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26 results on '"Kosztyu, Petr"'

4. Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation

Author

Turánek, Jaroslav, Kosztyu, Petr, Turánek Knötigová, Pavlína, Bartheldyová, Eliška, Hubatka, František, Odehnalová, Nikola, Mikulík, Robert, Vaškovicová, Naděžda, Čelechovská, Hana, Kratochvílová, Irena, Fekete, Ladislav, Tavares, Marina R., Chytil, Petr, Raška, Milan, and Etrych, Tomáš

Published
2024
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6. Myomedin replicas of gp120 V3 loop glycan epitopes recognized by PGT121 and PGT126 antibodies as non-cognate antigens for stimulation of HIV-1 broadly neutralizing antibodies

Author

Daniel Lišková, Veronika, primary, Kosztyu, Petr, additional, Kuchař, Milan, additional, Černý, Jiří, additional, Bharadwaj, Shiv, additional, Petroková, Hana, additional, Vroblová, Eliška, additional, Křupka, Michal, additional, Malý, Michal, additional, Zosinčuková, Tereza, additional, Šulc, Josef, additional, Rašková Kafková, Leona, additional, Raška, Milan, additional, and Malý, Petr, additional

Published
2022
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7. Network Analysis for Uncovering the Relationship between Host Response and Clinical Factors to Virus Pathogen: Lessons from SARS-CoV-2

Author

Sova, Milan, primary, Kudelka, Milos, additional, Raska, Milan, additional, Mizera, Jan, additional, Mikulkova, Zuzana, additional, Trajerova, Marketa, additional, Ochodkova, Eliska, additional, Genzor, Samuel, additional, Jakubec, Petr, additional, Borikova, Alena, additional, Stepanek, Ladislav, additional, Kosztyu, Petr, additional, and Kriegova, Eva, additional

Published
2022
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8. Galactose-Deficient IgA1 B cells in the Circulation of IgA Nephropathy Patients Carry Preferentially Lambda Light Chains and Mucosal Homing Receptors

Author

Zachova, Katerina, primary, Jemelkova, Jana, additional, Kosztyu, Petr, additional, Ohyama, Yukako, additional, Takahashi, Kazuo, additional, Zadrazil, Josef, additional, Orsag, Jiri, additional, Matousovic, Karel, additional, Galuszkova, Dana, additional, Petejova, Nadezda, additional, Mestecky, Jiri, additional, and Raska, Milan, additional

Published
2022
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11. Myomedin scaffold variants targeted to 10E8 HIV-1 broadly neutralizing antibody mimic gp41 epitope and elicit HIV-1 virus-neutralizing sera in mice

Author

Kuchař, Milan, primary, Kosztyu, Petr, additional, Daniel Lišková, Veronika, additional, Černý, Jiří, additional, Petroková, Hana, additional, Vróblová, Eliška, additional, Malý, Michal, additional, Vaňková, Lucie, additional, Křupka, Michal, additional, Rašková Kafková, Leona, additional, Turánek Knotigová, Pavlína, additional, Dušková, Jarmila, additional, Dohnálek, Jan, additional, Mašek, Josef, additional, Turánek, Jaroslav, additional, Raška, Milan, additional, and Malý, Petr, additional

Published
2021
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14. Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Author

Zachova, Katerina, primary, Kosztyu, Petr, additional, Zadrazil, Josef, additional, Matousovic, Karel, additional, Vondrak, Karel, additional, Hubacek, Petr, additional, Kostovcikova, Klara, additional, Tlaskalova Hogenova, Helena, additional, Mestecky, Jiri, additional, and Raska, Milan, additional

Published
2020
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16. Proteins mimicking epitope of HIV-1 virus neutralizing antibody induce virus-neutralizing sera in mice

Author

Kosztyu, Petr, primary, Kuchar, Milan, additional, Cerny, Jiri, additional, Barkocziova, Lucia, additional, Maly, Michal, additional, Petrokova, Hana, additional, Czernekova, Lydie, additional, Liskova, Veronika, additional, Raskova Kafkova, Leona, additional, Knotigova, Pavlina, additional, Masek, Josef, additional, Turanek, Jaroslav, additional, Maly, Petr, additional, and Raska, Milan, additional

Published
2019
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18. Možnosti a principy vakcinace proti covidu-19.

Author

Křupka, Michal, Kosztyu, Petr, Račanský, Mojmír, Schovánek, Jan, Sloupenská, Kristýna, Strojil, Jan, and Raška, Milan

Abstract

Copyright of Praktické Lékárenství is the property of SOLEN sro and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2021

20. Nonpyrogenic Molecular Adjuvants Based on norAbu-Muramyldipeptide and norAbu-Glucosaminyl Muramyldipeptide: Synthesis, Molecular Mechanisms of Action, and Biological Activities in Vitro and in Vivo

Author

Effenberg, Roman, primary, Turánek Knötigová, Pavlína, additional, Zyka, Daniel, additional, Čelechovská, Hana, additional, Mašek, Josef, additional, Bartheldyová, Eliška, additional, Hubatka, František, additional, Koudelka, Štěpán, additional, Lukáč, Róbert, additional, Kovalová, Anna, additional, Šaman, David, additional, Křupka, Michal, additional, Barkocziova, Lucia, additional, Kosztyu, Petr, additional, Šebela, Marek, additional, Drož, Ladislav, additional, Hučko, Michal, additional, Kanásová, Mária, additional, Miller, Andrew D., additional, Raška, Milan, additional, Ledvina, Miroslav, additional, and Turánek, Jaroslav, additional

Published
2017
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25. P-glycoprotein mediates resistance to A3 adenosine receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine-5′-n-methyluronamide in human leukemia cells.

