147 results on '"Konomi H"'
Search Results
2. Infrasternal mediastinoscopic surgery for anterior mediastinal masses
- Author
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Uchiyama, A., Shimizu, S., Murai, H., Ohshima, A., Konomi, H., Ogura, Y., Ishikawa, N., Yamashita, H., Matsumoto, S., Kuroki, S., and Tanaka, M.
- Published
- 2004
- Full Text
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3. Laparoscopic pancreatic surgery: Current indications and surgical results
- Author
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Shimizu, S., Tanaka, M., Konomi, H., Mizumoto, K., and Yamaguchi, K.
- Published
- 2004
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4. Urine diacetylspermine as a novel tumour maker for pancreatobiliary carcinomas
- Author
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Yamaguchi, K., Nakamura, M., Shirahane, K., Konomi, H., Torata, N., Hamasaki, N., Kawakita, M., and Tanaka, M.
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- 2005
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5. Induction of duodenal motility activates the sphincter of Oddi (SO)-duodenal reflex in the Australian possum in vitro
- Author
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Konomi, H., Simula, M. E., Meedeniya, A. C. B., Toouli, J., and Saccone, G. T. P.
- Published
- 2002
6. Inhibitory motor innervation of the gall bladder musculature by intrinsic neurones containing nitric oxide in the Australian Brush-tailed possum (Trichosurus vulpecula)
- Author
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Meedeniya, A C B, Al-Jiffry, B O, Konomi, H, Schloithe, A C, Toouli, J, and Saccone, G T P
- Published
- 2001
7. Consideration of optimal postoperative adjuvant therapy for HER2-positive, Stage I pT1N0 cases
- Author
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Tanaka, H., primary, Umeda, S., additional, Nishimura, S., additional, Sadanaga, K., additional, Murakami, S., additional, Konomi, H., additional, Uchiyama, A., additional, and Tanaka, T., additional
- Published
- 2019
- Full Text
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8. P001 - Consideration of optimal postoperative adjuvant therapy for HER2-positive, Stage I pT1N0 cases
- Author
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Tanaka, H., Umeda, S., Nishimura, S., Sadanaga, K., Murakami, S., Konomi, H., Uchiyama, A., and Tanaka, T.
- Published
- 2019
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9. Young Japanese Underweight Women with 'Cinderella Weight' Are Prone to Malnutrition, including Vitamin Deficiencies
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Katsumi Iizuka, Hiroko Sato, Kazuko Kobae, Kotone Yanagi, Yoshiko Yamada, Chihiro Ushiroda, Konomi Hirano, Satomi Ichimaru, Yusuke Seino, Akemi Ito, Atsushi Suzuki, Eiichi Saitoh, and Hiroyuki Naruse
- Subjects
undernutrition ,dietary diversity score ,25-OH vitamin D ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Undernutrition among young women at “Cinderella weight” is socially important in Japan. To determine the nutritional status of Cinderella-weight women, we conducted an exploratory cross-sectional study on the health examination results of employees aged 20 to 39 (n = 1457 and 643 for women and men, respectively). The percentage of underweight women was found to be much higher than that of men (16.8% vs. 4.5%, respectively). In underweight women (n = 245), handgrip strength (22.82 ± 5.55 vs. 25.73 ± 5.81 kg, p < 0.001), cholesterol level (177.8 ± 25.2 vs. 194.7 ± 31.2 mg/dL, p < 0.05), and lymphocyte count (1883 ± 503 vs. 2148 ± 765/μL, p < 0.001) were significantly lower than in overweight women (n = 116). Then, the BMI < 17.5 group (n = 44) was referred to the outpatient nutrition evaluation clinic. Lower prealbumin, cholesterol, and lymphocyte levels were also observed in 34%, 59%, and 32% of the patients, respectively. Regarding dietary characteristics, 32% of the underweight women in this study skipped breakfast, and 50% had low dietary diversity scores. Lower total energy intake, carbohydrate and fiber intake, and Ca and Fe intake were also observed in 90% of the patients. Deficiencies in vitamin B1, B12, D, and folate were diagnosed in 4.6%, 25%, 14%, and 98% of the patients, respectively. Thus, young underweight women may be prone to malnutrition.
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- 2023
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10. Sonic hedgehog is an early developmental marker of intraductal papillary mucinous neoplasms: clinical implications of mRNA levels in pancreatic juice
- Author
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Ohuchida, K, primary, Mizumoto, K, additional, Fujita, H, additional, Yamaguchi, H, additional, Konomi, H, additional, Nagai, E, additional, Yamaguchi, K, additional, Tsuneyoshi, M, additional, and Tanaka, M, additional
- Published
- 2006
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11. Analysis of Matrix Protein Components of the Dermis-Like Structure Formed in a Long-Term Culture of Human Fibroblasts: Type VI Collagen Is a Major Component
- Author
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Hazeki, N., primary, Yamato, M., additional, Imamura, Y., additional, Sasaki, T., additional, Nakazato, K., additional, Yamamoto, K., additional, Konomi, H., additional, and Hayashi, T., additional
- Published
- 1998
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12. Diaspirin crosslinked human hemoglobin stimulates duodenal and biliary motility with a reduction in trans-sphincteric flow in the Australian brushtailed possum
- Author
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Irvine, C.M., primary, Meedeniya, A.C.B., additional, Konomi, H., additional, Schloithe, A.C., additional, Toouli, J., additional, and Saccone, G.T.P., additional
- Published
- 1998
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13. Characterization of the collagen in the hexagonal lattice of Descemet's membrane: its relation to type VIII collagen.
- Author
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Sawada, H, primary, Konomi, H, additional, and Hirosawa, K, additional
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- 1990
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14. Laparoscopic pancreatic surgery.
- Author
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Shimizu, S., Tanaka, M., Konomi, H., Mizumoto, K., and Yamaguchi, K.
- Subjects
LAPAROSCOPIC surgery ,LAPAROSCOPY ,PANCREATIC diseases ,PANCREATECTOMY ,GASTROSTOMY ,CYSTS (Pathology) - Abstract
Background: Although minimally invasive surgery has achieved worldwide acceptance in various fields, laparoscopic surgery for pancreatic diseases has been reported only rarely. The purpose of this study was to evaluate the outcomes and feasibility of laparoscopic pancreatic surgery. Methods: Fifteen patients, comprising eight men and seven women with an average age of 54 years, underwent laparoscopic pancreatic surgery. Distal pancreatectomy was indicated for solid tumors (n = 4), cystic lesions (n = 3), and chronic pancreatitis (n = 2). Cystogastrostomy was performed for pseudocysts (n = 4) and enucleation for insulinomas (n = 2). The lesions varied in size from 1 to 9 cm (2.9 ± 2.4 cm) and were located in the pancreatic head (n = 2), body (n = 3), or tail (n = 10). For distal pancreatectomy, the splenic artery was divided and the parenchyma was transected with a linear stapler. Laparoscopic ultrasonography was used to determine the distance between the tumor and the main pancreatic duct for enucleation as well as to localize the lesion for distal pancreatectomy. Cystogastrostomy, 4.5 cm in length, was also performed with the linear stapler through the window of the lesser omentum. Results: Mean operation time was 249 ± 70 min (293 ± 58 min in distal pancreatectomy, 185 ± 14 min in enucleation, 204 ± 50 min in cystogastrostomy), and mean blood loss was 138 ± 184 g (213 ± 227 g, 75 ± 35 g, 38 ± 48 g, respectively). Two distal pancreatectomies (13%) were converted to open surgery due to severe peripancreatic inflammation. There was no related mortality, but there were two cases (15%) of pancreatic fistula, one in a distal pancreatectomy case and the other in an enucleation case, and both were treated conservatively. Conclusions: Laparoscopic pancreatic surgery is safe and feasible for patients with benign tumors and cystic lesions. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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15. Effect of prepyloric gastric transection and anastomosis on sphincter of Oddi cyclic motility in conscious dogs.
