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Three-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

A series of experiments are described that provide recommendations of modifications to leaching test specifications to minimize losses of mercury from eluates during leaching tests and management of analytical samples. EPA methods of the Leaching Environmental Assessment Framework (LEAF) were used as a reference point for what steps are most likely to see losses, including filtration, storage of unpreserved mercury contaminated material, and materials of construction for vessels. The potential sorption of mercury to borosilicate glass and PTFE lab materials was tested to determine what materials are suitable to replace HDPE and polypropylene. The experimental findings indicate that bottles constructed of PTFE and Type I (borosilicate) glass are recommended as extraction and containment vessels for mercury-containing liquids. Type I syringe filtration of aqueous mercury samples at pH 2 through 9 through 0.45 µm PTFE filters housed in polypropylene is acceptable; however, sorption losses were observed to occur at pH > 9. Storage of unpreserved liquids containing mercury over extended leaching test intervals did not lead to significant losses of mercury when coupled with minimized headspace. All mercury-containing eluates should include preservation with Optima HNO3 to a 1 % concentration and gold (III) standard to a concentration of 200 µg/L, followed by refrigeration at

Abstract Background An important limiting factor in the development of centrally acting pharmaceuticals is the blood-brain barrier (BBB). Transport of therapeutic peptides through this highly protective physiological barrier remains a challenge for peptide drug delivery into the central nervous system (CNS). Because the most common strategy to treat moderate to severe pain consists of the activation of opioid receptors in the brain, the development of active opioid peptide analogues as potential analgesics requires compounds with a high resistance to enzymatic degradation and an ability to cross the BBB. Results Herein we report that tetrapeptide analogues of the type H-Dmt1-Xxx2-Yyy3-Gly4-NH2 are transported into the brain after intravenous and subcutaneous administration and are able to activate the μ- and δ opioid receptors more efficiently and over longer periods of time than morphine. Using the hot water tail flick test as the animal model for antinociception, a comparison in potency is presented between a side chain conformationally constrained analogue containing the benzazepine ring (BVD03, Yyy3: Aba), and a "ring opened" analogue (BVD02, Yyy3: Phe). The results show that in addition to the increased lipophilicity through amide bond N-methylation, the conformational constraint introduced at the level of the Phe3 side chain causes a prolonged antinociception. Further replacement of NMe-D-Ala2 by D-Arg2 in the tetrapeptide sequence led to an improved potency as demonstrated by a higher and maintained antinociception for AN81 (Xxx2: D-Arg) vs. BVD03 (Xxx2: NMe-D-Ala). A daily injection of the studied opioid ligands over a time period of 5 days did however result in a substantial decrease in antinociception on the fifth day of the experiment. The compact opioid agonist - NK1 antagonist hybrid SBCHM01 could not circumvent opioid induced tolerance. Conclusions We demonstrated that the introduction of a conformational constraint has an important impact on opioid receptor activation and subsequent antinociception in vivo. Further amino acid substitution allowed to identify AN81 as an opioid ligand able to access the CNS and induce antinociception at very low doses (0.1 mg/kg) over a time period up to 7 hours. However, tolerance became apparent after repetitive i.v. administration of the investigated tetrapeptides. This side effect was also observed with the dual opioid agonist-NK1 receptor antagonist SBCHM01.

Limited literature assessed the relation between family engagement in education and young children's socioemotional and behavioral functioning. This study investigated these associations longitudinally among urban and low-income, predominantly Latinx preschoolers (n = 69) and their mothers. Initial home-school conferencing predicted lower levels of child socioemotional and behavioral difficulties about 8 months later ([beta] = -0.20, p < 0.05). Additionally, initial child socioemotional and behavioral difficulties predicted higher levels of later home-based involvement ([beta] = 0.23, p < 0.05). Results highlighted bidirectional associations between these constructs and suggested potential avenues to enhance family engagement in education and reduce child socioemotional and behavioral problems.

Abstract Background The clinical treatment of various types of pain relies upon the use of opioid analgesics. However most of them produce, in addition to the analgesic effect, several side effects such as the development of dependence and addiction as well as sedation, dysphoria, and constipation. One solution to these problems are chimeric compounds in which the opioid pharmacophore is hybridized with another type of compound to incease antinociceptive effects. Neurotensin-induced antinociception is not mediated through the opioid system. Therefore, hybridizing neurotensin with opioid elements may result in a potent synergistic antinociceptor. Results Using the known structure-activity relationships of neurotensin we have synthesized a new chimeric opioid-neurotensin compound PK20 which is characterized by a very strong antinociceptive potency. The observation that the opioid antagonist naltrexone did not completely reverse the antinociceptive effect, indicates the partial involvement of the nonopioid component in PK20 in the produced analgesia. Conclusions The opioid-neurotensin hybrid analogue PK20, in which opioid and neurotensin pharmacophores overlap partially, expresses high antinociceptive tail-flick effects after central as well as peripheral applications.

