1,271 results on '"Kronenberg, Mitchell"'
Search Results
2. A regulatory circuit controlled by extranuclear and nuclear retinoic acid receptor α determines T cell activation and function.
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Larange, Alexandre, Takazawa, Ikuo, Kakugawa, Kiyokazu, Thiault, Nicolas, Ngoi, SooMun, Olive, Meagan, Iwaya, Hitoshi, Seguin, Laetitia, Vicente-Suarez, Ildefonso, Becart, Stephane, Verstichel, Greet, Balancio, Ann, Altman, Amnon, Chang, John, Taniuchi, Ichiro, Lillemeier, Bjorn, Kronenberg, Mitchell, Myers, Samuel, and Cheroutre, Hilde
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FOXP3(+) regulatory T cells ,T cell receptor ,ZAP-70 ,anti-pathogen immunity ,autoimmune disease ,cellular-retinoic-acid-binding protein ,effector differentiation ,extranuclear ,nuclear receptor ,proliferation ,retinoic acid ,retinoic acid receptor alpha ,signal transduction ,Humans ,Retinoic Acid Receptor alpha ,Lymphocyte Activation ,Autoimmune Diseases ,Cell Membrane ,Receptors ,Antigen ,T-Cell - Abstract
Ligation of retinoic acid receptor alpha (RARα) by RA promotes varied transcriptional programs associated with immune activation and tolerance, but genetic deletion approaches suggest the impact of RARα on TCR signaling. Here, we examined whether RARα would exert roles beyond transcriptional regulation. Specific deletion of the nuclear isoform of RARα revealed an RARα isoform in the cytoplasm of T cells. Extranuclear RARα was rapidly phosphorylated upon TCR stimulation and recruited to the TCR signalosome. RA interfered with extranuclear RARα signaling, causing suboptimal TCR activation while enhancing FOXP3+ regulatory T cell conversion. TCR activation induced the expression of CRABP2, which translocates RA to the nucleus. Deletion of Crabp2 led to increased RA in the cytoplasm and interfered with signalosome-RARα, resulting in impaired anti-pathogen immunity and suppressed autoimmune disease. Our findings underscore the significance of subcellular RA/RARα signaling in T cells and identify extranuclear RARα as a component of the TCR signalosome and a determinant of immune responses.
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- 2023
3. Intravital Imaging of Intestinal Intraepithelial Lymphocytes.
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McArdle, Sara, Seo, Goo-Young, Mikulski, Zbigniew, and Kronenberg, Mitchell
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Cell labeling ,Confocal reflection ,Intestine ,Intraepithelial lymphocyte ,Intravital microscopy ,Suction ring ,Tracking - Abstract
Intestinal intraepithelial lymphocytes (IEL) are a numerous population of T cells located within the epithelium of the small and large intestines, being more numerous in the small intestine (SI). They surveil this tissue by interacting with epithelial cells. Intravital microscopy is an important tool for visualizing the patrolling activity of IEL in the SI of live mice. Most IEL express CD8α; therefore, here we describe an established protocol of intravital imaging that tracks lymphocytes labeled with a CD8α-specific monoclonal antibody in the SI epithelium of live mice. We also describe data acquisition and quantification of the movement metrics, including mean speed, track length, displacement length, and paths for each CD8α+ IEL using the available software. The intravital imaging technique for measuring IEL movement will provide a better understanding of the role of IEL in homeostasis and protection from injury or infection in vivo.
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- 2023
4. Divergent metabolic programmes control two populations of MAIT cells that protect the lung.
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Riffelmacher, Thomas, Paynich Murray, Mallory, Wientjens, Chantal, Chandra, Shilpi, Cedillo-Castelán, Viankail, Chou, Ting-Fang, McArdle, Sara, Dillingham, Christopher, Devereaux, Jordan, Nilsen, Aaron, Brunel, Simon, Lewinsohn, David, Hasty, Jeff, Seumois, Gregory, Benedict, Christopher, Vijayanand, Pandurangan, and Kronenberg, Mitchell
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Mice ,Animals ,Mucosal-Associated Invariant T Cells ,Lung - Abstract
Although mucosal-associated invariant T (MAIT) cells provide rapid, innate-like responses, they are not pre-set, and memory-like responses have been described for MAIT cells following infections. The importance of metabolism for controlling these responses, however, is unknown. Here, following pulmonary immunization with a Salmonella vaccine strain, mouse MAIT cells expanded as separate CD127-Klrg1+ and CD127+Klrg1- antigen-adapted populations that differed in terms of their transcriptome, function and localization in lung tissue. These populations remained altered from steady state for months as stable, separate MAIT cell lineages with enhanced effector programmes and divergent metabolism. CD127+ MAIT cells engaged in an energetic, mitochondrial metabolic programme, which was critical for their maintenance and IL-17A synthesis. This programme was supported by high fatty acid uptake and mitochondrial oxidation and relied on highly polarized mitochondria and autophagy. After vaccination, CD127+ MAIT cells protected mice against Streptococcus pneumoniae infection. In contrast, Klrg1+ MAIT cells had dormant but ready-to-respond mitochondria and depended instead on Hif1a-driven glycolysis to survive and produce IFN-γ. They responded antigen independently and participated in protection from influenza virus. These metabolic dependencies may enable tuning of memory-like MAIT cell responses for vaccination and immunotherapies.
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- 2023
5. SARS-CoV-2 Omicron (B.1.1.529) shows minimal neurotropism in a double-humanized mouse model
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Alves, Rubens Prince Dos Santos, Wang, Ying-Ting, Mikulski, Zbigniew, McArdle, Sara, Shafee, Norazizah, Valentine, Kristen M, Miller, Robyn, Verma, Shailendra Kumar, Batiz, Fernanda Ana Sosa, Maule, Erin, Nguyen, Michael N, Timis, Julia, Mann, Colin, Zandonatti, Michelle, Alarcon, Suzie, Rowe, Jenny, Kronenberg, Mitchell, Weiskopf, Daniela, Sette, Alessandro, Hastie, Kathryn, Saphire, Erica Ollmann, Festin, Stephen, Kim, Kenneth, and Shresta, Sujan
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Biomedical and Clinical Sciences ,Immunology ,Emerging Infectious Diseases ,Lung ,Infectious Diseases ,Coronaviruses ,Biodefense ,2.1 Biological and endogenous factors ,Aetiology ,Infection ,Good Health and Well Being ,Animals ,Humans ,Mice ,SARS-CoV-2 ,COVID-19 ,Brain ,Antiviral Agents ,Disease Models ,Animal ,Omicron ,Neurotropism ,Mouse model ,Human ACE2 ,Human CD34 immune cells ,T cell ,NCG ,Microbiology ,Medical Microbiology ,Pharmacology and Pharmaceutical Sciences ,Virology ,Medical microbiology ,Pharmacology and pharmaceutical sciences - Abstract
Although severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) initially infects the respiratory tract, it also directly or indirectly affects other organs, including the brain. However, little is known about the relative neurotropism of SARS-CoV-2 variants of concern (VOCs), including Omicron (B.1.1.529), which emerged in November 2021 and has remained the dominant pathogenic lineage since then. To address this gap, we examined the relative ability of Omicron, Beta (B.1.351), and Delta (B.1.617.2) to infect the brain in the context of a functional human immune system by using human angiotensin-converting enzyme 2 (hACE2) knock-in triple-immunodeficient NGC mice with or without reconstitution with human CD34+ stem cells. Intranasal inoculation of huCD34+-hACE2-NCG mice with Beta and Delta resulted in productive infection of the nasal cavity, lungs, and brain on day 3 post-infection, but Omicron was surprisingly unique in its failure to infect either the nasal tissue or brain. Moreover, the same infection pattern was observed in hACE2-NCG mice, indicating that antiviral immunity was not responsible for the lack of Omicron neurotropism. In independent experiments, we demonstrate that nasal inoculation with Beta or with D614G, an ancestral SARS-CoV-2 with undetectable replication in huCD34+-hACE2-NCG mice, resulted in a robust response by human innate immune cells, T cells, and B cells, confirming that exposure to SARS-CoV-2, even without detectable infection, is sufficient to induce an antiviral immune response. Collectively, these results suggest that modeling of the neurologic and immunologic sequelae of SARS-CoV-2 infection requires careful selection of the appropriate SARS-CoV-2 strain in the context of a specific mouse model.
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- 2023
6. Amerindian ancestry proportion as a risk factor for inflammatory bowel diseases: results from a Latin American Andean cohort.
