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The distribution of invasive species in lower-income economies is relatively poorly understood, and many countries do not actively curate a list of species invasions or have programs to monitor or mitigate the effects of non-native species within their borders. Methods leveraging new technologies and expanding access to the internet may offer the opportunity to support low-cost and large-scale monitoring efforts. Here, we demonstrate how the use of online news coverage can support initial attempts to map the distribution of an economically-important, non-naïve freshwater fish in Bangladesh (Ptergoplichthys sp.). By initiating new collaborations between the scientific and online journalism communities, governments and organizations may be able to enhance their abilities to map the distribution of non-native species and develop new programs to support public awareness of species invasion at relatively low cost.

Anthropogenic climate change threatens the structure and function of ecosystems throughout the globe, but many people are still skeptical of its existence. Traditional “knowledge deficit model” thinking has suggested that providing the public with more facts about climate change will assuage skepticism. However, presenting evidence contrary to prior beliefs can have the opposite effect and result in a strengthening of previously held beliefs, a phenomenon known as biased assimilation or a backfire effect. Given this, strategies for effectively communicating about socioscientific issues that are politically controversial need to be thoroughly investigated. We randomly assigned 184 undergraduates from an environmental science class to one of three experimental conditions in which we exposed them to short videos that employed different messaging strategies: (a) an engaging science lecture, (b) consensus messaging, and (c) elite cues. We measured changes in student perceptions of climate change across five constructs (content knowledge, acceptance of scientific consensus, perceived risk, support for action, and climate identity) before and after viewing videos. Consensus messaging outperformed the other two conditions in increasing student acceptance of the scientific consensus, perceived risk of climate change, and climate identity, suggesting this may be an effective strategy for communicating the gravity of anthropogenic climate change. Elite cues outperformed the engaging science lecture condition in increasing student support for action on climate, with politically conservative students driving this relationship, suggesting that the messenger is more important than the message if changing opinions about the necessity of action on climate change is the desired outcome. Relative to the other conditions, the engaging science lecture did not support change in students' perceptions on climate, but appealing to student respect for authority produced positive results. Notably, we observed no decline in students' acceptance of climate science, indicating that none of the conditions induced a backfire effect., University educators are required to teach socially and politically controversial concepts to students from a wide range of political backgrounds. At the same time, university professors are increasingly more politically liberal and less religious than the general population. Therefore, it can be challenging for professors to effectively convey information about topics that are socially and politically controversial to diverse groups of students. This study sought to evaluate the effectiveness of video‐based teaching strategies to support student acceptance of global, anthropogenic climate change.

Quantifying the risk that failing onsite waste treatment systems (OWTS), such as septic systems, present to human health and the environment is a key component in natural resource management. We integrated environmental and socio-demographic data to assess the potential environmental risk and environmental justice concerns related to septic infrastructure. We used this process to develop a framework that can be applied in other jurisdictions. We found only 8% of the registered OWTS presented potential environmental risk due to the topographic, hydrologic, or edaphic characteristics of their placement. In contrast, almost 70% of the OWTS presented potential environmental risk due to their age (25 years or older). Approximately 60% of the OWTS we estimated to be at risk from age or placement were found in census blocks with more than 30% of the population living below the poverty line, had a population that was more than 50% nonwhite, or was predominantly nonwhite and impoverished. Our work suggests that jurisdictions with limited information about septic infrastructure may be able to use geospatial data that they do have to predict the parcel-level locations of OWTS. These locations can then be used to inform environmental monitoring to proactively address environmental justice concerns.

