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308 results on '"Komorowski L"'

1. Improving the sensitivity of myelin oligodendrocyte glycoprotein-antibody testing: exclusive or predominant MOG-IgG3 seropositivity—a potential diagnostic pitfall in patients with MOG-EM/MOGAD

Author

Jarius, S., Ringelstein, M., Schanda, K., Ruprecht, K., Korporal-Kuhnke, M., Viehöver, A., Hümmert, M. W., Schindler, P., Endmayr, V., Gastaldi, M., Trebst, C., Franciotta, D., Aktas, O., Höftberger, R., Haas, J., Komorowski, L., Paul, F., Reindl, M., and Wildemann, B.

Published
2024
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5. 067 Laminin beta4: A protein of the cutaneous basal membrane zone and autoantigen of anti-p200 pemphigoid

Author

Goletz, S., primary, Pigors, M., additional, Lari, T. Rastegar, additional, Hammers, C.M., additional, Wang, Y., additional, Emtenani, S., additional, Aumailley, M., additional, Holtsche, M.M., additional, Stang, F., additional, Weyers, I., additional, König, I., additional, Has, C., additional, Radzimski, C., additional, Komorowski, L., additional, Zillikens, D., additional, and Schmidt, E., additional

Published
2023
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10. The effect of size and distribution of inert pigment on the performance of organic coatings

Author

Królikowska A., Komorowski L., Bonora P.L., Królikowska A., Komorowski L., and Bonora P.L.

Abstract

The paper aims to show how the performance of paint coatings is affected by their composition. Samples of two paint coatings, which had delaminated in service, were compared with the same type of paint applied to samples for the purposes of certification. The particle size distribution of the filler materials (inert pigments) affected the flow of resin and wetting of the particles. In both failed samples, large particles sizes had agglomerated creating voids and crevices, resulting in poor wetting by the resin. This poor wetting and inherent lack of bonding had resulted in their premature failure. Both equivalent laboratory samples had a much better particle size distribution, with a lower maximum particle size, resulting in inert pigment being well distributed through the paint layer. The paper concludes that tighter control over the sizes of additives in paint such as inert pigments within Standards is needed to ensure better performance., The paper aims to show how the performance of paint coatings is affected by their composition. Samples of two paint coatings, which had delaminated in service, were compared with the same type of paint applied to samples for the purposes of certification. The particle size distribution of the filler materials (inert pigments) affected the flow of resin and wetting of the particles. In both failed samples, large particles sizes had agglomerated creating voids and crevices, resulting in poor wetting by the resin. This poor wetting and inherent lack of bonding had resulted in their premature failure. Both equivalent laboratory samples had a much better particle size distribution, with a lower maximum particle size, resulting in inert pigment being well distributed through the paint layer. The paper concludes that tighter control over the sizes of additives in paint such as inert pigments within Standards is needed to ensure better performance.

Published
2021

24. PF161 THIOREDOXIN SYSTEM INHIBITORS ARE EFFECTIVE AGAINST B CELL ACUTE LYMPHOBLASTIC LEUKEMIA IN VITRO AND IN VIVO

Author

Fidyt, K., primary, Pastorczak, A., additional, Goral, A., additional, Muchowicz, A., additional, Komorowski, L., additional, Cyran, J., additional, Pal, D., additional, Blair, H., additional, Burdzinska, A., additional, Pedzisz, P., additional, Patkowska, E., additional, Lech-Maranda, E., additional, Mlynarski, W., additional, Golab, J., additional, and Firczuk, M., additional

Published
2019
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26. The effect of size and distribution of inert pigment on the performance of organic coatings.

Author

Królikowska, A., Komorowski, L., and Bonora, P. L.

