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The aim of this study was to check whether honey bee drones are also injured. We also compared the degree of injury to drones in own and stranger colonies. Drones were stored in mailing cages in their own colonies and stranger colonies. The number of injuries and the death rate were checked twice, after 3 and 7 days of storage. In total, over 4,608 drones were examined. Nine different types of injuries were observed for the drones, with leg injuries being the most common - lack of segments of tarsus (ca. 70-75% of all injuries). Other types of injuries included black arolia, missing arolia, wing and antenna injuries. The research showed that drones stored in bee colonies suffer injuries just like queens and worker bees do, though to a significantly lesser extent. This study also showed that storage of drones in mailing cages resulted in very high mortality of 62 to 75%. [ABSTRACT FROM AUTHOR]

The Rhizoctonia solani species consists of multinucleate isolates that belong to anastomosis groups AG1–AG3 and differ in virulence and host affinity. R. cerealis is a binucleate species of anastomosis group AG-D which causes sharp eyespot, a common plant disease in Poland. Rhizoctonia spp. is a ubiquitous soil pathogen that poses a significant threat for global crop production due to the absence of effective crop protection products. The aim of this study was to determine the virulence of R. solani and R. cerealis isolates towards Beta vulgaris, Zea mays, Triticum spelta and T. aestivum seedlings, to confirm the presence of endopolygalacturonase genes pg1 and pg5 in the genomes of the tested isolates and to evaluate the tested isolates’ sensitivity to triazole, strobilurin, imidazole and carboxamide fungicides. All tested isolates infected B. vulgaris seedlings. but none of them were virulent against Z. mays plants. R. solani isolates AG4 PL and AG2-2IIIB PL were characterized by the highest virulence (average infestation score of 2.37 and 2.53 points on a scale of 0–3 points) against sugar beet seedlings. The prevalence of infections caused by most of the analysed isolates (in particular R. solani AG4 J—11.8, and R. cerealis RC2—0.78) was higher in spelt than in bread wheat. The virulence of the analysed isolates was not correlated with the presence of pg1 and pg5 genes. The efficacy of the tested fungicides in controlling Rhizoctonia spp. infections was estimated at 100% (propiconazole + cyproconazole), 98.8% (penthiopyrad), 95.4% (tebuconazole) and 78.3% (azoxystrobin). [ABSTRACT FROM AUTHOR]

An increasing number of studies show that urbanized areas are habitats of high biological value and ecological significance. Most bee species live in areas altered by man, either in cities - fragmented urban habitats - or in large rural monocultures. Our research is based on three-year observations of population development of the solitary bee Osmia rufa L. in three habitat types: city, suburbs and villages. We compared reproductive parameters and diversity of accompanying nest fauna. Population growth rate was high in all habitats, exceeding five times the number of cocoons placed in the previous year. We found no significant differences in the number of cocoons and brood mortality between areas with different urbanization levels. In nests located in suburbs, parasites and cleptoparasites occupied almost three times more nest chambers than in other habitats. Changes in the habitat structure had a significant impact on the diversity and abundance of accompanying fauna. Our study shows that red mason bees are flexible and easily adapt to new conditions, despite changes caused by urbanization and agriculture.

Introduction: Social cognitive deficits (e.g. emotion perception, empathy, and theory of mind) seem to play crucial role in the symptoms persistence of anorexia nervosa (AN). Objectives: Aim of study was to verify the therapeutic efficacy of cultural adaptation of Social Cognitive Training (SCT) in socioemotional improvement of patients with AN. Methods: Participants. The participants were 60 healthy controls (HCs) and 60 AN patients, examined at the beginning and at the end of 12-week-long hospitalization. Half of them was offered treatment as usual (TAU) while the other half -TAU + SCT. Measures. The clinical variables were measured using: The Rosenberg Self-Esteem Scale, The Eating Attitude Test, The Beck Depression Inventory, The State-Trait Anxiety Inventory, and The Toronto Alexithymia Scale (TAS-20). Social cognitive deficits were assessed using: Penn Emotion Recognition/Discrimination, and Acuity Tasks, Penn Facial Memory, and Reading the Mind in the Eyes Test (RME). Results: At the beginning both groups of patients presented with high level of clinical psychopathology and social cognitive deficits which decreased significantly throughout therapy however most of the measured variables did not reach the level presented by HCs, apart from: sad recognition, reaction time for happy recognition, emotion discrimination time for happy and sad in TAU + SCT group. Overall, TAU + SCT group showed significantly higher improvement compared to the TAU group in: sad recognition, fear and anger recognition, sad discrimination, RT happy recognition, and ToM after treatment. Conclusions: SCT appeared promising therapeutic tool in remediation of social cognitive functioning in AN. Project funded by National Science Centre NCN 2014/15/B/HS6/01847. [ABSTRACT FROM AUTHOR]

Ewelina Wilkos,2 Timothy JB Brown,3 Ksenia Slawinska,1 Katarzyna A Kucharska2,3 1Department of Neurology, 2Department of Neuroses, Personality and Eating Disorders Institute of Psychiatry and Neurology, Warsaw, Poland; 3Department of Medical Education, Hull York Medical School, Hull, UK Background: The essential role of the thalamus in neurocognitive processes has been well documented. In contrast, relatively little is known about its involvement in social cognitive processes such as recognition of emotion, mentalizing, or empathy. The aim of the study: This study was designed to compare the performance of eight patients (five males, three females, mean age ± SD: 63.7±7.9 years) at early stage of unilateral thalamic lesions and eleven healthy controls (six males, five females, 49.6±12.2 years) in neurocognitive tests (CogState Battery: Groton Maze Learning Test, GML; Groton Maze Learning Test-Delayed Recall, GML-DR; Detection Task, DT; Identification Task, IT; One Card Learning Task, OCLT; One Back Task, OBT; Two Back Task, TBT; Set-Shifting Task, S-ST) and other well-known tests (Benton Visual Retention Test, BVRT; California Verbal Learning Test, CVLT; The Rey-Osterrieth Complex Figure Test, ROCF; Trail Making Test, TMT part A and B; Color – Word Stroop Task, CWST; Verbal Fluency Test, VFT), and social cognitive tasks (The Penn Emotion Recognition Test, ER40; Penn Emotion Discrimination Task, EmoDiff40; The Penn Emotional Acuity Test, PEAT40; Reading the Mind in the Eyes Test, revised version II; Toronto Alexithymia Scale, TAS-20). Methods: Thalamic-damaged subjects were included if they experienced a single-episode ischemic stroke localized in right or left thalamus. The patients were examined at 3 weeks after the stroke onset. All were right handed. In addition, the following clinical scales were used: the Mini-Mental State Examination (MMSE), Spielberger State-Trait Anxiety Inventory (STAI), Beck Depression Inventory (BDI II). An inclusion criteria was a minimum score of 23/30 in MMSE. Results: Compared with the healthy controls, patients revealed significantly lower scores in CVLT, GML-DR, and VFT. Furthermore, compared to healthy controls, patients showed significantly delayed recognition of “happiness” in EmoDiff40 and significantly worse performance on Reading the Mind in the Eyes Test, revised version II. Neuropsychological assessment demonstrated some statistically significant deficits in learning and remembering both verbal and visual material, long-term information storing, problem solving, and executive functions such as verbal fluency. Conclusion: Patients at early stage of unilateral thalamic stroke showed both neurocognitive and social cognitive deficits. Further research is needed to increase understanding about diagnosis, early treatment, and prognosis of patients with thalamic lesions. Keywords: social cognitive deficits, neurocognitive deficits, thalamic stroke, posterior, inferolateral, paramedian