Microbes play a critical role in plant litter decomposition and influence the fate of carbon in rivers and riparian zones. When decomposing low-nutrient plant litter, microbes acquire nitrogen (N) and phosphorus (P) from the environment (i.e., nutrient immobilization), and this process is potentially sensitive to nutrient loading and changing climate. Nonetheless, environmental controls on immobilization are poorly understood because rates are also influenced by plant litter chemistry, which is coupled to the same environmental factors. Here we used a standardized, low-nutrient organic matter substrate (cotton strips) to quantify nutrient immobilization at 100 paired stream and riparian sites representing 11 biomes worldwide. Immobilization rates varied by three orders of magnitude, were greater in rivers than riparian zones, and were strongly correlated to decomposition rates. In rivers, P immobilization rates were controlled by surface water phosphate concentrations, but N immobilization rates were not related to inorganic N. The N:P of immobilized nutrients was tightly constrained to a molar ratio of 10:1 despite wide variation in surface water N:P. Immobilization rates were temperature-dependent in riparian zones but not related to temperature in rivers. However, in rivers nutrient supply ultimately controlled whether microbes could achieve the maximum expected decomposition rate at a given temperature. Collectively, we demonstrated that exogenous nutrient supply and immobilization are critical control points for decomposition of organic matter. Key Points • Nitrogen (N) and phosphorus (P) immobilization was measured on organic matter (cotton) in 100 rivers and riparian zones representing 11 biomes • Elevated temperature in riparian zones and phosphate in rivers increased immobilization, and consequently accelerated decomposition • N and P immobilization was strongly linked by microbial stoichiometry despite widely varied surface-water nutrient ratios Plain Language Summary Bacteria and fungi contribute to the breakdown of leaf litter in rivers and floodplains. To break down leaf litter, these microbes need the nutrients nitrogen and phosphorus (P), and microbes can get nutrients either from the leaf litter itself or from the environment. Most leaf litter has low nutrient content and microbes must rely on the environment to supply nutrients. We studied microbial nutrient uptake from the environment during litter breakdown to determine whether it varies predictably across the globe and how it is influenced by changing climate and nutrient pollution. In 100 rivers and floodplains in 11 of Earth's major biomes we placed small strips of cotton as stand-ins for leaf litter. Nutrient uptake was consistently greater on cotton strips that were submerged in the river compared to cotton on the floodplain. For microbes in the river, nutrient uptake was faster in instances where there was more P in the water. For microbes in the floodplain, nutrient uptake was faster where temperatures were warmer. Faster nutrient uptake by microbes was linked with faster cotton breakdown in rivers and floodplains. Our study shows that climate change and nutrient pollution can alter the activity of microbes in rivers and floodplains.

Antibiotic resistance is a global threat to human health. Many surface water resources are environmental hotspots of antibiotic resistant gene (ARG) transfer, with agricultural runoff and human waste highlighted as common sources of ARGs to aquatic systems. Here we quantified fecal marker genes and ARGs in 992 stream water samples collected seasonally during a 5-year period from 115 sites across the Upper Oconee watershed (Georgia, USA), an area characterized by gradients of agricultural and urban development. Widespread fecal contamination was found from humans (48% of samples), ruminants (55%), and poultry (19%), and 73% of samples tested positive for at least one of the six targeted ARGs (ermB, tet(B), bla

Ecological stoichiometry uses information on a few key biological elements (C, N, and P) to explain complex ecological patterns. Although factors driving variation in these elements are well-established, expanding stoichiometric principles to explore dynamics of the many other essential elements comprising biological tissues (i.e., the ionome) is needed to determine their metabolic relationships and better understand biological control of elemental flows through ecosystems. 2. In this paper, we report observations of ionomic variation in two species of salamander (Ambystoma opacum and A. talpoideum) across ontogenic stages using specimens from biological collections of two wetlands sampled over a 30-year period. This unique data set allowed us to explore the extent of ionomic variation between species, among ontogenic stages, between sites, and through time. 3. We found species- and to a lesser extent site-specific differences in C, N, and P along with 13 other elements forming salamander ionomes but saw no evidence of temporal changes. Salamander ionomic composition was most strongly related to ontogeny with relatively higher concentrations of many elements in adult males (i.e., Ca, P, S, Mg, Zn, and Cu) compared to metamorphic juveniles, which had greater amounts of C, Fe, and Mn. 4. In addition to patterns of individual elements, covariance among elements was used to construct multi-elemental nutrient balances, which revealed differences in salamander elemental composition between species and sites and changes in elemental proportions across ontogenic development. These multi-elemental balances distinguished among species-site-ontogenic stage groups better than using only C, N, and P. 5. Overall, this study highlights the responsiveness of consumer ionomes to life-history and environmental variation while reflecting underlying relationships among elements tied to biological function. As such, ionomic studies can provide important insights into factors shaping consumer elemental composition and for predicting how these changes might affect higher-order ecological processes.

