Search

Your search keyword '"Kraková, Lucia"' showing total 152 results
Start Over Author "Kraková, Lucia"
152 results on '"Kraková, Lucia"'

5. Evaluation of Microbial Degradation of Thermoplastic and Thermosetting Polymers by Environmental Isolates.

Author

Nuccetelli, Pierluca, Maisto, Francesca, Kraková, Lucia, Grilli, Alfredo, Takáčová, Alžbeta, Šišková, Alena Opálková, and Pangallo, Domenico

Subjects
THERMOSETTING polymers, POLYETHYLENE terephthalate, EPOXY resins, ANALYTICAL chemistry, FOURIER transform infrared spectroscopy
Abstract

In this study, a microbial–enzymatic strategy was pursued to address the challenge of degrading thermoplastic and thermosetting polymers. Environmental microorganisms were isolated, and their enzymatic activities were assessed using colorimetric assays to evaluate their potential for producing enzymes capable of degrading these polymers. Microorganisms demonstrating higher activity in the enzymatic assays were selected for a 30-day biodegradation experiment, in which epoxy resins, polyethylene terephthalate, or polystyrene served as the sole carbon source. The effectiveness of biodegradation was assessed through the ATR-FTIR analysis of the chemical composition and the SEM examination of surface characteristics before and after degradation. The results indicated that thermoplastic compounds were more susceptible to microbial degradation, exhibiting greater changes in absorbance. In particular, PET treated with Stenotrophomonas sp. showed the most significant efficacy, achieving a 60.18% reduction in the area under the curve with a standard error of ± 3.42 when analyzed by FTIR spectroscopy. Significant alterations in surface morphology were noticed in thermoplastic compounds. In contrast, thermosetting compounds demonstrated lower reactivity, as evidenced by the absence of band shifts in FTIR spectra and minor changes in bond absorbance and surface morphology. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

20. Aspergillus niger environmental isolates and their specific diversity through metabolite profiling

Author

Šimonovičová, Alexandra, Vojtková, Hana, Nosalj, Sanja, Piecková, Elena, Švehláková, Hana, Kraková, Lucia, Drahovská, Hana, Stalmachová, Barbara, Kučová, Kateřina, and Pangallo, Domenico

Subjects
extrolite profile, molecular analyses, Biolog FF MicroplateTM, food and beverages, multi-criteria data analysis, Aspergillus niger environmental isolates, Microbiology, QR1-502, Original Research
Abstract

We present a biological profile of 16 Aspergillus niger environmental isolates from different types of soils and solid substrates across a pH range, from an ultra-acidic (9.0) environment. The soils and solid substrates also differ in varying degrees of anthropic pollution, which in most cases is caused by several centuries of mining activity at old mining sites, sludge beds, ore deposits, stream sediments, and coal dust. The values of toxic elements (As, Sb, Zn, Cu, Pb) very often exceed the limit values. The isolates possess different macro- and micromorphological features. All the identifications of Aspergillus niger isolates were confirmed by molecular PCR analysis and their similarity was expressed by RAMP analysis. The biochemical profile of isolates based on FF-MicroPlate tests from the Biolog system showed identical biochemical reactions in 50 tests, while in 46 tests the utilisation reactions differed. The highest similarity of strains isolated from substrates with the same pH, as well as the most suitable biochemical tests for analysis of the phenotypic similarity of isolated strains, were confirmed when evaluating the biochemical profile using multicriterial analysis in the Canoco program. The isolates were screened for mycotoxin production by thin-layer chromatography (TLC), as well. Two of them were able to synthesise ochratoxin A, while none produced fumonisins under experimental conditions. Presence of toxic compounds in contaminated sites may affect environmental microscopic fungi and cause the genome alteration, which may result in changes of their physiology, including the production of different (secondary) metabolites, such as mycotoxins. Web of Science 12 art. no. 658010

Published
2021

33. Comparison of methods for identification of microbial communities in book collections: Culture-dependent (sequencing and MALDI-TOF MS) and culture-independent (Illumina MiSeq)

Author

Kraková, Lucia, primary, Šoltys, Katarína, additional, Otlewska, Anna, additional, Pietrzak, Katarzyna, additional, Purkrtová, Sabina, additional, Savická, Dana, additional, Puškárová, Andrea, additional, Bučková, Mária, additional, Szemes, Tomáš, additional, Budiš, Jaroslav, additional, Demnerová, Kateřina, additional, Gutarowska, Beata, additional, and Pangallo, Domenico, additional

Published
2018
Full Text
View/download PDF

40. Biogas production: evaluation of the influence of K2FeO4 pretreatment of maple leaves (Acer platanoides) on microbial consortia composition.

