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459 results on '"Kouroumalis, E."'

1. Biomarkers for primary biliary cholangitis: current perspectives

Author

Kouroumalis E, Samonakis D, and Voumvouraki A

Subjects
Primary Biliary Cholangitis, Autoantibodies, microRNAs, proteomics, treatment response, Diseases of the digestive system. Gastroenterology, RC799-869
Abstract

Elias Kouroumalis,1 Demetrius Samonakis,2 Argyro Voumvouraki3 1Department of Gastroenterology, University Hospital and Medical School, University of Crete, Heraklion, Crete, Greece; 2Department of Gastroenterology, University Hospital of Heraklion, Crete, Greece; 3Department of Medicine, AHEPA University Hospital, Thessaloniki, Greece Abstract: Primary biliary cholangitis (PBC) is a chronic progressive cholestatic disease characterized by destruction of small- and medium-sized intrahepatic bile ducts. It is no longer a rare disease, since many new asymptomatic cases are incidentally identified. Liver biopsy is diagnostically critical but not always feasible or practical to be performed. Many potential, noninvasive, markers have been proposed to replace liver biopsy and further provide the assessment of disease severity and ultimate prognosis. In this review, we evaluated serum biomarkers proposed for diagnosis, extent of fibrosis, disease prognosis and attempts for early prediction of treatment response. Older biochemical and immunological markers are presented along with recent reports including the role of microRNAs and promising results based on proteomics and metabolomics. Keywords: primary biliary cholangitis, autoantibodies, microRNAs, proteomics, treatment response

Published
2018

2. The optical classification of the difficult sessile serrated lesions using convolutional neural networks trained with white light endoscopic images

Author

Panteris, V., additional, Feretzakis, G., additional, Karantanos, P., additional, Zoubouli, X., additional, Vgenopoulou, S., additional, Pierrakou, A., additional, Kalles, D., additional, Verykios, V., additional, Papalois, E. A., additional, Thomaidis, T., additional, Dalainas, I., additional, and Kouroumalis, E., additional

Published
2023
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6. Molecular Analysis of Hepatitis B Virus in Mothers-Children Pairs

Author

Galanakis E, Kouroumalis E, Iliopoulos I, Perdikogianni C, Sourvinos G, Paraskevis D, and Chatzidaki

Subjects
Hepatitis B virus, medicine, Biology, medicine.disease_cause, Virology, Molecular analysis
Abstract

ackground: Vertical transmission of Hepatitis B Virus (HBV) is the primary infection source for infants, but little is known on the proportion of children that have acquired HBV from their mothers. Objective: We investigated the relationship of HBV sequencing in HBVpositive children and their mothers and explored the HBV phylogenetic tree. Methods: Serum-extracted HBV-DNA from 38 individuals (13 children paired to nine mothers, 16 unpaired infected children) was amplified by polymerase chain reaction and the target region HBV surface glycoprotein (amino acids 40-171) was directly sequenced. Following editing and alignment of these sequences, phylogenetic tree analysis was performed using the neighbourjoining and maximum-likelihood methods. Results: Analysis was successfully performed in 29 subjects (23 children and six mothers), including six mother-child pairs. All individuals were infected by genotype D. Subgenotype adw3 prevailed (21, 72.4%), followed by ayw2 (4, 13.8%) and ayw3 (4, 13.8%). Among six mother-child pairs, three had identical and three had different subgenotypes. Phylogenetic analysis revealed that HBV sequences from three children did not cluster with their siblings suggesting a different source of infection. Conclusion: Our findings suggest that HBV subgenotypes in infected children may not be identical to their mothers’ and point to non-vertical HBV transmission in childhood.

Published
2021
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20. Understanding the interplay between COX-2 and hTERT in colorectal cancer using a multi-omics analysis

Author

Ayiomamitis, G.D. Notas, G. Vasilakaki, T. Tsavari, A. Vederaki, S. Theodosopoulos, T. Kouroumalis, E. Zaravinos, A.

Abstract

Background: Cyclooxygenase 2 (COX-2) is involved in the initial steps of colorectal cancer (CRC) formation, playing a key role in the catalysis of arachidonic acid to prostaglandin E2 (PGE2). The human telomerase reverse transcriptase (hTERT or TERT) also plays an important role in colorectal cancer growth, conferring sustained cell proliferation and survival. Although hTERT induces COX-2 expression in gastric and cervical cancer, their interaction has not been investigated in the context of CRC. Methods: COX-2, PGE2 levels, and telomerase activity were evaluated by immunohistochemistry, ELISA, and TRAP assay in 49 colorectal cancer samples. PTGS1, PTGS2, PTGES3, TERT mRNA, and protein levels were investigated using RNA-seq and antibody-based protein profiling data from the TCGA and HPA projects. A multi-omics comparison was performed between PTGS2 and TERT, using RNAseq, DNA methylation, copy number variations (CNVs), single nucleotide polymorphisms (SNPs), and insertions/deletions (Indels) data. Results: COX-2 expression was positive in 40/49 CRCs, bearing cytoplasmic and heterogeneous staining, from moderate to high intensity. COX-2 staining was mainly detected in the stroma of the tumor cells and the adjacent normal tissues. PGE2 expression was lower in CRC compared to the adjacent normal tissue, and inversely correlated to telomerase activity in right colon cancers. COX-1 and COX-2 were anticorrelated with TERT. Isoform structural analysis revealed the most prevalent transcripts driving the differential expression of PTGS1, PTGS2, PTGES3, and TERT in CRC. COX-2 expression was significantly higher among B-Raf proto-oncogene, serine/threonine kinase, mutant (BRAFmut) tumors. Kirsten ras oncogene (KRAS) mutations did not affect COX-2 or TERT expression. The promoter regions of COX-2 and TERT were reversely methylated. Conclusions: Our data support that COX-2 is involved in the early stages of colorectal cancer development, initially affecting the tumor’s stromal microenvironment, and, subsequently, the epithelial cells. They also highlight an inverse correlation between COX-2 expression and telomerase activity in CRC, as well as differentially methylated patterns within the promoter regions of COX-2 and TERT. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Published
2019