Microbes include heterotrophs, especially bacteria and fungi that obtain energy from detrital material as well as from organic compounds in the surrounding water, and some autotrophs. They occur on virtually all surfaces in streams as biofilms that include bacteria, fungi, algae, protists, detrital particles, various exudates, exoenzymes, and metabolic products in an organic microlayer. Biofilms are important sites of organic matter processing and nutrient cycling. Organic molecules exuded by actively growing algae can be used by microbes, while microbial decomposition of non-living organic matter releases inorganic compounds beneficial to algal production. Microbial production forms the base of many stream food webs, and the flow of carbon and elements through microbial communities and into higher trophic levels involves complex linkages. Fungi and bacteria aid in the breakdown of organic matter such as autumn-shed leaves, making this energy source accessible to higher trophic levels. The microbial tissue in biofilms is available to a wide range of micro-consumers, including protozoans and small metazoans termed meiofauna. Still relatively under-studied, a food web involving meiofauna exists within biofilms and in plankton assemblages, possibly serving as a link to higher trophic levels, and possibly dissipating most of the energy available within a “microbial loop”.

Organic carbon is the fundamental currency of energy transfer in food webs. The study of stream ecosystem metabolism integrates all energy producing and energy consuming processes, providing a whole-ecosystem perspective of lotic ecosystems. The focus is on two central questions. First, what are the relative magnitudes of internal versus external energy sources, including their variation along a river’s length and with landscape setting? Second, how efficient is the stream ecosystem in metabolizing those energy supplies, versus export to downstream ecosystems and possibly to the oceans? The first is addressed by comparing gross primary production to ecosystem respiration. Mass balance estimation of all inputs and exports, and measures of the efficiency with which organic carbon is utilized, addresses the second question. The relationship between gross primary production and ecosystem respiration can indicate whether an ecosystem is reliant on internal production or requires external subsidies of organic matter to sustain whole-system respiration. Wherever gross primary production is low relative to ecosystem respiration, that location clearly is dependent upon external energy inputs, either from the adjacent terrestrial ecosystem or from upstream sources. Ecosystems where productivity exceeds respiration are likely to export organic matter downstream, and this is often the case for low-order streams.

The world’s ecosystems have been extensively altered throughout the age of the Anthropocene, river ecosystems perhaps most of all.

The diversity of running waters is enormous, from cascading mountain streams, to meandering lowland rivers, to great rivers that occupy sub-continents. Here we set the stage for the rest of this book: first, we describe key elements of a healthy river; second, we review the many threats that rivers face; lastly, we provide an outline of forthcoming chapters as they build toward an understanding of the ecological, biogeochemical, hydrologic, and geomorphologic processes that govern the structure and function of river systems. Today, human impacts on the Earth are so profound that we have entered a new geologic epoch, the Anthropocene. Dams now influence nearly half of global river volume, and their impoundments trap one-fourth of the global sediment load before it reaches the oceans. Freshwater biodiversity is in serious decline, and many rivers can no longer be considered in good condition. To ensure healthy rivers for future generations, we must understand the fundamental principles of river science. Our ability to improve, restore, and protect river ecosystems rests on a full understanding of how these ecosystems change in response to human pressures, and of the tools that are available to us to prevent and reverse harm.

Food webs describe the network of linkages that are responsible for transfers of energy among species, and for the movement of nutrients between organisms and the abiotic environment. Food web networks ideally describe all interactions within a community of species, while recognizing that much of the energy flows through relatively few species. These pathways vary along a river’s length, with season, and among ecosystems. Food webs often receive external inputs, including allochthonous leaf litter, invertebrates from the terrestrial riparian zone, and organic matter transported from upstream, termed spatial subsidies. In turn, riverine ecosystems export material downstream, and the adults of aquatic invertebrates subsidize food webs along the river’s banks. Functional classifications of species’ roles in nutrient and energy flows can be based on a variety of species attributes. Often, organisms will have complementary or overlapping roles for a given functional attribute, but at least in some cases species appear to be functionally irreplaceable. Animals consume and store resources in body tissues, and subsequently mineralize elements through excretion, egestion, and death and decomposition. In this manner they play a critical role in biogeochemical cycling, referred to as consumer-driven nutrient dynamics.

