Search

Your search keyword '"Krajcar V"' showing total 4 results
Start Over Author "Krajcar V"
4 results on '"Krajcar V"'

3. Adverse reactions in patients with B-cell lymphomas during combined treatment with rituximab: In vitro evaluation of rituximab hypersensitivity by basophil activation test.

Author

Piva E, Chieco-Bianchi F, Krajcar V, Aversa S, and Plebani M

Subjects
Adult, Aged, Aged, 80 and over, Antibodies, Monoclonal, Murine-Derived therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Dose-Response Relationship, Drug, Drug Hypersensitivity diagnosis, Drug Hypersensitivity immunology, Female, Humans, Male, Middle Aged, Rituximab, Tetraspanin 30 immunology, Antibodies, Monoclonal, Murine-Derived adverse effects, Antineoplastic Combined Chemotherapy Protocols adverse effects, Basophils immunology, Drug Hypersensitivity etiology, Flow Cytometry methods, Lymphoma, B-Cell drug therapy
Published
2012
Full Text
View/download PDF

4. Specific detection of cyclobutane pyrimidine dimers in phytoplankton DNA by a non-radioactive assay based on T4-endonuclease V digestion.

Author

Fafandel M, Bihari N, Krajcar V, Müller WE, Zahn RK, and Batel R

Subjects
Biological Assay, DNA Repair, Deoxyribonuclease (Pyrimidine Dimer), Phytoplankton chemistry, Sunlight, Ultraviolet Rays adverse effects, DNA Damage, Endodeoxyribonucleases metabolism, Petroleum adverse effects, Phytoplankton genetics, Pyrimidine Dimers analysis, Viral Proteins
Abstract

The effect of artificial and natural UV irradiation on DNA in marine phytoplankton Isochrysis galbana monoculture was investigated. The presence of cyclobutane pyrimidine dimers (CPDs) in unlabelled I. galbana DNA was detected by a non-radiometric alkaline filter elution assay after T4-endonuclease V digestion. The quantity of CPDs was estimated by alkaline agarose gel electrophoresis. Precise determination of the amount of DNA in the presence of I. galbana pigments was achieved by oxazole yellow homodimer (YOYO) dye. T4-endonuclease V-sensitive sites frequency (ESS/kb), measured after exposure to 2-40 kJ m(-2) of artificial UV light, increased in a dose-dependent manner. Twelve hours after irradiation cell culture growth was disrupted, and 50% of initial DNA damage in the cells was observed. After 1 h of sunlight exposure, the incidence of CPDs increase significantly. Prolonged exposition to sunlight decrease CPDs incidence due to efficiency of I. galbana DNA repair mechanisms. The presence of water-soluble crude oil fraction (WSOF) affected DNA repair efficiency resulting in accumulation of CPDs in I. galbana DNA.

Published
2001
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results