- Author
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Nabae, Toshinaga, Takahata, Shunichi, Konomi, Hiroyuki, Deng, Zhou-Lu, Yokohata, Kazunori, Chijiiwa, Kazuo, Tanaka, Masao, Nabae, T, Takahata, S, Konomi, H, Deng, Z L, Yokohata, K, Chijiiwa, K, and Tanaka, M
- Subjects
SPHINCTER of Oddi ,GASTRECTOMY ,GASTROINTESTINAL motility - Abstract
Purpose: We previously reported significant changes in sphincter of Oddi cyclic motility after proximal duodenal transection and anastomosis. However, the role of intrinsic myoneural continuity between the antrum and duodenum in this respect is not understood. The aim of this study was to elucidate the effects of prepyloric gastric transection on sphincter of Oddi motility in animals in the conscious state.Methods: Pressures in the bile duct, duodenum, stomach, and sphincter of Oddi and their response to an injection of cholecystokinin-octapeptide were measured in four conscious dogs, with a duodenal cannula, before and after gastric transection and anastomosis 1.5 cm proximal to the pylorus.Results: Gastric transection did not affect the initiation and propagation of the gastroduodenal migration motor complex. Biliary pressure (5.7 +/- 0.15 to 5.5 +/- 0.2 mmHg; P = 0.91), sphincter of Oddi basal pressure (10.6 +/- 0.3 to 10.7 +/- 0.2 mmHg; P = 0.97), and amplitude (26.0 +/- 1.2 to 32.9 +/- 1.7 mmHg; P = 0.304) did not change after gastric transection. Biliary pressure decreased from phase II to phase III of the duodenal migrating motor complex. Cholecystokinin-octapeptide inhibited sphincter of Oddi phasic waves before and after gastric transection.Conclusions: Intrinsic myoneural transection at the prepyloric region does not influence sphincter of Oddi cyclic motility. Preservation of pyloroduodenal myoneural continuity in pylorus-preserving gastrectomy would be beneficial to maintain normal sphincter of Oddi motility. [ABSTRACT FROM AUTHOR]- Published
- 2001
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16. Vector manometric study of the sphincter of Oddi in the dog: functional and morphological correlation.
- Author
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Takeda, Torahiko, Tohma, Hiroki, Yoshida, Junichi, Naritomi, Gen, Konomi, Hiroyuki, Deng, Zhou-Lu, Kinukawa, Naoko, Tanaka, Masao, Takeda, T, Tohma, H, Yoshida, J, Naritomi, G, Konomi, H, Deng, Z L, Kinukawa, N, and Tanaka, M
- Subjects
SPHINCTER of Oddi ,MUSCLE motility ,DOGS ,PHYSIOLOGY - Abstract
The relationship between sphincter of Oddi pressure and the morphological structure of the sphincter was studied in eight dogs prepared with a duodenal cannula. Sphincter of Oddi manometry was performed in awake animals in three directions, ventral, left dorsal, and right dorsal, using a catheter with three radial side holes for recording at one level. The pressure in the ventral direction (26.6+/-1.06 mmHg) (mean+/-SEM) was significantly lower than that in the left and right dorsal directions (30.6+/-1.42 and 31.2+/-1.23 mmHg, respectively). This functional manometric difference in the three directions correlated closely with the morphological structure of the sphincter of Oddi; the sum of the thickness of the sphincter of Oddi muscle and duodenal proper muscle was greater on the dorsal than on the ventral side. To our knowledge, this is the first report of axial asymmetry in sphincter of Oddi pressure. [ABSTRACT FROM AUTHOR]
- Published
- 1998
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17. Effect of local injection of botulinum toxin on sphincter of Oddi cyclic motility in dogs.
- Author
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Wang, Hong-Jiang, Tanaka, Masao, Konomi, Hiroyuki, Toma, Hiroki, Yokohata, Kazunori, Pasricha, Pankaj, Kalloo, Anthony, Wang, H J, Tanaka, M, Konomi, H, Toma, H, Yokohata, K, Pasricha, P J, and Kalloo, A N
- Subjects
DUODENUM physiology ,ANIMAL experimentation ,BIOLOGICAL models ,BOTULINUM toxin ,CHOLECYSTOKININ ,DOGS ,DUODENUM ,GASTROINTESTINAL agents ,GASTROINTESTINAL motility ,MANOMETERS ,MUSCLE relaxants ,PHARMACODYNAMICS - Abstract
To study effects of intrasphincteric injections of botulinum toxin on the sphincter of Oddi cyclic motility and responses to motilin and cholecystokinin, four conscious dogs with duodenal cannula underwent manometry of the common bile duct, sphincter of Oddi, and duodenum. After baseline recording, each dog had intrasphincteric injections of saline or botulinum toxin. The injections of saline had no effect, whereas botulinum toxin significantly reduced mean basal pressure, amplitude, and motility index of the sphincter of Oddi. These effects took place in four to seven days and reached a maximum in seven to 10 days. The basal pressure returned to the baseline level in 28 weeks, but the amplitude and motility index remained low. The pressure parameters of motilin-induced premature phase III-like activity also decreased, but action of cholecystokinin was not affected. These results indicate that the botulinum toxin injections reduce sphincter of Oddi phasic contractile activity for a prolonged period of time. [ABSTRACT FROM AUTHOR]
- Published
- 1998
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18. Change in colonic motility after extrinsic autonomic denervation in dogs.
- Author
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Ishikawa, Mikimasa, Mibu, Ryuichi, Iwamoto, Takuya, Konomi, Hiroyuki, Oohata, Yoshihiro, Tanaka, Masao, Ishikawa, M, Mibu, R, Iwamoto, T, Konomi, H, Oohata, Y, and Tanaka, M
- Abstract
Changes in colonic motility were compared in dogs undergoing autonomic denervation of the paraaortic and presacral (group A), paraaortic (group B), or mesocolonic region (group C), and sham operation (group D). Five bipolar recording electrodes were placed into the seromuscular layer of the colon and rectum. The numbers of continuous electrical response activity and contractile electrical complex after an intragastric olive oil injection were smaller in group A than in the other groups (P < 0.05) from three weeks through six months after denervation. This difference was significant even in the proximal colon. These data suggest that the pelvic plexus may play an important role in colonic motility including the proximal colon. The damage to the plexus did not recover for at least six months after denevation. Pelvic plexus injury may thus be one of possible explanations for the prolonged change in bowel habit after anterior resection of the rectum. [ABSTRACT FROM AUTHOR]
- Published
- 1997
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19. Single oral dose of cisapride accelerates gastric antral emptying in healthy humans: an ultrasonographic study.
- Author
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Takeda, Torahiko, Konomi, Hiroyuki, Naritomi, Gen, Yoshida, Junichi, Matsunaga, Hiroaki, Akazawa, Kouhei, Tanaka, Masao, Takeda, T, Konomi, H, Naritomi, G, Yoshida, J, Matsunaga, H, Akazawa, K, and Tanaka, M
- Abstract
An ultrasonographic study of ten healthy volunteers was carried out to evaluate the effect of cisapride on gastric antral emptying. More than 1 week after the measurement of the baseline emptying rate, cisapride was given at a single oral dose of 5 mg 30 minutes before intake of a balanced liquid test meal (5 ml/kg body weight). To determine the time to half emptying (T1/2), an exponential curve was extrapolated for the elimination phase of the gastroantral sagittal cross-sectional area plotted against time. The T1/2 was reduced by 18.5% after cisapride, from 62.6 +/- 4.3 to 51.0 +/- 4.4 min (P = 0.0284). We conclude that a single oral dose of 5 mg of cisapride significantly accelerates the gastric antral emptying rate in healthy humans. [ABSTRACT FROM AUTHOR]
- Published
- 1996
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20. Biochemical and Immunohistochemical Studies on the Scirrhous Carcinoma of Human Stomacha.
- Author
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NAGAI, Y., SUNADA, H., SANO, J., ONODERA, S., ARAI, K., KONOMI, H., MINAMOTO, T., HATA, R., HORI, H., NAKANISHI, I., KITAOKA, H., and SAKAMOTO, G.
- Published
- 1985
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21. Long-term consequence of endoscopic sphincterotomy for bile duct stones
- Author
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Tanaka, M., Takahata, S., Konomi, H., Matsunaga, H., Yokohata, K., Takeda, T., Utsunomiya, N., and Ikeda, S.