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Pérez-Jeldres, Tamara, Magne, Fabien, Ascui, Gabriel, Alvares, Danilo, Orellana, Matias, Alvarez-Lobos, Manuel, Hernandez-Rocha, Cristian, Azocar, Lorena, Aguilar, Nataly, Espino, Alberto, Estela, Ricardo, Escobar, Sergio, Zazueta, Alejandra, Baez, Pablo, Silva, Verónica, De La Vega, Andres, Arriagada, Elizabeth, Pavez-Ovalle, Carolina, Díaz-Asencio, Alejandro, Travisany, Dante, Miquel, Juan, Villablanca, Eduardo, Kronenberg, Mitchell, and Bustamante, María
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Latin American ,ancestry ,genetics ,inflammatory bowel disease ,single nucleotide polymorphism (SNP) - Abstract
BACKGROUND AND AIMS: Latin American populations remain underrepresented in genetic studies of inflammatory bowel diseases (IBDs). Most genetic association studies of IBD rely on Caucasian, African, and Asian individuals. These associations have yet to be evaluated in detail in the Andean region of South America. We explored the contribution of IBD-reported genetic risk variants to a Chilean cohort and the ancestry contribution to IBD in this cohort. METHODS: A total of 192 Chilean IBD patients were genotyped using Illuminas Global Screening Array. Genotype data were combined with similar information from 3,147 Chilean controls. The proportions of Aymara, African, European, and Mapuche ancestries were estimated using the software ADMIXTURE. We calculated the odds ratios (ORs) and 95% confidence intervals (CIs) for gender, age, and ancestry proportions. We also explored associations with previously reported IBD-risk variants independently and in conjunction with genetic ancestry. RESULTS: The first and third quartiles of the proportion of Mapuche ancestry in IBD patients were 24.7 and 34.2%, respectively, and the corresponding OR was 2.30 (95%CI 1.52-3.48) for the lowest vs. the highest group. Only one variant (rs7210086) of the 180 reported IBD-risk SNPs was associated with IBD risk in the Chilean cohort (adjusted P = 0.01). This variant is related to myeloid cells. CONCLUSION: The type and proportion of Native American ancestry in Chileans seem to be associated with IBD risk. Variants associated with IBD risk in this Andean region were related to myeloid cells and the innate immune response.
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- 2023
7. Epithelial HVEM maintains intraepithelial T cell survival and contributes to host protection.
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Seo, Goo-Young, Takahashi, Daisuke, Wang, Qingyang, Mikulski, Zbigniew, Chen, Angeline, Chou, Ting-Fang, Marcovecchio, Paola, McArdle, Sara, Sethi, Ashu, Shui, Jr-Wen, Takahashi, Masumi, Surh, Charles, Cheroutre, Hilde, and Kronenberg, Mitchell
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Animals ,Collagen ,Epithelial Cells ,Integrins ,Intraepithelial Lymphocytes ,Ligands ,Mice - Abstract
Intraepithelial T cells (IETs) are in close contact with intestinal epithelial cells and the underlying basement membrane, and they detect invasive pathogens. How intestinal epithelial cells and basement membrane influence IET survival and function, at steady state or after infection, is unclear. The herpes virus entry mediator (HVEM), a member of the TNF receptor superfamily, is constitutively expressed by intestinal epithelial cells and is important for protection from pathogenic bacteria. Here, we showed that at steady-state LIGHT, an HVEM ligand, binding to epithelial HVEM promoted the survival of small intestine IETs. RNA-seq and addition of HVEM ligands to epithelial organoids indicated that HVEM increased epithelial synthesis of basement membrane proteins, including collagen IV, which bound to β1 integrins expressed by IETs. Therefore, we proposed that IET survival depended on β1 integrin binding to collagen IV and showed that β1 integrin-collagen IV interactions supported IET survival in vitro. Moreover, the absence of β1 integrin expression by T lymphocytes decreased TCR αβ+ IETs in vivo. Intravital microscopy showed that the patrolling movement of IETs was reduced without epithelial HVEM. As likely consequences of decreased number and movement, protective responses to Salmonella enterica were reduced in mice lacking either epithelial HVEM, HVEM ligands, or β1 integrins. Therefore, IETs, at steady state and after infection, depended on HVEM expressed by epithelial cells for the synthesis of collagen IV by epithelial cells. Collagen IV engaged β1 integrins on IETs that were important for their maintenance and for their protective function in mucosal immunity.
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- 2022
8. Hypoxia induces adrenomedullin from lung epithelia, stimulating ILC2 inflammation and immunity.
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Han, Jihye, Wan, Qingqing, Seo, Goo-Young, Kim, Kenneth, El Baghdady, Sarah, Lee, Jee, Liu, Yun-Cai, and Kronenberg, Mitchell
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Adrenomedullin ,Epithelium ,Humans ,Hypoxia ,Immunity ,Innate ,Inflammation ,Lung ,Lung Diseases ,Lymphocytes - Abstract
Hypoxia contributes to airway inflammation and remodeling in several lung diseases; however, exactly how hypoxic pulmonary epithelium regulates allergic inflammation remains to be fully characterized. Here, we report that conditional deletion of the E3 ubiquitin ligase VHL in lung epithelial cells resulted in exacerbated type 2 responses accompanied by selective increase of group 2 innate lymphoid cells (ILC2s) at steady state and following inflammation or helminth infection. Ablation of expression of the hypoxia-inducible factor 2α (HIF2α) significantly reversed VHL-mediated ILC2 activation. VHL deficiency in lung epithelial cells caused increased expression of the peptide hormone adrenomedullin (ADM), and our data suggest that HIF2α controls Adm expression. ADM directly promoted ILC2 activation both in vitro and in vivo. Our findings indicate that the hypoxic response mediated by the VHL-HIF2α axis is critical for control of pulmonary type 2 responses by increasing ADM expression in lung epithelia, causing ILC2 activation.
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- 2022
9. Intermittent PI3Kδ inhibition sustains anti-tumour immunity and curbs irAEs.
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Eschweiler, Simon, Ramírez-Suástegui, Ciro, Li, Yingcong, King, Emma, Chudley, Lindsey, Thomas, Jaya, Wood, Oliver, von Witzleben, Adrian, Jeffrey, Danielle, McCann, Katy, Simon, Hayley, Mondal, Monalisa, Wang, Alice, Dicker, Martina, Lopez-Guadamillas, Elena, Chou, Ting-Fang, Dobbs, Nicola, Essame, Louisa, Acton, Gary, Kelly, Fiona, Halbert, Gavin, Sacco, Joseph, Schache, Andrew, Shaw, Richard, McCaul, James, Paterson, Claire, Davies, Joseph, Brennan, Peter, Singh, Rabindra, Loadman, Paul, Wilson, William, Hackshaw, Allan, Seumois, Gregory, Okkenhaug, Klaus, Thomas, Gareth, Jones, Terry, Ay, Ferhat, Friberg, Greg, Vanhaesebroeck, Bart, Vijayanand, Pandurangan, Ottensmeier, Christian, and Kronenberg, Mitchell
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Adenosine ,Animals ,Antineoplastic Agents ,Disease Models ,Animal ,Head and Neck Neoplasms ,Humans ,Immunotherapy ,Mice ,Phosphatidylinositol 3-Kinases ,Quinolines ,T-Lymphocytes ,Regulatory - Abstract
Phosphoinositide 3-kinase δ (PI3Kδ) has a key role in lymphocytes, and inhibitors that target this PI3K have been approved for treatment of B cell malignancies1-3. Although studies in mouse models of solid tumours have demonstrated that PI3Kδ inhibitors (PI3Kδi) can induce anti-tumour immunity4,5, its effect on solid tumours in humans remains unclear. Here we assessed the effects of the PI3Kδi AMG319 in human patients with head and neck cancer in a neoadjuvant, double-blind, placebo-controlled randomized phase II trial (EudraCT no. 2014-004388-20). PI3Kδ inhibition decreased the number of tumour-infiltrating regulatory T (Treg) cells and enhanced the cytotoxic potential of tumour-infiltrating T cells. At the tested doses of AMG319, immune-related adverse events (irAEs) required treatment to be discontinued in 12 out of 21 of patients treated with AMG319, suggestive of systemic effects on Treg cells. Accordingly, in mouse models, PI3Kδi decreased the number of Treg cells systemically and caused colitis. Single-cell RNA-sequencing analysis revealed a PI3Kδi-driven loss of tissue-resident colonic ST2 Treg cells, accompanied by expansion of pathogenic T helper 17 (TH17) and type 17 CD8+ T (TC17) cells, which probably contributed to toxicity; this points towards a specific mode of action for the emergence of irAEs. A modified treatment regimen with intermittent dosing of PI3Kδi in mouse models led to a significant decrease in tumour growth without inducing pathogenic T cells in colonic tissue, indicating that alternative dosing regimens might limit toxicity.
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- 2022
10. Integrative scATAC-seq and scRNA-seq analyses map thymic iNKT cell development and identify Cbfβ for its commitment
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Wang, Jie, Adrianto, Indra, Subedi, Kalpana, Liu, Tingting, Wu, Xiaojun, Yi, Qijun, Loveless, Ian, Yin, Congcong, Datta, Indrani, Sant’Angelo, Derek B., Kronenberg, Mitchell, Zhou, Li, and Mi, Qing-Sheng
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- 2023
- Full Text
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11. NKT cells in the antitumor response: the [beta] version?