Current, substrate, and temperature strongly influence species occurrence and abundance for the denizens of running waters. Current governs the physical form of habitat and affects organisms through drag and dislodgment, but also delivers food and nutrients while removing wastes. A variety of sophisticated methods measure current and associated hydraulic forces on substrate and organisms, but it remains challenging to quantify the forces exerted on small organisms residing on substrates, due to both scale and turbulence. Substrate encompasses a wide variety of inorganic and organic material from fine silt to boulders and large wood, making it challenging to quantify. Because most stream-dwelling organisms are ectotherms, temperature influences their metabolic rates, distributions, and quite possibly their success in interacting with other species. Stream temperature can vary on seasonal and daily time scales and among locations due to climate, extent of streamside vegetation, and the relative importance of groundwater inputs. Human activities alter the natural temperature regime by removal of shade, changes to flow paths such as increased impervious surface, construction of impoundments, and by influencing the climate. Stream restoration commonly requires methods to reverse habitat degradation due to human-caused changes in these features of the abiotic environment.

International audience; In the Fall of 2016 a workshop was held which brought together over 50 scientists from the ecological and radio- logical fields to discuss feasibility and challenges of reintegrating ecosystem science into radioecology. There is a growing desire to incorporate attributes of ecosystem science into radiological risk assessment and radioecological research more generally, fueled by recent advances in quantification of emergent ecosystem at- tributes and the desire to accurately reflect impacts of radiological stressors upon ecosystem function. This paper is a synthesis of the discussions and consensus of the workshop participant's responses to three primary questions, which were: 1) How can ecosystem science support radiological risk assessment? 2) What ecosystem level endpoints potentially could be used for radiological risk assessment? and 3) What inference strategies and associated methods would be most appropriate to assess the effects of radionuclides on ecosystem structure and function? The consensus of the participants was that ecosystem science can and should support radiological risk assessment through the incorporation of quantitative metrics that reflect ecosystem functions which are sensi- tive to radiological contaminants. The participants also agreed that many such endpoints exit or are thought to exit and while many are used in ecological risk assessment currently, additional data need to be collected that link the causal mechanisms of radiological exposure to these endpoints. Finally, the participants agreed that ra- diological risk assessments must be designed and informed by rigorous statistical frameworks capable of reveal- ing the causal inference tying radiological exposure to the endpoints selected for measurement.

Land use change threatens the ecological integrity of tropical rivers and streams; however, few studies have simultaneously analyzed the taxonomic and functional responses of tropical macroinvertebrates to riparian forest conversion. Here, we used community structure, functional diversity, and stable isotope analyses to assess the impacts of riparian deforestation on macroinvertebrate communities of streams in southern Mexico. Monthly sampling during the dry season was conducted in streams with riparian forest (forest streams), and in streams with pasture dominating the riparian vegetation (pasture streams). Samples were collected for water quality (physical-chemical variables, nutrient concentrations, and total suspended solids), organic matter (leaf litter abundance and algal biomass), and macroinvertebrate abundance and diversity. Higher temperature, conductivity, suspended solids, and chlorophyll a were detected in pasture streams, while nitrate concentrations and leaf litter biomass were greater in forest streams. Macroinvertebrate density was higher in pasture sites, while no differences in taxonomic diversity and richness were found between land uses. Functional evenness was greater in forest streams, while richness and divergence were similar between land uses, despite differences in taxonomic composition. Environmental variables were associated with taxa distribution but not with functional traits, suggesting current conditions still promote redundancy in ecological function. Isotopic analyses indicated consumers in pasture streams were enriched in 13C and 15N relative to forest streams, potentially reflecting the higher algal biomass documented in pasture systems. Isotopic niches were broader and more overlapped in pasture streams, indicating more generalist feeding habits. No significant losses of taxonomic or functional diversity were detected in pasture streams. However, changes in trophic ecology suggest landscape-level processes are altering macroinvertebrate feeding habits in streams. The changes we observed in habitat, water quality, and macroinvertebrate community were related to the removal of the riparian vegetation, suggesting the structure and function of the focal systems would benefit from riparian restoration.