Introduction: Current research emphasizes the significant role of cognitive deficits (especially in attention, cognitive flexibility, central coherence, visuospatial, and executive functions) play in the illness course and symptoms persistence. Objectives: Aim of study was to verify the therapeutic efficacy of cultural adaptation of Neurocognitive Training (NT) in cognitive remediation in AN patients. Methods: Participants. The participants were 60 healthy controls (HCs) and 60 AN patients, examined at the beginning and at the end of 12-week-long hospitalization. Half of them was offered treatment as usual (TAU) while the other half -TAU + NT. Measures. The clinical variables were measured using: The Rosenberg Self-Esteem Scale, The Eating Attitude Test, The Beck Depression Inventory, The State-Trait Anxiety Inventory, and The Toronto Alexithymia Scale. Cognitive deficits were measured using: The Ruff Figural Fluency Test (RFFT), The Trail Making Test (TMT), The Stroop Test (TS), The Rey-Osterrieth Complex Figure Test (ROCF), and The Verbal Fluency Test (VFT). Results: At the beginning both AN groups presented with bigger cognitive deficits which decreased significantly throughout therapy however most of the measured variables did not reach the level presented by HCs. NT+TAU showed significantly higher improvement compared to TAUs and some measured variables even reached the healthy level, as follows: TMT-A(time), TS black (time), RFFT, ROCF, VFT(errors). Moreover, greater improvement incognitive functioning co-occurred with greater improvement of clinical variables. Conclusions: AN is characterized by neurocognitive deficits responsive to cognitive remediation program. Project funded by National Science Centre NCN 2014/15/B/HS6/01847. [ABSTRACT FROM AUTHOR]

The removal of benzene, toluene, ethylbenzene, and xylene (BTEX) from air was investigated in two similar biotrickling filters (BTFs) packed with polyurethane (PU) foam, differing in terms of inoculation procedure (BTF A was packed with pre-incubated PU discs, and BTF B was inoculated via the continuous recirculation of a liquid inoculum). The effects of white rot fungi enzyme extract addition and system responses to variable VOC loading, liquid trickling patterns, and pH were studied. Positive effects of both packing incubation and enzyme addition on biotrickling filtration performance were identified. BFF A exhibited a shorter start-up period (approximately 20 days) and lower pressure drop (75 ± 6 mm H 2 O) than BTF B (30 days; 86 ± 5 mm H 2 O), indicating the superior effects of packing incubation over inoculum circulation during the biotrickling filter start-up. The novel approach of using white rot fungi extracts resulted in fast system recovery and enhanced process performance after the BTF acidification episode. Average BTEX elimination capacities of 28.8 ± 0.4 g/(m 3 h) and 23.1 ± 0.4 g/(m 3 h) were reached for BTF A and BTF B, respectively. This study presents new strategies for controlling and improving the abatement of BTEX in biotrickling filters.

Diffusive motion accompanies many physical and biological processes. The Stokes-Sutherland-Einstein relation for the translational diffusion coefficient, D T , agrees with experiments done in simple fluids but fails for complex fluids. Moreover, the interdependence between D T and rotational diffusion coefficient, D R , also deviates in complex fluids from the classical relation of D T / D R = 4 r 2 /3 known in simple fluids. Makuch et al. Soft Matter , 2020, 16 , 114-124 presented a generalization of the classical translational and rotational diffusion theory for complex fluids. In this work, we empirically verify this model based on simultaneous translational and rotational diffusion measurements. We use fluorescently stained cowpea chlorotic mottle virus (CCMV) particles as monodisperse probes and aqueous polyethylene glycol (PEG) solutions as a model complex fluid. The theory and experimental data obtained from fluorescence correlation spectroscopy (FCS) measurements agreed. Finally, we used the same model and analyzed the diffusion of Yo-Pro-1 stained large ribosomal subunits (LSU) in the cytoplasm and nucleus of living HeLa cells.

Young birds at an intensive growth stage are especially vulnerable to the deleterious effects of contaminants such as lead (Pb). This element negatively influences organs, development and even behavior, which can consequently lead to lower survival and reproductive success of the population. There was a suspicion that these mechanisms, along with other factors, retarded population growth of the Black Stork - still a heavily understudied species in respect to pollution, especially Pb. Therefore, we undertook a study concerning Pb concentrations in the down and blood of Black Stork nestlings from breeding grounds in central and southern Poland (Europe). To investigate the effects of Pb exposure on nestling condition, scale mass index and reduced glutathione levels were also studied, but results indicated that Pb did not influence either of these parameters. The highest blood Pb concentration reached 0.247 μg/g, while the highest down Pb concentrations was significantly higher reaching 4.95 μg/g. Concentrations in blood and down were not correlated with each other. Nest location and year of sampling were not influential for blood but were influential for down Pb concentrations. Relationships between Pb concentrations, habitat characteristics and proximity to emitters were not significant. Overall Pb concentrations in Black Stork nestlings were below the toxicity threshold and did not have negative effects on specimens studied. They were probably related to maternal exposure during migration, on stopover and breeding grounds as well as with food provided by parents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Background: Self-disgust is a negative self-conscious emotion, which has been linked with borderline personality disorder (BPD). However, it has not yet been investigated in relation to both emotion dysregulation and alexithymia, which are recognized as crucial to BPD. Therefore, the aim of our study was to measure these variables and examine the possible mediational role of emotional alterations and comorbid anxiety and depression symptoms in shaping self-disgust in patients with BPD and healthy controls (HCs)., Methods: In total, the study included 100 inpatients with BPD and 104 HCs. Participants completed: the Self-Disgust Scale (SDS), Disgust Scale - Revised (DS-R), Toronto Alexithymia Scale (TAS-20), Emotion Dysregulation Scale short version (EDS-short), Borderline Personality Disorder Checklist (BPD Checklist), State-Trait Anxiety Inventory (STAI), and Center for Epidemiologic Studies Depression Scale (CESD-R)., Results: Inpatients with BPD showed higher self-disgust, alexithymia, emotion dysregulation, core and comorbid symptoms levels, and lower disgust sensitivity. Alexithymia, emotion dysregulation, and trait anxiety partially mediated between BPD diagnosis and self-disgust. The relationship between the severity of BPD symptoms and self-disgust was fully mediated by alexithymia, emotion dysregulation, depressive symptoms, and trait anxiety., Conclusions: The results of our study may imply the contribution of emotion dysregulation, alexithymia, and comorbid psychopathology to self-referenced disgust in BPD., (© 2023. BioMed Central Ltd., part of Springer Nature.)

