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20 results on '"Krutzke, Lea"'

4. Insights in ChAdOx1 nCoV-19 vaccine-induced immune thrombotic thrombocytopenia

Author

Greinacher, Andreas, Selleng, Kathleen, Palankar, Raghavendra, Wesche, Jan, Handtke, Stefan, Wolff, Martina, Aurich, Konstanze, Lalk, Michael, Methling, Karen, Völker, Uwe, Hentschker, Christian, Michalik, Stephan, Steil, Leif, Reder, Alexander, Schönborn, Linda, Beer, Martin, Franzke, Kati, Büttner, Andreas, Fehse, Boris, Stavrou, Evi X., Rangaswamy, Chandini, Mailer, Reiner K., Englert, Hanna, Frye, Maike, Thiele, Thomas, Kochanek, Stefan, Krutzke, Lea, Siegerist, Florian, Endlich, Nicole, Warkentin, Theodore E., and Renné, Thomas

Published
2021
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5. Heterologous DNA-prime/protein-boost immunization with a monomeric SARS-CoV-2 spike antigen redundantizes the trimeric receptor-binding domain structure to induce neutralizing antibodies in old mice

Author

Pflumm, Dominik, primary, Seidel, Alina, additional, Klein, Fabrice, additional, Groß, Rüdiger, additional, Krutzke, Lea, additional, Kochanek, Stefan, additional, Kroschel, Joris, additional, Münch, Jan, additional, Stifter, Katja, additional, and Schirmbeck, Reinhold, additional

Published
2023
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7. Human Monocytes Are Suitable Carriers for the Delivery of Oncolytic Herpes Simplex Virus Type 1 In Vitro and in a Chicken Embryo Chorioallantoic Membrane Model of Cancer

Author

Reale, A, Krutzke, L, Cadamuro, M, Vitiello, A, von Einem, J, Kochanek, S, Palù, G, Parolin, C, Calistri, A, Reale, Alberto, Krutzke, Lea, Cadamuro, Massimiliano, Vitiello, Adriana, von Einem, Jens, Kochanek, Stefan, Palù, Giorgio, Parolin, Cristina, Calistri, Arianna, Reale, A, Krutzke, L, Cadamuro, M, Vitiello, A, von Einem, J, Kochanek, S, Palù, G, Parolin, C, Calistri, A, Reale, Alberto, Krutzke, Lea, Cadamuro, Massimiliano, Vitiello, Adriana, von Einem, Jens, Kochanek, Stefan, Palù, Giorgio, Parolin, Cristina, and Calistri, Arianna

Abstract

Oncolytic viruses (OVs) are promising therapeutics for tumors with a poor prognosis. An OV based on herpes simplex virus type 1 (oHSV-1), talimogene laherparepvec (T-VEC), has been recently approved by the Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the treatment of unresectable melanoma. T-VEC, like most OVs, is administered via intratumoral injection, underlining the unresolved problem of the systemic delivery of the oncolytic agent for the treatment of metastases and deep-seated tumors. To address this drawback, cells with a tropism for tumors can be loaded ex vivo with OVs and used as carriers for systemic oncolytic virotherapy. Here, we evaluated human monocytes as carrier cells for a prototype oHSV-1 with a similar genetic backbone as T-VEC. Many tumors specifically recruit monocytes from the bloodstream, and autologous monocytes can be obtained from peripheral blood. We demonstrate here that oHSV-1-loaded primary human monocytes migrated in vitro towards epithelial cancer cells of different origin. Moreover, human monocytic leukemia cells selectively delivered oHSV-1 to human head-and-neck xenograft tumors grown on the chorioallantoic membrane (CAM) of fertilized chicken eggs after intravascular injection. Thus, our work shows that monocytes are promising carriers for the delivery of oHSV-1s in vivo, deserving further investigation in animal models.

Published
2023

11. Vaccine-induced COVID-19 mimicry syndrome

Author

Kowarz, Eric, primary, Krutzke, Lea, primary, Külp, Marius, additional, Streb, Patrick, additional, Larghero, Patrizia, additional, Reis, Jennifer, additional, Bracharz, Silvia, additional, Engler, Tatjana, additional, Kochanek, Stefan, additional, and Marschalek, Rolf, additional

Published
2022
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12. Author response: Vaccine-induced COVID-19 mimicry syndrome

Author

Kowarz, Eric, primary, Krutzke, Lea, primary, Külp, Marius, additional, Streb, Patrick, additional, Larghero, Patrizia, additional, Reis, Jennifer, additional, Bracharz, Silvia, additional, Engler, Tatjana, additional, Kochanek, Stefan, additional, and Marschalek, Rolf, additional

Published
2022
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13. Virotherapy in Germany—Recent Activities in Virus Engineering, Preclinical Development, and Clinical Studies