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Kronenberg, Mitchell and Engel, Isaac
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T cells ,Membrane lipids ,Antigens ,Gangliosides ,Sulfates -- Product development ,Health care industry - Abstract
NKT cells recognize glycolipids presented by CD1d-expressing antigen-presenting cells (APCs) and include type I NKT cells with antitumor function and type II NKT cells, which have been reported to suppress the antitumor response. Some type II NKT cells recognize sulfatide, a glycosphingolipid with a sulfate modification of the sugar. Type I NKT cells recognize different glycosphingolipids. In this issue of theJCI, Nishio and colleagues showed that APCs could process sulfatide antigens, analogous to protein processing for peptide-reactive T cells. Antigen processing in lysosomes removed sulfate to generate a glycosphingolipid that stimulated type I NKT cells and thereby turned an antigen with no antitumor activity into one that not only stimulated type I NKT cells but also stimulated antitumor responses. These findings may extend to the development of glycolipid antigens that could stimulate anticancer responses via antigen processing by APCs., Type I and type II NKT cells recognize different glycolipids NKT cells, which are T lymphocytes that are distinct from innate immune NK cells, have been tested extensively for anticancer [...]
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- 2024
- Full Text
- View/download PDF
12. Stimulation of a subset of natural killer T cells by CD103+ DC is required for GM-CSF and protection from pneumococcal infection.
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Murray, Mallory, Crosby, Catherine, Marcovecchio, Paola, Hartmann, Nadine, Chandra, Shilpi, Zhao, Meng, Khurana, Archana, Zahner, Sonja, Clausen, Björn, Coleman, Fadie, Mizgerd, Joseph, Mikulski, Zbigniew, and Kronenberg, Mitchell
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Streptococcus pneumoniae ,T cell ,cytokine ,dendritic cell ,innate ,lung infection ,natural killer T cell ,γδ T cell ,Animals ,Cell Line ,Dendritic Cells ,Female ,Granulocyte-Macrophage Colony-Stimulating Factor ,Humans ,Interferon-gamma ,Interleukin-17 ,Intraepithelial Lymphocytes ,Lung ,Lymphocyte Activation ,Male ,Mice ,Mice ,Inbred C57BL ,Natural Killer T-Cells ,Pneumococcal Infections ,Receptors ,Antigen ,T-Cell ,gamma-delta ,Streptococcus pneumoniae - Abstract
Innate-like T cells, including invariant natural killer T cells, mucosal-associated invariant T cells, and γδ T cells, are present in various barrier tissues, including the lung, where they carry out protective responses during infections. Here, we investigate their roles during pulmonary pneumococcal infection. Following infection, innate-like T cells rapidly increase in lung tissue, in part through recruitment, but T cell antigen receptor activation and cytokine production occur mostly in interleukin-17-producing NKT17 and γδ T cells. NKT17 cells are preferentially located within lung tissue prior to infection, as are CD103+ dendritic cells, which are important both for antigen presentation to NKT17 cells and γδ T cell activation. Whereas interleukin-17-producing γδ T cells are numerous, granulocyte-macrophage colony-stimulating factor is exclusive to NKT17 cells and is required for optimal protection. These studies demonstrate how particular cellular interactions and responses of functional subsets of innate-like T cells contribute to protection from pathogenic lung infection.
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- 2022
13. Calcium signals regulate the functional differentiation of thymic iNKT cells
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Zhao, Meng, Quintana, Ariel, Zhang, Chen, Andreyev, Alexander Y, Kiosses, William, Kuwana, Tomomi, Murphy, Anne, Hogan, Patrick G, and Kronenberg, Mitchell
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Biochemistry and Cell Biology ,Biomedical and Clinical Sciences ,Biological Sciences ,Immunology ,Medical Physiology ,Underpinning research ,1.1 Normal biological development and functioning ,Animals ,Calcium ,Calcium Release Activated Calcium Channels ,Calcium Signaling ,Cell Differentiation ,Mice ,Inbred BALB C ,Mice ,Inbred C57BL ,Mitochondria ,Natural Killer T-Cells ,Thymus Gland ,calcium signaling ,iNKT cells ,metabolism ,mitochondria ,T-cell differentiation ,Information and Computing Sciences ,Medical and Health Sciences ,Developmental Biology ,Biological sciences ,Biomedical and clinical sciences - Abstract
How natural or innate-like lymphocytes generate the capacity to produce IL-4 and other cytokines characteristic of type 2 immunity remains unknown. Invariant natural killer T (iNKT) cells differentiate in the thymus into NKT1, NKT2, and NKT17 subsets, similar to mature, peripheral CD4+ T helper cells. The mechanism for this differentiation was not fully understood. Here, we show that NKT2 cells required higher and prolonged calcium (Ca2+ ) signals and continuing activity of the calcium release-activated calcium (CRAC) channel, than their NKT1 counterparts. The sustained Ca2+ entry via CRAC pathway in NKT2 cells was apparently mediated by ORAI and controlled in part by the large mitochondrial Ca2+ uptake. Unique properties of mitochondria in NKT2 cells, including high activity of oxidative phosphorylation, may regulate mitochondrial Ca2+ buffering in NKT2 cells. In addition, the low Ca2+ extrusion rate may also contribute to the higher Ca2+ level in NKT2 cells. Altogether, we identified ORAI-dependent Ca2+ signaling connected with mitochondria and cellular metabolism, as a central regulatory pathway for the differentiation of NKT2 cells.
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- 2021
14. Metabolic activation and colitis pathogenesis is prevented by lymphotoxin β receptor expression in neutrophils.
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Riffelmacher, Thomas, Giles, Daniel, Zahner, Sonja, Dicker, Martina, Andreyev, Alexander, McArdle, Sara, Perez-Jeldres, Tamara, van der Gracht, Esmé, Murray, Mallory, Hartmann, Nadine, Tumanov, Alexei, and Kronenberg, Mitchell
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Activation ,Metabolic ,Animals ,Colitis ,Dextran Sulfate ,Disease Models ,Animal ,Disease Progression ,Humans ,Inflammatory Bowel Diseases ,Lymphotoxin beta Receptor ,Mice ,Mice ,Inbred C57BL ,Mice ,Knockout ,Mitochondria ,Neutrophils ,Tumor Necrosis Factor Ligand Superfamily Member 14 - Abstract
Inflammatory bowel disease is characterized by an exacerbated intestinal immune response, but the critical mechanisms regulating immune activation remain incompletely understood. We previously reported that the TNF-superfamily molecule TNFSF14 (LIGHT) is required for preventing severe disease in mouse models of colitis. In addition, deletion of lymphotoxin beta receptor (LTβR), which binds LIGHT, also led to aggravated colitis pathogenesis. Here, we aimed to determine the cell type(s) requiring LTβR and the mechanism critical for exacerbation of colitis. Specific deletion of LTβR in neutrophils (LTβRΔN), but not in several other cell types, was sufficient to induce aggravated colitis and colonic neutrophil accumulation. Mechanistically, RNA-Seq analysis revealed LIGHT-induced suppression of cellular metabolism, and mitochondrial function, that was dependent on LTβR. Functional studies confirmed increased mitochondrial mass and activity, associated with excessive mitochondrial ROS production and elevated glycolysis at steady-state and during colitis. Targeting these metabolic changes rescued exacerbated disease severity. Our results demonstrate that LIGHT signals to LTβR on neutrophils to suppress metabolic activation and thereby prevents exacerbated immune pathogenesis during colitis.
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- 2021
15. Transcriptome and chromatin landscape of iNKT cells are shaped by subset differentiation and antigen exposure.
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Murray, Mallory, Engel, Isaac, Seumois, Grégory, Herrera-De la Mata, Sara, Rosales, Sandy, Sethi, Ashu, Logandha Ramamoorthy Premlal, Ashmitaa, Seo, Goo-Young, Greenbaum, Jason, Vijayanand, Pandurangan, Scott-Browne, James, and Kronenberg, Mitchell
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Animals ,Cell Differentiation ,Chromatin ,Female ,Lung ,Lymphocyte Activation ,Mice ,Mice ,Inbred C57BL ,Mice ,Transgenic ,Natural Killer T-Cells ,T Follicular Helper Cells ,T-Lymphocyte Subsets ,Thymus Gland ,Transcriptome - Abstract
Invariant natural killer T cells (iNKT cells) differentiate into thymic and peripheral NKT1, NKT2 and NKT17 subsets. Here we use RNA-seq and ATAC-seq analyses and show iNKT subsets are similar, regardless of tissue location. Lung iNKT cell subsets possess the most distinct location-specific features, shared with other innate lymphocytes in the lung, possibly consistent with increased activation. Following antigenic stimulation, iNKT cells undergo chromatin and transcriptional changes delineating two populations: one similar to follicular helper T cells and the other NK or effector like. Phenotypic analysis indicates these changes are observed long-term, suggesting that iNKT cells gene programs are not fixed, but they are capable of chromatin remodeling after antigen to give rise to additional subsets.
- Published
- 2021
16. Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis.