Objectives: The aim of the study was to assess the presence of symptoms of sexuality-related disorders (S-RD) in a group of overweight and obese women and the relationship of these symptoms with maladaptive attitudes and beliefs about food, the severity of anxiety and depressive symptoms, alcohol consumption, difficulties in emotional regulation, and the general quality of life., Methods: The study group (SG) consisted of 44 overweight/obese women, the control group (CG) consisted of 51 women with normal body weight, all aged 18-40 years. The following were used: Sexological Questionnaire, Eating Attitude Test, Eating Beliefs Questionnaire, Alcohol Use Disorder Identification Test, Hospital Anxiety and Depression Scale, and the Quality of Life Questionnaire., Results: SG showed more severe S-RD symptoms, including sexual dysfunctions and sexual preferences disorders, as compared to CG. In the SG there were more significant correlations between S-RD and the studied variables, especially in the area of difficulties with emotional regulation. Negative and permissive dietary beliefs were significant for S-RD in SG, while positive beliefs were significant in CG. Additionally, the number of S-RD symptoms predicted the quality of life., Conclusions: e results indicate a relationship between excessive body weight and greater severity of S-RD symptoms, as well as a relationship between the symptoms of S-RD with clinical variables and with the quality of life. Further research exploring the mechanisms of the observed relationships is necessary.

This review aims to summarise the current knowledge on structural brain changes among people diagnosed with gaming disorder and the resulting clinical implications. The review will show the theoretical psychological and neurobiological models of computer gaming disorder in conjunction with the results of structural neuroimaging studies. Previous epidemiological studies indicate that the prevalence of gaming disorder in the population may reach approx. 2%. Researchers indicate that the aetiopathogenesis of computer game use disorder is complex and includes psychological, social, as well as neurological and hormonal factors. From the perspective of psychological research exploring gaming disorder, it can be concluded that a person has certain specific psychopathological features and/or symptoms, which, through mediating factors, such as the inability to cope with stress or negative emotions, influence the formation of the symptoms of the disorder. In the context of the neurobiology of behavioural addictions, researchers point to disorders in the mesocorticolimbic reward system, which is influenced by dysfunctional neuronal mechanisms of emotion and stress regulation. When describing structural changes in the brain, researchers most often report differences in the volume of grey matter, which include areas of the dorsolateral prefrontal cortex, temporooccipital cortex, superior and posterior parietal cortex, anterior cingulate cortex, cerebellum, insular cortex, limbic system, and basal ganglia.

Introduction: Health care professionals cover the front line in the battle against infectious diseases. Vaccination of healthcare workers represents a vital element of the strategy for enhancing epidemic safety and improving the quality of health care, inter alia, by limiting microorganism transmission, and reducing patient morbidity and mortality., Method: The study group consisted of all employees of the long-term health care centre in Lublin, Poland. Participants were requested to complete a questionnaire prepared for the purpose of the study, along with a mandatory COVID-19 vaccination interview questionnaire., Results: The vaccination coverage rate for the group of medical workers was 77.3% and of non-medical 86%. The most frequently indicated sources of information on vaccination were the mass media. Males more often than females used the press as a source of information on vaccination. Scientific articles were the most common source of information for both people with higher education and medical professionals. The most common motive for vaccination for females is concern for the health of one's family, and for HCWs and people with at least secondary education-concern for the health of patients., Conclusion: At least one assessed factor influences the decision to be vaccinated. The mass media are of the greatest importance in obtaining information about vaccination., (© The Author(s) 2021. Published by Oxford University Press on behalf of Faculty of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

(1) Background: Body image is being defined as the picture of our own body in our mind with its size and shape, and with a perceptive and attitudinal evaluation of this body. It appears to be a complex clinical construct predisposing an individual to developing and maintaining anorexia nervosa (AN), as well as having considerable impact on prolonging the duration of this illness and its relapse risk. The aim of the research work was to assess whether the symptomatology of eating disorders, level of depression, and mental pain are associated with body image, and examine the influence of a distorted body image as well as eating disorders and depression symptoms on mental pain in AN; (2) Methods: A total of 36 women diagnosed with AN and 69 healthy controls (HC) participated in this study. All participants completed a battery of the following scales: EAT-26, BSQ-34, BIDQ, BDD-YBOCS, CESDR, and the Mental Pain Scale; (3) Results: Results show statistically significantly greater body image disturbances and higher level of depression and mental pain intensity in the AN compared to the HC group. Regression analysis indicates a greater impact of distorted body image, eating disorders, and depression symptoms on mental pain in AN; (4) Conclusions: As assumed, distorted body image and mental pain are central components of AN that should be especially emphasized in the therapeutical process of treating AN. Future research should focus on the etiopathogenesis of distorted body image in relation to the chronicity of mental pain and depression in AN, and address these outcomes in clinical practice to minimize suicide risk in this high-risk group of patients.

