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Fluctuations in the yield of wild berries are markedly influenced by weather conditions. However, the cause--effect relationship is often poorly understood. Based on data spanning a 20-year period in Finland, we made an effort to elucidate the influence of different weather conditions on the yield of arctic bramble (Rubus arcticus L.). We analyzed the regression coefficients of various weather conditions in several regression models using the elaboration approach. Temperature accumulated in July had a positive effect on yield. Yield was negatively influenced by temperature accumulated during the previous summer, rainfall in the October of the previous year, and temperature accumulated in May of the same year. It is notable that the same weather conditions had a positive influence on yield of the same year whereas these conditions had a negative effect on the yield potential of the following year. Compared with traditional analysis methods, the elaboration approach provided a better understanding of the relationship between weather parameters and yield. The rarity of a good yield could be explained by the particular vulnerability of arctic bramble to the negative effects of weather conditions. Some of these factors could be controlled in field conditions when cultivating arctic bramble. Keywords: Rubus arcticus, arctic bramble, yield fluctuation, weather, elaboration. Les fluctuations du rendement des recoltes de baies sauvages sont fortement influencees par les conditions meteorologiques. Toutefois, la relation cause a effet est souvent mal comprise. [Angstrom] partir de donnees s'echelonnant sur une periode de 20 ans en Finlande, les auteurs ont voulu elucider l'influence de differentes conditions meteorologiques sur le rendement du framboisier arctique (Rubus arcticus L.). Ils ont analyse les coefficients de regression de differentes conditions meteorologiques par differents modeles de regression selon l'approche par elaboration. La temperature accumulee en juillet exercait un effet positif sur le rendement. Le rendement etait negativement influence par la temperature accumulee au cours de l'ete precedent, les precipitations d'octobre de l'annee precedente et la temperature accumulee en mai de la meme annee. Il est remarquable que des conditions de temperatures identiques exercaient une influence positive sur la recolte de la meme annee alors que ces conditions exercaient un effet negatif sur le rendement potentiel de l'annee suivante. Comparative-ment aux methodes d'analyse traditionnelles, l'approche par elaboration a permis de mieux comprendre la relation entre les parametres meteorologiques et le rendement. La rarete d'un bon rendement pourrait s'expliquer par la vulnerabilite particuliere du framboisier arctique aux effets negatifs des conditions meteorologiques. Certains de ces facteurs pourraient etre contr[eth]les sur le terrain lors de la culture de framboisiers arctiques. [Traduit par la Redaction] Mots-cles : Rubus arcticus, framboisier arctique, fluctuation du rendement, temperature, elaboration., Introduction Arctic bramble (Rubus arcticus subsp. arcticus L.) is an herbaceous plant native to subarctic Eurasia but it produces berries primarily between 62[degrees] and 66[degrees] northern latitude (Hulten 1971; Ryynanen [...]

Using genetic markers, we investigated the genetic structure of three clonal aquatic moss species, Calliergon megalophyllum Mikut., Fontinalis antipyretica Hedw. and F. hypnoides Hartm. on two scales: among populations in a connected lake system (large-scale spatial genetic structure) and among individuals within populations (fine-scale spatial genetic structure). Mean genetic diversities per population were 0.138, 0.247 and 0.271, respectively, and total diversities equalled 0.223, 0.385 and 0.421, respectively. Relative differentiation levels (FST values of 0.173, 0.280 and 0.142, respectively) were significant but showed that there is a moderate amount of gene flow taking place within the lake system connected with narrow streams. Bayesian STRUCTURE analysis provided some indication that the direction of water flow influences population genetic structuring in the studied aquatic mosses. We propose that dispersal leading to gene flow in C. megalophyllum, F. antipyretica and F. hypnoides takes place both along water via connecting streams and by animal vectors, such as waterfowl. Nevertheless, the slight genetic structuring pattern along the direction of water flow suggests that dispersal of shoots or their fragments along water is a means of dispersal in these mosses. The absence of sexual reproduction and spores may have caused the observed spatial genetic structure within populations, including aggregations of similar genotypes (clones or closely related genotypes) at short distances in populations otherwise showing an isolation by distance effect. Regardless of the results pointing to the dominance of vegetative propagation, it is impossible to completely rule out the potential role of rare long-distance spore dispersal from areas where the species are fertile. [ABSTRACT FROM AUTHOR]