- Abstract
Background: There are many reports of early- and intermediate-term results of endoscopic sphincterotomy. However, few data are available on long-term clinical outcome of endoscopic sphincterotomy for removal of common bile duct stones. Methods: Of 419 patients who underwent endoscopic sphincterotomy, follow-up data were obtained in 410 patients (98%). The period ranged from 1 month to 20 years (average 122 months). Results: Late complications included recurrence of stones (12.3%), acute cholangitis, acute cholecystitis (22% of 32 patients with gallstones, 0% of 88 patients without gallstones), new gallstone formation (6 patients), liver abscess (5 patients), and biliary carcinoma (8 patients). All of the recurrent stones were bilirubinate irrespective of the type of stone at sphincterotomy. Cholangitis and liver abscess occurred in 31% and 11%, respectively, of patients with residual intrahepatic stones but not in patients with complete intrahepatic stone clearance. Conclusions: Late complications occur in a considerable proportion of patients after endoscopic sphincterotomy for the treatment of common bile duct stones, including stone recurrence, acute cholecystitis (which occurs only in patients with gallstones), liver abscess in patients with residual intrahepatic stones, and biliary carcinoma. The fact that the recurrent stones are invariably of the bilirubinate type, irrespective of the type of stones at initial treatment, suggests that bacterial infestation due to ablation of the sphincter mechanism may have a causative role. (Gastrointest Endosc 1998;48:465-9.)
- Published
- 1998
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22. Peptide-specific antibodies identify the alpha 2 chain as the proteoglycan subunit of type IX collagen.
- Author
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Konomi, H, Seyer, J M, Ninomiya, Y, and Olsen, B R
- Abstract
Type IX collagen is a recently characterized product of chondrocytes. The molecules of this collagen are heterotrimers of three genetically distinct polypeptide chains. One of the three chains contains chondroitin and/or dermatan sulfate glycosaminoglycan chains, giving the molecule a proteoglycan character. In fact, Type IX collagen has been identified with the proteoglycan Lt (PG-Lt), first isolated by Noro, A., Kimata, K., Oike, Y., Shinomura, T., Maeda, N., Yano, S., Takahashi, N., and Suzuki, S. (1983) J. Biol. Chem. 258, 9323-9331 from chick embryonic tibia and femur. Based on amino acid sequences predicted from the nucleotide sequences of cDNA and genomic clones specific for two of the chains of Type IX collagen, we have synthesized oligopeptides representing portions of the two chains. In addition, an oligopeptide has been made based on a partial amino acid sequence of the third chain. Antibodies against the synthetic peptides have been generated in rabbits, and the polyclonal sera have allowed identification of the three genetically distinct polypeptide subunits of Type IX collagen. In addition, labeling with [35S]sulfate and treatment with chondroitinase ABC demonstrates that glycosaminoglycan chains are present on the subunit that has been given the designation alpha 2(IX).
- Published
- 1986
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23. Cartilage type IX collagen-proteoglycan contains a large amino-terminal globular domain encoded by multiple exons.
- Author
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Vasios, G, Nishimura, I, Konomi, H, van der Rest, M, Ninomiya, Y, and Olsen, B R
- Abstract
Type IX collagen in cartilage consists of molecules composed of three genetically distinct polypeptide subunits. One of the subunits, alpha 2(IX), contains a covalently attached glycosaminoglycan side chain whereas a second subunit, alpha 1(IX), contains a large noncollagenous, amino-terminal domain called NC4. In this report, we describe for the first time the complete primary structure of this noncollagenous domain, based on cloning and sequencing of cDNA and genomic DNA as well as amino acid sequencing of tryptic peptides. Analysis of genomic clones has also allowed determination of the exon structure of NC4. Our results demonstrate that the noncollagenous, amino-terminal domain of alpha 1(IX) chains contains 266 amino acid residues (including the signal peptide) with 5 cysteinyl residues forming two disulfide bridges. The domain is basic with an estimated pI of 9.7, thus supporting the idea that it may participate in ionic interactions with polyanionic glycosaminoglycans in cartilage. Both the sequence and exon structure of the NC4 domain is unique among collagens and there is no obvious homology with the noncollagenous domains of other types of collagen, including the propeptides of fibrillar collagens.
- Published
- 1988
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24. Localization of type V collagen and type IV collagen in human cornea, lung, and skin. Immunohistochemical evidence by anti-collagen antibodies characterized by immunoelectroblotting
- Author
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Konomi, H., Hayashi, T., Nakayasu, K., and Arima, M.
- Subjects
Adult ,Cornea ,Male ,Histocytochemistry ,Immunologic Techniques ,Humans ,Female ,Collagen ,Middle Aged ,Lung ,Research Article ,Skin - Abstract
Tissue distribution of Type V collagen in comparison with Type IV collagen was investigated by indirect immunofluorescence microscopy. Affinity-purified rat antibodies to Type IV and Type V collagens obtained from human placenta reacted specifically only with the corresponding type of collagen in both native and denatured conformations. In indirect immunofluorescent stainings of human skin, lung, and cornea tissues, Type IV and Type V collagens showed distinct distributions. Type IV collagen was distributed exclusively in basement membrane. However, Type V collagen had two characteristic features; a diffuse distribution in interstitium and the locations on or adjacent to basement membrane. On the basis of these findings and of biochemical characterization of the collagens, it is postulated that Type V collagen is an intermediate collagen, possibly having a function of binding or connecting of interstitial collagen fibrils with membranous collagen networks.
- Published
- 1984
25. Segmental balloon cytology for preoperative localization of in situ pancreatic cancer
- Author
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Tanaka, M., Yokohata, K., Konomi, H., Yamaguchi, K., Chijiiwa, K., and Ohta, M.
- Published
- 1997
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26. Indomethacin Causing Pseudotumor Cerebri in Bartter's Syndrome
- Author
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Imai M, Kamoshita S, Konomi H, Tada H, and Nihei K
- Subjects
medicine.medical_specialty ,Bartter's syndrome ,Pseudotumor cerebri ,business.industry ,medicine ,General Medicine ,medicine.disease ,business ,Dermatology - Published
- 1978
27. Inadvertent pancreatic duct perforation during brushing
- Author
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Sonoda, Y., Kobayashi, K., Kawamoto, M., Konomi, H., Takeda, T., Noshiro, H., and Tanaka, M.
- Published
- 2003
- Full Text
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28. Reappraisal of Safety of Endoscopic Sphincterotomy for Common Bile Duct Stones in the Elderly
- Author
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Deenitchin, G. P., Konomi, H., Kimura, H., and Ogawa, Y.
- Published
- 1995
- Full Text
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29. Potential therapeutic targets discovery by transcriptome analysis of an in vitro human gastric signet ring carcinoma model.
- Author
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Yamaguchi K, Yoshihiro T, Ariyama H, Ito M, Nakano M, Semba Y, Nogami J, Tsuchihashi K, Yamauchi T, Ueno S, Isobe T, Shindo K, Moriyama T, Ohuchida K, Nakamura M, Nagao Y, Ikeda T, Hashizume M, Konomi H, Torisu T, Kitazono T, Kanayama T, Tomita H, Oda Y, Kusaba H, Maeda T, Akashi K, and Baba E
- Subjects
- Cadherins genetics, Cadherins metabolism, Gene Expression Profiling, Germ-Line Mutation, Humans, Carcinoma, Signet Ring Cell genetics, Carcinoma, Signet Ring Cell pathology, Stomach Neoplasms genetics, Stomach Neoplasms pathology
- Abstract
Background: Loss of E-cadherin expression is frequently observed in signet ring carcinoma (SRCC). People with germline mutations in CDH1, which encodes E-cadherin, develop diffuse gastric cancer at a higher rate. Loss of E-cadherin expression is thus assumed to trigger oncogenic development., Methods: To investigate novel therapeutic targets for gastric SRCC, we engineered an E-cadherin-deficient SRCC model in vitro using a human gastric organoid (hGO) with CDH1 knockout (KO)., Results: CDH1 KO hGO cells demonstrated distinctive morphological changes similar to SRCC and high cell motility. RNA-sequencing revealed up-regulation of matrix metalloproteinase (MMP) genes in CDH1 KO hGO cells compared to wild type. MMP inhibitors suppressed cell motility of CDH1 KO hGO cells and SRCC cell lines in vitro. Immunofluorescent analysis with 95 clinical gastric cancer tissues revealed that MMP-3 was specifically abundant in E-cadherin-aberrant SRCC. In addition, CXCR4 molecules translocated onto the cell membrane after CDH1 KO. Addition of CXCL12, a ligand of CXCR4, to the culture medium prolonged cell survival of CDH1 KO hGO cells and was abolished by the inhibitor, AMD3100. In clinical SRCC samples, CXCL12-secreting fibroblasts showed marked infiltration into the cancer area., Conclusions: E-cadherin deficient SRCCs might gain cell motility through upregulation of MMPs. CXCL12-positive cancer-associated fibroblasts could serve to maintain cancer-cell survival as a niche. MMPs and the CXCL12/CXCR4 axis represent promising candidates as novel therapeutic targets for E-cadherin-deficient SRCC., (© 2022. The Author(s) under exclusive licence to The International Gastric Cancer Association and The Japanese Gastric Cancer Association.)