Using bioconversion and simultaneous value-added product generation requires purification of the gaseous and the liquid streams before, during, and after the bioconversion process. The effect of diversified process parameters on the efficiency of biohydrogen generation via biological processes is a broad object of research. Biomass-based raw materials are often applied in investigations regarding biohydrogen generation using dark fermentation and photo fermentation microorganisms. The literature lacks information regarding model mixtures of lignocellulose and starch-based biomass, while the research is carried out based on a single type of raw material. The utilization of lignocellulosic and starch biomasses as the substrates for bioconversion processes requires the decomposition of lignocellulosic polymers into hexoses and pentoses. Among the components of lignocelluloses, mainly lignin is responsible for biomass recalcitrance. The natural carbohydrate-lignin shields must be disrupted to enable lignin removal before biomass hydrolysis and fermentation. The matrix of chemical compounds resulting from this kind of pretreatment may significantly affect the efficiency of biotransformation processes. Therefore, the actual state of knowledge on the factors affecting the culture of dark fermentation and photo fermentation microorganisms and their adaptation to fermentation of hydrolysates obtained from biomass requires to be monitored and a state of the art regarding this topic shall become a contribution to the field of bioconversion processes and the management of liquid streams after fermentation. The future research direction should be recognized as striving to simplification of the procedure, applying the assumptions of the circular economy and the responsible generation of liquid and gas streams that can be used and purified without large energy expenditure. The optimization of pre-treatment steps is crucial for the latter stages of the procedure.

Altered emotion dynamics and emotion regulation (ER) have been indicated in theoretical descriptions of abnormal emotional functioning, which contributes to the development and maintenance of anorexia nervosa (AN). Ecological momentary assessment (EMA) has recently become popular in research on eating disorders. It is a source of new insights into the psychopathology of AN as it enables intensive long-term tracking of everyday experiences and behaviours of individuals through repeated self-reports. The following systematic review aims to synthesize research on the use of EMA when evaluating emotion dynamics and ER in AN. Specific studies were identified with the use of MEDLINE, PsycINFO, and Scopus databases. A supplemental search was performed in reference lists of the relevant publications. As a result, 27 publications were identified and included in the systematic review. The findings from the reviewed studies point to various disturbed components of emotion dynamics as well as to unique associations of maladaptive ER strategies with specific abnormalities in emotion dynamics in AN. Limitations of the studies were discussed as well. An outlook for further research in the field was provided in the last section of the paper.

Lead (Pb) is a highly toxic pollutant and represents a serious threat to wildlife, affecting various systems in animal bodies. Especially prone to Pb poisoning are waterbirds, which may inadvertently ingest spent gunshot, fishing sinkers and contaminated sediments. This research thus focused on evaluating Pb concentrations in the blood of 170 Mute swans (Cygnus olor; a widespread species of waterbirds) at their summer (urban locations in Małopolskie and rural locations in Świętokrzyskie regions) and winter (the urban section of Wisła River in Krakow) sites in Poland (Europe). The study concentrated on comparing blood Pb concentrations according to sites and locations, verifying the influence of sex and age factors, examining the impact on hematocrit (Ht), glutathione (GSH) levels and body condition. Mean blood Pb concentrations (measured with ICP-OES) differed significantly between summer and winter sites (Regression by Maximum Likelihood Estimation (RML), p < 0.001) reaching higher values at winter sites. Concentrations did not differ from year to year (RML, p = 0.028). At summer sites, concentrations differed from location to location: swans from urban locations in Małopolskie had higher blood Pb concentrations (0.055 μg/g) than from rural Świętokrzyskie (0.008 μg/g; RML, p < 0.001). In summer and winter birds, neither sex nor age groups differed significantly the concentrations (RML, p > 0.231). Pb concentrations correlated weakly with Ht and GSH levels (Spearman test) and had no influence on body condition (proxied by scaled mass index; GLM, p = 0.246). We concluded that differences between summer and winter sites were dictated mainly by the type of habitat (rural vs. urban) that birds occupied in different seasons., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Haplothrips niger is recognized as a parthenogenetic form of H. leucanthemi and is also considered to be a pest in clover-seed plantations. On the contrary, some researchers highlight the distinctiveness of H. niger and H. leucanthemi . Taking into account these two points of view, as well as the lack of molecular studies investigating the relationship between the mentioned thrips, we decided to perform analyses of both mitochondrial (COI) and nuclear markers (28S and ITS2) to determine the genetic diversity of H. leucanthemi and H. niger . Additionally, as a part of an integrative approach, we determined and analyzed their microbiota profiles, based on high-throughput 16S rRNA gene sequencing. The results of the molecular analyses revealed high intraspecific diversity of H. leucanthemi and did not support the distinctiveness of H. niger . The identified microbiota profiles were similar in both species and the performed analyses also did not support the distinctiveness of H. niger . Significant differences were, in turn, observed between H. leucanthemi and H. niger larvae. Moreover, two known endosymbiotic bacteria were found in the analyzed microbiota profiles (i.e., Wolbachia and Rickettsia ). Nevertheless, these symbionts were not predominantly found in the bacterial communities that are associated with H. niger and thus, its impact on the parthenogenetic mode of its reproduction seems less likely.

Lubricating oils are composed of base oils (>85% v/v) and enriching additives (<15% v/v). Three types of base oils may be distinguished: 1) traditional bases (obtained by low-volatile fractions from crude oil distillation refining), 2) synthetic bases (mainly poly-alpha-olefins, sometimes esters, especially succinic acid esters), 3) bases of natural origin (especially obtained from refined plant oils). The bases of natural origin are the only ones recommended for application when lubricating oil may be emitted to the environment (e.g. when the machine with an open cutting system is used). Group-type separation and analysis of group-type composition of base and lubricating oils are of significant importance in quality control and environmental monitoring. Due to the potentially wide range of polarity of the components of base and lubricating oils, group- type separation becomes a difficult separation problem. It is also a serious analytical problem due to the considerable diversity of physicochemical properties. The authors propose a new procedure for the separation and determination of the group-type composition of base and lubricating oils using thin-layer liquid chromatography in normal phase systems (abr. NP-TLC) on silica gel plates impregnated with berberine salt/in the coupling of thin-layer chromatography with flame ionization detection (abr. TLC-FID). A new, effective procedure of TLC plates impregnation with berberine sulphate was presented. The proposed procedure ensures the visualization of all groups of base oils. Extensive experimental research showed that a 2-step development procedure with application of n-hexane up to 100% height of development +15 min and further n-hexane: isopropanol: tri-fluoroacetic acid 96.25: 3: 0.75 (v: v: v) up to 75% height of development is advantageous for the group-type separation, both in TLC-FID and TLC., (Copyright © 2022 Elsevier B.V. All rights reserved.)

