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Asymmetries in brain and behaviour have been demonstrated in numerous species representing all major vertebrate taxa, and may be a universal feature of the vertebrate nervous system. While descriptions of lateralization at the behavioural and neuroanatomical level are widespread, examples of correlation between asymmetries in behaviour and neural structures remain relatively scarce. In the past few years, the habenular nucleus has emerged as a potential site for the neural basis of some lateralized behaviours. Here we investigate the relation between continuous individual variation in asymmetry of the habenulae and behaviour in the detour task in the convict cichlid (Amatitlania nigrofasciata). We found that both male and female convicts show a significant population-level bias towards relatively larger left habenulae. We also show that habenular asymmetry is correlated with behavioural lateralization in both males and females, but in opposite directions. This adds to previous studies showing both in convict cichlids and other vertebrates an interaction between sex and lateralized behaviour. The results of this study increase our understanding of the role of the habenula in lateralized behaviour and highlight the importance of a comparative approach to understanding the development and evolution of habenular asymmetry., (Copyright © 2011 Elsevier B.V. All rights reserved.)

The cerebellar cortex has a fundamental parasagittal organization that is reflected in the physiological responses of Purkinje cells, afferent and efferent connections, and the expression of several molecular markers. The most thoroughly studied of these molecular markers is zebrin II (ZII; a.k.a. aldolase C). ZII is differentially expressed in Purkinje cells, resulting in a pattern of sagittal stripes of high expression interdigitated with stripes of little or no expression. In this study, we examined the expression of the calcium binding protein parvalbumin (PV) in the cerebellum of several avian species (pigeons, hummingbirds, zebra finches) and compared it to the expression of ZII. We found that PV immunoreactivity was distributed across the cerebellar cortex such that there were sagittal stripes of PV immunopositive (PV+) Purkinje cells alternating with PV immunonegative (PV-) Purkinje cells. Although most Purkinje cells in the anterior lobe were PV+, there were several thin (i.e. only a few Purkinje cells wide) PV- stripes spanning the folia. In the posterior lobe, PV+ and PV- stripes were also apparent, but the PV- stripes were much wider than in the anterior lobe. In sections processed for both ZII and PV, the expression was generally complementary: PV+ stripes were ZII-, and vice-versa. This complementary expression was most apparent in folia II-IV and VIII-IXcd. The complementary expression was not, however, absolute; some Purkinje cells co-expressed PV and ZII whereas others lacked both. These novel findings relate to the complex neurochemical organization of the cerebellum, and are likely important to issues regarding cerebellar plasticity., (Copyright © 2011 IBRO. Published by Elsevier Ltd. All rights reserved.)