Identifying freshwater systems that are at risk from anthropogenic stressors is a pressing management problem. In particular, the detection of metal pollution is often constrained by data availability and resources. To address this challenge and develop a tool to identify susceptible systems, we tested whether land cover could be predictive of stream sensitivity to metal pollution, as determined by the biotic ligand model (BLM). We used water chemistry data from the conterminous United States to estimate metal sensitivity in streams using two BLMs (i.e., HydoQual, Bio-Met). Subsequently, we combined the sensitivity estimates with land cover and physiochemical data from the GAGES-II database to build predictive models of sensitivity to metals in streams. When combined, our predictor variables (e.g., land cover, mean annual temperature, mean annual precipitation) generally explained about half of the variation in our dataset. In each model, the percent of wetlands in a watershed was strongly correlated with reduced sensitivity to metals, likely due to increased concentrations of dissolved organic carbon associated with wetlands. To validate the utility of the models, we used them to predict metal sensitivity in sites where metal concentrations had been collected, but where the full suite of BLM parameters were unknown. We were able to classify several hundred sites which are likely at risk to metal pollution. Our work highlights the value in considering metal toxicity at the landscape-scale and describes a new approach to estimate metal sensitivity when site-specific chemical parameters are unknown.

Dam construction and nutrient loading are among the greatest threats to freshwater ecosystems, altering ecological processes and the provisioning of ecosystem services. Temporal change in hydrology and ambient nitrogen and phosphorus concentrations was studied on the Grijalva, a large tropical river in southern Mexico, where four hydroelectric dams operate and where land conversion has impacted the freshwater environment. Temporal changes in discharge and in river chemistry were examined by analysing long‐term discharge and nutrient data using the software Indicators of Hydrologic Alteration and Mann–Kendall tests. Furthermore, additional water chemistry samples were collected to examine seasonal nutrient dynamics in the lower Grijalva. Long‐term discharge data indicated dam construction has severely altered temporal patterns in discharge and other hydrological characteristics. The lower Grijalva has also experienced increase in nitrate concentrations through time, which may be attributed to the expansion of agricultural and urban areas in the watershed. In contrast, total phosphorus appeared to decline at the sites influenced by dam construction. Lower nutrient concentrations were recorded upstream from the city of Villahermosa, suggesting that inputs from urban areas may have contributed to nutrient loading. Additionally, higher nitrate and total phosphorus concentrations were detected in tributaries draining intensive agricultural and suburban areas. Collectively, the results from the study suggest that dam construction and land conversion in large, tropical watersheds can produce chemical and hydrological changes, which may negatively impact important ecosystem services—such as fisheries and the provisioning of sources of drinking water—and may compromise the integrity of coastal zones.

As human population densities grow around the boundaries of protected areas in lower-income economies, there are frequently concomitant increases in environmental degradation. The purpose of this study was to examine water chemistry and the isotopic signatures of primary consumers in streams in and around a national park in the tropics to document whether park watersheds were affected by untreated wastewater. Three of the six study sites had concentrations of soluble reactive phosphorus >39 μg/L. Additionally, there was a strong, positive correlation between δN15 values of grazing snails and phosphorus concentrations of the water, suggesting that sewage effluent is influencing trophic ecology in the protected aquatic habitats. The results from this study lend support to recent calls for management beyond riparian buffers for conservation of freshwater ecosystem integrity in protected areas.

The role of animals in modulating nutrient cycling [hereafter, consumer-driven nutrient dynamics (CND)] has been accepted as an important influence on both community structure and ecosystem function in aquatic systems. Yet there is great variability in the influence of CND across species and ecosystems, and the causes of this variation are not well understood. Here, we review and synthesize the mechanisms behind CND in fresh waters. We reviewed 131 articles on CND published between 1973 and 1 June 2015. The rate of new publications in CND has increased from 1.4 papers per year during 1973–2002 to 7.3 per year during 2003–2015. The majority of investigations are in North America with many concentrating on fish. More recent studies have focused on animal-mediated nutrient excretion rates relative to nutrient demand and indirect impacts (e.g. decomposition). We identified several mechanisms that influence CND across levels of biological organization. Factors affecting the stoichiometric plasticity of consumers, including body size, feeding history and ontogeny, play an important role in determining the impact of individual consumers on nutrient dynamics and underlie the stoichiometry of CND across time and space. The abiotic characteristics of an ecosystem affect the net impact of consumers on ecosystem processes by influencing consumer metabolic processes (e.g. consumption and excretion/egestion rates), non-CND supply of nutrients and ecosystem nutrient demand. Furthermore, the transformation and transport of elements by populations and communities of consumers also influences the flow of energy and nutrients across ecosystem boundaries. This review highlights that shifts in community composition or biomass of consumers and eco-evolutionary underpinnings can have strong effects on the functional role of consumers in ecosystem processes, yet these are relatively unexplored aspects of CND. Future research should evaluate the value of using species traits and abiotic conditions to predict and understand the effects of consumers on ecosystem-level nutrient dynamics across temporal and spatial scales. Moreover, new work in CND should strive to integrate knowledge from disparate fields of ecology and environmental science, such as physiology and ecosystem ecology, to develop a comprehensive and mechanistic understanding of the functional role of consumers. Comparative and experimental studies that develop testable hypotheses to challenge the current assumptions of CND, including consumer stoichiometric homeostasis, are needed to assess the significance of CND among species and across freshwater ecosystems.