- Published
- 2022
- Full Text
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30. Laparoscopic high anterior resection for triple colorectal cancers with persistent ascending and descending mesocolons: A case report.
- Author
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Hisano K, Ueki T, Kono H, Ikenaga N, Maeyama R, Konomi H, Okido M, Yamashita N, Motoshita J, and Ichimiya H
- Subjects
- Adenocarcinoma pathology, Aged, 80 and over, Colorectal Neoplasms pathology, Humans, Male, Adenocarcinoma surgery, Colon, Ascending abnormalities, Colon, Descending abnormalities, Colorectal Neoplasms surgery, Laparoscopy, Mesocolon abnormalities
- Abstract
Persistent mesocolon is an embryological anomaly of the colon resulting from failure of the primitive dorsal mesocolon to fuse with the parietal peritoneum. We herein present a case of laparoscopic high anterior resection for triple colorectal cancers with persistent ascending and descending mesocolons and a right-bound inferior mesenteric artery. Preoperative 3-D CT demonstrated that the sigmoid colon had shifted to the right abdomen and was located under the ascending colon. Moreover, the inferior mesenteric artery and vein traveled toward the right abdomen accompanied by the mesentery of the descending colon. Adhesiolysis between the ascending and sigmoid colon was initially performed, and the sigmoid colon was placed in its normal position. The inferior mesenteric artery was then divided with lymph node dissection using a medial approach, and high anterior resection was completed. An understanding of the anatomical characteristics of persistent mesocolon is important to ensure safe laparoscopic surgery., (© 2018 Japan Society for Endoscopic Surgery, Asia Endosurgery Task Force and John Wiley & Sons Australia, Ltd.)
- Published
- 2019
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31. Stage IV gastric cancer successfully treated by multidisciplinary therapy including chemotherapy, immunotherapy, and surgery: a case report.
- Author
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Kawamoto M, Onishi H, Koya N, Konomi H, Mitsugi K, Tanaka R, Motoshita J, Morisaki T, and Nakamura M
- Abstract
Background: The prognosis of stage IV gastric cancer (GC) still remains unfavorable. Multidisciplinary approaches should therefore be considered to improve the survival of patients with stage IV GC. We report here a case of primary GC with potentially unresectable metastasis, successfully treated by a multidisciplinary approach including chemotherapy, immunotherapy, and surgery., Case Presentation: A 74-year-old man presented with multiple left neck masses. Abdominal computed tomography showed a thickened gastric wall and multiple lymphadenopathies including left supraclavicular lymph node. Gastroenterological endoscopy revealed tumor lesions in the gastric cardia. Tumor biopsy indicated a pathological diagnosis of poorly differentiated adenocarcinoma. Open left cervical lymph node biopsy showed histological features identical with the gastric tumor, indicating left clavicle lymph node metastasis of GC. After 2 years of chemo-immunotherapy with S-1/CDDP, paclitaxel, and cytokine-activated killer cells, lesions other than the stomach lesion had regressed to undetectable on imaging studies. The patient then underwent laparoscopy-assisted total gastrectomy with Roux-en-Y reconstruction followed by adjuvant chemo-immunotherapy with paclitaxel and S-1 for 1 year, and immunotherapy with tumor lysate-pulsed dendritic cell-activated killer cells for 5 years. The patient remained well after 5 years and 6 months of follow-up, with no signs of recurrence., Conclusion: Therapeutic combinations including immunotherapy may thus allow surgery to be performed in patients previously considered unsuitable for surgical intervention, potentially leading to a clinical cure, as in the current case.
- Published
- 2017
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32. A Feasibility Study of Neoadjuvant XELOX Without Radiotherapy for Locally Advanced Lower Rectal Cancer.
- Author
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Ueki T, Manabe T, Inoue S, Ienaga J, Yamanaka N, Egami T, Ishikawa M, Konomi H, Ikubo A, Nagayoshi K, Nakamura M, and Tanaka M
- Subjects
- Adult, Aged, Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols adverse effects, Capecitabine, Carcinoma pathology, Carcinoma surgery, Chemotherapy, Adjuvant, Deoxycytidine administration & dosage, Deoxycytidine adverse effects, Feasibility Studies, Female, Fluorouracil administration & dosage, Fluorouracil adverse effects, Humans, Japan, Male, Middle Aged, Neoplasm Staging, Neoplasm, Residual, Oxaloacetates, Pilot Projects, Prospective Studies, Rectal Neoplasms pathology, Rectal Neoplasms surgery, Time Factors, Treatment Outcome, Young Adult, Antineoplastic Combined Chemotherapy Protocols administration & dosage, Carcinoma drug therapy, Deoxycytidine analogs & derivatives, Fluorouracil analogs & derivatives, Neoadjuvant Therapy, Rectal Neoplasms drug therapy
- Abstract
Aim: This study was planned to evaluate the efficacy and safety of preoperative capecitabine and oxaliplatin (XELOX) without radiation in patients with locally advanced lower rectal cancer., Patients and Methods: Patients with clinical stage II/III lower rectal cancer underwent three cycles of XELOX followed by radical surgery. The primary end-point was the R0 resection rate., Results: Thirty-one patients were recruited between February 2012 and August 2014. The completion rate of neoadjuvant chemotherapy was 96.5% among the 29 patients who received it; the remaining two refused chemotherapy and underwent immediate surgery. Grade 3-4 adverse events occurred in nine patients (31%). All 29 patients who received chemotherapy underwent radical resection. The R0 resection rate was 96.5% among these 29 patients. Pathological complete responses were achieved in three patients (10.3%) and downstaging occurred in 13 (44.8%)., Conclusion: This pilot study found that neoadjuvant XELOX for locally advanced lower rectal cancer is feasible and safe. This neoadjuvant treatment improved resection margin status., (Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Published
- 2016
33. Metastatic breast carcinoma simulating linitis plastica of the colon: report of a case.
- Author
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Okido M, Seo M, Hamada Y, Kurihara S, Matsumoto K, Konomi H, Kato M, and Ichimiya H
- Subjects
- Biomarkers, Tumor analysis, Breast Neoplasms surgery, Colonic Neoplasms diagnostic imaging, Colonic Neoplasms surgery, Diagnosis, Differential, Female, Humans, Immunohistochemistry, Linitis Plastica diagnosis, Lymph Node Excision, Mastectomy, Middle Aged, Neoplasm Invasiveness, Radiography, Breast Neoplasms pathology, Colonic Neoplasms secondary
- Abstract
A 48-year-old woman developed a mobile abdominal mass in the course of treatment for recurrent breast cancer. Imaging studies indicated linitis plastica of the colon. She underwent surgery because of the stenosis of the transverse colon. An examination of the resected specimen revealed a segmental stricture, thickening of the entire wall, and a granular mucosa resembling cobblestones. Microscopic findings of the colon lesion were very similar to those of her primary, invasive lobular carcinoma of the breast. Atypical cells showed immunoreactivity for cytokeratin-7, but not for cytokeratin-20. These findings suggested that the lesion of the colon was a colonic metastasis of breast cancer. Metastatic gastrointestinal diseases originating from breast carcinoma are unusual, and colonic metastases are especially rare. Although colon cancer may occur in patients with a history of breast cancer, metastatic colon cancer should be suspected if linitis plastica is detected.
