, Leiz M. C., Lima, David F., Campelo, Yuri D. M., Campos, Stefano Augusto, Kuckelhaus, Selma A. S., Pinto, Pedro L. S., Eaton, Peter, Mafud, Ana C., Mascarenhas, Yvonne P., Allegretti, Silmara M., de Moraes, Josue, Lolić, Aleksandar, Verbić, Tatjana, and Leite, Jose Roberto S. A.
- Abstract
Schistosomiasis is a serious disease currently estimated to affect more that 207 million people worldwide. Due to the intensive use of praziquantel, there is increasing concern about the development of drug-resistant strains. Therefore, it is necessary to search for and investigate new potential schistosomicidal compounds. This work reports the in vivo effect of the alkaloid epiisopiloturine (EPI) against adults and juvenile worms of Schistosoma mansoni. EPI was first purified its thermal behavior and theoretical solubility parameters charaterised. In the experiment, mice were treated with EPI over the 21 days post-infection with the doses of 40 and 200 mg/kg, and 45 days post-infection with single doses of 40, 100 and 300 mg/kg. The treatment with EPI at 40 mg/kg was more effective in adult worms when compared with doses of 100 and 300 mg/kg. The treatment with 40 mg/kg in adult worms reduced parasite burden significantly, lead to reduction in hepatosplenomegaly, reduced the egg burden in faeces, and decreased granuloma diameter. Scanning electron microscopy revealed morphological changes to the parasite tegument after treatment, including the loss of important features. Additionally, the in vivo treatment against juvenile with 40 mg/kg showed a reduction of the total worm burden of 50.2%. Histopathological studies were performed on liver, spleen, lung, kidney and brain and EPI was shown to have a DL50 of 8000 mg/kg. Therefore EPI shows potential to be used in schistosomiasis treatment. This is the first time that schistosomicidal in vivo activity of EPI has been reported.
- Published
- 2015
12. Anthelmintic Activity In Vivo of Epiisopiloturine against Juvenile and Adult Worms of Schistosoma mansoni
- Author
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Guimarães, Maria A., primary, de Oliveira, Rosimeire N., additional, Véras, Leiz M. C., additional, Lima, David F., additional, Campelo, Yuri D. M., additional, Campos, Stefano Augusto, additional, Kuckelhaus, Selma A. S., additional, Pinto, Pedro L. S., additional, Eaton, Peter, additional, Mafud, Ana C., additional, Mascarenhas, Yvonne P., additional, Allegretti, Silmara M., additional, de Moraes, Josué, additional, Lolić, Aleksandar, additional, Verbić, Tatjana, additional, and Leite, José Roberto S. A., additional
- Published
- 2015
- Full Text
- View/download PDF
13. Collagen-based silver nanoparticles for biological applications: synthesis and characterization.
- Author
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Cardoso, Vinicius S., Quelemes, Patrick V., Amorin, Adriany, Primo, Fernando Lucas, Gobo, Graciely Gomides, Tedesco, Antonio C., Mafud, Ana C., Mascarenhas, Yvonne P., Corrêa, José Raimundo, Kuckelhaus, Selma A. S., Eiras, Carla, Leite, José Roberto S. A., Silva, Durcilene, and Santos Júnior, José Ribeiro dos
- Subjects
SILVER nanoparticles ,NANOSTRUCTURED materials synthesis ,PHYSIOLOGICAL effects of collagen ,NANOMEDICINE ,FOURIER transform infrared spectroscopy ,ULTRAVIOLET-visible spectroscopy - Abstract
Background Type I collagen is an abundant natural polymer with several applications in medicine as matrix to regenerate tissues. Silver nanoparticles is an important nanotechnology material with many utilities in some areas such as medicine, biology and chemistry. The present study focused on the synthesis of silver nanoparticles (AgNPs) stabilized with type I collagen (AgNPcol) to build a nanomaterial with biological utility. Three formulations of AgNPcol were physicochemical characterized, antibacterial activity in vitro and cell viability assays were analyzed. AgNPcol was characterized by means of the following: ultraviolet-visible spectroscopy, dynamic light scattering analysis, Fourier transform infrared spectroscopy, atomic absorption analysis, transmission electron microscopy and of X-ray diffraction analysis. Results All AgNPcol showed spherical and positive zeta potential. The AgNPcol at a molar ratio of 1:6 showed better characteristics, smaller hydrodynamic diameter (64.34 ± 16.05) and polydispersity index (0.40 ± 0.05), and higher absorbance and silver reduction efficiency (0.645 mM), when compared with the particles prepared in other mixing ratios. Furthermore, these particles showed antimicrobial activity against both Staphylococcus aureus and Escherichia coli and no toxicity to the cells at the examined concentrations. Conclusions The resulted particles exhibited favorable characteristics, including the spherical shape, diameter between 64.34 nm and 81.76 nm, positive zeta potential, antibacterial activity, and non-toxicity to the tested cells (OSCC). [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
