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Your search keyword '"Kou, Rong-Wei"' showing total 14 results
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14 results on '"Kou, Rong-Wei"'

13. Phenolic and Steroidal Metabolites from the Cultivated Edible Inonotus hispidusMushroom and Their Bioactivities

Author

Kou, Rong-Wei, Du, Shuang-Tian, Xia, Bing, Zhang, Qiang, Yin, Xia, and Gao, Jin-Ming

Abstract

A chemical study on the fruiting bodies of cultivated edible mushroom Inonotus hispidusresulted in 14 metabolites including three new hispolon congeners, named inonophenols A–B and one new lanostane triterpenoid, named inonoterpene A. These structures were identified by NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and electronic circular dichroism (ECD) data analysis. All metabolites were assessed for neurotrophic, anti-inflammatory, and antioxidative activities. Among them, inonophenols B and C were the most active in promoting PC-12 cell neurite outgrowth at a concentration of 10 μM. The phenolic derivatives reduced NO generation by lipopolysaccharide (LPS)-induced BV-2 microglial cells by suppressing the expression of toll-like receptor-4 (TLR-4) and the nuclear factor-kappa-B (NF-κB) signaling pathway as well as the inflammatory mediators including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, the phenolics showed antioxidant effects in DPPH scavenging assay with the IC50values of 9.82–21.43 μM. These findings showed that I. hispidusmay be a new source of neurotrophic and protective agents against neurodegenerative disorders.

Published
2021
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14. New cyathane diterpenoids with neurotrophic and anti-neuroinflammatory activity from the bird's nest fungus Cyathus africanus.

Author

Yin, Xia, Wei, Jing, Wang, Wei-Wei, Gao, Yu-Qi, Stadler, Marc, Kou, Rong-Wei, and Gao, Jin-Ming

Subjects
*PROTEIN metabolism, *ANTI-inflammatory agents, *BIRDS, *CELL culture, *CELL receptors, *ANALYTICAL chemistry, *FUNGI, *MOLECULAR biology, *NERVE growth factor, *NONSTEROIDAL anti-inflammatory agents, *WESTERN immunoblotting, *NITRIC-oxide synthases, *CYCLOOXYGENASE 2, *CELL survival, *PHARMACODYNAMICS
Abstract

Eleven new cyathane diterpenoids, designated cyafricanins A–K (1 − 11), were isolated from the culture broth of the baisidiomycete Cyathus africanus (Nidulariaceae, Bird's nest fungi). Their structures were elucidated by comprehensive analysis of their NMR and HRESIMS data. Cyafricanins A (1) was found to possess an unusual 3,4- seco ‑carbon skeleton. All compounds were evaluated for their neurotrophic activity in PC-12 cells and anti-neuroinflammatory activity in BV2 microglia cells. All of the diterpenoids showed nerve growth factor induced neurite outgrowth-promoting activity at concentration of 20 μM. Among them, cyafricanin B (2) and cyafricanin G (7) exhibited promising neurotrophic activity, and cyafricanin A (1) showed strong inhibitory effects on both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Furthermore, molecular docking studies revealed that cyafricanin A (1) showed strong interactions with the iNOs protein in the active cavity. Unlabelled Image [ABSTRACT FROM AUTHOR]

Published
2019
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