Extreme weather events, such as hurricanes, can cause ecological disturbances that alter energy and nutrients across terrestrial-aquatic boundaries. Yet, relatively few studies have considered the impacts of extreme weather events on biogeochemical dynamics in watersheds at larger spatial scales. Here, we assessed the effects of Hurricanes Harvey and Irma on the export of dissolved organic matter (DOM) and nutrients in ten watersheds from five southeastern states of the United States. We quantified the magnitude of dissolved organic carbon (DOC) and nutrients exported during the storms and assessed the changes in DOM sources and bioreactivity after storms. Our results show that the storm-mobilized DOC and nutrients fluxes were primarily driven by water discharge. The proportions of terrestrial, humic-like DOM compounds increased, and percent autochthonous, protein-like DOM decreased during high flows. Percent bioreactive DOC decreased with increasing discharge. Bioreactivity increased with increasing nitrate concentration, but decreased as percent terrestrial humic-like DOM, aromaticity, and molecular weight increased. These observations suggest that storms may have shifted flow paths to shallower depths that promoted the addition of biorefractory organic matter from topsoils into the water column. Notably, the total flux of bioreactive DOC was at least nearly twice as high at peak discharge, indicating materials transported by large storm flows could strongly enhance microbial activity in streams, although the position of storm-mediated microbial hotspots would depend on the flow rate and other instream parameters. Additionally, compared to forest-dominated watersheds, urban watersheds exported high loads of nutrients and bioreactive DOC, and a wetland-dominated watershed had a prolonged, but relatively subdued export of DOC and nutrients. Together, our findings highlight the ecological significance of extreme weather and climate events in leading to rapid, large-magnitude changes in energy and nutrient availability within drainage networks, and the potential interactions between land use and climate change on watershed biogeochemistry.

Urban streams commonly express degraded physical, chemical, and biological conditions that have been collectively termed the “urban stream syndrome”. The description of the syndrome highlights the broad similarities among these streams relative to their less-impaired counterparts. Awareness of these commonalities has fostered rapid improvements in the management of urban stormwater for the protection of downstream watercourses, but the focus on the similarities among urban streams has obscured meaningful differences among them. Key drivers of stream responses to urbanization can vary greatly among climatological and physiographic regions of the globe, and the differences can be manifested in individual stream channels even through the homogenizing veneer of urban development. We provide examples of differences in natural hydrologic and geologic settings (within similar regions) that can result in different mechanisms of stream ecosystem response to urbanization and, as such, should lead to differe...

Urbanization is occurring at a rapid pace in developing countries. The urban stream syndrome has been well documented in higher-income countries, but in lower-income, developing countries, resources often are unavailable for quantifying how urbanization affects streams. Basic infrastructure to support water supply and wastewater treatment frequently is lacking in lower-income countries, and this situation has repercussions for human health and for ecosystem structure and function. The interaction of environmental, social, and economic factors may produce differences in the expression of the urban stream syndrome in lower-income countries relative to in high-income countries. We address how patterns of economic development and urbanization can influence the quality of freshwater resources, and we discuss some of the relationships between urban watersheds and marginalized human populations in lower-income countries. We argue that sustainable management of urban watersheds and the provisioning of dri...