14. Development and Antibacterial Activity of Cashew Gum-Based Silver Nanoparticles.
- Author
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Quelemes, Patrick V., Araruna, Felipe B., de Faria, Bruna E. F., Kuckelhaus, Selma A. S., da Silva, Durcilene A., Mendonça, Ronaldo Z., Eiras, Carla, Soares, Maria José dos S., and Leite, José Roberto S. A.
- Subjects
CASHEW tree ,SILVER nanoparticles ,ANTIBACTERIAL agents ,CYTOTOXINS ,TRANSMISSION electron microscopy ,CELL-mediated cytotoxicity ,GRAM-negative bacteria ,ANACARDIUM - Abstract
The present study describes the development of a green synthesis of silver nanoparticles reduced and stabilized by exuded gum from Anacardium occidentale L. and evaluates in vitro their antibacterial and cytotoxic activities. Characterization of cashew gum-based silver nanoparticles (AgNPs) was carried out based on UV--Vis spectroscopy, transmission electron microscopy and dynamic light scattering analysis which revealed that the synthesized silver nanoparticles were spherical in shape, measuring about 4 nm in size with a uniform dispersal. AgNPs presented antibacterial activity, especially against Gram-negative bacteria, in concentrations where no significant cytotoxicity was observed. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
15. Promising self-emulsifying drug delivery system loaded with lycopene from red guava (Psidium guajavaL.): in vivo toxicity, biodistribution and cytotoxicity on DU-145 prostate cancer cells
- Author
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Vasconcelos, Andreanne G., Barros, Ana Luisa A. N., Cabral, Wanessa F., Moreira, Daniel C., da Silva, Ingrid Gracielle M., Silva-Carvalho, Amandda É., de Almeida, Miguel P., Albuquerque, Lucas F. F., dos Santos, Raimunda C., S. Brito, Ana Karolinne, Saldanha-Araújo, Felipe, Arcanjo, Daniel D. R., C. Martins, Maria do Carmo, dos S. Borges, Tatiana K., Báo, Sônia N., Plácido, Alexandra, Eaton, Peter, Kuckelhaus, Selma A. S., and Leite, José Roberto S. A.
- Abstract
Background: Self-emulsifying drug delivery systems (SEDDSs) have attracted attention because of their effects on solubility and bioavailability of lipophilic compounds. Herein, a SEDDS loaded with lycopene purified from red guava (nanoLPG) was produced. The nanoemulsion was characterized using dynamic light scattering (DLS), zeta potential measurement, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), lycopene content quantification, radical scavenging activity and colloidal stability in cell culture medium. Then, in vivo toxicity and tissue distribution in orally treated mice and cytotoxicity on human prostate carcinoma cells (DU-145) and human peripheral blood mononuclear cells (PBMC) were evaluated. Results: NanoLPG exhibited physicochemical properties with a size around 200 nm, negative zeta-potential, and spherical morphology. The size, polydispersity index, and zeta potential parameters suffered insignificant alterations during the 12 month storage at 5 °C, which were associated with lycopene stability at 5 °C for 10 months. The nanoemulsion showed partial aggregation in cell culture medium at 37 °C after 24 h. NanoLPG at 0.10 mg/mL exhibited radical scavenging activity equivalent to 0.043 ± 0.002 mg Trolox/mL. The in vivo studies did not reveal any significant changes in clinical, behavioral, hematological, biochemical, and histopathological parameters in mice orally treated with nanoLPG at 10 mg/kg for 28 days. In addition, nanoLPG successfully delivered lycopene to the liver, kidney and prostate in mice, improved its cytotoxicity against DU-145 prostate cancer cells—probably by pathway independent on classical necrosis and apoptosis—and did not affect PBMC viability. Conclusions: Thus, nanoLPG stands as a promising and biosafe lycopene delivery system for further development of nanotechnology-based health products. Graphical Abstract:
- Published
- 2021
- Full Text
- View/download PDF
16. Structure–Activity Relationship of Piplartine and Synthetic Analogues against Schistosoma mansoni and Cytotoxicity to Mammalian Cells.