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- 2011
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34. Twist, a novel oncogene, is upregulated in pancreatic cancer: clinical implication of Twist expression in pancreatic juice.
- Author
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Ohuchida K, Mizumoto K, Ohhashi S, Yamaguchi H, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- Adenocarcinoma, Mucinous genetics, Adenocarcinoma, Mucinous metabolism, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Carcinoma, Pancreatic Ductal genetics, Carcinoma, Pancreatic Ductal metabolism, Carcinoma, Papillary genetics, Carcinoma, Papillary metabolism, Cells, Cultured, Epithelial Cells metabolism, Humans, Pancreas metabolism, Pancreas pathology, Pancreatic Neoplasms metabolism, Pancreatitis genetics, Pancreatitis metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation, Gene Expression Regulation, Neoplastic, Nuclear Proteins genetics, Pancreatic Juice physiology, Pancreatic Neoplasms genetics, Twist-Related Protein 1 genetics
- Abstract
Despite evidence that Twist, a highly conserved basic helix-loop-helix transcription factor, is a novel oncogene, there are no reports describing Twist expression in pancreatic cancer. Intraductal papillary mucinous neoplasm (IPMN) and pancreatic intraepithelial neoplasia (PanIN) are precursor lesions of pancreatic cancer. To clarify involvement of Twist expression in pancreatic cancer, we used quantitative reverse transcription-polymerase chain reaction and examined Twist expression in pancreatic cancer, IPMN, and non-neoplastic pancreas using bulk tissues (11 cancers, 18 IPMNs, and 15 non-neoplastic pancreata), microdissected cells (cancer from 22 sections, IPMN from 19 sections, PanIN from 6 sections, and pancreatitis-affected epithelial cells from 14 sections), and pancreatic juice (16 from cancer, 28 from IPMN, and 17 from pancreatitis). Twist expression differed significantly between cancer and IPMN bulk tissues (p < 0.0001) but not between cancer and non-neoplastic tissues. Twist expressions differed significantly between microdissected cancer cells, IPMN cells, and pancreatitis-affected cells (all comparisons, p < 0.017). PanIN cells expressed significantly lower levels of Twist than did IDC cells (p = 0.016). Twist expression differed significantly between cancer and IPMN juice samples (p = 0.0002) but not between cancer and pancreatitis juice samples. Receiver operation characteristic curve analyses revealed that measurement of Twist was more useful for discriminating cancer from IPMN than from chronic pancreatitis (p = 0.009). Our results suggest that Twist is involved in tumor progression of pancreatic cancer and that measurement of Twist in pancreatic juice may be useful to differentiate pancreatic cancer from nonmalignant neoplasms such as IPMN., ((c) 2007 Wiley-Liss, Inc.)
- Published
- 2007
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35. S100A6 is increased in a stepwise manner during pancreatic carcinogenesis: clinical value of expression analysis in 98 pancreatic juice samples.
- Author
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Ohuchida K, Mizumoto K, Yu J, Yamaguchi H, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- Aged, Biomarkers, Tumor metabolism, Chi-Square Distribution, Female, Humans, Male, Middle Aged, Pancreatic Neoplasms pathology, RNA, Neoplasm analysis, ROC Curve, Reverse Transcriptase Polymerase Chain Reaction, S100 Calcium Binding Protein A6, Statistics, Nonparametric, Cell Cycle Proteins metabolism, Pancreatic Juice chemistry, Pancreatic Neoplasms metabolism, S100 Proteins metabolism
- Abstract
There are few reports describing the diagnostic significance of S100A6 expression in clinical samples obtained from patients with pancreatic disease. In the present study, we measured S100A6 expression in pancreatic tissues and juice to evaluate its involvement in pancreatic carcinogenesis. We did quantitative real-time reverse transcription-PCR to measure mRNA expression in microdissected cells and pancreatic juice samples. Microdissected invasive ductal carcinoma and intraductal papillary mucinous neoplasm (IPMN) cells expressed significantly higher levels of S100A6 than did microdissected pancreatitis-affected epithelial and normal cells (all comparison; P < 0.008). Median levels of S100A6 in invasive ductal carcinoma were higher than those in IPMN, and those in pancreatitis-affected epithelial cells tended to be higher than those in normal cells, although these differences were not statistically significant. In analyses of pancreatic juice, IPMN and pancreatic cancer samples expressed significantly higher levels of S100A6 than did chronic pancreatitis samples (both; P < 0.017), but levels in pancreatic cancer and IPMN samples did not differ form each other. Receiver operating characteristic (ROC) curve analysis revealed that measurement of S100A6 was useful for discriminating cancer (area under the ROC curve, 0.864) or IPMN (area under the ROC curve, 0.749) from chronic pancreatitis. The present data suggest that expression of S100A6 is increased in a stepwise manner during pancreatic carcinogenesis and may be a biomarker for evaluating malignant potential. Measurement of S100A6 in pancreatic juice may be useful to detect early pancreatic cancer or identify individuals with high-risk lesions that may progress to pancreatic cancer.
- Published
- 2007
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36. Pylorus-preserving pancreatoduodenectomy: preoperative pancreatic function and outcome.
- Author
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Ohuchida J, Chijiiwa K, Ohtsuka T, Konomi H, and Tanaka M
- Subjects
- Aged, Aged, 80 and over, Body Weight, Female, Gastric Emptying, Humans, Male, Middle Aged, Pancreas, Exocrine physiology, Quality of Life, Treatment Outcome, Digestive System Diseases surgery, Pancreas physiology, Pancreaticoduodenectomy adverse effects, Pancreaticoduodenectomy methods, Pylorus surgery
- Abstract
Background/aims: To investigate the effects of preoperative pancreatic function on gastric emptying, body weight, and quality of life after pylorus-preserving pancreatoduodenectomy., Methodology: Thirty-one patients who underwent pylorus-preserving pancreatoduodenectomy were divided into 2 groups according to preoperative pancreatic exocrine and endocrine function (normal vs. abnormal). Gastric emptying, body weight, and quality of life were evaluated before surgery, 1-2 months after surgery (short-term), and 6-12 months after surgery (long-term)., Results: Short-term body weight was significantly decreased in comparison to preoperative body weight regardless of preoperative exocrine and endocrine pancreatic function. Body weight returned to the preoperative level by 12 months after surgery in patients with normal preoperative pancreatic function but not in patients with abnormal pancreatic function. In both groups, gastric emptying was delayed at 1-2 months after surgery and then returned to the preoperative value by 12 months. Short-term quality of life did not differ from preoperative quality of life in either group, but long-term quality of life improved to beyond the preoperative level in both groups., Conclusions: Preoperative pancreatic function appears to significantly influence long-term body weight after pylorus-preserving pancreatoduodenectomy.
- Published
- 2007
37. Autoimmune pancreatitis diagnosed after pancreatoduodenectomy and successfully treated with low-dose steroid.
- Author
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Watanabe M, Yamaguchi K, Kobayashi K, Konomi H, Nakamura M, Mizumoto K, Tsuneyoshi M, and Tanaka M
- Subjects
- Administration, Oral, Aged, Endosonography, Female, Humans, Immunoglobulin G blood, Magnetic Resonance Imaging, Pancreaticoduodenectomy methods, Recurrence, Treatment Outcome, Ultrasonography, Interventional, Autoimmune Diseases diagnosis, Autoimmune Diseases drug therapy, Autoimmune Diseases surgery, Glucocorticoids therapeutic use, Pancreatitis diagnosis, Pancreatitis drug therapy, Pancreatitis surgery, Prednisolone therapeutic use
- Abstract
A 69-year-old woman presented with obstructive jaundice and a 30-mm hypoechoic mass in the pancreatic head on ultrasonography. Magnetic resonance imaging (MRI) revealed enlargement of the pancreatic head with dilatation of the upstream main pancreatic duct and no dilatation of the proximal biliary tree. Endoscopic retrograde pancreatography showed a localized irregular narrowing of the main pancreatic duct in the head of the pancreas. Pylorus-preserving pancreatoduodenectomy (PPPD) was performed under the diagnosis of pancreatic head cancer. Histopathological examination showed fibrosis with lymphoplasmacytic infiltration, suggesting the diagnosis of autoimmune pancreatitis (AIP). Serum IgG concentration was within normal limits immediately after the operation but was elevated 4 months later, when MRI showed enlargement of the remnant pancreas, with a peripheral rim of low intensity. Oral administration of prednisolone was initiated, at a dose of 5 mg/day. The serum IgG concentration declined and MRI showed improvement of the pancreatic enlargement and the disappearance of the peripheral rim. AIP has not relapsed for 1 year so far, with the patient being kept on 5 mg/day prednisolone. This communication reports a patient with AIP showing an interesting clinical course.