A review of the literature on emotion regulation in binge eating disorder (BED) published both in English and Polish between 1990 and 2020. BED might be considered as an impulsive and compulsive disorder associated with altered reward sensitivity and food-related attentional bias. The growing body of research indicated that there were corticostriatal circuitry alterations in BED, comparable to those observed in substance abuse, including altered function of orbitofrontal, prefrontal and insular cortices with the striatum included. Negative emotions and deficits in their regulation play a significant role in BED. Processing of anger, anxiety and sadness appear to be particularly important in this disorder. Research results identified an increase in negative emotions preceding episodes of binge eating. However, there is still inconsistency when it comes to whether these episodes alleviate negative affect. Individuals with BED more often use non-adaptive emotion regulation strategies, such as rumination and suppression of negative sensations. Whereas adaptive ones, for instance, cognitive reappraisal, are used less often. Clinical implications, besides pharmacology, highlight the high effectiveness of enhanced cognitive behavioral therapy (CBT-E), dialectic-behavioral therapy (DBT) and psychodynamic therapy in the treatment of emotional dysregulation in BED. Further studies, including ecological momentary assessment (EMA), should focus on emotional changes related to the binge cycle and the identification of reinforcing factors of BED.

The methods for hydrogen yield efficiency improvements, the gaseous stream purification in gaseous biofuels generation, and the biomass pretreatment are considered as the main trends in research devoted to gaseous biofuel production. The environmental aspect related to the liquid stream purification arises. Moreover, the management of post-fermentation broth with the application of various biorefining techniques gains importance. Chemical compounds occurring in the exhausted liquid phase after biomass pretreatment and subsequent dark and photo fermentation processes are considered as value-added by products. The most valuable are furfural (FF), 5-hydroxymethylfurfural (HMF), and levulinic acid (LA). Enriching their solutions can be carried with the application of liquid-liquid extraction with the use of a suitable solvent. In these studies, hydrophobic deep eutectic solvents (DESs) were tested as extractants. The screening of 56 DESs was carried out using the Conductor-like Screening Model for Real Solvents (COSMO-RS). DESs which exposed the highest inhibitory effect on fermentation and negligible water solubility were prepared. The LA, FF, and HMF were analyzed using FT-IR and NMR spectroscopy. In addition, the basic physicochemical properties of DES were carefully studied. In the second part of the paper, deep eutectic solvents were used for the extraction of FF, LA, and HMF from post-fermentation broth (PFB). The main extraction parameters, i.e., temperature, pH, and DES: PFB volume ratio (V DES :V PFB ), were optimized by means of a Box-Behnken design model. Two approaches have been proposed for extraction process. In the first approach, DES was used as a solvent. In the second, one of the DES components was added to the sample, and DES was generated in situ. To enhance the post-fermentation broth management, optimization of the parameters promoting HMF, FF, and LA extraction was carried under real conditions. Moreover, the antimicrobial effect of the extraction of FF, HMF, and LA was investigated to define the possibility of simultaneous separation of microbial parts and denatured peptides via precipitation.

Quantitative description of biochemical processes inside living cells and at single-molecule levels remains a challenge at the forefront of modern instrumentation and spectroscopy. This paper demonstrates such single-cell, single-molecule analyses performed to study the mechanism of action of olaparib - an up-to-date, FDA-approved drug for germline-BRCA mutated metastatic breast cancer. We characterized complexes formed with PARPi-FL - fluorescent analog of olaparib in vitro and in cancer cells using the advanced fluorescent-based method: Fluorescence Correlation Spectroscopy (FCS) combined with a length-scale dependent cytoplasmic/nucleoplasmic viscosity model. We determined in vitro olaparib-PARP1 equilibrium constant (6.06 × 10 8 mol L -1 ). In the cell nucleus, we distinguished three states of olaparib: freely diffusing drug (24%), olaparib-PARP1 complex (50%), and olaparib-PARP1-RNA complex (26%). We show olaparib accumulation in 3D spheroids, where intracellular concentration is twofold higher than in 2D cells. Moreover, olaparib concentration was tenfold higher (506 nmol L -1 vs. 57 nmol L -1 ) in cervical cancer (BRCA1 high abundance) than in breast cancer cells (BRCA1 low abundance) but with a lower toxic effect. Thus we confirmed that the amount of BRCA1 protein in the cells is a better predictor of the therapeutic effect of olaparib than its penetration into cancer tissue. Our single-molecule and single-cell approach give a new perspective of drug action in living cells. FCS provides a detailed in vivo insight, valuable in drug development and targeting.

Objective: The possible role of abnormal disgust processing in the development and course of anorexia nervosa (AN) has been emphasized in theoretical models and research. However, disgust toward external stimuli and self-disgust have not yet been investigated together in a clinical sample of AN patients. Therefore, the purpose of the study was to measure these constructs and examine their role in shaping eating pathology in AN patients and healthy controls (HCs), considering comorbid depressive and anxiety psychopathology. The study also aimed at testing the possible mediational roles of both disgust types in the associations between comorbid psychopathology and eating disorders (EDs) characteristics., Method: Altogether, 63 inpatients with AN and 57 HCs partook in the study. Participants completed the Eating Disorder Inventory-3, State-Trait Anxiety Inventory, Beck Depression Inventory-II, Disgust Scale-Revised and Self-Disgust Scale., Results: AN patients manifested higher self-disgust and disgust sensitivity than HCs. In addition, self-disgust predicted the severity of EDs characteristics and mediated the links of depressive symptoms and trait anxiety with EDs characteristics in both groups., Discussion: Our findings imply the putative role of self-disgust in the development of EDs psychopathology in HCs and in its maintenance in AN patients., (© 2021 Eating Disorders Association and John Wiley & Sons Ltd.)