- Author
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Campelo, Yuri, Ombredane, Alicia, Vasconcelos, Andreanne G., Albuquerque, Lucas, Moreira, Daniel C., Plácido, Alexandra, Rocha, Jefferson, Hilarion Fokoue, Harold, Yamaguchi, Lydia, Mafud, Ana, Mascarenhas, Yvonne P., Delerue-Matos, Cristina, Borges, Tatiana, Joanitti, Graziella A., Arcanjo, D. R. Daniel, Kato, Massuo J., Kuckelhaus, Selma A. S., Silva, Marcos P. N., Moraes, Josué de, and Leite, José Roberto S. A.
- Subjects
SCHISTOSOMIASIS ,HELMINTHIASIS ,HELMINTHS ,PRAZIQUANTEL ,FIBROBLASTS ,THERAPEUTICS - Abstract
Schistosomiasis, caused by helminth flatworms of the genus
Schistosoma , is an infectious disease mainly associated with poverty that affects millions of people worldwide. Since treatment for this disease relies only on the use of praziquantel, there is an urgent need to identify new antischistosomal drugs. Piplartine is an amide alkaloid found in severalPiper species (Piperaceae) that exhibits antischistosomal properties. The aim of this study was to evaluate the structure–function relationship between piplartine and its five synthetic analogues (19A, 1G, 1M, 14B and 6B) againstSchistosoma mansoni adult worms, as well as its cytotoxicity to mammalian cells using murine fibroblast (NIH-3T3) and BALB/cN macrophage (J774A.1) cell lines. In addition, density functional theory calculations and in silico analysis were used to predict physicochemical and toxicity parameters. Bioassays revealed that piplartine is active againstS. mansoni at low concentrations (5–10 µM), but its analogues did not. In contrast, based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, piplartine exhibited toxicity in mammalian cells at 785 µM, while its analogues 19A and 6B did not reduce cell viability at the same concentrations. This study demonstrated that piplartine analogues showed less activity againstS. mansoni but presented lower toxicity than piplartine. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
17. The peptide secreted at the water to land transition in a model amphibian has antioxidant effects.
- Author
-
Barbosa EA, Plácido A, Moreira DC, Albuquerque L, Dematei A, Silva-Carvalho AÉ, Cabral WF, Báo SN, Saldanha-Araújo F, Kuckelhaus SAS, Borges TK, Portugal CC, Socodato R, Teixeira C, Lima FCDA, Batagin-Neto A, Sebben A, Eaton P, Gomes P, Brand GD, Relvas JB, Kato MJ, and Leite JRSA
- Subjects
- Animals, Anura physiology, Humans, Mammals, Peptides analysis, Skin, Antioxidants analysis, Water analysis
- Abstract
In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus , right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O
2 levels and UV exposure on land compared with aquatic environments.- Published
- 2021
- Full Text
- View/download PDF
18. Structure⁻Activity Relationship of Piplartine and Synthetic Analogues against Schistosoma mansoni and Cytotoxicity to Mammalian Cells.
- Author
-
Campelo Y, Ombredane A, Vasconcelos AG, Albuquerque L, Moreira DC, Plácido A, Rocha J, Hilarion Fokoue H, Yamaguchi L, Mafud A, Mascarenhas YP, Delerue-Matos C, Borges T, Joanitti GA, Arcanjo D, Kato MJ, Kuckelhaus SAS, Silva MPN, Moraes J, and Leite JRSA
- Subjects
- 3T3 Cells, Animals, Anthelmintics chemistry, Anthelmintics toxicity, Cricetinae, Fibroblasts drug effects, Macrophages drug effects, Mice, Mice, Inbred BALB C, Piper chemistry, Piperidones chemistry, Piperidones toxicity, Plant Extracts chemistry, Plant Extracts toxicity, Quantitative Structure-Activity Relationship, Snails, Anthelmintics pharmacology, Piperidones pharmacology, Plant Extracts pharmacology, Schistosoma mansoni drug effects
- Abstract
Schistosomiasis, caused by helminth flatworms of the genus Schistosoma , is an infectious disease mainly associated with poverty that affects millions of people worldwide. Since treatment for this disease relies only on the use of praziquantel, there is an urgent need to identify new antischistosomal drugs. Piplartine is an amide alkaloid found in several Piper species (Piperaceae) that exhibits antischistosomal properties. The aim of this study was to evaluate the structure–function relationship between piplartine and its five synthetic analogues (19A, 1G, 1M, 14B and 6B) against Schistosoma mansoni adult worms, as well as its cytotoxicity to mammalian cells using murine fibroblast (NIH-3T3) and BALB/cN macrophage (J774A.1) cell lines. In addition, density functional theory calculations and in silico analysis were used to predict physicochemical and toxicity parameters. Bioassays revealed that piplartine is active against S. mansoni at low concentrations (5⁻10 µM), but its analogues did not. In contrast, based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, piplartine exhibited toxicity in mammalian cells at 785 µM, while its analogues 19A and 6B did not reduce cell viability at the same concentrations. This study demonstrated that piplartine analogues showed less activity against S. mansoni but presented lower toxicity than piplartine.
