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17 results on '"Koonyosying, Pongpisid"'

2. Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach

Author

Sthitmatee, Nattawooti, Tankaew, Pallop, Modethed, Wittawat, Rittipornlertrak, Amarin, Muenthaisong, Anucha, Apinda, Nisachon, Koonyosying, Pongpisid, Nambooppha, Boondarika, Chomjit, Paweena, Sangkakam, Kanokwan, Singhla, Tawatchai, Vinitchaikul, Paramintra, Boonsri, Kittikorn, Pringproa, Kidsadagon, Punyapornwithaya, Veerasak, and Kreausukon, Khwanchai

Published
2023
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3. Efficiency of NHEJ-CRISPR/Cas9 and Cre-LoxP Engineered Recombinant Turkey Herpesvirus Expressing Pasteurella multocida OmpH Protein for Fowl Cholera Prevention in Ducks

Author

Apinda, Nisachon, primary, Yao, Yongxiu, additional, Zhang, Yaoyao, additional, Muenthaisong, Anucha, additional, Sangkakam, Kanokwan, additional, Nambooppha, Boondarika, additional, Rittipornlertrak, Amarin, additional, Koonyosying, Pongpisid, additional, Nair, Venugopal, additional, and Sthitmatee, Nattawooti, additional

Published
2023
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4. Simultaneous Protective Immune Responses of Ducks against Duck Plague and Fowl Cholera by Recombinant Duck Enteritis Virus Vector Expressing Pasteurella multocida OmpH Gene

Author

Apinda, Nisachon, primary, Muenthaisong, Anucha, additional, Chomjit, Paweena, additional, Sangkakam, Kanokwan, additional, Nambooppha, Boondarika, additional, Rittipornlertrak, Amarin, additional, Koonyosying, Pongpisid, additional, Yao, Yongxiu, additional, Nair, Venugopal, additional, and Sthitmatee, Nattawooti, additional

Published
2022
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7. Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response

Author

Rittipornlertrak, Amarin, primary, Nambooppha, Boondarika, additional, Muenthaisong, Anucha, additional, Apinda, Nisachon, additional, Koonyosying, Pongpisid, additional, Srisawat, Wanwisa, additional, Chomjit, Paweena, additional, Sangkakam, Kanokwan, additional, Punyapornwithaya, Veerasak, additional, Tiwananthagorn, Saruda, additional, Yokoyama, Naoaki, additional, and Sthitmatee, Nattawooti, additional

Published
2022
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14. Plasmodium parasites mount an arrest response to dihydroartemisinin, as revealed by whole transcriptome shotgun sequencing (RNA-seq) and microarray study

Author

Shaw, Philip J., primary, Chaotheing, Sastra, additional, Kaewprommal, Pavita, additional, Piriyapongsa, Jittima, additional, Wongsombat, Chayaphat, additional, Suwannakitti, Nattida, additional, Koonyosying, Pongpisid, additional, Uthaipibull, Chairat, additional, Yuthavong, Yongyuth, additional, and Kamchonwongpaisan, Sumalee, additional

Published
2015
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15. Measurement of Babesia bovis infected red blood cells using flow cytometry.

Author

Koonyosying, Pongpisid, Srichairatanakool, Somdet, Tiwananthagorn, Saruda, and Sthitmatee, Nattawooti

Subjects
*BABESIA, *ERYTHROCYTES, *DRUG use testing, *MICROSCOPY, *RAPID tooling
Abstract