Background: Inefficient mechanisms of emotional regulation appear essential in understanding the development and maintenance of binge-eating disorder (BED). Previous research focused mainly on a very limited emotion regulation strategies in BED, such as rumination, suppression, and positive reappraisal. Therefore, the aim of the study was to assess a wider range of emotional regulation strategies (i.e. acceptance, refocusing on planning, positive refocusing, positive reappraisal, putting into perspective, self-blame, other-blame, rumination, and catastrophizing), as well as associations between those strategies and binge-eating-related beliefs (negative, positive, and permissive), and clinical variables (eating disorders symptoms, both anxiety, depressive symptoms, and alexithymia)., Methods: Women diagnosed with BED (n = 35) according to the DSM-5 criteria and healthy women (n = 41) aged 22-60 years were assessed using: the Eating Attitudes Test-26, the Eating Beliefs Questionnaire-18, the Hospital Anxiety and Depression Scale, the Toronto Alexithymia Scale-20, the Cognitive Emotion Regulation Questionnaire, and the Difficulties in Emotion Regulation Scale. Statistical analyses included: Student t - tests or Mann-Whitney U tests for testing group differences between BED and HC group, and Pearson's r coefficient or Spearman's rho for exploring associations between the emotion regulation difficulties and strategies, and clinical variables and binge-eating-related beliefs in both groups., Results: The BED group presented with a significantly higher level of emotion regulation difficulties such as: nonacceptance of emotional responses, lack of emotional clarity, difficulties engaging in goal-directed behavior, impulse control difficulties, and limited access to emotion regulation strategies compared to the healthy controls. Moreover, patients with BED were significantly more likely to use maladaptive strategies (rumination and self-blame) and less likely to use adaptive strategies (positive refocusing and putting into perspective). In the clinical group, various difficulties in emotion regulation difficulties occurred to be positively correlated with the level of alexithymia, and anxiety and depressive symptoms. Regarding emotion regulation strategies, self-blame and catastrophizing were positively related to anxiety symptoms, but solely catastrophizing was related to the severity of eating disorder psychopathology., Conclusions: Our results indicate an essential and still insufficiently understood role of emotional dysregulation in BED. An especially important construct in this context seems to be alexithymia, which was strongly related to the majority of emotion regulation difficulties. Therefore, it might be beneficial to pay special attention to this construct when planning therapeutic interventions, as well as to the maladaptive emotion regulation strategies self-blame and catastrophizing, which were significantly related to BED psychopathology., (© 2021. The Author(s).)

As an element of distorted self-image, body image disturbances may be relevant to borderline personality disorder (BPD). Therefore, this systematic review aims to critically discuss and summarize empirical findings in this matter. Based on the available theoretical models, three body image components were identified: (a) perception, (b) affect and cognition, and (c) general body dissatisfaction. We conducted a systematic search of the empirical literature published in English in the MEDLINE, PsycINFO, and Scopus databases until June 2021 using a priori eligibility criteria (BPD; BPD symptoms or features in nonclinical groups; quasipsychotic or psychotic symptoms were not considered). We included k = 10 records meeting the criteria. Compared with other analyzed groups, individuals diagnosed with BPD obtained higher scores in the three components of body image disturbances. The issue of body image in BPD is relatively understudied, although current research findings clearly indicate disturbances in all of the abovementioned body image components in individuals with BPD or significant relationships of these components with BPD traits or symptoms both in clinical and nonclinical samples. Eventually, possible practical implications and future research directions are also discussed.

The oxazole yellow dye, YOYO-1 (a symmetric homodimer), is a commonly used molecule for staining DNA. We applied the brightness analysis to study the intercalation of YOYO-1 into the DNA. We distinguished two binding modes of the dye to dsDNA: mono-intercalation and bis-intercalation. Bis-intercalation consists of two consecutive mono-intercalation steps, characterised by two distinct equilibrium constants (with the average number of base pair per binding site equals 3.5): K1=3.36±0.43×107M-1 and K2=1.90±0.61×105M-1, respectively. Mono-intercalation dominates at high concentrations of YOYO-1. Bis-intercalation occurs at low concentrations.

Piscivorous avian species may be affected by mercury (Hg) which tends to accumulate in aquatic environments and biomagnifies across the food webs. One of such species is the black stork, whose population increase recently slowed down due to unknown reasons. At the same time Hg contamination and its effects were almost unaudited for this species, so it may have exerted deleterious effects on the population and an evaluation is necessary. This is the first study of this species concerning Hg contamination. Thus, Hg concentrations were investigated in the down of black stork chicks (N = 90) from breeding locations in central and southern Poland (Europe) between 2015 and 2017. As well as Hg levels, morphometric parameters and age were evaluated. Mean Hg concentrations reached 0.7 μg/g d.w. and differed significantly between years, from the lowest value noted in 2017 (mean 0.5 μg/g), through 2016 (0.7 μg/g), to the highest one in 2015 (0.9 μg/g), and between nest locations where higher Hg levels were generally found in northern parts of the study area. Hg concentrations were also unrelated to morphometric parameters. Contrarily, morphometric parameters revealed high correlations between themselves, which was confirmed by the cluster analysis (revealing only two clusters) and principal component analysis (the first PC explained 96.8% of the variance). Hg levels in the down of black storks were rather low with the fluctuation between years and nest locations probably caused by parental exposure during wintering, migration, pre-breeding season and recent exposure through food provided by parents. Such low Hg concentrations seemed not to affect the population from the region studied., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Ciliopathies are inherited disorders caused by defects in motile and non-motile (primary) cilia. Ciliopathy syndromes and associated gene variants are often highly pleiotropic and represent exemplars for interrogating genotype-phenotype correlations. Towards understanding disease mechanisms in the context of ciliopathy mutations, we have used a leading model organism for cilia and ciliopathy research, Caenorhabditis elegans, together with gene editing, to characterise two missense variants (P74S and G155S) in mksr-2/B9D2 associated with Joubert syndrome (JBTS). B9D2 functions within the Meckel syndrome (MKS) module at the ciliary base transition zone (TZ) compartment and regulates the molecular composition and sensory/signalling functions of the cilium. Quantitative assays of cilium/TZ structure and function, together with knock-in reporters, confirm that both variant alleles are pathogenic in worms. G155S causes a more severe overall phenotype and disrupts endogenous MKSR-2 organisation at the TZ. Recapitulation of the patient biallelic genotype shows that compound heterozygous worms phenocopy worms homozygous for P74S. The P74S and G155S alleles also reveal evidence of a very close functional association between the B9D2-associated B9 complex and MKS-2/TMEM216. Together, these data establish C. elegans as a model for interpreting JBTS mutations and provide further insight into MKS module organisation. This article has an associated First Person interview with the first author of the paper., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2021. Published by The Company of Biologists Ltd.)