- Published
- 2007
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38. Long-term results of treatment for pancreaticobiliary maljunction without bile duct dilatation.
- Author
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Ohuchida J, Chijiiwa K, Hiyoshi M, Kobayashi K, Konomi H, and Tanaka M
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Anastomosis, Roux-en-Y, Chi-Square Distribution, Child, Child, Preschool, Cholangiopancreatography, Endoscopic Retrograde, Cholangiopancreatography, Magnetic Resonance, Cholecystectomy, Laparoscopic, Dilatation, Pathologic, Female, Humans, Infant, Male, Middle Aged, Retrospective Studies, Treatment Outcome, Bile Ducts abnormalities, Bile Ducts surgery, Biliary Tract Surgical Procedures, Gallbladder Neoplasms surgery, Pancreatic Ducts abnormalities, Pancreatic Ducts surgery, Pancreatic Neoplasms surgery
- Abstract
Hypothesis: Resection of the gallbladder together with the dilated bile duct is the preferred treatment for pancreaticobiliary maljunction (PBM) with bile duct dilatation, whereas this treatment for PBM without bile duct dilatation is still controversial., Design: Retrospective study of 196 patients from January 1979 to November 2004., Setting: Two university hospitals., Patients: One hundred ninety-six patients with PBM, 152 (78%) with and 44 (22%) without bile duct dilatation, formed the basis of this study., Main Outcome Measures: The effects of cholecystectomy on long-term results in the patients without bile duct dilatation., Results: Significant differences were observed in patients without bile duct dilatation: patients were older, carcinoma of the gallbladder was more prevalent (19 patients [43.2%] without dilatation vs 9 patients [5.9%] with dilatation), and pancreatic cancer and pancreatitis were also more frequent. Most of their gallbladder carcinomas were found at stage IV (63%). The outcome was very poor in stage IV, whereas 5 patients in stage I and II lived for more than 5 years after surgery. Of the 44 patients without bile duct dilatation, 23 with carcinoma of the gallbladder or pancreas died and the other 2 were lost to follow-up. The remaining 19 patients were alive at the study's conclusion after cholecystectomy without bile duct resection. None of them had bile duct carcinoma at the time of surgery or during the mean follow-up period of 9 years after surgery., Conclusions: Prophylactic cholecystectomy without bile duct resection is the best treatment option for patients with PBM without bile duct dilatation. Possible association of gallbladder carcinoma should be kept in mind at the time of treatment of patients with PBM when the bile duct is not dilated.
- Published
- 2006
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39. S100P is an early developmental marker of pancreatic carcinogenesis.
- Author
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Ohuchida K, Mizumoto K, Egami T, Yamaguchi H, Fujii K, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- Adenocarcinoma, Mucinous metabolism, Carcinoma, Pancreatic Ductal metabolism, Carcinoma, Papillary metabolism, Cell Culture Techniques, Cell Line, Tumor, Disease Progression, Fibroblasts metabolism, Humans, Pancreas metabolism, Pancreatic Juice metabolism, Pancreatic Neoplasms pathology, RNA, Messenger metabolism, Biomarkers metabolism, Calcium-Binding Proteins metabolism, Carcinoma metabolism, Neoplasm Proteins metabolism, Pancreatic Neoplasms metabolism
- Abstract
Purpose: Our goal was to clarify the involvement and clinical significance of S100P in pancreatic carcinogenesis., Experimental Design: We examined S100P expression in 45 bulk pancreatic tissues; in microdissected cells, including invasive ductal carcinoma (IDC) cells (20 sections), pancreatic intraepithelial neoplasia (PanIN) cells (12 sections), intraductal papillary mucinous neoplasm (IPMN) cells (19 sections), and normal epithelial cells (11 sections); and in pancreatic juice samples from 99 patients with pancreatic diseases (32 cancer, 35 IPMN, and 32 chronic pancreatitis samples). We used quantitative real-time reverse transcription-PCR with gene-specific priming to measure S100P in these various types of samples., Results: In bulk tissue analyses, pancreatic cancer and IPMN expressed significantly higher levels of S100P than did nonneoplastic pancreas (P<0.017 and P=0.0013, respectively). Microdissection analyses revealed that IPMN expressed significantly higher levels of S100P than did IDC (P<0.0001) and PanIN (P=0.0031), although S100P expression did not differ between IDC and PanIN (P=0.077). In pancreatic juice analyses, cancer and IPMN juice expressed significantly higher levels of S100P than did pancreatitis juice (both P<0.0001). Receiver operating characteristic curve analyses revealed that measurement of S100P in pancreatic juice was useful for discriminating neoplastic disease from chronic pancreatitis (area under the curve=0.837; 95% confidence interval, 0.749-0.903)., Conclusion: S100P may be an early developmental marker of pancreatic carcinogenesis, and measurement of S100P in pancreatic juice may be useful for early detection of pancreatic cancer or screening of early pancreatic carcinogenesis.
- Published
- 2006
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40. S100A11, a putative tumor suppressor gene, is overexpressed in pancreatic carcinogenesis.
- Author
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Ohuchida K, Mizumoto K, Ohhashi S, Yamaguchi H, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- Carcinoma, Pancreatic Ductal metabolism, Cell Culture Techniques, Cell Line, Tumor, Humans, Pancreas metabolism, Pancreatic Juice metabolism, Pancreatic Neoplasms pathology, RNA, Messenger metabolism, Carcinoma metabolism, Genes, Tumor Suppressor physiology, Pancreatic Neoplasms metabolism, S100 Proteins metabolism, S100 Proteins physiology
- Abstract
Purpose: Recent microarray analyses revealed that expression of S100A11 is up-regulated in pancreatic cancer. The aim of the present study was to evaluate the association of S100A11 with pancreatic carcinogenesis., Experimental Design: We measured S100A11 mRNA expression in various clinical samples related to pancreatic cancer and its precursor lesions, intraductal papillary mucinous neoplasm (IPMN) and pancreatic intraepithelial neoplasia, by quantitative reverse transcription-PCR., Results: Levels of S100A11 were significantly higher in pancreatic cancer (n=22) and IPMN (n=18) bulk tissues than in nonneoplastic bulk tissues (n=22; P<0.0001 for both). Levels of S100A11 did not differ between pancreatic cancer and IPMN bulk tissues. In microdissection analyses, however, IPMN cells (n=21) expressed significantly higher levels of S100A11 than did cancer cells (n=23; P=0.003). The median level of S100A11 expression was higher in pancreatic intraepithelial neoplasia cells (n=6) than in cancer cells. In pancreatic juice analyses, cancer-related (n=24; P=0.004) and IPMN-related (n=18; P=0.001) juice expressed significantly higher levels of S100A11 than did chronic pancreatitis-related juice (n=23)., Conclusions: The present data suggest that expression of S100A11, a putative tumor suppressor gene, is increased in the early stage of pancreatic carcinogenesis and decreased during subsequent progression to cancer. Analysis of the S100A11 level in pancreatic juice may be an effective tool for screening of patients with high-risk lesions that could progress to pancreatic cancer or detecting early-stage pancreatic cancer.
- Published
- 2006
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41. Quantitative analysis of human telomerase reverse transcriptase in pancreatic cancer.
