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Two new triterpene saponins, named hippophosides E and F ( 1 and 2, resp.), together with two known compounds, were isolated from the seed residue of Hippophae rhamnoides L. Their structures were elucidated on the basis of chemical and spectral analysis, including 1D- and 2D-NMR and HR-MS experiments, and by comparison with literature data. [ABSTRACT FROM AUTHOR]

Four new triterpenoid saponins (1–4) were isolated from the seed residue ofHippophae rhamnoidessubsp.sinensis, named 3-O-[β-d-glucopyranosyl(1 → 2)-β-d-glucopyranosyl-(1 → 3)]-[α-l-rhamnopyranosyl-(1 → 2)]-α-l-arabinopyranosyl-13-ene-19-one-28-oic acid 28-O-β-d-glucopyranosyl ester (1), 3-O-[β-d-glucopyranosyl(1 → 2)-β-d-glucopyranosyl-(1 → 3)]-[α-l-rhamnopyranosyl-(1 → 2)]-α-l-arabinopyranosyl-13-ene-19-one-30-hydroxyolean-28-oic acid 28-O-β-d-glucopyranosyl ester (2), 3-O-[β-d-glucopyranosyl(1 → 2)-β-d-glucopyranosyl-(1 → 3)]-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranosyl-13-ene-19-one-28-oic acid 28-O-β-d-glucopyranosyl ester (3), and 3-O-[β-d-glucopyranosyl(1 → 2)-β-d-glucopyranosyl-(1 → 3)]-[α-l-rhamnopyranosyl-(1 → 2)]-β-d-glucopyranosyl-13-ene-19-one-30-hydroxyolean-28-oic acid 28-O-β-d-glucopyranosyl ester (4), and their structures were elucidated on the basis of spectroscopic and chemical methods. [ABSTRACT FROM PUBLISHER]

A series of flavone glycosides were isolated from Fructus Kochiae for the first time, including two new flavone glycosides. The structures were established by interpretation of their spectroscopic data. Two new flavone glycosides are quercetin 3-O-β-d-apiofuranosyl-(1 → 2)-β-d-galactopyranosyl-7-O-β-d-glucopyranoside (1) and quercetin 3-O-α-l-rhamnopyranosyl-(1 → 6)-β-d-galactopyranosyl-7-O-β-d-sophoroside (2). The others are quercetin 7-O-β-d-glucopyranoside (3), quercetin 3-O-β-d-apiofuranosyl-(1 → 2)-β-d-galactopyranoside (4), quercetin 3-O-β-d-galactopyranosyl-7-O-β-d-glucopyranoside (5), and quercetin 7-O-β-d-sophoroside (6). [ABSTRACT FROM PUBLISHER]

Three new flavonol glycosides (1–3), hippophins K–M, were isolated from the seed residue of Hippophae rhamnoides subsp. sinensis. The chemical structures of these three new compounds were identified by 1D, 2D NMR, mass spectroscopy and high resolution electrospray ionization mass spectrometry spectroscopic data and by comparison with the literature data. This report is a continuous research work on the systematic chemical investigation of plants of the genus Hippophae in our laboratory. [ABSTRACT FROM AUTHOR]

Four new flavonol glycosides (1–4), hippophins C–F, together with one known flavonoid (5), were isolated from the seed residue of Hippophae rhamnoides subsp. sinensis. The chemical structures of these compounds were characterized by 1D and 2D NMR, and HR-ESI-MS data. This report is a continuous research work on the systematic chemical investigation of plants of the genus Hippophae in our laboratory. [ABSTRACT FROM AUTHOR]

Three new flavonoid glycosides, kaempferol-3-O-β-d-apiofuranosyl(1 → 2)-β-d-glucopyranosyl-7-O-α-l-rhamnopyranoside (1), kaempferol-4′-O-β-d-apiofuranosyl-3-O-β-d-glucopyranosyl-7-O-α-l-rhamnopyranoside (2), and 5,6,7,4′-tetrahydroxy-flavone-6-O-β-d-arabinopyranosyl-7-O-α-l-rhamnopyranoside (3), were isolated from the aerial parts of Urena lobata L., along with 10 known compounds (4–13). Their structures were determined based on spectroscopic methods including 1D and 2D NMR spectroscopy as well as HR-ESI-MS. [ABSTRACT FROM PUBLISHER]

Two new dammarane monodesmosides centellosides A (1) and B (2), and two new natural products ginsenosides Mc (10) and Y (11), together with 11 known compounds (3-9 and 12-15) reported for the first time from this genus, were isolated from the whole plants of Centella asiatica. All structures were elucidated by spectroscopic techniques and chemical methods, and compared with literature values. All the isolated compounds were evaluated in vitro for cytotoxicity. [ABSTRACT FROM AUTHOR]