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Marki, Alex, Buscher, Konrad, Lorenzini, Cristina, Meyer, Matthew, Saigusa, Ryosuke, Fan, Zhichao, Yeh, Yi-Ting, Hartmann, Nadine, Dan, Jennifer M, Kiosses, William B, Golden, Gregory J, Ganesan, Rajee, Winkels, Holger, Orecchioni, Marco, McArdle, Sara, Mikulski, Zbigniew, Altman, Yoav, Bui, Jack, Kronenberg, Mitchell, Chien, Shu, Esko, Jeffrey D, Nizet, Victor, Smalley, David, Roth, Johannes, and Ley, Klaus
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Immunology ,Medical and Health Sciences - Abstract
Rolling neutrophils form tethers with submicron diameters. Here, we report that these tethers detach, forming elongated neutrophil-derived structures (ENDS) in the vessel lumen. We studied ENDS formation in mice and humans in vitro and in vivo. ENDS do not contain mitochondria, endoplasmic reticulum, or DNA, but are enriched for S100A8, S100A9, and 57 other proteins. Within hours of formation, ENDS round up, and some of them begin to present phosphatidylserine on their surface (detected by annexin-5 binding) and release S100A8-S100A9 complex, a damage-associated molecular pattern protein that is a known biomarker of neutrophilic inflammation. ENDS appear in blood plasma of mice upon induction of septic shock. Compared with healthy donors, ENDS are 10-100-fold elevated in blood plasma of septic patients. Unlike neutrophil-derived extracellular vesicles, most ENDS are negative for the tetraspanins CD9, CD63, and CD81. We conclude that ENDS are a new class of bloodborne submicron particles with a formation mechanism linked to neutrophil rolling on the vessel wall.
- Published
- 2021
17. The role of innate lymphoid cells in response to microbes at mucosal surfaces.
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Seo, Goo-Young, Giles, Daniel, and Kronenberg, Mitchell
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Animals ,Biomarkers ,Cytokines ,Homeostasis ,Host-Parasite Interactions ,Host-Pathogen Interactions ,Humans ,Immunity ,Innate ,Lymphocyte Subsets ,Microbiota ,Mucous Membrane - Abstract
Innate lymphoid cells (ILCs) are a lymphocyte population that is mostly resident at mucosal surfaces. They help to induce an appropriate immune response to the microbiome at homeostasis. In healthy people, the mucosal immune system works symbiotically with organisms that make up the microbiota. ILCs play a critical role in orchestrating this balance, as they can both influence and in turn be influenced by the microbiome. ILCs also are important regulators of the early response to infections by diverse types of pathogenic microbes at mucosal barriers. Their rapid responses initiate inflammatory programs, production of antimicrobial products and repair processes. This review will focus on the role of ILCs in response to the microbiota and to microbial infections of the lung and intestine.
- Published
- 2020
18. Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets.
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Seo, Goo-Young, Giles, Daniel, and Kronenberg, Mitchell
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Adoptive transfer ,Group 3 innate lymphoid cells ,Innate immunity ,Small intestine lamina propria ,Yersinia enterocolitica ,Adoptive Transfer ,Animals ,Homeodomain Proteins ,Immunity ,Innate ,Intestine ,Small ,Lymphocyte Subsets ,Mice ,Mice ,Knockout ,Mucous Membrane ,Yersinia Infections - Abstract
Innate lymphoid cells (ILCs) are important regulators of the early responses to infection at mucosal barriers, including the intestine. Recently, we have shown that specific ILC3 subsets protect against enteric bacterial pathogens. Here, we describe a mouse model of oral infection by Yersinia enterocolitica (Y. enterocolitica) and several different methodologies to assess the severity of the infection. We also detail how ILC3 subsets can be isolated from the mouse small intestine and transferred into recipient immune deficient mice to study the function of these ILCs in the small intestine.
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- 2020
19. HSV-1 latency and the kinetics of reactivation are regulated by a complex network of interactions between HVEM, its ligands (gD, BTLA, LIGHT and CD160) and LAT
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Wang, Shaohui, Ljubimov, Alexander V, Jin, Ling, Pfeffer, Klaus, Kronenberg, Mitchell, and Ghiasi, Homayon
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Neurosciences ,Infectious Diseases ,Sexually Transmitted Infections ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Animals ,Antigens ,CD ,Eye Diseases ,Female ,GPI-Linked Proteins ,Gene Knockout Techniques ,Herpes Simplex ,Herpesvirus 1 ,Human ,Kinetics ,Male ,Mice ,MicroRNAs ,Receptors ,Immunologic ,Receptors ,Tumor Necrosis Factor ,Member 14 ,Tumor Necrosis Factor Ligand Superfamily Member 14 ,Viral Envelope Proteins ,Virus Internalization ,Virus Latency ,Virus Replication ,ocular ,latency ,reactivation ,virus replication ,corneal scarring ,eye diseases ,Biological Sciences ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Virology - Abstract
Recently, we reported that the herpesvirus entry mediator (HVEM; also called TNFRSF14 or CD270) is upregulated by the latency-associated transcript (LAT) of herpes simplex virus 1 (HSV-1) and that the absence of HVEM affects latency reactivation but not primary infection in ocularly infected mice. gD has been shown to bind to HVEM. LIGHT (TNFSF14), CD160, and BTLA (B- and T-lymphocyte attenuator) also interact with HVEM and can interfere with HSV gD binding. It was not known if LIGHT, CD160, or BTLA affected the level of latency reactivation in the trigeminal ganglia (TG) of latently infected mice. To address this issue, we ocularly infected LIGHT-/-, CD160-/-, and BTLA-/- mice with LAT(+) and LAT(-) viruses, using similarly infected wild-type (WT) and HVEM-/- mice as controls. The amount of latency, as determined by the levels of gB DNA in the TG of the LIGHT-/-, CD160-/-, and BTLA-/- mice infected with either LAT(+) or LAT(-) viruses, was lower than that in WT mice infected with LAT(+) virus and was similar in WT mice infected with LAT(-) virus. The levels of LAT RNA in HVEM-/-, LIGHT-/-, CD160-/-, and BTLA-/- mice infected with LAT(+) virus were similar and were lower than the levels of LAT RNA in WT mice. However, LIGHT-/-, CD160-/-, and BTLA-/- mice, independent of the presence of LAT, had levels of reactivation similar to those of WT mice infected with LAT(+) virus. Faster reactivation correlated with the upregulation of HVEM transcript. The LIGHT-/-, CD160-/-, and BTLA-/- mice had higher levels of HVEM expression, and this, along with the absence of BTLA, LIGHT, or CD160, may contribute to faster reactivation, while the absence of each molecule, independent of LAT, may have contributed to lower latency. This study suggests that, in the absence of competition with gD for binding to HVEM, LAT RNA is important for WT levels of latency but not for WT levels of reactivation.IMPORTANCE The effects of BTLA, LIGHT, and CD160 on latency reactivation are not known. We show here that in BTLA, LIGHT, or CD160 null mice, latency is reduced; however, HVEM expression is upregulated compared to that of WT mice, and this upregulation is associated with higher reactivation that is independent of LAT but dependent on gD expression. Thus, one of the mechanisms by which BTLA, LIGHT, and CD160 null mice enhance reactivation appears to be the increased expression of HVEM in the presence of gD. Thus, our results suggest that blockade of HVEM-LIGHT-BTLA-CD160 contributes to reduced HSV-1 latency and reactivation.
- Published
- 2018
20. Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae.
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Chandra, Shilpi, Gray, James, Kiosses, William, Khurana, Archana, Hitomi, Kaori, Crosby, Catherine, Chawla, Ashu, Fu, Zheng, Zhao, Meng, Veerapen, Natacha, Richardson, Stewart, Porcelli, Steven, Besra, Gurdyal, Howell, Amy, Sharma, Sonia, Peters, Bjoern, and Kronenberg, Mitchell
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ATP Binding Cassette Transporter ,Subfamily B ,Member 1 ,Animals ,Antigen Presentation ,Antigens ,CD1d ,Cell Line ,Endosomes ,Gene Regulatory Networks ,Lipids ,Lymphocyte Activation ,Lysosomes ,Macrophages ,Membrane Microdomains ,Mice ,Inbred BALB C ,Mice ,Knockout ,Natural Killer T-Cells ,RNA ,Small Interfering ,Streptococcus pneumoniae - Abstract
Invariant natural killer T cells (iNKT cells) are activated by lipid antigens presented by CD1d, but the pathway leading to lipid antigen presentation remains incompletely characterized. Here we show a whole-genome siRNA screen to elucidate the CD1d presentation pathway. A majority of gene knockdowns that diminish antigen presentation reduced formation of glycolipid-CD1d complexes on the cell surface, including members of the HOPS and ESCRT complexes, genes affecting cytoskeletal rearrangement, and ABC family transporters. We validated the role in vivo for the multidrug resistance protein 1 (Mrp1) in CD1d antigen presentation. Mrp1 deficiency reduces surface clustering of CD1d, which decreased iNKT cell activation. Infected Mrp1 knockout mice show decreased iNKT cell responses to antigens from Streptococcus pneumoniae and were associated with increased mortality. Our results highlight the unique cellular events involved in lipid antigen presentation and show how modification of this pathway can lead to lethal infection.