Fibromyalgia (FM) is considered a multifactorial disorder/syndrome with not fully understood etiology. Chronic generalized pain is the main symptom. A broad spectrum of factors is proposed to ex-plain the etiology. Its multifactorial nature is inherently associated with challenges in diagnosis and therapy. Various evidence of etiology has been evaluated with the aim of establishing a novel therapeutic approach. The main issue in the diagnosis and management is to focus on the evaluation of strict diagnostic criteria to minimize under- and overdiagnosis. Fibromyalgia is a challenge for perioperative management because of the increased risk of possible complications and poorer out-comes, including postoperative pain chronification. The authors have proposed an up-to-date evaluation of perioperative management considering the current guidelines. Multimodal analgesia combined with tailored perioperative care is the most appropriate assessment. Interdisciplinary research with special interest in pain management, including perioperative medicine, seems to be the main theme for the future.

Midline catheters (MCs) are used to deliver intravenous therapy lasting over 5 days to patients in hospitals. However, the constant development of home and outpatient care is challenging medical teams to provide effective and safe planned therapy to patients under such conditions. We describe the first time an MC was used in outpatient pain management in Poland. A 60-year-old man presented to the Pain Management Clinic with a history of RCC of the left kidney and lumbar back pain radiating to the left knee joint. The person whose case is described below had poor peripheral veins. He intravenously received lidocaine for 10 days via a midline catheter with a good response.

Regional anaesthesia, referred to as regional blocks, is one of the most frequently used methods of anaesthesia for surgery and for pain management. Local anaesthetic drug should be administered as close to the nerve as possible. If administered too far away, this may result in insufficient block. If it is administrated too close, severe nerve damage can occur. Neurostimulation techniques and ultrasound imaging have improved the effectiveness and safety of blockade, but the risk of nerve injury with permanent nerve disfunction has not been eliminated. Intraneural administration of a local anaesthetic damages the nerve mechanically by the needle and the high pressure generated by the drug inside the nerve. In many studies, injection pressure is described as significantly higher for unintended intraneural injections than for perineural ones. In recent years, the concept of combining techniques (neurostimulation + USG imaging + injection pressure monitoring) has emerged as a method increasing safety and efficiency in regional anaesthesia. This study focuses on the contribution of nerve identification methods to improve the safety of peripheral nerve blocks by reducing the risk of neural damage.

The interaction of Neuropilin-1 (NRP-1) with vascular endothelial growth factor (VEGF) has been shown to promote angiogenesis under physiological and pathological conditions. Angiogenesis around tumors is a major factor allowing for their growth and spread. Disrupting NRP-1/VEGF complex formation is thus a promising pathway for the development of new anticancer pharmaceuticals. A large body of work has been produced in the last two decades detailing the development of inhibitors of NRP-1/VEGF complex formation. Among those were peptide A7R and its smaller derivatives KXXR and K(Har)XXR. It has been previously reported that replacement of the XX backbone with triazole residues has a positive effect on the proteolytic stability of inhibitors. It has also been reported that a higher dihedral angle range restriction of the XX backbone has a positive effect on the activity of inhibitors. In this work, we have designed new triazole derivatives of K(Har)XXR inhibitors with substitution allowing for higher range restriction of the XX backbone. The obtained peptidomimetics have greater activity than their less restricted counterparts. One of the newly obtained structures has greater affinity than the reference peptide A7R.

H1N1 influenza virus is still regarded as a serious pandemic threat. The most effective method of protection against influenza virus and the way to reduce the risk of epidemic or pandemic spread is vaccination. Influenza vaccine manufactured in a traditional way, though well developed, has some drawbacks and limitations which have stimulated interest in developing alternative approaches. In this study, we demonstrate that the recombinant H1 vaccine based on the hydrophilic haemagglutinin (HA) domain and produced in the yeast system elicited high titres of serum haemagglutination-inhibiting antibodies in mice. Transmission electron microscopy showed that H1 antigen oligomerizes into functional higher molecular forms similar to rosette-like structures. Analysis of the N-linked glycans using mass spectrometry revealed that the H1 protein is glycosylated at the same sites as the native HA. The recombinant antigen was secreted into a culture medium reaching approximately 10 mg/l. These results suggest that H1 produced in Pichia pastoris can be considered as the vaccine candidate against H1N1 virus.