- Author
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Ohuchida K, Mizumoto K, Yamada D, Yamaguchi H, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- DNA-Binding Proteins genetics, Gene Expression Profiling, Gene Expression Regulation, Enzymologic genetics, Gene Expression Regulation, Neoplastic genetics, Humans, Pancreatic Neoplasms genetics, RNA, Messenger genetics, Telomerase genetics, DNA-Binding Proteins analysis, Pancreatic Neoplasms enzymology, Telomerase analysis
- Abstract
Although telomerase activity is a promising diagnostic marker, clinical introduction of this marker for cancer diagnosis is still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one of the subunits of telomerase, is also a promising diagnostic marker. In the present study, we did large-scale analysis of 88 pancreatic juice samples to determine the feasibility of quantitative analysis of hTERT mRNA for diagnosis of pancreatic cancer. We found significant differences in hTERT expression among carcinoma-derived, intraductal papillary mucinous neoplasm (IPMN)-derived, and chronic pancreatitis-derived juice samples. Results showed that quantitative analyses of hTERT mRNAs are more useful in discriminating carcinoma from IPMN than from chronic pancreatitis. When the specificity was set at 100%, the sensitivity for differentiation between carcinoma and IPMN was 43.5%, whereas the sensitivity of cytologic examination was 22.0%. There were significant differences in hTERT expression among carcinoma cells, IPMN cells, and normal ductal cells isolated from pancreatic tissues by microdissection. Lymphocytes and hyperplastic epithelial cells isolated from tissues with the histologic appearance of pancreatitis showed various expression levels of hTERT. Our results suggest that quantitative analysis of hTERT mRNA in pancreatic juice is advantageous over cytologic analysis for differentiation between carcinoma and IPMN but probably not for differentiation between carcinoma and chronic pancreatitis.
- Published
- 2006
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42. [A case of primary gastric malignant lymphoma perforated immediately after administration of chemotherapy].
- Author
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Yoshino S, Nakamura S, Matsumoto T, Konomi H, Hirahashi M, Yao T, and Iida M
- Subjects
- Aged, Cyclophosphamide adverse effects, Doxorubicin adverse effects, Doxorubicin analogs & derivatives, Humans, Male, Prednisolone adverse effects, Vincristine adverse effects, Antineoplastic Combined Chemotherapy Protocols adverse effects, Lymphoma, B-Cell complications, Lymphoma, B-Cell drug therapy, Lymphoma, Large B-Cell, Diffuse complications, Lymphoma, Large B-Cell, Diffuse drug therapy, Stomach Diseases chemically induced, Stomach Neoplasms complications, Stomach Neoplasms drug therapy
- Abstract
A 77-year-old man was admitted to our hospital with complaints of abdominal pain and body weight loss. Esophagogastroduodenoscopy on admission revealed large ulcerative tumor in the entire region from upper to lower body of the stomach. We diagnosed gastric malignant lymphoma, diffuse large B-cell type and determined stage IV according to the Lugano International Conference classification. Due to gastric perforation occurred on day 4 of chemotherapy, total gastrectomy and partial resection of the transverse colon were performed. Complete remission was achieved by 2 cycles of postoperative chemotherapy together with rituximab.
- Published
- 2006
43. Quantitative analysis of MUC1 and MUC5AC mRNA in pancreatic juice for preoperative diagnosis of pancreatic cancer.
- Author
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Ohuchida K, Mizumoto K, Yamada D, Fujii K, Ishikawa N, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- Biomarkers, Tumor analysis, Case-Control Studies, Diagnosis, Differential, Humans, Immunohistochemistry, Mucin 5AC, Mucin-1 biosynthesis, Mucin-1 genetics, Mucins biosynthesis, Mucins genetics, Pancreatic Juice chemistry, Polymerase Chain Reaction, Preoperative Care, RNA, Messenger analysis, Tumor Cells, Cultured, Mucin-1 analysis, Mucins analysis, Pancreatic Neoplasms diagnosis
- Abstract
Pancreatic juice is a promising type of diagnostic sample for pancreatic cancer, and members of the mucin (MUC) family are diagnostic candidates. To evaluate the utility of MUC family members as diagnostic markers, we measured MUC mRNA expression in pancreatic tissues and pancreatic juice obtained from patients with different pancreatic diseases as well as in pancreatic cancer cell lines by real-time PCR. Furthermore, to support the possibility of early diagnosis by quantification of MUC1 and MUC5AC, immunohistochemistry and microdissection-based quantitative analysis of mRNA were carried out. There was no significant correlation between MUC1 and MUC5AC expression in cell lines. When beta-actin was used as a reference gene, median MUC1 and MUC5AC mRNA expression levels were remarkably greater in tumoral tissues than in non-tumoral tissues, but median MUC4 and MUC6 mRNA expression levels were not. Receiver operating characteristic curve analysis showed that quantitative analysis of MUC1 and MUC5AC mRNA in pancreatic juice is better diagnostic modality than that of MUC4 and MUC6 mRNA. Immunohistochemistry showed that MUC1 and MUC5AC were highly expressed in invasive ductal carcinomas (IDC) and moderately expressed in high-grade pancreatic intraepithelial neoplasia (PanIN); no staining was observed in normal ducts. Analysis of cells isolated by microdissection showed stepwise upregulation of MUC1 and MUC5AC in the development of high-grade PanIN to IDC. Our results suggest that MUC1 and MUC5AC are upregulated stepwise in pancreatic carcinogenesis and that quantitative assessment of MUC1 and MUC5AC mRNA in pancreatic juice has high potential for preoperative diagnosis of pancreatic cancer., (Copyright 2005 Wiley-Liss, Inc.)
- Published
- 2006
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44. Type of gastrointestinal reconstruction affects postoperative recovery after pancreatic head resection.
- Author
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Kawamoto M, Konomi H, Kobayashi K, Shimizu S, Yamaguchi K, and Tanaka M
- Subjects
- Adult, Aged, Aged, 80 and over, Duodenum surgery, Female, Gastrointestinal Motility, Gastrostomy, Humans, Jejunum surgery, Male, Manometry, Middle Aged, Plastic Surgery Procedures, Treatment Outcome, Digestive System Surgical Procedures methods, Pancreatic Neoplasms surgery, Pancreaticoduodenectomy methods
- Abstract
Background/purpose: The postoperative recovery of gastric motility with various reconstructions after pancreatic head resection has been reported. However, little is known about this recovery after pancreatic head resection with segmental duodenectomy (PHRSD). Some have attributed gastric stasis after pylorus-preserving pancreatoduodenectomy (PPPD) to tube gastrostomy, but its effect on gastric motility has not been investigated. In this study, the postoperative recovery after PHRSD and PPPD, and gastric motility with and without gastrostomy after PPPD were investigated., Methods: We analyzed the first appearance of gastric phase III motility, postoperative systemic status, and body weight (BW; n = 32). The Imanaga PPPD and PHRSD were compared because the procedures differ only in the length of the remaining duodenum. Traverso and Roux-en-Y PPPDs were compared because the two procedures are similar except for the creation of gastrostomy., Results: (1) Times to first appearance of gastric phase III motility and BW recovery were significantly better after PHRSD than after the Imanaga PPPD (P < 0.05). (2) Times to first gastric phase III motility and resumption of a regular diet as well as periods of gastric sump tube use and postoperative hospital stay were significantly shorter after the Roux-en-Y than after the Traverso PPPD (P < 0.05)., Conclusions: Preservation of as long a portion of the duodenum as possible, the choice of a Roux-en-Y duodenojejunostomy, and the avoidance of peritoneal fixation of the gastric wall may be factors that improve the recovery of gastric motility and BW after pancreatic head resection.
- Published
- 2006
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45. [Japanese guidelines for treatment of pancreatic cancer based on evidence-based medicine].
- Author
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Yamaguchi K, Watanabe M, Nakamura M, Konomi H, and Tanaka M
- Subjects
- Algorithms, Humans, Japan, Evidence-Based Medicine, Pancreatic Neoplasms therapy, Practice Guidelines as Topic
- Published
- 2006
46. [Laparoscopic approach for gallbladder cancer].
- Author
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Tanabe R, Shimizu S, Konomi H, Nagai E, Yamaguchi K, and Tanaka M
- Subjects
- Diagnostic Imaging, Gallbladder Neoplasms diagnosis, Gallbladder Neoplasms pathology, Humans, Monitoring, Intraoperative, Neoplasm Staging, Postoperative Complications, Cholecystectomy, Laparoscopic methods, Gallbladder Neoplasms surgery
- Published
- 2006
47. [Intraductal papillary-mucinous neoplasm of the pancreas].