The field of urban stream ecology has evolved rapidly in the last 3 decades, and it now includes natural scientists from numerous disciplines working with social scientists, landscape planners and designers, and land and water managers to address complex, socioecological problems that have manifested in urban landscapes. Over the last decade, stream ecologists have met 3 times at the Symposium on Urbanization and Stream Ecology (SUSE) to discuss current research, identify knowledge gaps, and promote future research collaborations. The papers in this special series on urbanization and stream ecology include both primary research studies and conceptual synthesis papers spurred from discussions at SUSE in May 2014. The themes of the meeting are reflected in the papers in this series emphasizing global differences in mechanisms and responses of stream ecosystems to urbanization and management solutions in diverse urban streams. Our hope is that this series will encourage continued interdisciplinary an...

Metal accumulation in aquatic food webs is mediated by physiochemical parameters of the environment and organismal traits. Trophic strategies influence an organisms’ exposure to metal pollution, but links between trophic ecology and exposure to divalent metals are relatively understudied. While organically bound metals are typically considered unavailable for uptake, organisms directly consuming dissolved organic carbon (DOC) and bacteria—via the microbial loop—must also be consuming organically bound metals. Hence, we predicted animals feeding within the microbial loop would accumulate metals through their diet. To test this prediction, we exploited dietary differences between two organisms, Simulium vittatum, a filter-feeding black fly and Hyalella azteca, a shredding detritivore. We exposed both species to three treatments of DOC (labile, recalcitrant, and no additional DOC) that were crossed with exposure to variable copper (Cu) concentrations (2–14 μg L–1) in laboratory microcosms. As predicted, H. a...

Animals mediate flows of elements and energy in ecosystems through processes such as nutrient sequestration in body tissues, and mineralization through excretion. For taxa with biphasic life cycles, the dramatic shifts in anatomy and physiology that occur during ontogeny are expected to be accompanied by changes in body and excreta stoichiometry, but remain little-explored, especially in vertebrates. Here we tested stoichiometric hypotheses related to the bodies and excreta of the wood frog (Lithobates sylvaticus) across life stages and during larval development. Per-capita rates of nitrogen (N) and phosphorus (P) excretion varied widely during larval ontogeny, followed unimodal patterns, and peaked midway through development (Taylor-Kollros stages XV and XII, respectively). Larval mass did not increase steadily during development but peaked at stage XVII and declined until the termination of the experiment at stage XXII. Mass-specific N and P excretion rates of the larvae decreased exponentially during development. When coupled with population-biomass estimates, population-level excretion rates were greatest at stages VIII-X. Percent carbon (C), N, and C:N of body tissue showed weak trends across major life stages; body P and C:P, however, increased sixfold during development from egg to adult. Our results demonstrate that intraspecific ontogenic changes in nutrient contents of excretion and body tissues can be significant, and that N and P are not always excreted proportionally throughout life cycles. These results highlight the dynamic roles that species play in ecosystems, and how the morphological and physiological changes that accompany ontogeny can influence ecosystem-level processes.

Low-trophic-position animals, such as herbivorous and detritivorous fishes, mollusks, and crustaceans, have been introduced and become established in aquatic ecosystems throughout the globe. After introduction, they have the potential to change community structure and ecosystem processes fundamentally. Armored catfishes (Siluriformes:Loricariidae) are grazing fishes that have an extensive nonnative distribution. High population densities of nonnative loricariids have been linked to the decline of freshwater fisheries in invaded habitats, but the mechanisms underlying declines are poorly understood. We coupled the results of 2 loricariid exclosure experiments with a comparison of invaded and uninvaded sites to measure the effects of loricariids on the quantity and quality of food resources in a tropical stream. Loricariid grazing reduced the standing stock of benthic organic matter and algal biomass in experimental manipulations and in the site comparison. Moreover, grazing by loricariids significa...

In terrestrial and aquatic ecosystems, organisms directly affect nutrient storage and cycling by sequestering nutrients via growth and remineralizing nutrients via excretion and egestion. Therefore, species introductions and extirpations can profoundly affect nutrient storage and remineralization rates, and present a challenge for conserving ecosystem function in fresh waters. The literature of consumer-driven nutrient dynamics is growing rapidly, but studies of consumer effects on nutrient storage and remineralization across species and among ecosystems are limited. We compared the effects of 3 grazing taxa, nonnative armored catfish in Mexican streams, native mussels in Oklahoma streams, and native tadpoles in Panamanian streams, on nutrient storage and remineralization. We examined interactions among organismal stoichiometry and biomass, nutrient storage, remineralization rates, and ecosystem size across these 3 groups following species decline (tadpoles and mussels) or introduction (armored ...