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- 2018
21. Role of MAIT cells in pulmonary bacterial infection.
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Hartmann, Nadine, Harriff, Melanie, McMurtrey, Curtis, Hildebrand, William, Lewinsohn, David, and Kronenberg, Mitchell
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Innate immunity ,MAIT cells ,MR1 ,Pulmonary infection ,Animals ,Anti-Bacterial Agents ,Antigen Presentation ,Bacterial Infections ,Humans ,Lung ,Lymphocyte Activation ,Mucosal-Associated Invariant T Cells - Abstract
Mucosal-associated invariant T (MAIT) cells represent a population of innate T cells that is highly abundant in humans. MAIT cells recognize metabolites of the microbial vitamin B pathway that are presented by the major histocompatibility complex (MHC) class I-related protein MR1. Upon bacterial infection, activated MAIT cells produce diverse cytokines and cytotoxic effector molecules and accumulate at the site of infection, thus, MAIT cells have been shown to be protective against various bacterial infections. Here, we summarize the current knowledge of the role of MAIT cells in bacterial pulmonary infection models.
- Published
- 2018
22. LIGHT-HVEM Signaling in Innate Lymphoid Cell Subsets Protects Against Enteric Bacterial Infection.
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Seo, Goo-Young, Shui, Jr-Wen, Takahashi, Daisuke, Song, Christina, Wang, Qingyang, Kim, Kenneth, Mikulski, Zbigniew, Chandra, Shilpi, Giles, Daniel, Zahner, Sonja, Kim, Pyeung-Hyeun, Cheroutre, Hilde, Colonna, Marco, and Kronenberg, Mitchell
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CCR6 ,HVEM ,IFN-γ ,LIGHT ,Yersinia enterocolitica ,ileum ,infection ,innate lymphoid cells ,Adoptive Transfer ,Adult ,Animals ,Cytokines ,Disease Models ,Animal ,Enterobacteriaceae Infections ,Homeodomain Proteins ,Host-Pathogen Interactions ,Humans ,Interferon-gamma ,Lymphocytes ,Male ,Mice ,Mice ,Inbred C57BL ,Mice ,Knockout ,Neuropeptides ,Protein Transport ,Receptors ,CCR6 ,Receptors ,Tumor Necrosis Factor ,Receptors ,Tumor Necrosis Factor ,Member 14 ,Signal Transduction ,Spleen ,Tumor Necrosis Factor Ligand Superfamily Member 14 ,Yersinia enterocolitica - Abstract
Innate lymphoid cells (ILCs) are important regulators of early infection at mucosal barriers. ILCs are divided into three groups based on expression profiles, and are activated by cytokines and neuropeptides. Yet, it remains unknown if ILCs integrate other signals in providing protection. We show that signaling through herpes virus entry mediator (HVEM), a member of the tumor necrosis factor (TNF) receptor superfamily, in ILC3 is important for host defense against oral infection with the bacterial pathogen Yersinia enterocolitica. HVEM stimulates protective interferon-γ (IFN-γ) secretion from ILCs, and mice with HVEM-deficient ILC3 exhibit reduced IFN-γ production, higher bacterial burdens and increased mortality. In addition, IFN-γ production is critical as adoptive transfer of wild-type but not IFN-γ-deficient ILC3 can restore protection to mice lacking ILCs. We identify the TNF superfamily member, LIGHT, as the ligand inducing HVEM signals in ILCs. Thus HVEM signaling mediated by LIGHT plays a critical role in regulating ILC3-derived IFN-γ production for protection following infection. VIDEO ABSTRACT.
- Published
- 2018
23. Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70.
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Zhao, Meng, Svensson, Mattias ND, Venken, Koen, Chawla, Ashu, Liang, Shu, Engel, Isaac, Mydel, Piotr, Day, Jeremy, Elewaut, Dirk, Bottini, Nunzio, and Kronenberg, Mitchell
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Synovial Fluid ,Spleen ,Thymus Gland ,Lymphocyte Subsets ,Animals ,Mice ,Inbred BALB C ,Arthritis ,Disease Progression ,Receptors ,Cell Surface ,Receptors ,Antigen ,T-Cell ,Organ Culture Techniques ,Signal Transduction ,Cell Differentiation ,Phenotype ,Mutation ,Principal Component Analysis ,ZAP-70 Protein-Tyrosine Kinase ,Interferon-gamma ,Natural Killer T-Cells ,Mice ,Inbred BALB C ,Receptors ,Antigen ,T-Cell ,Cell Surface - Abstract
Various subsets of invariant natural killer T (iNKT) cells with different cytokine productions develop in the mouse thymus, but the factors driving their differentiation remain unclear. Here we show that hypomorphic alleles of Zap70 or chemical inhibition of Zap70 catalysis leads to an increase of IFN-γ-producing iNKT cells (NKT1 cells), suggesting that NKT1 cells may require a lower TCR signal threshold. Zap70 mutant mice develop IL-17-dependent arthritis. In a mouse experimental arthritis model, NKT17 cells are increased as the disease progresses, while NKT1 numbers negatively correlates with disease severity, with this protective effect of NKT1 linked to their IFN-γ expression. NKT1 cells are also present in the synovial fluid of arthritis patients. Our data therefore suggest that TCR signal strength during thymic differentiation may influence not only IFN-γ production, but also the protective function of iNKT cells in arthritis.
- Published
- 2018
24. Development of Asthma in Inner-City Children: Possible Roles of MAIT Cells and Variation in the Home Environment
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Chandra, Shilpi, Wingender, Gerhard, Greenbaum, Jason A, Khurana, Archana, Gholami, Amin M, Ganesan, Anusha-Preethi, Rosenbach, Michael, Jaffee, Katy, Gern, James E, Wood, Robert, O'Connor, George, Sandel, Megan, Kattan, Meyer, Bacharier, Leonard, Togias, Alkis, Horner, Anthony A, and Kronenberg, Mitchell
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Biochemistry and Cell Biology ,Biomedical and Clinical Sciences ,Biological Sciences ,Immunology ,Lung ,Clinical Research ,Asthma ,Pediatric ,Prevention ,Respiratory ,CD4-Positive T-Lymphocytes ,Child ,Child ,Preschool ,Cities ,Cohort Studies ,Dust ,Environment ,Female ,Humans ,Infant ,Interferon-gamma ,Lymphocyte Activation ,Lymphocyte Count ,Male ,Mucosal-Associated Invariant T Cells ,Natural Killer T-Cells ,Risk ,Biochemistry and cell biology - Abstract
Humans have populations of innate-like T lymphocytes with an invariant TCR α-chain that recognize nonpeptide Ags, including invariant NKT (iNKT) cells and mucosal-associated invariant T (MAIT) cells. iNKT cell involvement in human asthma is controversial, whereas there has been little analysis of MAIT cells. Using peripheral blood cells from 110 participants from the Urban Environment and Childhood Asthma (URECA) birth cohort study, these cells were analyzed for number and function. We determined whether iNKT cell or MAIT cell frequency at 1 y is correlated with the cytokine polarization of mainstream CD4+ T cells and/or the development of asthma by age 7 y. Dust samples from 300 houses were tested for iNKT cell antigenic activity. Our results show that a higher MAIT cell frequency at 1 y of age was associated with a decreased risk of asthma by age 7 y. The frequency of MAIT cells was associated with increased production of IFN-γ by activated CD4+ T cells from the URECA cohort. iNKT cell antigenic activity in bedroom dust samples was associated with higher endotoxin concentration and also with reduced risk of asthma. In conclusion, MAIT cell frequency at 1 y may reflect the tendency of the immune system toward Th1 responses and is associated with protection from asthma. Additionally, iNKT cell antigenic activity may be a marker of houses with increased microbial exposures and therefore also with protection from asthma.
- Published
- 2018
25. NKT cells in the antitumor response: the β version?
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Kronenberg, Mitchell, primary and Engel, Isaac, additional
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- 2024
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26. Promoter-interacting expression quantitative trait loci are enriched for functional genetic variants
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Chandra, Vivek, Bhattacharyya, Sourya, Schmiedel, Benjamin J., Madrigal, Ariel, Gonzalez-Colin, Cristian, Fotsing, Stephanie, Crinklaw, Austin, Seumois, Gregory, Mohammadi, Pejman, Kronenberg, Mitchell, Peters, Bjoern, Ay, Ferhat, and Vijayanand, Pandurangan
- Published
- 2021
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27. Transcriptomes and metabolism define mouse and human MAIT cell populations
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Chandra, Shilpi, primary, Ascui, Gabriel, additional, Riffelmacher, Thomas, additional, Chawla, Ashu, additional, Ramírez-Suástegui, Ciro, additional, Castelan, Viankail C., additional, Seumois, Gregory, additional, Simon, Hayley, additional, Murray, Mallory P., additional, Seo, Goo-Young, additional, Premlal, Ashmitaa L. R., additional, Schmiedel, Benjamin, additional, Verstichel, Greet, additional, Li, Yingcong, additional, Lin, Chia-Hao, additional, Greenbaum, Jason, additional, Lamberti, John, additional, Murthy, Raghav, additional, Nigro, John, additional, Cheroutre, Hilde, additional, Ottensmeier, Christian H., additional, Hedrick, Stephen M., additional, Lu, Li-Fan, additional, Vijayanand, Pandurangan, additional, and Kronenberg, Mitchell, additional
- Published
- 2023
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28. The ImmGen consortium OpenSource T cell project
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Zemmour, David, Goldrath, Ananda, Kronenberg, Mitchell, Kang, Joonsoo, and Benoist, Christophe
- Published
- 2022
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29. Antigen specificity of invariant natural killer T-cells.