Two new compounds 1 and 2 have been isolated from the aerial parts of Urena lobata L. The structures of the two new compounds were established as ceplignan-4-O-β-d-glucoside (1) and 2,5-dihydroxy benzoic acid-7-(2,6-dimethyl-6-hydroxy-2,7-octadienoic acid) anhydride-5-O-β-d-apiofuranosyl(1 → 2)-β-d-glucoside (urenoside A) (2), on the basis of chemical and spectral evidence, including 1D and 2D NMR spectroscopic data as well as mass spectrometry (HR-ESI-MS). [ABSTRACT FROM AUTHOR]

Bioassay-guided methods were used to test the antitumor activity of methanol extract of the whole plant of Bacopa monniera (L.) Wettst. and four different fractions (petroleum ether, CHCl3, EtOAc, and n-BuOH fractions) of the methanol extract. Among the five crude samples, n-BuOH fraction was noted to have the highest antitumor activity. The dammarane triterpene saponins isolated from n-BuOH fraction, bacopaside É (1) and bacopaside VII (3), had potential antitumor effect. 1 and 3 showed cytotoxicity of all the tested human tumor cell lines MDA-MB-231, SHG-44, HCT-8, A-549 and PC-3M in MTT assay in vitro, and showed 90.52 % and 84.13 % inhibition in mouse implanted with sarcoma S180 in vivo at the concentration of 50 μmol/kg, respectively. The remaining two compounds, bacopaside II (2) and bacopasaponin C (4) were found to be much less potent compared with 1 and 3. 1 and 3 significantly inhibited human breast cancer cell line MDA-MB-231 adhesion, migration and Matrigel invasion in vitro at the concentration of 50 μmol/L. Since no antitumor activities about the monomers from Bacopa monniera (L.) Wettst. have been reported, these results indicate that the mechanism of action of 1 and 3 needs further study. Copyright © 2009 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Objective: To analyze the chemical constituents of the essential oil from Callicarpa kwangtungensis and investigate their antimicrobial activity in vitro., Methods: The essential oil of Callicarpa kwangtungensis were extracted by steam distillaton. The chemical constituents were separated and analyzed by GC-MS. Their relative percentages were calculated with peak area normalization method., Results: 38 compounds were identified, accounting for 76.01% of the peak area of the total ion-current chromatogram. The essential oil had different antimicrobial activities., Conclusion: The main constituent of the essential oil is terpenoids (59.25%), and showing different activities against Staphylococcus aureus, Escherichia coli and Candida albicans.

Three new alkaloids (1-3), together with ten known alkaloids, were isolated from the ethanolic extract of the whole plants of Lycopodium japonicum THUNB. Their structures were elucidated on the basis of spectroscopic analysis, including MS and NMR methods. All alkaloids isolated were assayed for cytotoxic activity against four human cancer cell lines and acetylcholinesterase (AChE) inhibitory activity. No alkaloid showed either cytotoxic activity against four human cancer cell lines or AChE inhibitory activity.

A new C(14) pterosin sesquiterpenoid, named (2R)-pterosin P (1), and a new natural product, named dehydropterosin B (3), were isolated from the aerial parts of Pteris multifida Poir., along with nine known compounds (2, 4-11). By chiral HPLC, compounds 1 and 2 were isolated as a pair of enantiomeric pterosin sesquiterpenoids. The planar structure of 1 was elucidated on the basis of NMR spectroscopy analysis, and the absolute configuration was established by the CD spectrum. In addition, the absolute structure of 1 was further confirmed by single-crystal X-ray diffraction (CuK α). Compounds 3, 5, and 6 showed potent cytotoxicity against PANC-1 (human pancreatic cancer) and NCI-H446 (human small-cell lung cancer) cell lines, with IC(50) values in the range of 4.27-14.63 µM., (© Georg Thieme Verlag KG Stuttgart · New York.)

Three new saponins, bacopasides IX-XI (1- 3), together with their known analogues bacopaside I (4), bacopaside II (5), bacopasaponsin C (6), and bacopasaponsin D (7), were isolated from the whole plant of Bacopa monniera. Compounds 3, 4, and 6 showed nootropic activity when tested in the Morris water maze test and step-down test of scopolamine-induced memory impairment in mice., (Copyright Georg Thieme Verlag KG Stuttgart. New York.)