Author

Nettelbeck, Dirk M., primary, Leber, Mathias F., additional, Altomonte, Jennifer, additional, Angelova, Assia, additional, Beil, Julia, additional, Berchtold, Susanne, additional, Delic, Maike, additional, Eberle, Jürgen, additional, Ehrhardt, Anja, additional, Engeland, Christine E., additional, Fechner, Henry, additional, Geletneky, Karsten, additional, Goepfert, Katrin, additional, Holm, Per Sonne, additional, Kochanek, Stefan, additional, Kreppel, Florian, additional, Krutzke, Lea, additional, Kühnel, Florian, additional, Lang, Karl Sebastian, additional, Marchini, Antonio, additional, Moehler, Markus, additional, Mühlebach, Michael D., additional, Naumann, Ulrike, additional, Nawroth, Roman, additional, Nüesch, Jürg, additional, Rommelaere, Jean, additional, Lauer, Ulrich M., additional, and Ungerechts, Guy, additional

Published
2021
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14. Transduction Enhancers Enable Efficient Human Adenovirus Type 5-Mediated Gene Transfer into Human Multipotent Mesenchymal Stromal Cells

Author

Nilson, Robin, primary, Lübbers, Olivia, additional, Weiß, Linus, additional, Singh, Karmveer, additional, Scharffetter-Kochanek, Karin, additional, Rojewski, Markus, additional, Schrezenmeier, Hubert, additional, Zeplin, Philip Helge, additional, Funk, Wolfgang, additional, Krutzke, Lea, additional, Kochanek, Stefan, additional, and Kritzinger, Astrid, additional

Published
2021
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16. EGFR-Binding Peptides: From Computational Design towards Tumor-Targeting of Adeno-Associated Virus Capsids

Author

Feiner, Rebecca, Kemker, Isabell, Krutzke, Lea, Allmendinger, Ellen, Mandell, Daniel J., Sewald, Norbert, Kochanek, Stefan, and Müller, Kristian

Subjects
viruses, Genetic Vectors, Transplantation, Heterologous, Chick Embryo, Synthetische Biologie, Chorioallantoic Membrane, Article, Cyclopeptide, cyclic peptide, lcsh:Chemistry, DDC 570 / Life sciences, Capsid, ddc:570, Cell Line, Tumor, Proteindesign, Animals, Humans, ddc:610, Cyclic peptides, lcsh:QH301-705.5, Synthetic biology, oncolytic virus, Wound Healing, VP protein, Circular Dichroism, Computational Biology, protein engineering, Genetic Therapy, Dependovirus, Up-Regulation, ErbB Receptors, Oncolytic Viruses, Microscopy, Fluorescence, lcsh:Biology (General), lcsh:QD1-999, VDEPT, Capsid Proteins, Protein engineering, synthetic biology, Peptides, DDC 610 / Medicine & health, Dimerization, Protein Binding
Abstract

The epidermal growth factor receptor (EGFR) plays a central role in the progression of many solid tumors. We used this validated target to analyze the de novo design of EGFR-binding peptides and their application for the delivery of complex payloads via rational design of a viral vector. Peptides were computationally designed to interact with the EGFR dimerization interface. Two new peptides and a reference (EDA peptide) were chemically synthesized, and their binding ability characterized. Presentation of these peptides in each of the 60 capsid proteins of recombinant adeno-associated viruses (rAAV) via a genetic based loop insertion enabled targeting of EGFR overexpressing tumor cell lines. Furthermore, tissue distribution and tumor xenograft specificity were analyzed with systemic injection in chicken egg chorioallantoic membrane (CAM) assays. Complex correlations between the targeting of the synthetic peptides and the viral vectors to cells and in ovo were observed. Overall, these data demonstrate the potential of computational design in combination with rational capsid modification for viral vector targeting opening new avenues for viral vector delivery and specifically suicide gene therapy., publishedVersion

Published
2020

17. Towards Understanding ChAdOx1 nCov-19 Vaccine-induced Immune Thrombotic Thrombocytopenia (VITT)

Author

Greinacher, Andreas, primary, Selleng, Kathleen, additional, Wesche, Jan, additional, Handtke, Stefan, additional, Palankar, Raghavendra, additional, Aurich, Konstanze, additional, Lalk, Michael, additional, Methling, Karen, additional, Völker, Uwe, additional, Hentschker, Christian, additional, Michalik, Stephan, additional, Steil, Leif, additional, Schönborn, Linda, additional, Beer, Martin, additional, Franzke, Kati, additional, Rangaswamy, Chandini, additional, Mailer, Reiner K., additional, Thiele, Thomas, additional, Kochanek, Stefan, additional, Krutzke, Lea, additional, Siegerist, Florian, additional, Endlich, Nicole, additional, Warkentin, Theodore E., additional, and Renné, Thomas, additional

Published
2021
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