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17 results on '"Kornelis, Dieuwke"'

2. Specificity of the Point-of-Care Urine Strip Test for Schistosoma Circulating Cathodic Antigen (POC-CCA) Tested in Non-Endemic Pregnant Women and Young Children

Author

Casacuberta-Partal, Miriam, primary, Beenakker, Margreet, additional, de Dood, Claudia J., additional, Hoekstra, Pytsje T., additional, Kroon, Lisa, additional, Kornelis, Dieuwke, additional, Corstjens, Paul, additional, Hokke, Cornelis H., additional, van Dam, Govert J., additional, Roestenberg, Meta, additional, and van Lieshout, Lisette, additional

Published
2021
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5. Effects of schistosomiasis on susceptibility to HIV-1 infection and HIV-1 viral load at HIV-1 seroconversion: A nested case-control study

Author

Downs, Jennifer A., primary, Dupnik, Kathryn M., additional, van Dam, Govert J., additional, Urassa, Mark, additional, Lutonja, Peter, additional, Kornelis, Dieuwke, additional, de Dood, Claudia J., additional, Hoekstra, Pytsje, additional, Kanjala, Chifundo, additional, Isingo, Raphael, additional, Peck, Robert N., additional, Lee, Myung Hee, additional, Corstjens, Paul L. A. M., additional, Todd, Jim, additional, Changalucha, John M., additional, Johnson, Warren D., additional, and Fitzgerald, Daniel W., additional

Published
2017
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8. Effects of schistosomiasis on susceptibility to HIV-1 infection and HIV-1 viral load at HIV-1 seroconversion: A nested case-control study.

Author

Dupnik, Kathryn M., Lee, Myung Hee, Jr.Johnson, Warren D., Fitzgerald, Daniel W., Downs, Jennifer A., Peck, Robert N., van Dam, Govert J., Kornelis, Dieuwke, Hoekstra, Pytsje, Urassa, Mark, Lutonja, Peter, Kanjala, Chifundo, Isingo, Raphael, Changalucha, John M., de Dood, Claudia J., Corstjens, Paul L. A. M., and Todd, Jim

Subjects
SCHISTOSOMIASIS, HIV-positive persons, IMMUNOREGULATION, SURVEYS, PATIENTS, DISEASE risk factors
Abstract

Background: Schistosomiasis affects 218 million people worldwide, with most infections in Africa. Prevalence studies suggest that people with chronic schistosomiasis may have higher risk of HIV-1 acquisition and impaired ability to control HIV-1 replication once infected. We hypothesized that: (1) pre-existing schistosome infection may increase the odds of HIV-1 acquisition and that the effects may differ between men and women, and (2) individuals with active schistosome infection at the time of HIV-1 acquisition may have impaired immune control of HIV-1, resulting in higher HIV-1 viral loads at HIV-1 seroconversion. Methology/Principal findings: We conducted a nested case-control study within a large population-based survey of HIV-1 transmission in Tanzania. A population of adults from seven villages was tested for HIV in 2007, 2010, and 2013 and dried blood spots were archived for future studies with participants’ consent. Approximately 40% of this population has Schistosoma mansoni infection, and 2% has S. haematobium. We tested for schistosome antigens in the pre- and post-HIV-1-seroconversion blood spots of people who acquired HIV-1. We also tested blood spots of matched controls who did not acquire HIV-1 and calculated the odds that a person with schistosomiasis would become HIV-1-infected compared to these matched controls. Analysis was stratified by gender. We compared 73 HIV-1 seroconverters with 265 controls. Women with schistosome infections had a higher odds of HIV-1 acquisition than those without (adjusted OR = 2.8 [1.2–6.6], p = 0.019). Schistosome-infected men did not have an increased odds of HIV-1 acquisition (adjusted OR = 0.7 [0.3–1.8], p = 0.42). We additionally compared HIV-1 RNA levels in the post-seroconversion blood spots in HIV-1 seroconverters with schistosomiasis versus those without who became HIV-infected in 2010, before antiretroviral therapy was widely available in the region. The median whole blood HIV-1 RNA level in the 15 HIV-1 seroconverters with schistosome infection was significantly higher than in the 22 without schistosomiasis: 4.4 [3.9–4.6] log10 copies/mL versus 3.7 [3.2–4.3], p = 0.017. Conclusions/Significance: We confirm, in an area with endemic S. mansoni, that pre-existing schistosome infection increases odds of HIV-1 acquisition in women and raises HIV-1 viral load at the time of HIV-1 seroconversion. This is the first study to demonstrate the effect of schistosome infection on HIV-1 susceptibility and viral control, and to differentiate effects by gender. Validation studies will be needed at additional sites. [ABSTRACT FROM AUTHOR]

Published
2017
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9. Tools for diagnosis, monitoring and screening ofSchistosomainfections utilizing lateral-flow based assays and upconverting phosphor labels

Author

CORSTJENS, PAUL L. A. M., primary, DE DOOD, CLAUDIA J., additional, KORNELIS, DIEUWKE, additional, TJON KON FAT, ELISA M., additional, WILSON, R. ALAN, additional, KARIUKI, THOMAS M., additional, NYAKUNDI, RUTH K., additional, LOVERDE, PHILIP T., additional, ABRAMS, WILLIAM R., additional, TANKE, HANS J., additional, VAN LIESHOUT, LISETTE, additional, DEELDER, ANDRÉ M., additional, and VAN DAM, GOVERT J., additional

Published
2014
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12. Tools for diagnosis, monitoring and screening of Schistosoma infections utilizing lateral-flow based assays and upconverting phosphor labels.