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Birkholz, Alysia and Kronenberg, Mitchell
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Glycolipid ,Immune system ,Natural killer T-cells ,Animals ,Antigens ,Bacterial ,Antigens ,CD1d ,Antigens ,Fungal ,Antigens ,Protozoan ,Epitopes ,Humans ,Natural Killer T-Cells - Abstract
Natural killer T-cells, with an invariant T-cell antigen receptor α-chain (iNKT cells), are unique and conserved subset of lymphocytes capable of altering the immune system through their rapid and potent cytokine responses. They are reactive to lipid antigens presented by the CD1d molecule, an antigen-presenting molecule that is not highly polymorphic. iNKT cell responses frequently involve mixtures of cytokines that work against each other, and therefore attempts are underway to develop synthetic antigens that elicit only strong interferon-gamma (IFNγ) or only strong interleukin-4 responses but not both. Strong IFNγ responses may correlate with tighter binding to CD1d and prolonged stimulation of iNKT cells, and this may be useful for vaccine adjuvants and for stimulating anti-tumor responses. iNKT cells are self-reactive although the structure of the endogenous antigen is controversial. By contrast, bacterial and fungal lipids that engage the T-cell receptor and activate IFNγ from iNKT cells have been identified from both pathogenic and commensal organisms and the responses are in some cases highly protective from pathogens in mice. It is possible that the expanding knowledge of iNKT cell antigens and iNKT cell activation will provide the basis for therapies for patients suffering from infectious and immune diseases and cancer.
- Published
- 2015
30. IL-27 regulates the differentiation of follicular helper NKT cells via metabolic adaptation of mitochondria.
- Author
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Yasuhiro Kamii, Koji Hayashizaki, Toshio Kanno, Akio Chiba, Taku Ikegami, Mitsuru Saito, Yukihiro Akeda, Toshiaki Ohteki, Masato Kubo, Kiyotsugu Yoshida, Kazuyoshi Kawakami, Kazunori Oishi, Araya, Jun, Kazuyoshi Kuwano, Kronenberg, Mitchell, Yusuke Endo, and Yuki Kinjo
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T helper cells ,T cell receptors ,OVARIAN follicle ,CELL differentiation ,T cells ,STREPTOCOCCUS pneumoniae ,IMMUNE response - Abstract
Invariant natural killer T (iNKT) cells are innate-like T lymphocytes that express an invariant T cell receptor α chain and contribute to bridging innate and acquired immunity with rapid production of large amounts of cytokines after stimulation. Among effecter subsets of iNKT cells, follicular helper NKT (NKT
FH ) cells are specialized to help B cells. However, the mechanisms of NKTFH cell differentiation remain to be elucidated. In this report, we studied the mechanism of NKTFH cell differentiation induced by pneumococcal surface protein A and α-galactosylceramide (P/A) vaccination. We found that Gr-1+ cells helped iNKT cell proliferation and NKTFH cell differentiation in the spleen by producing interleukin-27 (IL-27) in the early phase after vaccination. The neutralization of IL-27 impaired NKTFH cell differentiation, which resulted in compromised antibody production and diminished protection against Streptococcus pneumoniae infection by the P/A vaccine. Our data indicated that Gr-1+ cell-derived IL-27 stimulated mitochondrial metabolism, meeting the energic demand required for iNKT cells to differentiate into NKTFH cells. Interestingly, Gr-1+ cell-derived IL-27 was induced by iNKT cells via interferon-γ production. Collectively, our findings suggest that optimizing the metabolism of iNKT cells was essential for acquiring specific effector functions, and they provide beneficial knowledge on iNKT cell-mediated vaccination-mediated therapeutic strategies. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
31. Amerindian ancestry proportion as a risk factor for inflammatory bowel diseases: results from a Latin American Andean cohort
- Author
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Pérez-Jeldres, Tamara, primary, Magne, Fabien, additional, Ascui, Gabriel, additional, Alvares, Danilo, additional, Orellana, Matias, additional, Alvarez-Lobos, Manuel, additional, Hernandez-Rocha, Cristian, additional, Azocar, Lorena, additional, Aguilar, Nataly, additional, Espino, Alberto, additional, Estela, Ricardo, additional, Escobar, Sergio, additional, Zazueta, Alejandra, additional, Baez, Pablo, additional, Silva, Verónica, additional, De La Vega, Andres, additional, Arriagada, Elizabeth, additional, Pavez-Ovalle, Carolina, additional, Díaz-Asencio, Alejandro, additional, Travisany, Dante, additional, Miquel, Juan Francisco, additional, Villablanca, Eduardo J., additional, Kronenberg, Mitchell, additional, and Bustamante, María Leonor, additional
- Published
- 2023
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32. Innate‐like T cells: connecting the dots linking microscopic intestinal inflammation to spondyloarthritis
- Author
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Zhao, Meng, primary and Kronenberg, Mitchell, additional
- Published
- 2023
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33. Cancer immunity thwarted by the microbiome
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Hartmann, Nadine and Kronenberg, Mitchell
- Published
- 2018
34. The Role of Invariant Natural Killer T Cells in Autoimmune Diseases
- Author
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Wingender, Gerhard, primary and Kronenberg, Mitchell, additional
- Published
- 2020
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35. List of Contributors
- Author
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Abramson, Jakub, primary, Ahmed, S. Sohail, additional, Alba, Marco A., additional, Ali, Youssif M., additional, Ambrus, Julian L., additional, Andersson Svärd, Agnes, additional, Aringer, Martin, additional, Assassi, Shervin, additional, Aung, Thanda, additional, Ayzenberg, Ilya, additional, Barker, Robert N., additional, Baxter, Alan G., additional, Betterle, Corrado, additional, Birlea, Stanca A., additional, Björkström, Niklas K., additional, Blair, Paul A., additional, Blüml, Stephan, additional, Bosch, Xavier, additional, Brodsky, Robert A., additional, Bryceson, Yenan T., additional, Burkett, Patrick R., additional, Bussel, James B., additional, Caricchio, Roberto, additional, Casciola-Rosen, Livia, additional, Caturegli, Patrizio, additional, Chaigne-Delalande, Benjamin, additional, Chalan, Paulina, additional, Chatenoud, Lucienne, additional, Cohen, Philip L., additional, Cooper, Megan A., additional, Coppieters, Ken, additional, Crystal, Ronald G., additional, Culton, Donna A., additional, Damato, Valentina, additional, Davidson, Anne, additional, Delfino, Lorenzo, additional, Delves, Peter J., additional, Di Dalmazi, Giulia, additional, Diamond, Betty, additional, Diaz, Luis A., additional, Falk, Ronald J., additional, Fritzler, Marvin J., additional, Gallucci, Stefania, additional, Gangaputra, Sapna, additional, Gelbman, Brian, additional, Gershwin, M. Eric, additional, Gery, Igal, additional, Getts, Daniel R., additional, Gold, Ralf, additional, Goldfarb, Yael, additional, Gong, Jing, additional, Gordon, Siamon, additional, Goronzy, Jörg J., additional, Greer, Judith M., additional, Guazzone, Vanesa A., additional, Guilherme, Luiza, additional, Hafler, David A., additional, Hahn, Bevra H., additional, Hamad, Abdel Rahim A., additional, Hamano, Hideaki, additional, Harrison, Leonard C., additional, Homann, Dirk, additional, Husebye, Eystein S., additional, Jennette, J. Charles, additional, Jones, Richard J., additional, Jordan, Margaret A., additional, Kalil, Jorge, additional, Kawa, Shigeyuki, additional, Kaya, Ziya, additional, Keller, Christian W., additional, King, Nicholas J.C., additional, Kitcharoensakkul, Maleewan, additional, Kiyosawa, Kendo, additional, Königs, Christoph, additional, Kronenberg, Mitchell, additional, Kuchroo, Vijay K., additional, Laurence, Arian, additional, Lee, Eun-Ju, additional, Lehmann, Helmar C., additional, Lernmark, Åke, additional, Lindbladh, Ida, additional, Liu, Zhi, additional, Ljunggren, Hans-Gustaf, additional, Lunardi, Claudio, additional, Lundin, Knut E.A., additional, Lünemann, Jan D., additional, Lunn, Michael P.T., additional, Lustig, Livia, additional, Mackay, Charles R., additional, Mackay, Ian R., additional, Malattia, Clara, additional, Martinez-Pomares, Luisa, additional, Martini, Alberto, additional, Mauri, Claudia, additional, McCombe, Pamela A., additional, Melchers, Fritz, additional, Mieli-Vergani, Giorgina, additional, Miller, Frederick W., additional, Miller, Stephen D., additional, Mizui, Masayuki, additional, Mjösberg, Jenny, additional, Münz, Christian, additional, Nijjar, Jagtar Singh, additional, Norris, David A., additional, Oleinika, Kristine, additional, Oppenheim, Joost J., additional, Pawlak, Mathias, additional, Peligero-Cruz, Cristina, additional, Peters, Anneli, additional, Peterson, Pärt, additional, Pitarokoili, Kalliopi, additional, Presotto, Fabio, additional, Puccetti, Antonio, additional, Rabb, Hamid, additional, Raczek, Patricia, additional, Rahman, M. Jubayer, additional, Ramos-Casals, Manuel, additional, Rose, Noel R., additional, Rosen, Antony, additional, Sadasivam, Mohanraj, additional, Schiffenbauer, Adam, additional, Schwaeble, Wilhelm J., additional, Sen, H. Nida, additional, Serota, Marc, additional, Sheikh, Kazim A., additional, Shoenfeld, Yehuda, additional, Shovman, Ora, additional, Sieper, Joachim, additional, Silverstein, Arthur M., additional, Sim, Robert B., additional, Smith, Kenneth G C, additional, Smolen, Josef S., additional, Sollid, Ludvig M., additional, Spiteri, Alanna, additional, Steinman, Lawrence, additional, Stone, John H., additional, Syrbe, Uta, additional, Tamhaney, Ami, additional, Tanaka, Atsushi, additional, Taneja, Veena, additional, Tarbell, Kristin V., additional, Tinazzi, Elisa, additional, Tiong, Benedict K., additional, Toh, Ban-Hock, additional, Tsokos, George C., additional, Tung, Kenneth S.K., additional, Varga, John, additional, Vergani, Diego, additional, Vickers, Mark A., additional, Viegas, Stuart, additional, Vincent, Angela, additional, von Herrath, Matthias, additional, Weetman, Anthony P., additional, Weinstock, Joel V., additional, Wentworth, John M., additional, Wesley, Sarah, additional, Weyand, Cornelia M., additional, Wingender, Gerhard, additional, Winter, Michael W., additional, Zanchetta, Renato, additional, and Zouali, Moncef, additional