Despite substantial evidence for the fit of the 3- and 4-factor models of Psychopathy Checklist-based ratings of psychopathy in adult males and adolescents, evidence is less consistent in adolescent females. However, prior studies used samples much smaller than recommended for examining model fit. To address this issue, we conducted a confirmatory factor analysis of 646 adolescent females to test the fit of the 3- and 4-factor models. We also investigated the fit of these models in more homogeneous subsets of the full sample to examine whether fit was invariant across geographical region and setting. Analyses indicated adequate fit for both models in the full sample and was generally acceptable for both models in North American and European subsamples and for participants in less restrictive (probation/detention/clinic) settings. However, in the incarcerated subsample, the 4-factor model achieved acceptable fit on only two of four indices. Although model fit was not invariant across continent or setting, invariance could be achieved in most cases by simply allowing factor loadings on a single Psychopathy Checklist: Youth Version (Forth, Kosson, & Hare, 2003) item to vary across groups. In summary, in contrast to prior studies with small samples, current findings show that both the 3- and 4-factor models fit adequately in a large sample of adolescent females, and the factor loadings are largely similar for North American and European samples and for long-term incarcerated and shorter-term incarcerated/probation/clinic samples. (Contains 3 tables, 1 figure and 5 footnotes.)

Interpersonal characteristics are core features of the psychopathy construct which have a unique pattern of correlations with a variety of external correlates. To improve the assessment of interpersonal traits, the current study evaluated the internal structure of the Interpersonal Measure of Psychopathy (IM-P) through exploratory and confirmatory factor analyses (CFA) in a large sample of jail inmates. A 17-item, 3-factor (Dominance, Grandiosity, and Boundary Violations) structure evidenced good fit in European American inmates. A second CFA demonstrated good fit for this structure in a sample of African American inmates. Moreover, a multigroup CFA indicated structural invariance between European and African American inmates. External validity was tested and demonstrated through positive correlations between IM-P factor scores and Psychopathy Checklist-Revised total and facet scores (R. D. Hare, 2003) and antisocial personality disorder symptoms and diagnoses. Modest correlations between Grandiosity scores and scores on the Shipley Institute of Living Scale-Revised (R. A. Zachary, 1994) were also observed. Finally, a step-down hierarchical regression was conducted to test for racial bias of the IM-P factor scores in relation to external correlates. Little evidence was found for slope bias, but there was evidence of intercept bias for some analyses. Implications and advantages of assessing psychopathy through a comprehensive evaluation of interpersonal traits are discussed. (Contains 6 tables and 6 footnotes.)

Substantial evidence exists for 3- and 4-factor models of psychopathy underlying patterns of covariation among the items of the Psychopathy Checklist-Revised (PCL-R) in diverse adult samples. Although initial studies conducted with the Psychopathy Checklist: Youth Version (PCL:YV) indicated reasonable fit for these models in incarcerated male adolescents in the United States and the United Kingdom, only one published study has addressed the factor structure of PCL:YV psychopathy in female adolescents, and no prior studies have addressed it outside of these countries. We used confirmatory factor analysis to investigate the factor structure underlying PCL:YV scores in 314 incarcerated (143 male, 171 female) and 193 in-school (99 male, 94 female) adolescents, ages 14 to 19 years. The 2-factor model provided adequate fit only for incarcerated male adolescents and the 4-factor model was problematic in all samples, but the 3-factor solution provided an adequate model in incarcerated and community male adolescents. None of the models provided consistently acceptable fit among female adolescents. Current findings provide evidence for the robustness of the 3-factor model of psychopathy in incarcerated and community male adolescent samples but raise doubts about the applicability of this model to female adolescents. (Contains 3 tables, 1 figure and 1 footnote.)

The power of scales based on the Psychopathy Checklist (PCL; R. D. Hare, 1980) for prediction of violent behavior is well established. Although evidence suggests that this relationship is chiefly due to the impulsive and antisocial lifestyle component (Factor 2), the predictive power of psychopathy for violence may also reflect the multiplicative effects of this component with interpersonal and unemotional traits (Factor 1). The determination of the extent to which psychopathy subcomponents interact to predict violence has theoretical and practical implications for PCL-assessed psychopathy. However, the relationship between violence and the interactive effects of psychopathy subcomponents remains largely undetermined. The authors used prospective and cross-sectional designs to examine the independent and interactive effects of the factors of PCL-assessed psychopathy in 2 samples: (a) 199 county jail inmates and (b) 863 civil psychiatric patients. The Factor 1 x Factor 2 interaction predicted violence in both samples, such that the predictive power of Factor 2 was attenuated at lower levels of Factor 1. (Contains 2 tables and 3 footnotes.)

Two studies are reported on the underlying dimensions of the psychopathy construct in adolescents as measured by the Hare Psychopathy Checklist-Youth Version (PCL: YV; Forth, Kosson, & Hare, 2003). In Study 1, the PCL: YV item ratings for 505 male adolescents incarcerated in 5 different settings in North America were used to test the fit of 3 models that have been hypothesized to represent the structure of psychopathy in adults. A 4th model based on parceling PCL: YV items was also tested. In Study 2, these models were tested with a sample of 233 male adolescents incarcerated in 2 facilities in the United Kingdom. Model fit results indicated that the 18-item 4-factor model developed by Hare (2003) and a modified version of a 13-item 3-factor model developed by Cooke and Michie (2001) were associated with generally good fit. Because the 4-factor model is a less saturated model than the 3-factor model (better parameter to data point ratio), it survived a riskier test of disconfirmation. Implications for the nature of psychopathy in youth are discussed.

