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20 results on '"Kong, S.C."'

2. Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction

Author

Fite, A., Macfarlane, G.T., Cummings, J.H., Hopkins, M.J., Kong, S.C., Furrie, E., and Macfarlane, S.

Subjects
Sulfate-reducing bacteria -- Care and treatment -- Research -- Health aspects, Epithelial cells -- Health aspects -- Research, Desulfuration -- Research -- Health aspects, Health, Care and treatment, Research, Health aspects
Abstract

Gut 2004;53:523-529. doi: 10.1136/gut.2003.031245 Background: Desulfovibrios produce sulphide, which is toxic to colonic epithelial cells. These bacteria have previously been linked to ulcerative colitis. Traditional methods of culturing these organisms [...]

Published
2004

3. Luminescent Dual Sensors Reveal Extracellular pH-Gradients and Hypoxia on Chronic Wounds That Disrupt Epidermal Repair

Author

Schreml, S., Meier, R.J., Kirschbaum, M., Kong, S.C., Gehmert, S., Felthaus, O., Küchler, S., Sharpe, J.R., Wöltje, K., Weiß, K.T., Albert, M., Seidl, U., Schröder, J., Morsczeck, C., Prantl, L., Duschl, C., Pedersen, S.F., Gosau, M., Berneburg, M., Wolfbeis, O.S., Landthaler, M., Babilas, P., and Publica

Subjects
Keratinocytes, Male, Sodium-Hydrogen Exchangers, cell migration, proton transporters, 610 Medizin, wound healing, Biosensing Techniques, Cell Line, Varicose Ulcer, gradient sensing, fluorescence imaging, wound healing, cell migration, gradient sensing, proton transporters, Re-Epithelialization, fluorescence imaging, Cell Movement, Humans, Cation Transport Proteins, Aged, Cell Proliferation, Fluorescent Dyes, ddc:610, Sodium-Hydrogen Exchanger 1, Optical Imaging, Hydrogen-Ion Concentration, Middle Aged, Cell Hypoxia, Microscopy, Fluorescence, Female, Research Paper
Abstract

Wound repair is a quiescent mechanism to restore barriers in multicellular organisms upon injury. In chronic wounds, however, this program prematurely stalls. It is known that patterns of extracellular signals within the wound fluid are crucial to healing. Extracellular pH (pHe) is precisely regulated and potentially important in signaling within wounds due to its diverse cellular effects. Additionally, sufficient oxygenation is a prerequisite for cell proliferation and protein synthesis during tissue repair. It was, however, impossible to study these parameters in vivo due to the lack of imaging tools. Here, we present luminescent biocompatible sensor foils for dual imaging of pHe and oxygenation in vivo. To visualize pHe and oxygen, we used time-domain dual lifetime referencing (tdDLR) and luminescence lifetime imaging (LLI), respectively. With these dual sensors, we discovered centripetally increasing pHe-gradients on human chronic wound surfaces. In a therapeutic approach, we identify pHe-gradients as pivotal governors of cell proliferation and migration, and show that these pHe-gradients disrupt epidermal barrier repair, thus wound closure. Parallel oxygen imaging also revealed marked hypoxia, albeit with no correlating oxygen partial pressure (pO2)-gradient. This highlights the distinct role of pHe-gradients in perturbed healing. We also found that pHe-gradients on chronic wounds of humans are predominantly generated via centrifugally increasing pHe-regulatory Na+/H+-exchanger-1 (NHE1)-expression. We show that the modification of pHe on chronic wound surfaces poses a promising strategy to improve healing. The study has broad implications for cell science where spatial pHe-variations play key roles, e.g. in tumor growth. Furthermore, the novel dual sensors presented herein can be used to visualize pHe and oxygenation in various biomedical fields.

Published
2014

6. Gender and computer-supported collaborative learning

Author

Ding, N., Kong, S.C., Ogata, H., Arnseth, H.C., Chan, C.K.K., Hirashima, T., Klett, F., Yang, S.J.H., Research and Evaluation of Educational Effectiveness, and Nieuwenhuis Institute (Pedagogical and Educational Sciences)

Published
2009

7. Synbiotic consumption increases expression of mRNA for hydrogen sulphide detoxification enzymes

Author

Kong, S.C., Furrie, E., Kennedy, A., Macfarlane, G.T., and Cummings, J.H.

