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191 results on '"Kremsdorf D"'

9. Alternative splicing regulation during the course of liver disease

Author

Wang, H., primary, Lekbaby, B., additional, Fares, N., additional, Attout, T., additional, Schnuriger, A., additional, Doulcier, A.-M.C., additional, Panasyuk, G., additional, Augustin, J., additional, Perlemuter, G., additional, Bieche, I., additional, Vacher, S., additional, Hall, J., additional, Merle, P., additional, Kremsdorf, D., additional, Chemin, I., additional, and Soussan, P., additional

Published
2018
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11. Modeling HBV infections and immune responses in humanized mouse models

Author

Dusseaux, M., primary, Masse-Ranson, G., additional, Darche, S., additional, Ahodantin, J., additional, Li, Y., additional, Beaumont, E., additional, Moreau, P., additional, Neuveut, C., additional, Soussan, P., additional, Roingeard, P., additional, Kremsdorf, D., additional, Santo, J.D., additional, and Strick-Marchand, H., additional

Published
2018
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16. Liver-stage development of Plasmodium falciparum, in a humanized mouse model.

Author

Morosan, S., Hez-Deroubaix, S., Verduyn Lunel, F.M., Renia, L., Giannini, C., Rooijen, N. van, Battaglia, S., Blanc, C., Eling, W.M.C., Sauerwein, R.W., Hannoun, L., Belghiti, J., Brechot, C., Kremsdorf, D., Druilhe, P., Morosan, S., Hez-Deroubaix, S., Verduyn Lunel, F.M., Renia, L., Giannini, C., Rooijen, N. van, Battaglia, S., Blanc, C., Eling, W.M.C., Sauerwein, R.W., Hannoun, L., Belghiti, J., Brechot, C., Kremsdorf, D., and Druilhe, P.

Abstract

Item does not contain fulltext, BACKGROUND: The liver stage of the human malaria parasite Plasmodium falciparum is the least known, yet it holds the greatest promise for the induction of sterile immunity and the development of novel drugs. Progress has been severely limited by the lack of adequate in vitro and in vivo models. METHODS: Recently, it was found that immunodeficient mice transgenic for the urokinase plasminogen activator allow survival of differentiated human hepatocytes. We confirm this finding but show that hepatocyte survival is short lived unless nonadaptive defenses are simultaneously depleted. RESULTS: By controlling macrophages and NK cells, we readily effected the long-term secretion of human serum albumin and human alpha-1 antitrypsin in mouse serum (at 3 months, the proportion of repopulated mice increased from 0% to 60% and from 22% to 80%, respectively; P<.0001). P. falciparum sporozoites delivered intravenously into mice readily infected transplanted human hepatocytes and developed into liver schizonts. Their size was twice as large as what was seen in vitro and was comparable to that found in humans and chimpanzees. CONCLUSION: These results emphasize the importance of nonadaptive defenses against xenotransplantation and lead to development of small laboratory models that, because they can harbor human hepatocytes, provide novel opportunities to study intrahepatic pathogens, such as those causing malaria and hepatitis.

Published
2006

19. 732 PROPORTION OF DEFECTIVE HBV DNA ISSUED FROM A SINGLY SPLICED RNA AND ENCODING FOR HBSP IS RELATED TO THE COURSE OF VIRAL INFECTION

Author

Redelsperger, F., primary, Lekbaby, B., additional, Désiré, N., additional, Roque-Afonso, A.M., additional, Brichler, S., additional, Perlemuter, G., additional, Dubreuil, P., additional, Bacon, N., additional, Zatla, F., additional, Le Pendeven, C., additional, Kremsdorf, D., additional, and Soussan, P., additional

Published
2010
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46. Human papillomaviruses associated with epidermodysplasia verruciformis. II. Molecular cloning and biochemical characterization of human papillomavirus 3a, 8, 10, and 12 genomes