Author

CORSTJENS, PAUL L. A. M., DE DOOD, CLAUDIA J., KORNELIS, DIEUWKE, TJON KON FAT, ELISA M., WILSON, R. ALAN, KARIUKI, THOMAS M., NYAKUNDI, RUTH K., LOVERDE, PHILIP T., ABRAMS, WILLIAM R., TANKE, HANS J., VAN LIESHOUT, LISETTE, DEELDER, ANDRÉ M., VAN DAM, GOVERT J., and Stothard, Russell

Subjects
SCHISTOSOMA, BIOLOGICAL assay, PHOSPHORS, ANTIGENS, BLOOD serum analysis
Abstract

The potential of various quantitative lateral flow (LF) based assays utilizing up-converting phosphor (UCP) reporters for the diagnosis of schistosomiasis is reviewed including recent developments. Active infections are demonstrated by screening for the presence of regurgitated worm antigens (genus specific polysaccharides), whereas anti-Schistosoma antibodies may indicate ongoing as well as past infections. The circulating anodic antigen (CAA) in serum or urine (and potentially also saliva) is identified as the marker that may allow detection of single-worm infections. Quantitation of antigen levels is a reliable method to study effects of drug administration, worm burden and anti-fecundity mechanisms. Moreover, the ratio of CAA and circulating cathodic antigen (CCA) is postulated to facilitate identification of either Schistosoma mansoni or Schistosoma haematobium infections. The UCP-LF assays allow simultaneous detection of multiple targets on a single strip, a valuable feature for antibody detection assays. Although antibody detection in endemic regions is not a useful tool to diagnose active infections, it gains potential when the ratio of different classes of antibody specific for the parasite/disease can be determined. The UCP-LF antibody assay format allows this type of multiplexing, including testing a linear array of up to 20 different targets. Multiple test spots would allow detection of specific antibodies, e.g. against different Schistosoma species or other pathogens as soil-transmitted helminths. Concluding, the different UCP-LF based assays for diagnosis of schistosomiasis provide a collection of tests with relatively low complexity and high sensitivity, covering the full range of diagnostics needed in control programmes for mapping, screening and monitoring. [ABSTRACT FROM PUBLISHER]

Published
2014
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14. Schistosoma mansoni: Analysis of monoclonal antibodies reactive with gut-associated antigens.

Author

Dam, Govert, Kornelis, Dieuwke, Zeyl, René, Rotmans, J., and Deelder, André

Abstract

The analysis of a series of monoclonal antibodies (mAbs) developed in our laboratory against gutassociated antigens of Schistosoma mansoni is described. It was found that mAbs that recognized epitopes of antigens in the gut and on the eggshell were mainly of the IgM isotype; these epitopes are likely to be carbohydrate in composition. Of a number of mAbs that were reactive with antigens important to the human humoral immune response, 75% appeared to be reactive with the circulating cathodic antigen. [ABSTRACT FROM AUTHOR]

Published
1993
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15. Up-Converting Phosphor Technology-Based Lateral Flow Assay for Detection of SchistosomaCirculating Anodic Antigen in Serum

Author

Corstjens, Paul L. A. M., van Lieshout, Lisette, Zuiderwijk, Michel, Kornelis, Dieuwke, Tanke, Hans J., Deelder, Andre M., and van Dam, Govert J.

Abstract

ABSTRACTSchistosomasp. circular anodic antigen (CAA) serum concentrations reflect actual worm burden in a patient and are a valuable tool for population screening and epidemiological research. However, for the diagnosis of individual imported schistosomiasis cases, the current enzyme-linked immunosorbent assay (ELISA) lacks sensitivity and robustness. Therefore, a lateral flow (LF) assay was developed to test CAA in serum for individual diagnosis of imported active schistosome infections. Application of fluorescent submicron-sized up-converting phosphor technology (UPT) reporter particles increased analytical sensitivity compared to that of the standard ELISA method. Evaluation of the UPT-LF test with a selection of 40 characterized epidemiologic samples indicated a good correlation between signal intensity and infection intensity. Subsequently, the UPT-LF assay was applied to 166 serum samples of Dutch residents (immigrants and travelers) suspected of schistosomiasis, a case in which group routine antibody detection frequently fails straightforward diagnosis. The UPT-LF assay identified 36 CAA-positive samples, compared to 15 detected by CAA-ELISA. In conclusion, the UPT-LF assay is a low-complexity test with higher sensitivity than the CAA-ELISA, well suited for laboratory diagnosis of individual active Schistosomainfections.

Published
2008
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