Author

Planý, Matej, Czolderová, Marianna, Kraková, Lucia, Puškárová, Andrea, Bučková, Mária, Šoltys, Katarína, Budiš, Jaroslav, Szemes, Tomáš, Mackulak, Tomáš, Wu, Jer-Horng, and Pangallo, Domenico

Abstract

The potential of K2FeO4 as a pretreatment agent of a lignocellulosic material was examined on leaves of Acer platanodides as the sole substrate for biogas production by anaerobic digestion carried out through modelling laboratory-scaled semi-continuous reactors differing in loading rates and substrate (pretreated and untreated leaves). The quality of bioagas produced by K2FeO4-pretreated leaves was significantly better in terms of higher methane content and lower content of H2S. K2FeO4 had no crucial influence on growth inhibition of biogas-producing bacteria, which were analysed by comprehensive culture-independent methods utilising high-throughput sequencing of specific genes [bacterial and archaeal 16S rRNA, formyltetrahydrofolate synthetase gene (fhs), methyl-coenzyme M reductase α subunit gene (mcrA) and fungal internal transcribed spacers (ITS)]. The higher amount of CH4 in biogas utilising pretreated leaves as substrate could be caused by a shift to acetoclastic methanogenesis pathway, which was indicated by the higher amount of homoacetogenic bacteria and acetotrophic methanogens detected in those reactors. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

47. Assessment of Microbial Diversity in Saudi Springs by Culture-Dependent and Culture-Independent Methods.

Author

Selim, Samy, Hassan, Sherif, Hagagy, Nashwa, Kraková, Lucia, Grivalský, Tomaš, and Pangallo, Domenico

Subjects
MICROBIAL diversity, WATER springs, BIODIVERSITY, THERMOPHILIC bacteria, THERMOPHILIC fungi
Abstract

Culture-dependent and culture-independent methods were used to evaluate the microbial diversity in two hot springs of the Aljouf region in Saudi Arabia, including Qasr Kaff and Ain Hawas. Physicochemical characteristics of the springs were examined to establish their effect on the biodiversity of thermophilic bacteria and fungi. We employed culture-dependent techniques to study microbial diversity using four different complex media for bacteria and fungi. In addition, the direct count for algal populations from two springs was investigated. We surveyed the microbial diversity in water and sediment samples from both springs by denaturing gradient gel electrophoresis (DGGE) and clone library construction. Bacillariophycaea (18 species) was the most diverse group, followed by Cyanophyceaea. Bacterial isolates closer to the generaBacillus spp., Geobacillus, Thermoactinomyces, and unidentified actinobacteria were recovered. Fungal isolates were related toAspergillus, Pezizaceae, Penicillium, Acremonium, Fusarium, Chrysosporium, andStachybotrys. Using molecular-based techniques, the results were slightly different from those obtained by culture-dependent methods, and more genera were obtained. However, most genera were uncultured microbes, particularly from bacterial communities. [ABSTRACT FROM PUBLISHER]

Published
2017
Full Text
View/download PDF

50. Prevalence of antibiotic-resistant coliform bacteria, Enterococcusspp. and Staphylococcusspp. in wastewater sewerage biofilm

Author

Lépesová, Kristína, Kraková, Lucia, Pangallo, Domenico, Medveďová, Alžbeta, Olejníková, Petra, Mackuľak, Tomáš, Tichý, Jozef, Grabic, Roman, and Birošová, Lucia

Abstract

•Wastewater treatment plant biofilms contain a higher number of antibiotic-resistant bacteria than wastewater.•Effluent biofilm contained the highest abundance of multidrug-resistant strains.•Bacteria from effluent biofilm have high biofilm-forming ability.•DGGE detected mainly Pseudomonas, Acinetobacterand Janthinobacteriumspp.•Predominant antibiotics in wastewater were azithromycin and clarithromycin.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results