- Published
- 2018
- Full Text
- View/download PDF
19. Morphometry of latent palmprints as a function of time.
- Author
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Barros RM, Faria BE, and Kuckelhaus SA
- Subjects
- Adult, Female, Humans, Linear Models, Male, Time Factors, Young Adult, Dermatoglyphics, Hand anatomy & histology, Skin ultrastructure
- Abstract
In many crimes, the elapsed time between production and collecting fingermark traces is crucial. and a method able to detect the aging of latent prints would represent an improvement in forensic procedures. Considering that as the latent print gets older, substantial changes in the relative proportion of individual components secreted by skin glands could affect the morphology of ridges, morphometry could be a potential tool to assess the aging of latent fingermarks. Then, considering the very limited research in the field, the present work aims to evaluate the morphometry of latent palmprint ridges, as a function of time, in order to identify an aging pattern. The latent marks were deposited by 20 donors on glass microscope slides considering pressure and contact angle, and then were maintained under controlled environmental conditions. The morphometric study was conducted on marks developed with magnetic powder in 7 different time intervals after deposition (0, 5, 10, 15, 20, 25 or 30 days); 60 ridges were evaluated for each developed mark. The results showed that: 1) the method for the replacement and mixing of skin secretions on the palm was appropriate to ensure reproducibility of latent prints, and 2) considering the studied group, there was a time-dependent reduction in the width of ridges and on the percentage of visible ridges over 30 days. Results suggest the possibility of using the morphometric method to determine an aging profile of latent palmprints on glass surface, aiming for forensic purposes., (© 2013.)
- Published
- 2013
- Full Text
- View/download PDF
20. Novel dermaseptins from Phyllomedusa hypochondrialis (Amphibia).
- Author
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Brand GD, Leite JR, de Sá Mandel SM, Mesquita DA, Silva LP, Prates MV, Barbosa EA, Vinecky F, Martins GR, Galasso JH, Kuckelhaus SA, Sampaio RN, Furtado JR Jr, Andrade AC, and Bloch C Jr
- Subjects
- Amino Acid Sequence, Amphibian Proteins chemistry, Amphibian Proteins isolation & purification, Amphibian Proteins pharmacology, Animals, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents metabolism, Anti-Bacterial Agents pharmacology, Antimicrobial Cationic Peptides chemistry, Antimicrobial Cationic Peptides isolation & purification, Antimicrobial Cationic Peptides pharmacology, Humans, Leishmania drug effects, Leukocytes drug effects, Molecular Sequence Data, Peptide Fragments chemistry, Peptide Fragments isolation & purification, Peptide Fragments metabolism, Peptide Fragments pharmacology, Pseudomonas aeruginosa drug effects, Sequence Alignment, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staphylococcus aureus drug effects, Surface Plasmon Resonance, Amphibian Proteins metabolism, Antimicrobial Cationic Peptides metabolism, Anura metabolism
- Abstract
Six new antimicrobial peptides structurally related to the dermaseptin family have been isolated from the skin secretion of the amphibian Phyllomedusa hypochondrialis. The primary structures of these molecules named as DShypo 01, 02, 03, 04, 06, and 07 were determined by de novo MS/MS experiments, Edman degradation, and cDNA sequencing. The fifth peptide was found to be precisely the same DS 01 from Phyllomedusa oreades previously described by our group. The majority of the peptides purified from the crude skin secretion could be directly localized and mapped onto a freshly dissected dorsal skin fragment using mass spectrometry-imaging techniques. Comparisons between peptides and commercial drugs on their antibacterial and anti-Leishmania amazonensis efficiencies, associated with peptide lytic effects on mammalian blood cells and surface plasmon resonance interaction studies on immobilized DMPC vesicles, were also performed.
- Published
- 2006
- Full Text
- View/download PDF
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