Subjects
*ORGANIC coatings, *PARTICLE size distribution, *ORGANIC dyes, *PIGMENTS, *FILLER materials, *EMULSION paint
Abstract

The paper aims to show how the performance of paint coatings is affected by their composition. Samples of two paint coatings, which had delaminated in service, were compared with the same type of paint applied to samples for the purposes of certification. The particle size distribution of the filler materials (inert pigments) affected the flow of resin and wetting of the particles. In both failed samples, large particles sizes had agglomerated creating voids and crevices, resulting in poor wetting by the resin. This poor wetting and inherent lack of bonding had resulted in their premature failure. Both equivalent laboratory samples had a much better particle size distribution, with a lower maximum particle size, resulting in inert pigment being well distributed through the paint layer. The paper concludes that tighter control over the sizes of additives in paint such as inert pigments within Standards is needed to ensure better performance. [ABSTRACT FROM AUTHOR]

Published
2021
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27. Intracellular and non-neuronal targets of voltage-gated potassium channel complex antibodies

Author

Lang, B, Makuch, M, Moloney, T, Dettmann, I, Mindorf, S, Probst, C, Stoecker, W, Buckley, C, Newton, C, Leite, I, Maddison, P, Komorowski, L, Adcock, J, Vincent, A, Waters, P, and Irani, S

Subjects
Neuro-Inflammation, Elapid Venoms, Neurons, Brain Diseases, Intracellular Signaling Peptides and Proteins, Intracellular Space, Brain, Membrane Proteins, Proteins, Nerve Tissue Proteins, Neuromuscular Diseases, Hippocampus, Cohort Studies, Iodine Radioisotopes, Epitopes, Autoimmune Diseases of the Nervous System, Cytosol, HEK293 Cells, Potassium Channels, Voltage-Gated, Shaker Superfamily of Potassium Channels, Humans, Autoantibodies
Abstract

Objectives Autoantibodies against the extracellular domains of the voltage-gated potassium channel (VGKC) complex proteins, leucine-rich glioma-inactivated 1 (LGI1) and contactin-associated protein-2 (CASPR2), are found in patients with limbic encephalitis, faciobrachial dystonic seizures, Morvan’s syndrome and neuromyotonia. However, in routine testing, VGKC-complex-antibodies without LGI1- or CASPR2-reactivities (“double-negative”) are commoner than LGI1- or CASPR2-specificities. Therefore, the target(s) and clinical associations of double-negative antibodies need to be determined. Methods Sera (n=1131) from several clinically-defined cohorts were tested for IgG-radioimmunoprecipitation of 125I-DTX-labelled VGKC-complexes from mammalian brain extracts. Positive samples were systematically tested for live hippocampal neuron reactivity, IgG-precipitation of 125I-DTX and 125I-DTX-labelled Kv1-subunits, and by cell-based assays which expressed Kv1-subunits, LGI1 and CASPR2. Results VGKC-complex-antibodies were found in 162 of 1131 (14%) sera. Ninety of these (56%) had antibodies targeting the extracellular domains of LGI1 or CASPR2. Of the remaining 72 double-negative sera, ten (14%) immunoprecipitated 125I-DTX itself, and 27 (38%) bound to solubilized co-expressed Kv1.1/1.2/1.6 subunits and/or Kv1.2 subunits alone, at levels proportionate to VGKC-complex-antibody levels (r=0.57, p=0.0017). The sera with LGI1- and CASPR2-antibodies immunoprecipitated neither preparation. None of the 27 Kv1-precipitating samples bound live hippocampal neurons or Kv1-extracellular domains, but 16 (59%) bound to permeabilised Kv1-expressing HEK cells. These intracellular Kv1-antibodies mainly associated with non-immune disease aetiologies, poor longitudinal clinical-serological correlations, and a limited immunotherapy-response. Conclusions Double-negative VGKC-complex-antibodies are often directed against cytosolic epitopes of Kv1-subunits, and occasionally against non-mammalian DTX. These antibodies should no longer be classified as neuronal-surface antibodies. They consequently lack pathogenic potential, and do not in themselves support use of immunotherapies.