Subjects
Ulcerative colitis -- Care and treatment -- Research, Hydrogen sulfide -- Health aspects -- Research, Detoxification therapy (Alternative medicine) -- Health aspects -- Research, Health, Care and treatment, Research, Health aspects
Abstract

Introduction: Bacterial metabolism of oxidised sulphur containing compounds has been linked to ulcerative colitis (UC). Probiotics have been shown to be useful in the treatment of UC, but the therapeutic [...]

Published
2004

8. Proceedings of the 17th International Conference on Computers in Education (ICCE) 2009

Author

Milrad, Marcelo, Kong, S.C., Ogata, H., Arnseth, H.C., Chan, C.K.K., Hirashima, T., Klett, F., Lee, J.H.M., Liu, C.C., Looi, C.K., , Mitrovic, A., Nakabayashi, K., Wong, S.L. & Yang, S.J.H., Siu Cheung, Milrad, Marcelo, and Kong, S.C., Ogata, H., Arnseth, H.C., Chan, C.K.K., Hirashima, T., Klett, F., Lee, J.H.M., Liu, C.C., Looi, C.K., , Mitrovic, A., Nakabayashi, K., Wong, S.L. & Yang, S.J.H., Siu Cheung

Published
2009

10. Comparison of hydrogen sulphide detoxification enzyme mrna expression in normal and ulcerative colitis rectal mucosae

Author

Kong, S.C., Furrie, E., Madden, J., Kennedy, A., Magee, E., Macfarlane, G.T., and Cummings, J.H.

Subjects
Ulcerative colitis -- Care and treatment -- Research, Sulfites -- Health aspects -- Research, Hydrogen sulfide -- Research -- Health aspects, Health, Care and treatment, Research, Health aspects
Abstract

Introduction: Hydrogen sulphide ([H.sub.2]S) produced in the colon is toxic to the colonic mucosa and may be involved in the pathogenesis of ulcerative colitis (UC). The exact pathway of [H.sub.2]S [...]

Published
2004

11. Dietary sulphite and disease activity in ulcerative colitis patients

Author

Magee, E.A., Tasker, S.M., Edmond, L.M., Kong, S.C., and Cummings, J.H.

Subjects
Ulcerative colitis -- Causes of -- Care and treatment -- Research, Sulfites -- Health aspects -- Research, Health, Care and treatment, Research, Health aspects, Causes of
Abstract

Background: The cause of ulcerative colitis (UC) is unknown, although the relapsing nature and geographical incidence of the disease implicate environmental factors. Sulphiting agents are widely used food additives. Due [...]

Published
2004

20. PATIENTS WITH INFLAMMATORY BOWEL DISEASE TAKE AS MUCH COMPLEMENTARY/ALTERNATIVE MEDICINE AS PATIENTS WITH FUNCTIONAL BOWEL DISEASE.

Author

Kong, S.C., Pocock, C.Y., Hurlstone, D.P., Bramble, M.G., McAlindon, M.E., and Sanders, D.S.

Subjects
*INFLAMMATORY bowel diseases, *ALTERNATIVE medicine, *INTESTINAL diseases
Abstract

Introduction: In recent years, the public awareness and usage of complementary/alternative medicine (CAM) has increased. The use of CAM (for example, aloe vera) has previously been described in adult patients with functional bowel disease. Aim: To assess the incidence of CAM usage by gastroenterology patients at a single university centre. Method: Patients attending luminal gastroenterology clinics (from June 2002) completed a 30-point, structured questionnaire assessing their use of CAM. A gastroenterologist verified the patient diagnosis. Results: There were 790 patients recruited. The 3 main diagnostic categories were inflammatory bowel disease (IBD), irritable bowel syndrome (lBS), and other gastrointestinal diseases (GID: for example colonic polyps). Demographics and CAM usage in each group are shown in the table below. Chi-squared analysis demonstrated significant differences between the lBS versus other Gl group (p<0.0001) and IBD versus other Gl group (p<0.0001). Multivariate logistical analysis of the whole cohort showed significant demographic differences for sex: female versus male (p<0.05). 64% of all CAM users were women. For every 1-year increase in age there was a 1% reduction in the use of CAM (p<0.03). 87% of all CAM users (253/291) felt they derived some symptomatic benefit. Conclusion: The percentage of CAM users amongst patients with IBD is similar to those with a functional diagnosis. Patients with IBD are using CAM in addition to conventional treatment. [ABSTRACT FROM AUTHOR]

Published
2003

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