Author

Kremsdorf, D, Jablonska, S, Favre, M, and Orth, G

Abstract

The DNAs of four human papillomaviruses (HPVs) that were found in the benign lesions of three patients suffering from epidermodysplasia verruciformis have been characterized. The flat wart-like lesions and the macular lesions of patient 1 contained two viruses, HPV-3a and HPV-8, respectively, whose genomes had previously been only partially characterized. The flat wart-like lesions of patient 2 and the macular lesions of patient 3 each contained a virus previously considered as belonging to types 3 and 5, respectively. These viruses are shown in the present study to be different from all of the HPV types so far characterized; they have tentatively been named HPV-10 and HPV-12. The HPV-3a, HPV-8, and HPV-12 DNAs and the two SalI fragments of HPV-10 DNA (94.1 and 5.9% of the genome length) were cloned in Escherichia coli after having been inserted in plasmid pBR322. The cloned HPV genomes have similar sizes (about 7,700 base pairs), but their guanine-plus-cytosine contents differ from 41.8% for HPV-12 DNA to 45.5% for HPV-3a DNA. The study of the sensitivity of the four HPV DNAs to 14 restriction endonucleases permitted the construction of cleavage maps. Evidence for conserved restriction sites was found only for the HPV-3a and HPV-10 genomes since 5 of the 21 restriction sites localized in the HPV-3a DNA seem to be present also in the HPV-10 DNA. Hybridization experiments, performed in liquid phase at saturation, showed a 35% sequence homology between HPV-3a and HPV-10 DNAs, 17 to 29% sequence homology among HPV-5, HPV-8, and HPV-12 DNAs, almost no sequence homology between the HPV-3a or HPV-10 DNA and the other HPV DNAs, and a weak homology between HPV-9 DNA and HPV-8 or HPV-12 DNA. Blot hybridization experiments showed no sequence homology between the HPV-3a, HPV-8, and HPV-12 DNAs and the DNAs of the HPVs associated with skin warts (HPV-1a, HPV-2, HPV-4, and HPV-7) or with mucocutaneous and mucous membrane lesions (HPV-6b and HPV-11a, respectively). One exception was a weak sequence homology between the HPV-2 prototype and HPV-3a or HPV-10 DNA.(ABSTRACT TRUNCATED AT 400 WORDS)

Published
1983
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47. Biochemical characterization of two types of human papillomaviruses associated with epidermodysplasia verruciformis

Author

Kremsdorf, D, Jablonska, S, Favre, M, and Orth, G

Abstract

The DNAs of the human papillomaviruses (HPVs) associated with the benign lesions of two patients suffering from epidermodysplasia verruciformis (patients JD and JK) were analyzed by using 12 restriction endonucleases. None of the restriction endonucleases were one-cut enzymes for the HPV DNA obtained from patient JD, referred to as the prototypical HPV-5, whereas five of them were one-cut enzymes for the DNA of the major virus found in patient JK, referred to as HPV-9. The molecular cloning of the two fragments resulting from the cleavage of HPV-5 DNA by endonuclease HindIII and of the single fragment obtained after treatment of HPV-9 DNA with endonuclease BamHI was performed in Escherichia coli after the fragments were inserted in plasmid pBR322. A cleavage map of the two cloned genomes was constructed. Little sequence homology (4 to 5%) was detected between HPV-5 and HPV-9 DNAs by DNA-DNA hybridization experiments in liquid phase at saturation; this homology was reproducibly higher than that (2 to 3%) detected under the same conditions between these DNAs and HPV-1a DNA. In addition, blot hybridization experiments performed under stringent conditions showed no or little sequence homology between the DNAs of HPV-5 and HPV-9 and those of HPV prototypes of types 1, 2, 3, 4, and 7 associated with skin warts. These results confirm that HPV-5 and HPV-9 are two distinct HPV types.

Published
1982
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48. Molecular cloning and characterization of the genomes of nine newly recognized human papillomavirus types associated with epidermodysplasia verruciformis

Author

Kremsdorf, D, Favre, M, Jablonska, S, Obalek, S, Rueda, L A, Lutzner, M A, Blanchet-Bardon, C, Van Voorst Vader, P C, and Orth, G

Abstract

The genomes of 11 human papillomaviruses (HPVs) found in benign lesions of eight patients suffering from epidermodysplasia verruciformis were cloned in Escherichia coli after insertion into plasmid pBR322. The study of the sensitivity of the cloned HPV DNAs to 14 restriction endonucleases permitted the construction of physical maps. DNA-DNA hybridization experiments, performed under stringent conditions, showed that these viruses represent nine new types, HPVs 14 (with subtypes a and b), 15, 17 (with subtypes a and b), 19, 20, 21, 22, 23, and 24. These HPVs were divided into three groups based on an absent or very weak cross-hybridization among the genomes of the viruses belonging to different groups.

Published
1984
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