Background: The pH of the skin surface is usually between 5.4 and 5.9 and functions as a barrier against bacteria and fungi; thus, the composition of the topically applied drug form may be of high importance for proper medication., Objectives: To evaluate the influence of the measurement conditions in aqueous solutions of ointments, creams, and gels, which include polymeric components, on the pH and conductivity results., Material and Methods: The pH and electrolytic conductivity of aqueous dispersions of commercially available ointments, creams and gels were tested and compared to reference vehicles., Results: The results of the dilution method measurements of the pH and electrolytic conductivity of the ointment preparations are highly diverse, ranging from 5.88 to 6.27, whereas the reference pH for Unguentum simplex was between 5.40 and 5.43. Furthermore, the measurements of the pH and electrolytic conductivity with the dilution method for creams did not provide repeatable results with a small sample size, and the pH of commercial preparations was in the range between 5.79 and 6.37, compared to the reference pH of 5.23-5.46. However, the dilution method for measurements of the pH and electrolytic conductivity was suitable for hydrogel preparations and the obtained results were repeatable in the range of 6.11-6.90, while the reference preparations were in the range of 5.19-5.62., Conclusions: Evaluation methods of the electrolytic conductivity and pH of the preparations applied on the skin should be further evaluated; however, the pH of the commercial preparation seems to differ from the physiological skin pH, which covers the range of reference preparations.

Background and Aims: Binge eating disorder (BED) is correlated with substance use. This study aimed to estimate the life-time prevalence of alcohol use disorder (AUD) among individuals with non-compensatory binge eating and determine whether their life-time prevalence of AUD is higher than in non-bingeing controls., Design: A systematic search of databases (PubMed, Embase and Web of Science) for studies of adults diagnosed with BED or a related behavior that also reported the life-time prevalence of AUD was conducted. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol was followed. The protocol was registered on the International Prospective Register of Systematic Reviews (PROSPERO)., Setting: Studies originating in Canada, Sweden, the United Kingdom and the United States., Participants: Eighteen studies meeting the inclusion criteria were found, representing 69 233 individuals., Measurements: Life-time prevalence of AUD among individuals with binge eating disorder and their life-time relative risk of AUD compared with individuals without this disorder., Results: The pooled life-time prevalence of AUD in individuals with binge eating disorder was 19.9% [95% confidence interval (CI) = 13.7-27.9]. The risk of life-time AUD incidence among individuals with binge eating disorder was more than 1.5 times higher than controls (relative risk = 1.59, 95% CI = 1.41-1.79). Life-time AUD prevalence was higher in community samples than in clinical samples (27.45 versus 14.45%, P = 0.041) and in studies with a lower proportion of women (β = -2.2773, P = 0.044)., Conclusions: Life-time alcohol use disorder appears to be more prevalent with binge eating disorder than among those without., (© 2020 Society for the Study of Addiction.)

Objectives: The aim of this publication is to analyze the organizational units of mental healthcare for adults based on the data on the services reported to the National Health Fund in 2010-2016., Methods: The following organizational forms of care were analyzed: mental health outpatient clinics, general psychiatric wards, general day care psychiatric wards, community mental health teams, and psychiatric emergency rooms. These organizational units were analyzed in terms of their number, utilization and accessibility. In addition, a preliminary simulation of the expected Mental Health Centers was carried out., Results: In Poland, in 2010-2016, the number of mental health service providers under contracts with the National Health Fund increased by 5%. The most robust growth was observed for community mental health teams, whose number increased by 282%. However, this organizational form was used by a marginal (1.9%) percentage of patients. The highest rate of admission to general psychiatric wards was observed in districts where a general psychiatric ward and a mental health clinic were available with no day care psychiatric wards or community mental health teams. Asmall number of entities providing comprehensive care was in operation in 2016. The preliminary simulation has shown that in 2016 a total of 156 MentalHealth Centers should have been in operation, assuming that each of them would have provided care for 200,000 inhabitants., Conclusions: It would be advisable to analyze the exact geographic distribution of units, human resources in individual organizational units, and to take financial outlays for mental healthcare in their various forms into consideration.

Objectives: In Poland, there is no systematic epidemiological research on mental disorders of children and adolescents in the general population, as well as a register dedicated to mental disorders. The aim of the presented study is to analyze psychiatric services reported to the National Health Fund that were provided to children and adolescents in the years 2010-2016., Methods: The starting point of this study was report entitled Maps of health needs in mental disorders published by the Ministry of Health. The analysis concerns all mental health services provided to children and adolescents in the years 2010-2016. The analysis covers seven largest, in terms of the number of patients, groups of mental disorders according to ICD-10 in children and adolescents., Results: In the years 2010-2016, both the number of psychiatric service users under the age of 18 years and the total number of healthcare services have increased in almost all of analyzed areas. Unusually high increase in the number of services provided in the ad hoc mode in A&E department was registered, with a very small share of services provided in home environment. In almost all analyzed groups of mental disorders, there was a greater number of boys, as well as residents of a town/city. Only in the group of neurotic disorders associated with stress and in the somatic form a slightly higher number of girls was noticed., Conclusions: In the years 2010-2016, an increase in the number of registered cases in the population of children and adolescents and the number of services in almost all of the analyzed areas was observed. High increase in services provided in the ad hoc mode in A&E department and a small share of services provided in the home environment indicates significant discrepancy between the needs and the availability of resources.

Background: Yeasts, which are ubiquitous in agroecosystems, are known to degrade various xenobiotics. The aim of this study was to analyze the effect of fungicides on the abundance of natural yeast communities colonizing winter wheat leaves, to evaluate the sensitivity of yeast isolates to fungicides in vivo, and to select yeasts that degrade propiconazole., Results: Fungicides applied during the growing season generally did not affect the counts of endophytic yeasts colonizing wheat leaves. Propiconazole and a commercial mixture of flusilazole and carbendazim decreased the counts of epiphytic yeasts, but the size of the yeast community was restored after 10 days. Epoxiconazole and a commercial mixture of fluoxastrobin and prothioconazole clearly stimulated epiphyte growth. The predominant species isolated from leaves were Aureobasidium pullulans and Rhodotorula glutinis. In the disk diffusion test, 14 out of 75 yeast isolates were not sensitive to any of the tested fungicides. After 48 h of incubation in an aqueous solution of propiconazole, the Rhodotorula glutinis Rg 55 isolate degraded the fungicide in 75%. Isolates Rh. glutinis Rg 92 and Rg 55 minimized the phytotoxic effects of propiconazole under greenhouse conditions. The first isolate contributed to an increase in the dry matter content of wheat seedlings, whereas the other reduced the severity of chlorosis., Conclusion: Not sensitivity of many yeast colonizing wheat leaves on the fungicides and the potential of isolate Rhodotorula glutinis Rg 55 to degrade of propiconazole was established. Yeast may partially eliminate the ecologically negative effect of fungicides.