Objective: To compare the influence of traditional Chinese compound recipes (TCCRs) with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice., Methods: H(22) cancer-bearing mice were chosen to observe the effects of TCCRs with different efficacy on tumor growth inhibition and detect the proliferation function of T lymphocytes, the activity of natural killer (NK) cells, the changes of T lymphocytes and the content of interferon-gamma (IFN-gamma)and interleukin-4 (IL-4)., Results: Tumor weight of H(22) cancer-bearing mice in Yidu Gongdu Recipe (YDGDR, a compound traditional Chinese herbal medicine using poison as an antidote for poison)-treated group was obviously lighter than that in the other TCCR-treated groups and the tumor inhibition rate in YDGDR-treated group was 65.76% (P<0.01). The tumor inhibition rates in other TCCR-treated groups were ranged from 10.1% to 17.1% . Body weight of mice in YDGDR-treated group was obviously decreased and depilation was observed at the same time. Pelage of mice in Fuzheng Peiben Recipe (FZPBR, a compound traditional Chinese herbal medicine for supporting the healthy energy)-treated group grew well, and behavior of the mice was active. Stimulation index (SI) of T lymphocyte transformation in YDGDR-treated group was obviously increased (SI=4.34, P<0.01), which showed the proliferation function of T lymphocyte was very strong. The SI of T lymphocyte transformation in the other groups was less than three, which showed the proliferation function of T lymphocytes was not significant. Compared with normal saline (NS)-treated group, percentages of NK cells in Qinre Jiedu Recipe (QRJDR, a compound traditional Chinese herbal medicine for clearing away heat and toxic substances)-treated, Huxue Huayu Recipe (HXHYR, a compound traditional Chinese herbal medicine for activating blood circulation to dissipate blood stasis)-treated and YDGDR-treated groups were obviously increased and 5.05, 4.07 and 5.17 times more than the NS-treated group, respectively (P<0.01). The activity of NK cells wasn't increased in the FZPBR-treated and HXHYR-treated groups. The production of IFN-gamma induced by T cells in YDGDR-treated group was obviously raised (P<0.05), and the production of IL-4 induced by T cells in QRJDR-treated, HXHYR-treated, Huatan Sanjie Recipe (a compound traditional Chinese herbal medicine for eliminating phlegm and resolving masses)-treated and YDGDR-treated groups was also raised obviously (P<0.01)., Conclusion: YDGDR has a good effect of inhibiting tumor growth and can reinforce cellular and humoral immune function in tumor-bearing mice. FZPBR can strengthen the body.

Objective: To investigate the mechanisms of tumor inhibiting and immunoloregulation of Mylabris Mixture on H22 cancer-bearing mice., Methods: H22 cancer-bearing mice were chosen to observe the effects of tumor inhibiting and detect the proliferation function of T lymphocytes, the toxicity function of NK cells, the changes of T lymphocytes and the contents of interferon-gamma and interleukin-4., Results: Mylabris Mixture could obviously inhibit the growth of H22 cancer in mice, and the tumor inhibition rat was 65.76%. The stimulation index of T lymphocyte transformation and percentage of NK cells in Mylabris Mixture-treated group were obviously higher than those in the normal control group. The subpopulation proportion of T lymphocytes in Mylabris Mixture-treated group was changed more than the normal control group. The production of interferon-gamma and interleukin-4 by T lymphocytes obviously increased in Mylabris Mixture-treated group (P<0.05, P<0.001)., Conclusion: Mylabris Mixture has the effect of inhibiting the growth of tumor constitution, and regulating immunological function on mice with tumor. Its mechanisms include the reinforcement of T lymphocyte immune function, NK cell killing function and humoral immune function.

Aim: To study the bioactive components from Helicia nilagirica., Methods: Compounds were separated with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence., Results: Two compounds were isolated from the leaves of Helicia nilagirica. Compound 1 was elucidated as 1-O-3-D-glucopyranosyl-(2S,3S,4R,8Z)-2-[(2'R)-2'-hyd roxylignocenoyl-amino]-8-octadecene-1, 3, 4-triol. Compound 2 was an analogue of 1., Conclusion: The two compounds are new cerebrosides.

Aim: To study the bioactive components from Schisandra propinqua (Wall.) Baill var. intermidia A. C. Smith., Methods: Compounds were separated with a combination of multichromatography. Their chemical structures were determined on the basis of spectral analysis and chemical evidence., Results: Seven compounds were isolated from the leaves of Helicia nilagirica. The structures were elucidated as 6'-O-alpha-L-arabinofuranosylthalictoside (1), 6'-O-beta-D-apiofuranosylthalictoside (2), thalictoside (3), icariside D2 (4), prinsepiol (5), (+)-1-hydroxypinoresinol (6) and (+)-medioresinol (7)., Conclusion: Two compounds are new nitro phenolic glycosides.