- Published
- 2020
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36. Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets
- Author
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Seo, Goo-Young, primary, Giles, Daniel A., additional, and Kronenberg, Mitchell, additional
- Published
- 2020
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37. Reduced expression of phosphatase PTPN2 promotes pathogenic conversion of Tregs in autoimmunity
- Author
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Svensson, Mattias N.D., Doody, Karen M., Schmiedel, Benjamin J., Bhattacharyya, Sourya, Panwar, Bharat, Wiede, Florian, Yang, Shen, Santelli, Eugenio, Wu, Dennis J., Sacchetti, Cristiano, Gujar, Ravindra, Seumois, Gregory, Kiosses, William B., Aubry, Isabelle, Kim, Gisen, Mydel, Piotr, Sakaguchi, Shimon, Kronenberg, Mitchell, Tiganis, Tony, Tremblay, Michel L., Ay, Ferhat, Vijayanand, Pandurangan, and Bottini, Nunzio
- Subjects
Autoimmunity -- Research ,Gene expression -- Research ,Phosphatases -- Research ,Rheumatoid arthritis -- Genetic aspects ,T cells -- Research ,DNA binding proteins ,Rheumatoid factor ,Arthritis ,Phenols (Class of compounds) ,Cytokines ,B cells ,Tyrosine ,Chromatin ,Autoimmune diseases ,Genes ,Biochemistry ,Health care industry - Abstract
Genetic variants at the PTPN2 locus, which encodes the tyrosine phosphatase PTPN2, cause reduced gene expression and are linked to rheumatoid arthritis (RA) and other autoimmune diseases. PTPN2 inhibits signaling through the T cell and cytokine receptors, and loss of PTPN2 promotes T cell expansion and CD4- and [CD8.sup.- ]driven autoimmunity. However, it remains unknown whether loss of PTPN2 in [FoxP3.sup.+] regulatory T cells (Tregs) plays a role in autoimmunity. Here we aimed to model human autoimmune-predisposing PTPN2 variants, the presence of which results in a partial loss of PTPN2 expression, in mouse models of RA. We identified that reduced expression of Ptpn2 enhanced the severity of autoimmune arthritis in the T cell-dependent SKG mouse model and demonstrated that this phenotype was mediated through a Treg-intrinsic mechanism. Mechanistically, we found that through dephosphorylation of STAT3, PTPN2 inhibits IL-6-driven pathogenic loss of FoxP3 after Tregs have acquired ROR[gamma]t expression, at a stage when chromatin accessibility for STAT3-targeted [IL-17.sup.-]associated transcription factors is maximized. We conclude that PTPN2 promotes FoxP3 stability in mouse [ROR[gamma]t.sup.+] Tregs and that loss of function of PTPN2 in Tregs contributes to the association between PTPN2 and autoimmunity., Introduction Rheumatoid arthritis (RA) is a chronic autoimmune, systemic inflammatory disorder that primarily affects diarthrodial joints (1). To date, various genome-wide association studies have identified over 100 risk loci for [...]
- Published
- 2019
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38. Production of α-galactosylceramide by a prominent member of the human gut microbiota.
- Author
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Wieland Brown, Laura C, Penaranda, Cristina, Kashyap, Purna C, Williams, Brianna B, Clardy, Jon, Kronenberg, Mitchell, Sonnenburg, Justin L, Comstock, Laurie E, Bluestone, Jeffrey A, and Fischbach, Michael A
- Subjects
Cells ,Cultured ,Animals ,Humans ,Mice ,Bacteroides fragilis ,Galactosylceramides ,Mutation ,Natural Killer T-Cells ,Cells ,Cultured ,2.1 Biological and endogenous factors ,Developmental Biology ,Biological Sciences ,Medical and Health Sciences ,Agricultural and Veterinary Sciences - Abstract
While the human gut microbiota are suspected to produce diffusible small molecules that modulate host signaling pathways, few of these molecules have been identified. Species of Bacteroides and their relatives, which often comprise >50% of the gut community, are unusual among bacteria in that their membrane is rich in sphingolipids, a class of signaling molecules that play a key role in inducing apoptosis and modulating the host immune response. Although known for more than three decades, the full repertoire of Bacteroides sphingolipids has not been defined. Here, we use a combination of genetics and chemistry to identify the sphingolipids produced by Bacteroides fragilis NCTC 9343. We constructed a deletion mutant of BF2461, a putative serine palmitoyltransferase whose yeast homolog catalyzes the committed step in sphingolipid biosynthesis. We show that the Δ2461 mutant is sphingolipid deficient, enabling us to purify and solve the structures of three alkaline-stable lipids present in the wild-type strain but absent from the mutant. The first compound was the known sphingolipid ceramide phosphorylethanolamine, and the second was its corresponding dihydroceramide base. Unexpectedly, the third compound was the glycosphingolipid α-galactosylceramide (α-GalCer(Bf)), which is structurally related to a sponge-derived sphingolipid (α-GalCer, KRN7000) that is the prototypical agonist of CD1d-restricted natural killer T (iNKT) cells. We demonstrate that α-GalCer(Bf) has similar immunological properties to KRN7000: it binds to CD1d and activates both mouse and human iNKT cells both in vitro and in vivo. Thus, our study reveals BF2461 as the first known member of the Bacteroides sphingolipid pathway, and it indicates that the committed steps of the Bacteroides and eukaryotic sphingolipid pathways are identical. Moreover, our data suggest that some Bacteroides sphingolipids might influence host immune homeostasis.
- Published
- 2013
39. Production of α-Galactosylceramide by a Prominent Member of the Human Gut Microbiota
- Author
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Brown, Laura C Wieland, Penaranda, Cristina, Kashyap, Purna C, Williams, Brianna B, Clardy, Jon, Kronenberg, Mitchell, Sonnenburg, Justin L, Comstock, Laurie E, Bluestone, Jeffrey A, and Fischbach, Michael A
- Subjects
Microbiology ,Biochemistry and Cell Biology ,Biological Sciences ,Aetiology ,2.1 Biological and endogenous factors ,Animals ,Bacteroides fragilis ,Cells ,Cultured ,Galactosylceramides ,Humans ,Mice ,Mutation ,Natural Killer T-Cells ,Agricultural and Veterinary Sciences ,Medical and Health Sciences ,Developmental Biology ,Agricultural ,veterinary and food sciences ,Biological sciences ,Biomedical and clinical sciences - Abstract
While the human gut microbiota are suspected to produce diffusible small molecules that modulate host signaling pathways, few of these molecules have been identified. Species of Bacteroides and their relatives, which often comprise >50% of the gut community, are unusual among bacteria in that their membrane is rich in sphingolipids, a class of signaling molecules that play a key role in inducing apoptosis and modulating the host immune response. Although known for more than three decades, the full repertoire of Bacteroides sphingolipids has not been defined. Here, we use a combination of genetics and chemistry to identify the sphingolipids produced by Bacteroides fragilis NCTC 9343. We constructed a deletion mutant of BF2461, a putative serine palmitoyltransferase whose yeast homolog catalyzes the committed step in sphingolipid biosynthesis. We show that the Δ2461 mutant is sphingolipid deficient, enabling us to purify and solve the structures of three alkaline-stable lipids present in the wild-type strain but absent from the mutant. The first compound was the known sphingolipid ceramide phosphorylethanolamine, and the second was its corresponding dihydroceramide base. Unexpectedly, the third compound was the glycosphingolipid α-galactosylceramide (α-GalCer(Bf)), which is structurally related to a sponge-derived sphingolipid (α-GalCer, KRN7000) that is the prototypical agonist of CD1d-restricted natural killer T (iNKT) cells. We demonstrate that α-GalCer(Bf) has similar immunological properties to KRN7000: it binds to CD1d and activates both mouse and human iNKT cells both in vitro and in vivo. Thus, our study reveals BF2461 as the first known member of the Bacteroides sphingolipid pathway, and it indicates that the committed steps of the Bacteroides and eukaryotic sphingolipid pathways are identical. Moreover, our data suggest that some Bacteroides sphingolipids might influence host immune homeostasis.