Published
2017

28. A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid

Author

Goletz, S., primary, Probst, C., additional, Komorowski, L., additional, Schlumberger, W., additional, Fechner, K., additional, van Beek, N., additional, Holtsche, M.M., additional, Recke, A., additional, Yancey, K.B., additional, Hashimoto, T., additional, Antonicelli, F., additional, Di Zenzo, G., additional, Zillikens, D., additional, Stöcker, W., additional, and Schmidt, E., additional

Published
2018
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29. 089 Sensitive and specific assay for the serological diagnosis of anti-laminin 332 mucous membrane pemphigoid

Author

Goletz, S., primary, Probst, C., additional, Komorowski, L., additional, Schlumberger, W., additional, van Beek, N., additional, Holtsche, M.M., additional, Recke, A., additional, Yancey, K.B., additional, Hashimoto, T., additional, Antonicelli, F., additional, Di Zenzo, G., additional, Zillikens, D., additional, Stöcker, W., additional, and Schmidt, E., additional

Published
2018
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30. Anti-Hu antibodies activate enteric and sensory neurons

Author

Li, Q., Michel, K., Annahazi, A., Demir, I.E., Ceyhan, G.O., Zeller, F., Komorowski, L., Stöcker, W., Beyak, M.J., Grundy, D., Farrugia, G., De Giorgio, R., and Schemann, M.

Subjects
Male, Multidisciplinary, Sensory Receptor Cells, Antibodies, Neoplasm, Guinea Pigs, ELAV-Like Protein 4, Article, Antibodies, Enteric Nervous System, NO, Animals, Female, Humans, nervous system, Neoplasm
Abstract

IgG of type 1 anti-neuronal nuclear antibody (ANNA-1, anti-Hu) specificity is a serological marker of paraneoplastic neurological autoimmunity (including enteric/autonomic) usually related to small-cell lung carcinoma. We show here that IgG isolated from such sera and also affinity-purified anti-HuD label enteric neurons and cause an immediate spike discharge in enteric and visceral sensory neurons. Both labelling and activation of enteric neurons was prevented by preincubation with the HuD antigen. Activation of enteric neurons was inhibited by the nicotinic receptor antagonists hexamethonium and dihydro-β-erythroidine and reduced by the P2X antagonist pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid (PPADS) but not by the 5-HT3 antagonist tropisetron or the N-type Ca-channel blocker ω-Conotoxin GVIA. Ca(++) imaging experiments confirmed activation of enteric neurons but not enteric glia. These findings demonstrate a direct excitatory action of ANNA-1, in particular anti-HuD, on visceral sensory and enteric neurons, which involves nicotinic and P2X receptors. The results provide evidence for a novel link between nerve activation and symptom generation in patients with antibody-mediated gut dysfunction.

Published
2016

31. A case of late-onset, thymoma-associated myasthenia gravis with ryanodine receptor and titin antibodies and concomitant granulomatous myositis

Author

Stefanou, M. I., Komorowski, L., Kade, S., Bornemann, A., Ziemann, U., and Synofzik, M.

Subjects
immunology [Ryanodine Receptor Calcium Release Channel], Thymoma, Clinical Neurology, complications [Myasthenia Gravis], Case Report, immunology [Connectin], immunology [Thymoma], immune system diseases, Myasthenia Gravis, immunology [Autoantibodies], Humans, Connectin, ddc:610, complications [Myositis], Aged, immunology [Myositis], Autoantibodies, Myositis, complications [Thymoma], Ryanodine receptor antibodies, Ryanodine Receptor Calcium Release Channel, immunology [Myasthenia Gravis], nervous system diseases, Titin antibodies, Granulomatous myositis, Female
Abstract

Background Myasthenia gravis is an autoimmune neuromuscular disorder, which has only rarely been reported to co-manifest with myositis. The diagnosis of concomitant myositis in patients with myasthenia gravis is clinically challenging, and requires targeted investigations for the differential diagnosis, including EMG, autoantibody assays, muscle biopsy and, importantly, imaging of the mediastinum for thymoma screening. Case presentation This report presents a case-vignette of a 72-year-old woman with progressive proximal muscle weakness and myalgias, diagnosed with thymoma-associated myasthenia and bioptically verified granulomatous myositis, with positive autoantibody status for ryanodine receptor and titin antibodies. Conclusions The diagnosis of concurrent myositis and myasthenia gravis, especially in the presence of ryanodine receptor and titin antibodies, should lead neurologists to adopt different treatment strategies compared to those applied in myasthenia or myositis alone. Moreover, further evidence is warranted that titin and, particularly, ryanodine receptor antibodies may co-occur or be pathophysiologically involved in myasthenia-myositis cases.