The accuracy of the prediction of criminal violence may be improved by combining psychopathy with other variables that have been found to predict violence. Research has suggested that assessing intelligence (i.e., IQ) as well as psychopathy improves the accuracy of violence prediction. In the present study, the authors tested this hypothesis by using a contemporary measure of psychopathy, the Psychopathy Checklist-Revised (R. D. Hare, 2003), in a sample of 326 European American and 348 African American male offenders. The postdictive power of psychopathy was evident for both ethnic groups and robust across most changes in the operationalization of violence and the analysis conducted, whereas the postdictive power of IQ was not. No Psychopathy x IQ interactions were identified. Implications of these results for violence prediction are discussed.

The studies were conducted from 2012 to 2015 at the National Institute of Horticultural Research in Skierniewice, Poland. The aim of the research was to determine the yield of Chinese cabbage, its storage ability and nutritional value, depending on weed management methods used during cultivation. In the field experiments the following methods were compared: mechanical treatments, mechanical treatments + growth stimulators, soil mulching with black polypropylene, black polyethylene and biodegradable foil and hand weeding. After harvest the Chinese cabbage was stored at 0-2°C for 125-126 days depending on the year and after storage marketable and rotten heads were sorted. The percent of yellowed, rotten leaves in the total mass of the stored heads was also determined, as well as the natural weight loss. The chemical composition of Chinese cabbage was analyzed after harvest and after storage. The analyses included: dry matter, total sugars, vitamin C and soluble phenol content. After harvest the highest yield of Chinese cabbage grown in black polyethylene mulch was obtained. After storage the highest yield of marketable heads from cabbage mechanically weeded with additional application of biostimulator AlfaMax during cultivation was obtained. Chemical analyses showed that after harvest the highest dry matter, total sugars and vitamin C content were found in Chinese cabbage mechanically weeded and soluble phenols were the highest in non-weeded Chinese cabbage. After storage the highest content of dry matter was recorded in non-weeded Chinese cabbage, while total sugars were the highest in cabbage mulched with black polyethylene and biodegradable foil. Vitamin C was the highest in mechanically weeded and soluble phenols were the highest in hand-weeded cabbage. [ABSTRACT FROM AUTHOR]

Two studies involving 98 prisoners and 92 undergraduates examined whether increased attention to interpersonal behaviors would improve assessments of the personality core underlying psychopathology. Twenty-one items were developed as the Interpersonal Measure of Psychopathology. Measurement of interpersonal behavior appears to improve prediction of criteria linked to the personality core of psychopathology. (SLD)

Examines correlations between psychopathic traits and forms of self-reported sexual aggression in college men (N=378), as well as the relationship between sexual aggression and components of psychopathy. Results indicate that measures of both dimensions of psychopathy, identified in previous research, accounted for variance in self-reports of sexual aggression. (RJM)

Seedlings of four vegetable species (maize, tomato, radish and onion) were treated for 7 days with methyl jasmonate (MeJA) vapors. MeJA accelerated senescence process of plant tissues and accumulation of anthocyanins. The only exception were hypocotyls of radish, which was found to decrease of the anthocyanin content under the influence of MeJA. It has been shown that MeJA has a different impact on the content of free biogenic amines. In case of leaves and epicotyls of maize and tomato hypocotyls, MeJA had no effect on levels of putrescine (Put). The leaves of tomatoes have shown to increase the putrescine level as a result of the impact of MeJA vapors. However, in the tissues of radish and onion very large decline in putrescine and spermidine content under the influence of the phytohormone were observed. The presence of small amounts of spermine was found only in tissues of radish, and onion, which does not affect the use of MeJA. In tissues of maize the presence of a significant content of 2-phenylethylamine (PEA) were found. Use for 7 days of MeJA vapors resulted in 3-fold increase in the content of the PEA in maize leaves. Small levels of the amine has also been found in tomato hypocotyls, where use of MeJA caused reduction its content. Obtained results show a little relationship between the activity of the studied enzymes (ornithine decarboxylase (ODC), lysine decarboxylase (LDC) and tyrosine decarboxylase (TYDC)) and the contents of the amines in seedlings of four vegetable species. No free cadaverine and tyramine were found, and therefore probably both polyamines might be present as conjugates. Putrescine also may be present in bound form. Moreover, since putrescine can be synthesized directly from ornithine or indirectly from arginine via agmatine, the activity of ODC alone did not give a full picture of the impact of MeJA on Put accumulation.