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394 results on '"Krohne, Georg"'

28. Gray platelet syndrome and defective thrombo-inflammation in Nbeal2-deficient mice

Author

Deppermann, Carsten, Cherpokova, Deya, Nurden, Paquita, Schulz, Jan-Niklas, Thielmann, Ina, Kraft, Peter, Vogtle, Timo, Kleinschnitz, Christoph, Dutting, Sebastian, Krohne, Georg, Eming, Sabine A., Nurden, Alan T., Eckes, Beate, Stoll, Guido, Stegner, David, and Nieswandt, Bernhard

Subjects
Gene expression -- Research, Blood clot -- Diagnosis -- Care and treatment -- Genetic aspects, Thrombosis -- Diagnosis -- Care and treatment -- Genetic aspects, Blood platelet disorders -- Diagnosis -- Care and treatment -- Genetic aspects, Health care industry
Abstract

Platelets are anuclear organelle-rich cell fragments derived from bone marrow megakaryocytes (MKs) that safeguard vascular integrity. The major platelet organelles, α-granules, release proteins that participate in thrombus formation and hemostasis. [...]

Published
2013
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29. Highly diverse flavobacterial phages isolated from North Sea spring blooms

Author

Bartlau, Nina, Wichels, Antje, Krohne, Georg, Adriaenssens, Evelien M., Heins, Anneke, Fuchs, Bernhard, Amann, Rudolf, Moraru, Cristina, Bartlau, Nina, Wichels, Antje, Krohne, Georg, Adriaenssens, Evelien M., Heins, Anneke, Fuchs, Bernhard, Amann, Rudolf, and Moraru, Cristina

Abstract

It is generally recognized that phages are a mortality factor for their bacterial hosts. This could be particularly true in spring phytoplankton blooms, which are known to be closely followed by a highly specialized bacterial community. We hypothesized that phages modulate these dense heterotrophic bacteria successions following phytoplankton blooms. In this study, we focused on Flavobacteriia, because they are main responders during these blooms and have an important role in the degradation of polysaccharides. A cultivation-based approach was used, obtaining 44 lytic flavobacterial phages (flavophages), representing twelve new species from two viral realms. Taxonomic analysis allowed us to delineate ten new phage genera and ten new families, from which nine and four, respectively, had no previously cultivated representatives. Genomic analysis predicted various life styles and genomic replication strategies. A likely eukaryote-associated host habitat was reflected in the gene content of some of the flavophages. Detection in cellular metagenomes and by direct-plating showed that part of these phages were actively replicating in the environment during the 2018 spring bloom. Furthermore, CRISPR/Cas spacers and re-isolation during two consecutive years suggested that, at least part of the new flavophages are stable components of the microbial community in the North Sea. Together, our results indicate that these diverse flavophages have the potential to modulate their respective host populations.

Published
2021

32. The Drosophila LEM-domain protein MAN1 antagonizes BMP signaling at the neuromuscular junction and the wing crossveins

Author

Wagner, Nicole, Weyhersmuller, Annika, Blauth, Anna, Schuhmann, Tamara, Heckmann, Manfred, Krohne, Georg, and Samakovlis, Christos

Subjects
RNA -- Physiological aspects, Drosophila -- Physiological aspects, Biological sciences
Abstract

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.ydbio.2009.11.036 Byline: Nicole Wagner (a)(b), Annika Weyhersmuller (c), Anna Blauth (d), Tamara Schuhmann (d), Manfred Heckmann (b), Georg Krohne (d), Christos Samakovlis (a) Keywords: MAN1; LEM-domain protein; Inner nuclear membrane; BMP signaling; Mad; Drosophila; Neuromuscular junction; Crossvein; Pupal wing Abstract: BMP signaling responses are refined by distinct secreted and intracellular antagonists in different cellular and temporal contexts. Here, we show that the nuclear LEM-domain protein MAN1 is a tissue-specific antagonist of BMP signaling in Drosophila. MAN1 contains two potential Mad-binding sites. We generated MAN1.sup.[DELTA]C mutants, harbouring a MAN1 protein that lacks part of the C-terminus including the RNA recognition motif, a putative Mad-binding domain. MAN1.sup.[DELTA]C mutants show wing crossvein (CV) patterning defects but no detectable alterations in nuclear morphology. MAN1.sup.[DELTA]C pupal wings display expanded phospho-Mad (pMad) accumulation and ectopic expression of the BMP-responsive gene crossveinless-2 (cv-2) indicating that MAN1 restricts BMP signaling. Conversely, MAN1 overexpression in wing imaginal discs inhibited crossvein development and BMP signaling responses. MAN1 is expressed at high levels in pupal wing veins and can be activated in intervein regions by ectopic BMP signaling. The specific upregulation of MAN1 in pupal wing veins may thus represent a negative feedback circuit that limits BMP signaling during CV formation. MAN1.sup.[DELTA]C flies also show reduced locomotor activity, and electrophysiology recordings in MAN1.sup.[DELTA]C larvae uncover a new presynaptic role of MAN1 at the neuromuscular junction (NMJ). Genetic interaction experiments suggest that MAN1 is a BMP signaling antagonist both at the NMJ and during CV formation. Author Affiliation: (a) Department of Developmental Biology, Wenner-Gren Institute, Stockholm University, S-10691 Stockholm, Sweden (b) Department of Physiology-Neurophysiology, Institute of Physiology, University of Wuerzburg, D-97070 Wuerzburg, Germany (c) Carl-Ludwig-Institute for Physiology, University Leipzig, D-04103 Leipzig, Germany (d) Division of Electron Microscopy, Biocenter of the University of Wuerzburg, D-97074 Wuerzburg, Germany Article History: Received 20 October 2009; Revised 17 November 2009; Accepted 19 November 2009

Published
2010

49. The Plastisphere – Uncovering tightly attached plastic “specific” microorganisms

Author

Kirstein, Inga, Wichels, Antje, Gullans, Elisabeth, Krohne, Georg, Gerdts, Gunnar, Kirstein, Inga, Wichels, Antje, Gullans, Elisabeth, Krohne, Georg, and Gerdts, Gunnar

Abstract

In order to understand the degradation potential of plastics in the marine environment, microorganisms that preferentially colonize and interact with plastic surfaces, as opposed to generalists potentially colonising everything, need to be identified. Accordingly, it was hypothesized that i.) plastic “specific” microorganisms are closely attached to the polymeric surface and ii.) that specificity of plastics biofilms are rather related to members of the rare biosphere. To answer these hypotheses, a three phased experiment to stepwise uncover closely attached microbes was conducted. In Phase 1, nine chemically distinct plastic films and glass were incubated in situ for 21 months in a seawater flow through system. In Phase 2, a high-pressure water jet treatment technique was used to remove the upper biofilm layers to further, in Phase 3, enrich a plastic “specific” community. To proof whether microbes colonizing different plastics are distinct from each other and from other inert hard substrates, the bacterial communities of these different substrates were analysed using 16S rRNA gene tag sequencing. Our findings indicate that tightly attached microorganisms account to the rare biosphere and suggest the presence of plastic “specific” microorganisms/assemblages which could benefit from the given plastic properties or at least grow under limited carbon resources.

Published
2019

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