Published
2016

36. Identification of the flotillin-1/2 heterocomplex as a target of autoantibodies in bona fide multiple sclerosis

Author

Hahn, S., primary, Trendelenburg, G., additional, Scharf, M., additional, Denno, Y., additional, Brakopp, S., additional, Teegen, B., additional, Probst, C., additional, Wandinger, K. P., additional, Buttmann, M., additional, Haarmann, A., additional, Szabados, F., additional, vom Dahl, M., additional, Kümpfel, T., additional, Eichhorn, P., additional, Gold, H., additional, Paul, F., additional, Jarius, S., additional, Melzer, N., additional, Stöcker, W., additional, and Komorowski, L., additional

Published
2017
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39. Normal human skin is superior to monkey oesophagus substrate for detection of circulating BP180‐NC16A‐specific IgG antibodies in bullous pemphigoid.

Author

Emtenani, S., Yuan, H., Lin, C., Pan, M., Hundt, J.E., Schmidt, E., Komorowski, L., Stanley, J.R., and Hammers, C.M.

Subjects
BULLOUS pemphigoid, ESOPHAGUS, ENZYME-linked immunosorbent assay, MEMBRANE proteins, IMMUNOGLOBULINS, MONOCLONAL antibodies
Abstract

Summary: Background: Bullous pemphigoid (BP) is the most common autoimmune subepidermal blistering skin disease. Two antigens have been identified as targets of circulating autoantibodies (autoAbs) – BP180 and BP230 – with BP180 being a critical transmembrane adhesion protein of basal keratinocytes of the epidermis. The noncollagenous domain 16A (NC16A) of BP180 is the immunodominant epitope in patients with BP, and anti‐BP180‐NC16A IgG antibodies (Abs) correlate to disease activity. Routine serological testing and follow‐up of BP relies on indirect immunofluorescence (IIF) of serum Abs, commonly performed on monkey oesophagus (ME), and/or enzyme‐linked immunosorbent assay (ELISA) testing on recombinantly produced fragments of BP180 and BP230 (BP180‐NC16A, BP230‐C/N). Objectives: To determine if NC16A epitopes are well represented on ME substrate. Methods: Sera from different BP cohorts were tested by IIF on ME and normal human skin (NHS). To confirm findings, affinity‐purified anti‐BP180‐NC16A/BP230 polyclonal Abs and recombinant anti‐BP180‐NC16A/BP230 monoclonal antibodies (mAbs) were used. Results: For sensitive detection of BP180‐NC16A‐specific IgG Abs, sections of NHS are superior to the widely used ME. Confirmation comes from polyclonal affinity‐purified anti‐BP180‐NC16A/BP230 Abs, and by mAbs cloned from a patient with active BP. Conclusions: Use of NHS is preferable over ME in routine IIF testing for BP. These results are of clinical relevance because anti‐BP180‐NC16A IgG titres are correlated to disease activity and detecting them reliably is important for screening, diagnosis and follow‐up of patients with BP. What's already known about this topic?Bullous pemphigoid (BP)180‐noncollagenous domain 16A (NC16A) is the immunodominant epitope in patients with BP, and anti‐BP180‐NC16A autoantibody (Ab) titres correlate with disease activity.Monkey oesophagus (ME) substrate is the most widely used substrate for screening, diagnosis and follow‐up of patients with BP.Published data indicate rather low sensitivity of ME substrate in immunofluorescence testing for BP. What does this study add? Use of normal human skin is required to detect anti‐BP180‐NC16A Abs reliably in immunofluorescence testing of human sera.The human BP180‐NC16A domain is poorly, if at all, expressed in ME. What is the translational message? Appropriate immunofluorescence substrates should be used for routine diagnostic work‐up in BP.Use of ME for serological testing of other human autoimmune conditions should be critically re‐evaluated. Linked Comment: Yamagami. Br J Dermatol 2019; 180:980–981. Respond to this article [ABSTRACT FROM AUTHOR]

Published
2019
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40. A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid.

Author

Goletz, S., van Beek, N., Holtsche, M.M., Schmidt, E., Probst, C., Komorowski, L., Schlumberger, W., Fechner, K., Stöcker, W., Recke, A., Zillikens, D., Yancey, K.B., Hashimoto, T., Antonicelli, F., and Di Zenzo, G.