- Author
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Yamaguchi K, Watanabe M, Nakamura M, Konomi H, and Tanaka M
- Subjects
- Adenocarcinoma, Mucinous diagnosis, Adenocarcinoma, Mucinous therapy, Adenocarcinoma, Papillary diagnosis, Adenocarcinoma, Papillary therapy, Carcinoma, Pancreatic Ductal diagnosis, Carcinoma, Pancreatic Ductal therapy, Humans, Internationality, Pancreatic Neoplasms diagnosis, Pancreatic Neoplasms therapy, Practice Guidelines as Topic, Adenocarcinoma, Mucinous pathology, Adenocarcinoma, Papillary pathology, Carcinoma, Pancreatic Ductal pathology, Pancreatic Neoplasms pathology
- Published
- 2006
48. The role of S100A6 in pancreatic cancer development and its clinical implication as a diagnostic marker and therapeutic target.
- Author
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Ohuchida K, Mizumoto K, Ishikawa N, Fujii K, Konomi H, Nagai E, Yamaguchi K, Tsuneyoshi M, and Tanaka M
- Subjects
- Cell Cycle Proteins biosynthesis, Cell Line, Tumor, Cell Proliferation, Chemotactic Factors biosynthesis, Down-Regulation, Humans, Immunohistochemistry, Oligonucleotide Array Sequence Analysis, Oligonucleotides chemistry, Pancreas metabolism, Pancreatic Neoplasms diagnosis, RNA Interference, RNA, Messenger metabolism, RNA, Small Interfering metabolism, S100 Calcium Binding Protein A6, S100 Proteins biosynthesis, Time Factors, Up-Regulation, Biomarkers, Tumor, Cell Cycle Proteins metabolism, Gene Expression Regulation, Neoplastic, Pancreatic Neoplasms metabolism, Pancreatic Neoplasms pathology, S100 Proteins metabolism
- Abstract
Recent microarray analyses showed that the S100 family contains members that are candidate diagnostic markers or therapeutic targets. In the present study, to evaluate the involvement of S100A6 in pancreatic cancer and its clinical usefulness for diagnosis, we examined S100A6 mRNA expression in pancreatic tissues and pancreatic juice from patients with different pancreatic diseases. To investigate the role of S100A6 in carcinogenesis of pancreatic cancer and the potential of S100A6 as a diagnostic marker for early detection of pancreatic cancer, we did immunohistochemistry and microdissection-based mRNA analysis of pancreatic normal ducts, pancreatic intraepithelial neoplasias, and invasive ductal carcinomas. We also used in vitro experiments and microarray analysis with RNA interference to evaluate the functional role of S100A6 and its potential as a therapeutic target for pancreatic cancer. S100A6 mRNA levels were significantly higher in carcinoma specimens than in nonneoplastic tissues. In pancreatic juice, there was a significant difference in S100A6 expression between patients with carcinoma and those with nonneoplastic disease. Receiver operating characteristic curves revealed that S100A6 might be a useful marker for diagnosis of pancreatic cancer. Immunohistochemistry and microdissection-based analysis showed differential expression of S100A6 among normal ducts, pancreatic intraepithelial neoplasias, and invasive ductal carcinomas. In vitro data showed that inhibition of S100A6 decreased proliferation and invasiveness of cancer cells, and these findings were supported by microarray data. Our present results suggest that quantitation of S100A6 mRNA is a promising tool for diagnosis of pancreatic cancer, and that S100A6 may be a promising therapeutic target for pancreatic cancer.
- Published
- 2005
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49. Total pancreatectomy for intraductal papillary-mucinous tumor of the pancreas: reappraisal of total pancreatectomy.
- Author
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Yamaguchi K, Konomi H, Kobayashi K, Ogura Y, Sonoda Y, Kawamoto M, Nakano K, and Tanaka M
- Subjects
- Adenocarcinoma diagnosis, Adult, Aged, Carcinoma, Pancreatic Ductal diagnosis, Cholangiopancreatography, Endoscopic Retrograde, Cholangiopancreatography, Magnetic Resonance, Dilatation, Pathologic, Female, Humans, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Invasiveness, Pancreatic Ducts pathology, Pancreatic Neoplasms diagnosis, Carcinoma, Pancreatic Ductal surgery, Pancreatectomy methods, Pancreatic Neoplasms surgery
- Abstract
Background/aims: Total pancreatectomy is rarely performed as the treatment of pancreatic carcinoma because of markedly impaired quality of life and poor prognosis. Intraductal papillary-mucinous tumor (IPMT) of the pancreas is characterized by extensive intraductal spread and favorable outcome even in its invasive stage. The role of total pancreatectomy was reappraised in the treatment of IPMT., Methodology: A total of five Japanese patients with IPMT underwent total pancreatectomy and their clinical follow-up data were reviewed., Results: Total pancreatectomy was performed due to massive involvement of the entire pancreas in two patients, positive surgical margins on frozen section in one, benign IPMT with concomitant pancreatic cancers in one and recurrent IPMT in the remnant pancreas after distal pancreatectomy for IPMT in the other. Three of them underwent total pancreatectomy of the Whipple type, another underwent total gastrectomy and the other underwent the pylorus-preserving method. Surgical margins were negative by histology and no lymph node metastases were evident. Two patients had severe infection including liver abscess in one and pneumonia in the other. The former died on postoperative day 82 and the latter was controlled by medical treatment and discharged on postoperative day 73. The other three patients had an uneventful postoperative course and were discharged from 29 to 62 days after the operation. Long-term follow-up of the four patients revealed that three patients had hypoglycemic attacks, two diabetic retinopathy and two fatty liver. The four patients were doing well from 683 to 4,140 days after the operation without signs of recurrence., Conclusions: Total pancreatectomy would be indicated as a treatment of benign or malignant IPMT with extensive involvement when patients' condition permits and gives a chance of cure, although careful long-term medical care and follow-up are essential.
- Published
- 2005
50. ERT following IORT improves survival of patients with resectable pancreatic cancer.
- Author
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Yamaguchi K, Nakamura K, Kobayashi K, Nakano K, Konomi H, Mizumoto K, and Tanaka M
- Subjects
- Aged, Female, Humans, Male, Middle Aged, Pancreatic Neoplasms mortality, Radiotherapy, Adjuvant, Retrospective Studies, Splenectomy, Survival Rate, Treatment Outcome, Pancreatectomy, Pancreatic Neoplasms radiotherapy, Pancreatic Neoplasms surgery, Pancreaticoduodenectomy
- Abstract
Background/aims: The clinical course of patients with pancreatic carcinoma remains dismal despite the recent advances of diagnostic and therapeutic procedures. One of the main causes is residual carcinoma cells, especially at the retroperitoneal aspect after pancreatectomy. Radiation therapy (RT) [intraoperative radiation therapy (IORT) and external radiation therapy (ERT)] is a therapeutic strategy to conquer the remaining cancer cells., Methodology: Effects of RT were retrospectively examined in 81 patients with pancreatectomy for pancreatic cancer together with early and late complications., Results: Preoperative clinical data were not different between the RT(+) and RT(-) groups excluding peripheral lymphocyte counts. Postoperative early complications equally occurred including pancreatic fistula. Regarding late complications (>2 months after operation), stomal ulcer, vertebral fracture, pseudoaneurysm, intraabdominal hemorrhage, and liver abscess were present only in patients with RT. Glucose intolerance tended to be more frequent in the RT (+) group, i.e. 12 (63%) of the 19 with RT examined and 14 (42%) of the 33 without RT examined. Follow-up imaging showed recurrence in 27 (71%) of the 38 patients without RT and 13 (52%) of the 25 patients with RT. The sites of the recurrence were not different by the presence or absence of RT. One-year, 3-year and 5-year cumulative survival rates were similar between the RT (-) group (100%, 39%, 21%, respectively) and IORT (+) alone group (100%, 29% and 17%, respectively). The rates in the IORT (+) and ERT (+) group were 100%, 54% and 28%, respectively, which tended to be better than those in the other two groups, but the differences were not statistically significant., Conclusions: These findings suggest that only the combination of IORT and ERT somewhat improves the short-term clinical course of patients with resectable pancreatic cancer, although there are some RT-related late complications. It is recommended that ERT be added to IORT after pancreatectomy for pancreatic cancer to improve the clinical course, once IORT has been performed.
- Published
- 2005
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