Chronic rhinosinusitis is one of the most common health care challenges, with significant direct medical costs and severe impact on lower airway disease and general health outcomes. The diagnosis of chronic rhinosinusitis (CRS) currently is based on clinical signs, nasal endoscopy and CT scanning, and therapeutic recommendations are focussing on 2 classes of drugs, corticosteroids and antibiotics. A better understanding of the pathogenesis and the factors amplifying mucosal inflammation therefore seems to be crucial for the development of new diagnostic and therapeutic tools. In an effort to extend knowledge in this area, the WP 2.7.2 of the GA2LEN network of excellence currently collects data and samples of 1000 CRS patients and 250 control subjects. The main objective of this project is to characterize patients with upper airway disease on the basis of clinical parameters, infectious agents, inflammatory mechanisms and remodeling processes. This collaborative research will result in better knowledge on patient phenotypes, pathomechanisms, and subtypes in chronic rhinosinusitis. This review summarizes the state of the art on chronic rhinosinusitis and nasal polyposis in different aspects of the disease. It defines potential gaps in the current research, and points to future research perspectives and targets. [ABSTRACT FROM AUTHOR]

Using respiratory protective equipment is one of the relevant preventive measures for infectious diseases, including COVID-19, and for various occupational respiratory hazards. Because experienced discomfort may result in a decrease in the utilization of respirators, it is important to enhance the material properties to resolve suboptimal usage. We combined several technologies to produce a filtration material that met requirements set by a cross-disciplinary interview study on the usability of protective equipment. Improved breathability, environmental sustainability, and comfort of the material were achieved by electrospinning poly(ethylene oxide) (PEO) nanofibers on a thin foam-formed fabric from regenerated cellulose fibers. The high filtration efficiency of sub-micron-sized diethylhexyl sebacate (DEHS) aerosol particles resulted from the small mean segment length of 0.35 μm of the nanofiber network. For a particle diameter of 0.6 μm, the filtration efficiency of a single PEO layer varied in the range of 80-97 % depending on the coat weight. The corresponding pressure drop had the level of 20-90 Pa for the airflow velocity of 5.3 cm/s. Using a multilayer structure, a very high filtration efficiency of 99.5 % was obtained with only a slightly higher pressure drop. This opens a route toward designing sustainable personal protective media with improved user experience., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Amidst the global momentum of behavioral insights (BI), there has been a shift from mostly nudge-based BI applications to systemic approaches. This is particularly pressing in public health, where interacting issues regularly produce unanticipated consequences. Regardless, little is known about adopting complex systems approaches in behavioral public policy. This study aims to capture current practices of international BI experts on the definition, application, drivers, and hindering factors in adopting complex systems approaches in public policy. Semi-structured individual expert interviews (n = 12) of international BI experts with extensive experience in educating, cooperating with, and/or advising public servants were analyzed with inductive content analysis. While the working definition of BI aligned with published definitions, experts varied in their descriptions of complex systems approaches and its significance for public policy, including socioecological aspects, systematic BI use across policy stages, recognizing intertwined behavior, and lack of ready-made solutions. They emphasized the importance of systems approaches, identifying drivers (e.g. need for a broader focus) and hindrances (e.g. pressure for quick results). Embracing complex systems in behavioral public policy provides a holistic perspective, extending beyond simple nudges, sometimes presumed as universally applicable. While complexity perspectives would align with policymakers' worldview, applications require more work to tailor to local situations and to evaluate. Recognizing that, given their distinct expertise content, BI expertise can be quite different from complex systems expertise. The field would benefit from clear descriptions and specialized training for effective integration and advocacy for these approaches., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society of Behavioral Medicine.)

Objectives: Planning is an effective self-regulation strategy. However, little is known why some people take up planning and some do not. Such understanding would help interventions to promote planning. We investigated how adolescents explain their (non) use of planning for physical activity after an intervention., Methods: Qualitative content analysis was employed to investigate follow-up interviews (a purposeful sampling; n = 19 low-to-moderately active, vocational school students) of Let's Move It trial participants twice post-intervention: 6-8 weeks and 14 months post-baseline. In the intervention, planning was one of the key techniques used to promote PA., Results: We identified seven categories linked to reasons for (not) using planning. Most were related to feelings anticipated to result from planning. Action- and identity-related concerns were also raised. The reasons for planning were that the plan (1) helps to clarify what to do and to get things done, (2) strengthens the feeling of autonomy, (3) promotes a sense of progress, ability and control over one's PA. The reasons for not planning were that (having) a plan may (1) feel forced and like an unpleasant duty, (2) take away life's spontaneity and freedom, (3) result in anticipated annoyance and bad mood if one fails to enact the plan, or (4) be an effective strategy for others but not for the interviewee., Conclusions: Planning may not only link to behavioural control but also the sense of autonomy, and thus subsequent motivation. We suggest various strategies to promote planning, including challenging non-planner identity and harnessing social dimension of planning., (© 2022 The Authors. British Journal of Health Psychology published by John Wiley & Sons Ltd on behalf of British Psychological Society.)