Rapid and accurate tools are needed for high-throughput in vitro antibabesial drug testing. In this study, flow cytometry for the measuring of Babesia bovis in vitro culture, was developed using SYBR Green I and compared against the results of fluorescence-based assay and microscopic assay. A high correlation of measured parasitemia was observed with high R2 value (R2 = 0.9991) between flow cytometry and microscopic analysis. The degree of antibabesial drug sensitivity against B. bovis determined by flow cytometry was 0.424 ± 0.173 μM. Similar to the results of previously published studies involving fluorescence spectrometry-based assay (0.408 ± 0.011 μM) and microscopy-based assay (0.400 ± 0.017 μM). The outcomes of this present study suggest that flow cytometry assay using SYBR Green I can potentially be useful in determining parasitemia and can serve as a rapid alternative method to antibabesial drug testing. [Display omitted] • Flow cytometry assay using SYBR Green I was developed to measure B. bovis -infected red blood cells in an in vitro culture. • Flow cytometry assay using SYBR Green I was found to be potentially useful in determining the degree of parasitemia and approved of as a rapid alternative method to antibabesial drug testing. • Flow cytometry using SYBR Green I can be used to determine the IC 50 results to a degree that was similar to the results of previously published studies involving fluorescence-based assay and microscopy. [ABSTRACT FROM AUTHOR]

Published
2023
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16. Inhibition of Glutathione Biosynthesis Sensitizes Plasmodium bergheito Antifolates

Author

Songsungthong, Warangkhana, Koonyosying, Pongpisid, Uthaipibull, Chairat, and Kamchonwongpaisan, Sumalee

Abstract

ABSTRACTGlutathione plays a central role in maintaining cellular redox homeostasis, and modulations to this status may affect malaria parasite sensitivity to certain types of antimalarials. In this study, we demonstrate that inhibition of glutathione biosynthesis in the Plasmodium bergheiANKA strain through disruption of the γ-glutamylcysteine synthetase (γ-GCS) gene, which encodes the first and rate-limiting enzyme in the glutathione biosynthetic pathway, significantly sensitizes parasites in vivoto pyrimethamine and sulfadoxine, but not to chloroquine, artesunate, or primaquine, compared with control parasites containing the same pyrimethamine-resistant marker cassette. Treatment of mice infected with an antifolate-resistant P. bergheicontrol line with a γ-GCS inhibitor, buthionine sulfoximine, could partially abrogate pyrimethamine and sulfadoxine resistance. The role of glutathione in modulating the malaria parasite's response to antifolates suggests that development of specific inhibitors against Plasmodiumγ-GCS may offer a new approach to counter Plasmodiumantifolate resistance.

Published
2016
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17. The Plasmodium berghei RC strain is highly diverged and harbors putatively novel drug resistance variants.

Author

Songsungthong W, Kulawonganunchai S, Wilantho A, Tongsima S, Koonyosying P, Uthaipibull C, Kamchonwongpaisan S, and Shaw PJ

Abstract

Background: The current first line drugs for treating uncomplicated malaria are artemisinin (ART) combination therapies. However, Plasmodium falciparum parasites resistant to ART and partner drugs are spreading, which threatens malaria control efforts. Rodent malaria species are useful models for understanding antimalarial resistance, in particular genetic variants responsible for cross resistance to different compounds., Methods: The Plasmodium berghei RC strain ( Pb RC) is described as resistant to different antimalarials, including chloroquine (CQ) and ART. In an attempt to identify the genetic basis for the antimalarial resistance trait in Pb RC, its genome was sequenced and compared with five other previously sequenced P. berghei strains., Results: We found that Pb RC is eight-fold less sensitive to the ART derivative artesunate than the reference strain Pb ANKA. The genome of Pb RC is markedly different from other strains, and 6,974 single nucleotide variants private to Pb RC were identified. Among these Pb RC private variants, non-synonymous changes were identified in genes known to modulate antimalarial sensitivity in rodent malaria species, including notably the ubiquitin carboxyl-terminal hydrolase 1 gene. However, no variants were found in some genes with strong evidence of association with ART resistance in P. falciparum such as K13 propeller protein., Discussion: The variants identified in Pb RC provide insight into P. berghei genome diversity and genetic factors that could modulate CQ and ART resistance in Plasmodium spp., Competing Interests: The authors declare that they have no competing interests.

Published
2017
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