1. Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism
- Author
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Masayuki Amagai, Shigeo Koyasu, Takayuki Ota, Miyo Aoki-Ota, Takeji Nishikawa, Kouji Simoda, and Kazuyuki Tsunoda
- Subjects
Immunology ,Naive B cell ,Molecular Sequence Data ,Immunoglobulin Variable Region ,Fluorescent Antibody Technique ,Autoimmunity ,Enzyme-Linked Immunosorbent Assay ,Mice, Transgenic ,Biology ,Autoantigens ,Immune tolerance ,Mice ,medicine ,Immune Tolerance ,Immunology and Allergy ,Animals ,Humans ,Amino Acid Sequence ,Antigen-presenting cell ,education ,B cell ,education.field_of_study ,B-Lymphocytes ,integumentary system ,Desmoglein 3 ,Peripheral tolerance ,Antibodies, Monoclonal ,General Medicine ,Flow Cytometry ,Adoptive Transfer ,Recombinant Proteins ,B-1 cell ,Cytoskeletal Proteins ,medicine.anatomical_structure ,Polyclonal B cell response ,Desmoplakins ,Immunoglobulin M ,Lymph Nodes ,Desmogleins ,Spleen - Abstract
To examine the mechanism of B cell tolerance against natural peripheral self-antigen, we generated transgenic mice expressing IgM specific for desmoglein 3 (Dsg3) from AK7 monoclonal antibody which itself does not induce blisters. Dsg3 is mainly expressed on stratified squamous epithelium and is the target antigen of an autoimmune bullous disease, pemphigus vulgaris. Transgenic B cells reactive to Dsg3 were observed in the spleen and lymph node. Although these B cells are autoreactive, they did not develop into B1 B cells. These B cells were functionally competent and anti-Dsg3 IgM was detected in the serum and on the keratinocyte cell surface. These results indicate that auto-reactive B cells against peripheral antigen (Dsg3) are able to develop in the presence of Dsg3 but are ignored by the immune system.
- Published
- 2004