Mineral deposits containing commercially exploitable metals are of interest for seabed mineral extraction in both the deep sea and shallow sea areas. However, the development of seafloor mining is underpinned by high uncertainties on the implementation of the activities and their consequences for the environment. To avoid unbridled expansion of maritime activities, the environmental risks of new types of activities should be carefully evaluated prior to permitting them, yet observational data on the impacts is mostly missing. Here, we examine the environmental risks of seabed mining using a causal, probabilistic network approach. Drawing on a series of expert interviews, we outline the cause-effect pathways related to seabed mining activities to inform quantitative risk assessments. The approach consists of (1) iterative model building with experts to identify the causal connections between seabed mining activities and the affected ecosystem components and (2) quantitative probabilistic modeling. We demonstrate the approach in the Baltic Sea, where seabed mining been has tested and the ecosystem is well studied. The model is used to provide estimates of mortality of benthic fauna under alternative mining scenarios, offering a quantitative means to highlight the uncertainties around the impacts of mining. We further outline requirements for operationalizing quantitative risk assessments in data-poor cases, highlighting the importance of a predictive approach to risk identification. The model can be used to support permitting processes by providing a more comprehensive description of the potential environmental impacts of seabed resource use, allowing iterative updating of the model as new information becomes available.

Objectives: Intervention participants' responses to and engagement with interventions are a key intermediate step between interventions and intended outcomes. The aim of this study was to qualitatively investigate crucial aspects of engagement, namely acceptability (experienced cognitive and emotional responses to the intervention), receipt (comprehension of intervention content), and skill enactment (skill performance in target settings), within the Let's Move It, a multi-component school-based physical activity intervention., Design: A longitudinal qualitative study embedded in a cluster-randomized trial, with individual interviews of purposefully sampled intervention participants immediately post-intervention (n = 21) and at 14 months (n = 14)., Methods: Semi-structured interviews were analysed using thematic analysis. Abductive coding process was taken to identify categories for themes., Results: The analysis resulted in 12 themes and 18 subthemes. Overall, participants reported perceived effectiveness of and affective attitude towards the intervention (acceptability) and understood the main messages and skills (receipt). For example, findings indicated comprehension of the non-judgemental nature and choice-providing messages of the intervention underpinned by self-determination theory. Despite reporting understanding how and why to perform the skills, not using them was a highlighted theme (skill enactment), particularly for self-regulatory techniques such as planning. Friends' role as key self-motivation technique was a prevalent theme. In the within-individual analysis, three different engager types were identified: positive, ambivalent, and negative., Conclusion: Identifying misunderstandings and difficulties in skill acquisition can help interpret main trial outcomes and inform further intervention optimization. This study provides an example of how to use thematic analysis to assess acceptability, receipt, and enactment in interventions., (© 2020 The Authors. British Journal of Health Psychology published by John Wiley & Sons Ltd on behalf of British Psychological Society.)