41. Influence of hepatitis G virus infection on liver disease

Author

Diamantis, I., Kouroumalis, E., Koulentaki, M., Fasler-Kan, E., Schmid, P., Hirsch, H., Bühler, H., Gyr, K., Battegay, M., Diamantis, I., Kouroumalis, E., Koulentaki, M., Fasler-Kan, E., Schmid, P., Hirsch, H., Bühler, H., Gyr, K., and Battegay, M.

Abstract

The influence of hepatitis G virus (HGV) infection on disease activity in hepatitis C related and unrelated liver disease was investigated in 254 individuals using an EIA polymerase chain reaction assay for HGV. One hundred patients had chronic hepatitis C, 26 primary biliary cirrhosis, and 30 alcoholic liver cirrhosis. In addition, 51 hepatitis B surface antigen (HBsAg)-positive and 47 anti-hepatitis C virus (HCV)-positive blood donors were screened. Hepatitis G virus was detected in 18% of patients with chronic hepatitis C, 13% of patients with alcoholic liver cirrhosis, 11 % of patients with primary biliary cirrhosis, 10% of anti-HCV-positive blood donors, and 2% of HBsAg-positive blood donors. Virus load and alanine aminotransferase (ALT) levels did not differ significantly in patients with HCV alone versus patients coinfected with HCV and HGV. However, mild liver fibrosis correlated with HGV coinfection. Hepatitis G virus did not influence ALT levels or liver damage in liver disease unrelated to viral infection

Published
2018

42. Validation and results of a questionnaire for functional bowel disease in out-patients

Author

Skordilis Panagiotis, Karachristos Andreas, Moschandreas Joanna, Fragkiadakis Nikolaos, Mouzas Ioannis A, Kouroumalis E, and Manousos Orestes N

Subjects
Public aspects of medicine, RA1-1270
Abstract

Abstract Background The aim was to evaluate and validate a bowel disease questionnaire in patients attending an out-patient gastroenterology clinic in Greece. Methods This was a prospective study. Diagnosis was based on detailed clinical and laboratory evaluation. The questionnaire was tested on a pilot group of patients. Interviewer-administration technique was used. One-hundred-and-forty consecutive patients attending the out-patient clinic for the first time and fifty healthy controls selected randomly participated in the study. Reliability (kappa statistics) and validity of the questionnaire were tested. We used logistic regression models and binary recursive partitioning for assessing distinguishing ability among irritable bowel syndrome (IBS), functional dyspepsia and organic disease patients. Results Mean time for questionnaire completion was 18 min. In test-retest procedure a good agreement was obtained (kappa statistics 0.82). There were 55 patients diagnosed as having IBS, 18 with functional dyspepsia (Rome I criteria), 38 with organic disease. Location of pain was a significant distinguishing factor, patients with functional dyspepsia having no lower abdominal pain (p < 0.001). Significant factors distinguishing between IBS and functional dyspepsia were relief of pain by either antacids or defecation (19% vs 71% and 66% vs 0% respectively). Awakening from pain at night was also a factor distinguishing between IBS and organic disease groups (26% vs 61%, p < 0.01). Conclusions This questionnaire for functional bowel disease is a valid and reliable instrument that can distinguish satisfactorily between organic and functional disease in an out-patient setting.

Published
2002
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46. TISSUE TRANSGLUTAMINASE ANTIBODIES AND COELIAC DISEASE IN PATIENTS WITH PRIMARY BILIARY CIRRHOSIS AND AUTOIMMUNE CHOLANGITIS

Author

Kouroumalis, E., Chatzikostas, K., Roussomoustakaki, M., Drygiannakis, D., Niniraki, M., Koulentaki, M., and Tzardi, M.

Subjects
Gastrointestinal diseases -- Research, Health, Research
Abstract

E. Kouroumalis [1] K. Chatzikostas [1] M. Roussomoustakaki [1] D. Drygiannakis [2] M. Niniraki [3] M. Koulentaki [1] M. Tzardi [4] (1.) Depts of Gastroenerology, (3.) Inmmunology, (4.) Pathology, University [...]

Published
2001

48. Increased prevalence of IgM anticardiolipin antibodies in patients with primary biliary cirrhosis

Author

Chatzicostas, C., Drygiannakis, D., Koutroubakis, I., Niniraki, M., Petinaki, E., Koulentaki, M., Dimoulios, P., and Kouroumalis, E.

Subjects
Biliary cirrhosis -- Care and treatment -- Research, Liver diseases -- Care and treatment -- Research, Immunoglobulin G -- Health aspects -- Research, Autoimmune diseases -- Care and treatment -- Research, Health, Care and treatment, Research, Health aspects
Abstract

Aim: In patients with non-autoimmune liver disease, anticardiolipin antibody (aCL) production is considered to be an epiphenomenon of liver damage not associated with thrombotic complications. There are sparse reports of [...]

Published
2004

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