1. Study of the Dynamics of Biofilm Formation and Elastase Activity of Pseudomonas aeruginosa in the Presence of Dodecanoyl-Homoserine Lactone.
- Author
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Cherepushkina VS, Mironova TE, Afonyushkin VN, Koptev VY, Nefedova EV, Donchenko NA, and Dimova AS
- Subjects
- 4-Butyrolactone biosynthesis, 4-Butyrolactone pharmacology, Bacterial Load, Biofilms growth & development, Culture Media chemistry, Culture Media pharmacology, Dose-Response Relationship, Drug, Homoserine biosynthesis, Homoserine pharmacology, Pseudomonas aeruginosa enzymology, Pseudomonas aeruginosa growth & development, Quorum Sensing physiology, 4-Butyrolactone analogs & derivatives, Bacterial Proteins metabolism, Biofilms drug effects, Homoserine analogs & derivatives, Pancreatic Elastase metabolism, Pseudomonas aeruginosa drug effects
- Abstract
We studied the effect of early accumulation of N-3-oxo-dodecanoyl-homoserine lactone on the suppression of Pseudomonas aeruginosa reproduction, biofilm formation, and elastase activity. N-3-oxo-dodecanoyl-homoserine lactone in various concentrations was added to the P. aeruginosa culture, and changes in the concentration of bacteria and the formation of biofilms were studied in dynamics. N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 25 μM, decelerated proliferation of bacterial cells during the first 6 h of culturing (p<0.05) and stimulated biofilm formation after 18 h of culturing. Elastase activity of P. aeruginosa increased significantly after addition of N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 0.75 μM., (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2021
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