Rationale: Trials evaluating interventions to promote health behavior change rarely embed investigations that assess participant perceptions of crucial triggers of change., Objective: The "Let's Move It" (LMI) randomized trial evaluated a theorybased whole school system intervention aiming to increase physical activity (PA) of adolescents attending vocational schools. This article serves two main purposes: to describe how to use the critical incident technique (CIT) to conduct in qualitative process evaluation to identify events, including intervention elements, which LMI trial participants perceived to enable or support behavior change., Method: Semi-structured interviews (n = 34) conducted immediately post intervention from intervention and control arms were analyzed using the CIT., Results: The analysis identified altogether 39 critical incidents. Most of the critical incidents were related to the LMI in the intervention arm and the findings are partly aligned with the LMI intervention theory. Analysis revealed several critical incidents also in the control arm, including gaining insights regarding PA and mere measurement effects, illustrating challenges facing real-world trials., Conclusion: The CIT seems a promising approach for directing analysis towards potentially crucial intervention elements as described by the participants themselves, helping in focusing and limiting the text corpus to accounts relevant to change. Qualitative evaluations in trials may add valuable understanding to complement quantitative assessments., (Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Background: Chronic rhinosinusitis with nasal polyposis (CRSwNP) and asthma coexist frequently and share similar features of inflammation and remodeling. Remodeling has become an important concept in the pathophysiology of asthma and CRSwNP. It happens early in the development of these diseases and is relatively resistant to treatments. The key enzymes responsible for remodeling are matrix metalloproteinases (MMPs). In this study we examined whether asthma and CRSwNP share similar MMP profiles., Methods: Nasal secretion and serum specimens of controls (19 subjects) and patients with asthma (12), CRSwNP (39), or both (16) were collected between December 2007 and May 2009. Groups were divided into 2 subgroups according to atopy. MMP-7, MMP-9, MMP-13, tissue inhibitors of metalloproteinases (TIMPs), TIMP-1 and TIMP-2, myeloperoxidase (MPO), and human neutrophil elastase (HNE) were measured using enzyme-linked immunosorbent assay (ELISA), and MMP-8 was determined using immunofluorometric assay. High-sensitivity C-reactive protein (hs-CRP) was measured to estimate systemic involvement., Results: Patients with asthma, CRSwNP, or both exhibited lower MMP-9, MMP-9/TIMP-1, MMP-9/TIMP-2, and MPO in nasal secretions (p < 0.05 in CRSwNP) and higher MMP-9, MMP-9/TIMP-1, MMP-9/TIMP-2, and HNE in serum (p < 0.05 in all groups) compared to controls, whereas no difference in MMP-7, MMP-13, TIMP-1, and TIMP-2 were detected. Atopy increased nasal MMP-9 and MPO expression. hs-CRP was higher in patients with CRSwNP and asthma compared to controls., Conclusion: Our findings suggest shared pathomechanisms behind asthma and CRSwNP. Contrasting local vs systemic results reflect a different ability of healthy mucosa to react to exogenous stimuli, possibly indicating a protective function of MMP-9 and possibly also MMP-8 in the airways., (© 2015 ARS-AAOA, LLC.)

This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mülleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus., (© 2010 Blackwell Publishing Ltd.)

Although the cost of high throughput sequencing is decreasing, the cost is still often too high for individual projects targeted at, e.g., genome-wide transcription profiling in non-model organisms. Then, a low-cost alternative is cDNA-AFLP, which we have now considerably modified in order to develop a faster and simpler method to identify and analyze genes involved in specific, possibly adaptive characteristics. Particularly, we wanted to exclude repetitive PCR amplifications, extensive cloning and the presence of overlapping transcripts, which all lower the efficiency of the method.

The main arctic bramble ( Rubus arcticus) cultivars are susceptible to downy mildew ( Peronospora sparsa), which seriously threatens the cultivation. The efficiency of Aliette, Euparen M, phosphite-containing Phosfik, Phostrol, Farm-Fos-44, and Kaliumfosfiet, as well as Bion was evaluated in the greenhouse. Fewer symptoms and less Peronospora DNA were found in plants treated with Euparen M and Bion, whereas Aliette, Phosfik, and Phostrol gave moderate protection. Three arctic bramble cultivars showed varying susceptibility to P. sparsa. An inexpensive and fast in vitro plate test gave results parallel with those obtained in the greenhouse. Quantitative differences were found in the phenolic profiles of the leaves of different cultivars and in different treatments. Several phenolic compounds were tentatively identified in arctic bramble for the first time, for example, monomeric and oligomeric ellagitannins and galloylglucoses. Negative correlation was found between the amount of P. sparsa DNA and flavonol glycosides and some ellagitannins in the leaves 8 days after inoculation, suggesting a possible role for these phenolics in the defense.