- Published
- 2013
40. Helicobacter pylori cholesteryl α-glucosides contribute to its pathogenicity and immune response by natural killer T cells.
- Author
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Ito, Yuki, Vela, Jose Luis, Matsumura, Fumiko, Hoshino, Hitomi, Tyznik, Aaron, Lee, Heeseob, Girardi, Enrico, Zajonc, Dirk M, Liddington, Robert, Kobayashi, Motohiro, Bao, Xingfeng, Bugaytsova, Jeanna, Borén, Thomas, Jin, Rongsheng, Zong, Yinong, Seeberger, Peter H, Nakayama, Jun, Kronenberg, Mitchell, and Fukuda, Minoru
- Subjects
Th1 Cells ,Th2 Cells ,Animals ,Mice ,Knockout ,Humans ,Mice ,Helicobacter pylori ,Helicobacter Infections ,Gastritis ,Atrophic ,Glucosides ,Phagocytosis ,Adolescent ,Adult ,Aged ,Aged ,80 and over ,Middle Aged ,Child ,Female ,Male ,Natural Killer T-Cells ,General Science & Technology - Abstract
Approximately 10-15% of individuals infected with Helicobacter pylori will develop ulcer disease (gastric or duodenal ulcer), while most people infected with H. pylori will be asymptomatic. The majority of infected individuals remain asymptomatic partly due to the inhibition of synthesis of cholesteryl α-glucosides in H. pylori cell wall by α1,4-GlcNAc-capped mucin O-glycans, which are expressed in the deeper portion of gastric mucosa. However, it has not been determined how cholesteryl α-glucosyltransferase (αCgT), which forms cholesteryl α-glucosides, functions in the pathogenesis of H. pylori infection. Here, we show that the activity of αCgT from H. pylori clinical isolates is highly correlated with the degree of gastric atrophy. We investigated the role of cholesteryl α-glucosides in various aspects of the immune response. Phagocytosis and activation of dendritic cells were observed at similar degrees in the presence of wild-type H. pylori or variants harboring mutant forms of αCgT showing a range of enzymatic activity. However, cholesteryl α-glucosides were recognized by invariant natural killer T (iNKT) cells, eliciting an immune response in vitro and in vivo. Following inoculation of H. pylori harboring highly active αCgT into iNKT cell-deficient (Jα18(-/-)) or wild-type mice, bacterial recovery significantly increased in Jα18(-/-) compared to wild-type mice. Moreover, cytokine production characteristic of Th1 and Th2 cells dramatically decreased in Jα18(-/-) compared to wild-type mice. These findings demonstrate that cholesteryl α-glucosides play critical roles in H. pylori-mediated gastric inflammation and precancerous atrophic gastritis.
- Published
- 2013
41. Mesenteric B Cells Centrally Inhibit CD4 + T Cell Colitis through Interaction with Regulatory T Cell Subsets
- Author
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Wei, Bo, Velazquez, Peter, Turovskaya, Olga, Spricher, Karsten, Aranda, Richard, Kronenberg, Mitchell, Birnbaumer, Lutz, and Braun, Jonathan
- Published
- 2005
42. Bacterial Glycolipids and Analogs as Antigens for CD1d-Restricted NKT Cells
- Author
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Wu, Douglass, Xing, Guo-Wen, Poles, Michael A., Horowitz, Amir, Kinjo, Yuki, Sullivan, Barbara, Bodmer-Narkevitch, Vera, Plettenburg, Oliver, Kronenberg, Mitchell, Tsuji, Moriya, Ho, David D., and Wong, Chi-Huey
- Published
- 2005
43. The T Cell Antigen Receptor Expressed by Vα14i NKT Cells Has a Unique Mode of Glycosphingolipid Antigen Recognition
- Author
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Sidobre, Stéphane, Bénazet-Sidobre, Lise, Maltsev, Sergei D., Richardson, Stewart K., Ndonye, Rachel M., Howell, Amy R., Sakai, Teruyuki, Besra, Gurdyal S., Porcelli, Steven A., Kronenberg, Mitchell, and Grey, Howard M.
- Published
- 2004
44. $CD4^+\>CD25^+$ T Cells Responding to Serologically Defined Autoantigens Suppress Antitumor Immune Responses
- Author
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Nishikawa, Hiroyoshi, Kato, Takuma, Tanida, Koji, Hiasa, Atsunori, Tawara, Isao, Ikeda, Hiroaki, Ikarashi, Yoshinori, Wakasugi, Hiro, Kronenberg, Mitchell, Nakayama, Toshinori, Taniguchi, Masaru, Kuribayashi, Kagemasa, Old, Lloyd J., and Shiku, Hiroshi
- Published
- 2003
45. Mouse Vα14i Natural Killer T Cells Are Resistant to Cytokine Polarization in vivo
- Author
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Matsuda, Jennifer L., Gapin, Laurent, Baron, Jody L., Sidobre, Stéphane, Stetson, Daniel B., Mohrs, Markus, Locksley, Richard M., and Kronenberg, Mitchell
- Published
- 2003
46. A crucial role for HVEM and BTLA in preventing intestinal inflammation
- Author
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Steinberg, Marcos W, Turovskaya, Olga, Shaikh, Raziya B, Kim, Gisen, McCole, Declan F, Pfeffer, Klaus, Murphy, Kenneth M, Ware, Carl F, and Kronenberg, Mitchell
- Subjects
Autoimmune Disease ,Inflammatory Bowel Disease ,Digestive Diseases ,Prevention ,Aetiology ,2.1 Biological and endogenous factors ,Animals ,B-Lymphocytes ,CD4-Positive T-Lymphocytes ,Colitis ,Homeodomain Proteins ,Inflammation ,Lymphocyte Depletion ,Mice ,Mice ,Knockout ,Receptors ,Immunologic ,Receptors ,Tumor Necrosis Factor ,Member 14 ,T-Lymphocytes ,T-Lymphocytes ,Regulatory ,Medical and Health Sciences ,Immunology - Abstract
The interaction between the tumor necrosis factor (TNF) family member LIGHT and the TNF family receptor herpes virus entry mediator (HVEM) co-stimulates T cells and promotes inflammation. However, HVEM also triggers inhibitory signals by acting as a ligand that binds to B and T lymphocyte attenuator (BTLA), an immunoglobulin super family member. The contribution of HVEM interacting with these two binding partners in inflammatory processes remains unknown. In this study, we investigated the role of HVEM in the development of colitis induced by the transfer of CD4(+)CD45RB(high) T cells into recombination activating gene (Rag)(-/-) mice. Although the absence of HVEM on the donor T cells led to a slight decrease in pathogenesis, surprisingly, the absence of HVEM in the Rag(-/-) recipients led to the opposite effect, a dramatic acceleration of intestinal inflammation. Furthermore, the critical role of HVEM in preventing colitis acceleration mainly involved HVEM expression by radioresistant cells in the Rag(-/-) recipients interacting with BTLA. Our experiments emphasize the antiinflammatory role of HVEM and the importance of HVEM expression by innate immune cells in preventing runaway inflammation in the intestine.
- Published
- 2008
47. Disruption of T Helper 2-Immune Responses in Epstein-Barr Virus-Induced Gene 3-Deficient Mice
- Author
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Neurath, Markus F., Corazza, Nadia, Iijima, Hideki, Trgovcich, Joanne, Wirtz, Stefan, Glickman, Jonathan, Bailey, Dan, Yoshida, Masaru, Galle, Peter R., Kronenberg, Mitchell, Birkenbach, Mark, and Blumberg, Richard S.
- Published
- 2002
48. Natural Killer T Cells Reactive to a Single Glycolipid Exhibit a Highly Diverse T Cell Receptor β Repertoire and Small Clone Size
- Author
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Matsuda, Jennifer L., Gapin, Laurent, Fazilleau, Nicolas, Warren, Kris, Naidenko, Olga V., and Kronenberg, Mitchell
- Published
- 2001
49. Correction to: A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers
- Author
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Rosales, Sandy Lisette, primary, Liang, Shu, additional, Engel, Isaac, additional, Schmiedel, Benjamin Joachim, additional, Kronenberg, Mitchell, additional, Vijayanand, Pandurangan, additional, and Seumois, Grégory, additional
- Published
- 2019
- Full Text
- View/download PDF
50. Presentation of Peptide Antigens by Mouse CD1 Requires Endosomal Localization and Protein Antigen Processing
- Author
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Tangri, Shabnam, Brossay, Laurent, Burdin, Nicolas, Lee, Delphine J., Corr, Maripat, and Kronenberg, Mitchell
- Published
- 1998
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