Author

Mlejnek, Petr, Dolezel, Petr, and Kosztyu, Petr

Subjects
GLYCOPROTEINS, APOPTOSIS, CELL lines, CELL-mediated cytotoxicity, CELL death, CELL communication, TUMOR necrosis factors
Abstract

We studied effects of 2-chloro-N6-(3-iodobenzyl)-adenosine-5′-N-methyluronamide (Cl-IB-MECA) on apoptosis induction in the K562/Dox cell line, which overexpressed P-glycoprotein (P-gp, ABCB1, MDR1). We found that the K562/Dox cell line was significantly more resistant to Cl-IB-MECA than the maternal cell line K562, which did not express P-gp. Although both cell lines expressed the A3 adenosine receptor (A3AR), cytotoxic effects of Cl-IB-MECA were not prevented by its selective antagonist MRS1523 (3-propyl-6-ethyl-5-[(ethylthio)carbonyl]-2 phenyl-4-propyl-3-pyridine carboxylate). Analysis of cell extracts revealed that the intracellular level of Cl-IB-MECA was significantly lower in the K562/Dox cell line than in the maternal cell line K562. The downregulation of P-gp expression using shRNA targeting ABCB1 gene led to increased intracellular level of Cl-IB-MECA and restored cell sensitivity to this drug. Similarly, valspodar (PSC-833), a specific inhibitor of P-gp, restored sensitivity of the K562/Dox cell line to Cl-IB-MECA with concomitant increase of intracellular level of Cl-IB-MECA in the resistant cell line, while it affected cytotoxicity of Cl-IB-MECA in the sensitive cell line only marginally. An enzyme based assay provided evidence for interaction of P-gp with Cl-IB-MECA. We further observed that cytotoxic effects of Cl-IB-MECA could be augmented by activation of extrinsic cell death pathway by Apo-2L (TRAIL) but not FasL or TNF-α. Our results revealed that Cl-IB-MECA induced an increase in expression of TRAIL receptors in K562 cells, which could sensitize cells to apoptosis induction via an extrinsic cell death pathway. Importantly, these effects were inversely related to P-gp expression. In addition, MRS1523 did not affect Cl-IB-MECA induced expression of TRAIL receptors. J. Cell. Physiol. 227: 676-685, 2012. © 2011 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2012
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26. P-glycoprotein mediates resistance to A3 adenosine receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine-5'-n-methyluronamide in human leukemia cells.

Author

Mlejnek P, Dolezel P, and Kosztyu P

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics, Adenosine pharmacology, Adenosine A3 Receptor Agonists pharmacology, Adenosine Triphosphatases metabolism, Cell Death, Cell Line, Tumor, Cell Membrane drug effects, Cell Membrane metabolism, Dose-Response Relationship, Drug, Gene Expression Regulation, Neoplastic, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Adenosine analogs & derivatives, Drug Resistance, Neoplasm physiology, Leukemia drug therapy, Receptor, Adenosine A3 metabolism
Abstract

We studied effects of 2-chloro-N(6)-(3-iodobenzyl)-adenosine-5'-N-methyluronamide (Cl-IB-MECA) on apoptosis induction in the K562/Dox cell line, which overexpressed P-glycoprotein (P-gp, ABCB1, MDR1). We found that the K562/Dox cell line was significantly more resistant to Cl-IB-MECA than the maternal cell line K562, which did not express P-gp. Although both cell lines expressed the A3 adenosine receptor (A3AR), cytotoxic effects of Cl-IB-MECA were not prevented by its selective antagonist MRS1523 (3-propyl-6-ethyl-5-[(ethylthio)carbonyl]-2 phenyl-4-propyl-3-pyridine carboxylate). Analysis of cell extracts revealed that the intracellular level of Cl-IB-MECA was significantly lower in the K562/Dox cell line than in the maternal cell line K562. The downregulation of P-gp expression using shRNA targeting ABCB1 gene led to increased intracellular level of Cl-IB-MECA and restored cell sensitivity to this drug. Similarly, valspodar (PSC-833), a specific inhibitor of P-gp, restored sensitivity of the K562/Dox cell line to Cl-IB-MECA with concomitant increase of intracellular level of Cl-IB-MECA in the resistant cell line, while it affected cytotoxicity of Cl-IB-MECA in the sensitive cell line only marginally. An enzyme based assay provided evidence for interaction of P-gp with Cl-IB-MECA. We further observed that cytotoxic effects of Cl-IB-MECA could be augmented by activation of extrinsic cell death pathway by Apo-2L (TRAIL) but not FasL or TNF-α. Our results revealed that Cl-IB-MECA induced an increase in expression of TRAIL receptors in K562 cells, which could sensitize cells to apoptosis induction via an extrinsic cell death pathway. Importantly, these effects were inversely related to P-gp expression. In addition, MRS1523 did not affect Cl-IB-MECA induced expression of TRAIL receptors., (Copyright © 2011 Wiley Periodicals, Inc.)

Published
2012
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