Two experiments were performed to test the relevance of bryophyte (Plantae, Bryophyta) material for forensic studies. The first experiment was conducted to reveal if, and how well, plant fragments attach to footwear in general. In the test, 16 persons walked outdoors wearing rubber boots or hiking boots. After 24h of use outdoors the boots were carefully cleaned, and all plant fragments were collected. Afterwards, all plant material was examined to identify the species. In the second experiment, fresh material of nine bryophyte species was kept in a shed in adverse conditions for 18 months, after which DNA was extracted and subjected to genotyping to test the quality of the material. Both experiments give support for the usability of bryophyte material in forensic studies. The bryophyte fragments become attached to shoes, where they remain even after the wearer walks on a dry road for several hours. Bryophyte DNA stays intact, allowing DNA profiling after lengthy periods following detachment from the original plant source. Based on these experiments, and considering the fact that many bryophytes are clonal plants, we propose that bryophytes are among the most usable plants to provide botanical evidence for forensic investigations.

A proportion of patients with chronic rhinosinusitis, especially if nasal polyps are present, have a diagnosis of fungal rhinosinusitis. The diagnosis is difficult to establish because the symptoms and clinical and radiological signs are non-specific. Also current diagnostic methods, i.e. histology, fungal staining and culture, are insensitive. The performance of the Aspergillus galactomannan (GM) ELISA and real-time PCR for Aspergillus fumigatus mitochondrial DNA was evaluated for the detection of Aspergillus in sinus mucus samples from 25 patients with chronic rhinosinusitis with nasal polyposis. The results were compared with those from nasal lavage fluid from 19 healthy volunteers. Seven patients (28 %) were diagnosed as having fungal rhinosinusitis according to the presence of filaments in histology or direct microscopy using Calcofluor white. All fungal rhinosinusitis patients were negative in the GM ELISA. GM ELISA was positive in five patients whose samples were negative using conventional methods and A. fumigatus PCR. Two out of seven patients with fungal rhinosinusitis were positive by A. fumigatus PCR: one also had a positive A. fumigatus culture, and one had hyphae consistent with Aspergillus in histology. One additional patient had a weak positive PCR result, but other fungal tests were negative. In control subjects, the GM ELISA was positive in 21 %, whereas direct microscopy, culture and A. fumigatus PCR were negative in all samples. Direct microscopy and culture together with histology remain pivotal in defining fungal rhinosinusitis diagnosis. A. fumigatus PCR may have additional value in allowing the diagnosis to be made sooner, whereas the GM ELISA is not reliable in diagnosing Aspergillus infection of the paranasal sinuses.

We have identified a fast and easy method for finding microsatellite markers that utilizes genome screening with inter-simple sequence repeat (ISSR) primers to detect microsatellite regions and to obtain sequence information flanking one side of the microsatellites and a restriction-ligation technique with a specific adaptor to allow sequence walking to obtain sequence information flanking the other side of the microsatellites. Two main alternatives of the method (with or without cloning) are presented. We successfully utilized the method when identifying microsatellite markers for 21 bryophyte species, three algal species, and for the raccoon dog. The proportion of polymorphic markers equaled 95%. We observed that microsatellites are commonly found within the sequenced ISSR amplification products (54% in the present study), in which case specific primers can be identified for the microsatellite without a further restriction-ligation step. It is evident that the DNA regions amplified by ISSR markers commonly represent microsatellite hotspots. We propose that the identified method and the knowledge of the common presence of additional microsatellite repeats within ISSR amplification products are especially attractive to researchers who conduct small-scale microsatellite identification, such as researchers in population genetics and conservation biology.

Background: Patients with chronic hyperplastic sinusitis (CHS) form a heterogeneous group with similar symptoms and similar treatment despite of possible different mechanisms behind the disease. In the present study we focused on the microbiological findings in CHS and compared these results to the patient history in order to find out a possible explanation for the aetiology and chronicity of CHS., Methods: In 30 patients the sinus mucus was collected under endoscopic sinus surgery. Samples from 20 healthy volunteers were collected by nasal lavage. Eosinophil staining, bacterial culturing and fungal staining and culturing were done. Histological samples were obtained from all patients., Results: Bacterial cultures were positive in 93% of the patients compared to 70% in controls. Staphylococcus aureus and coagulase-negative Staphylococci were the two most common findings in both groups. A total of seven patients had positive fungal finding. The only fungal genus found was Aspergillus. In the control group no samples were positive for fungi., Conclusions: Microbiological findings do not seem to explain the chronic course of CHS, but fungi may play some part in the pathophysiology of the disease. These results may be more a reflection of a change in the environment in the paranasal sinuses and a change in normal flora than the actual cause of CHS.