Objective: A growing body of empirical literature indicates altered taste perception in individuals with anorexia nervosa (AN). However, it remains unknown whether the observed impairments in the neural processing of taste stimuli represent etiopathogenetic factors of AN or whether they are a secondary consequence of malnutrition., Method: In the current systematic review, scientific studies were identified using the MEDLINE, PsycINFO, and Scopus databases. A supplemental search was performed by searches through reference lists of the relevant publications and via Google Scholar., Results: On the basis of the searches conducted, 16 publications were identified and included in this literature review. The results of those studies point to disturbances in the structure and functioning of brain regions involved in taste processing in AN., Conclusions: The findings of the reviewed studies suggest that altered reward, interoceptive, and cognitive-emotional processing may contribute to abnormal taste processing in AN., (© 2019 John Wiley & Sons, Ltd and Eating Disorders Association.)

Objectives: The aim of the study was the analysis of the occurrence of cardiovascular risk factors (age, sex, smoking, level of cholesterol, systolic blood pressure, body mass index) among patients diagnosed with schizophrenia as well as searching for correlations between them and length of stay at long-term care facilities and clinical variables, such as severity of positive, negative and general symptoms of schizophrenia, illness duration, and type of pharmacotherapy (neuroleptic type and its dose)., Methods: Medical data were collected from 71 patients (30 women and 41 men) aged between 40 and 86 years who were treated in the residential care facility due to paranoid schizophrenia. Information concerning patient's clinical status was collected during periodic check-ups. Theexamination consisted of standard anamnesis regarding patient's general feeling, psychiatric assessment using the PANSS and identifying number of smoked cigarettes. Somatic assessment included: physical examination, measuring blood pressure, pulse, capillary blood glucose levels, height, weight, and BMI., Results: Statistical analysis did not reveal significant differences in length of stay at long-term psychiatric care facility between patients with normal BMI vs. overweight and obese patients (p = 0.85). Study results indicate that prevalence of central obesity, hypertension, abnormal total - and LDL-cholesterol is higher in patients who stay at the long-term psychiatric care facility for a longer period of time. There is at least one cardiovascular risk factor in 74.6% of examined patients treated for schizophrenia., Conclusions: Imposing "healthy" lifestyle as part of clinical management in long-termcare setting in people treated with neuroleptics (atypical or typical) might reduce risk of body mass increase. Undertaking action towards reducing modifiable cardiovascular risk factors remains highly recommended in mental health care, particularly in long-term care.

Objectives: Analyzing the indices that allow to improve population health by, for example, improving the quality of healthcare services and increasing accessibility to these services is among the priorities of the World Health Organization (WHO). This is of particular importance in Poland, as the psychiatric care reform is being carried out in accordance with the National Mental Healthcare Program guidelines. The aim of the study is to analyze mental health services provided to adults and reported to the National Health Fund in 2010-2014., Methods: In the present study, we expanded the information included in the second part of the maps of health needs. In addition to the evaluation of services provided to adults in 2014, we presented an analysis of services in 2010-2014. According to these data, there was a continuous increase both in thenumber of individuals provided with services for psychiatric disorders and in the total number of provided services. There was an increase in the number of patients treated for mood disorders, neurotic disorders, stress-related and somatic disorders, as well as addictions., Results: The increase in the total number of services was mainly seen for outpatient types of care. The reasons why in 2014 there was a resurgence in psychiatric hospitalizations and in the hospitalization rate per 100 thousand adults remains unclear., Conclusions: Our results indicate the need for further support of the development of psychiatric care using multidirectional efforts within an integrated model for solving health problems. An overall analysis of services provided in psychiatric care requires access to information on services funded from non-public sources and expanding the reported information.

Objective: The empirical literature describes the role of the oxytocinergic system in emotion perception (EP). Variants in the oxytocin (OXT) and oxytocin receptor genes have been associated with mental disorders, including anorexia nervosa (AN), that are characterized by difficulties in socioemotional functioning. Our study aimed to examine whether variability within the genes related to OXT pathways may play a role in facial EP in inpatients with AN., Method: Single nucleotide polymorphisms (SNPs) of the following genes: oxytocin receptor (rs2254298, rs53576), OXT (rs6133010), OXT-arginine-vasopressin (rs2740204), CD38 (rs6449197, rs3796863), and human leucyl/cystinylaminopeptidase (rs4869317) were genotyped in 60 AN female inpatients and 60 healthy control females (HCs). Associations between genetic polymorphisms and EP as well as clinical symptoms were examined., Results: The AN group showed decreased EP abilities compared with HCs. SNPs of rs2740204, rs6133010, and rs53576 were associated with differences in EP in women with AN and in HCs. The SNP of rs4869317 was associated with the level of eating disorders symptoms in HCs., Conclusions: The OXT system may be involved in EP difficulties in AN. SNPs within genes related to OXT pathways may influence EP abilities. The leucyl/cystinylaminopeptidase rs4869317 SNP may be involved in the development of eating disorders psychopathology., (© 2019 John Wiley & Sons, Ltd and Eating Disorders Association.)

Concentrations of mercury (Hg) were examined in the blood of mute swans from rural breeding sites and urban wintering areas in southern parts of Poland, Europe. The birds were classified into three age groups: cygnets, juveniles and adults. To investigate the potential impact of Hg on birds, hematocrit (Ht), reduced glutathione (GSH) levels and morphometric measurements were taken. Using morphometric parameters, we stated that all mute swans sampled were in good condition. The mercury concentrations found were rather low and differed between birds from industrialized wintering areas and rural breeding areas (means 7 ng/mL and 2 ng/mL, respectively). We found no difference in Hg concentrations between the sexes, but concentrations varied significantly between age groups (cygnets 2 ng/mL, juveniles 7 ng/mL and adults 6 ng/mL). A similar trend was observed for hematocrit levels. GSH levels did not differ between any of the groups studied. We found no significant relationship between blood parameters (Ht, GSH) in relation to Hg concentrations. We conclude that the Hg concentrations in blood may be influenced by industrialization, season and age, but generally low concentration such as those found by us do not affect Ht and GSH levels., (Copyright © 2019 Elsevier Ltd. All rights reserved.)