Abstract

Summary: Background: Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement and autoantibodies against laminin 332. Malignancies have been associated with this disease; however, no standardized detection system for antilaminin 332 serum antibodies is widely available. Objectives: Development of a sensitive and specific assay for the detection of antilaminin 332 antibodies. Methods: An indirect immunofluorescence (IF) assay using recombinant laminin 332 was developed and probed with a large number of antilaminin 332 MMP patient sera (n = 93), as well as sera from patients with antilaminin 332‐negative MMP (n = 153), bullous pemphigoid (n = 20), pemphigus vulgaris (n = 20) and noninflammatory dermatoses (n = 22), and healthy blood donors (n = 100). Results: In the novel IF assay, sensitivities with the laminin 332 heterotrimer and the individual α3, β3 and γ2 chains were 77%, 43%, 41% and 13%, respectively, with specificities of 100% for each substrate. The sensitivity for the heterotrimer increased when an anti‐IgG4 enriched antitotal IgG conjugate was applied. Antilaminin 332 reactivity paralleled disease activity and was associated with malignancies in 25% of patients with antilaminin 332 MMP. Conclusions: The novel IF‐based assay will facilitate the serological diagnosis of antilaminin 332 MMP and may help to identify patients at risk of a malignancy. What's already known about this topic? Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement characterized by antilaminin 332 autoantibodies.Malignancies have been associated with this disease, but currently no standardized detection system for antilaminin 332 serum antibodies is widely available. What does this study add? A sensitive and highly specific immunofluorescence assay for the detection of antilaminin 332 autoantibodies using recombinant laminin 332‐expressing human cells was developed, which will soon be widely available. What is the translational message? This assay may not only facilitate the diagnosis of antilaminin 332 MMP but may also be valuable to monitor disease activity and identify patients at risk of a malignancy. Respond to this article [ABSTRACT FROM AUTHOR]

Published
2019
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42. Autoreactive Iga Antibodies against the Pancreatic Major Glycoprotein 2 are Associated with Primary Sclerosing Cholangitis and Related Biliary Tract Cancer

Author

Jendrek, S.T., primary, Gotthardt, D., additional, Nitzsche, T., additional, Korf, T., additional, Michaels, M.A., additional, Weiss, K.-H., additional, Liaskou, E., additional, Vesterhus, M., additional, Karlsen, T.H., additional, Mindorf, S., additional, Schemmer, P., additional, Bär, F., additional, Schröder, T., additional, Ehlers, M., additional, Hammers, C.M., additional, Komorowski, L., additional, Lehnert, H., additional, Fellermann, K., additional, Derer, S., additional, Hov, J.R., additional, and Sina, C., additional

Published
2016
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43. Neuronal Na+/K+ ATPase is an autoantibody target in paraneoplastic neurologic syndrome

Author

Scharf, M., primary, Miske, R., additional, Heidenreich, F., additional, Giess, R., additional, Landwehr, P., additional, Blocker, I.-M., additional, Begemann, N., additional, Denno, Y., additional, Tiede, S., additional, Dahnrich, C., additional, Schlumberger, W., additional, Unger, M., additional, Teegen, B., additional, Stocker, W., additional, Probst, C., additional, and Komorowski, L., additional

Published
2015
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46. DPPX potassium channel antibody: Frequency, clinical accompaniments, and outcomes in 20 patients

Author

Tobin, W. O., primary, Lennon, V. A., additional, Komorowski, L., additional, Probst, C., additional, Clardy, S. L., additional, Aksamit, A. J., additional, Appendino, J. P., additional, Lucchinetti, C. F., additional, Matsumoto, J. Y., additional, Pittock, S. J., additional, Sandroni, P., additional, Tippmann-Peikert, M., additional, Wirrell, E. C., additional, and McKeon, A., additional

Published
2014
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48. Development of a standardized ELISA for the determination of autoantibodies against human M-type phospholipase A2 receptor in primary membranous nephropathy

Author

Dahnrich, C., Komorowski, L., Probst, C., Seitz-Polski, B., Esnault, V., Wetzels, J.F.M., Hofstra, J.M., Hoxha, E., Stahl, R.A.K., Lambeau, G., Stocker, W., Schlumberger, W., Dahnrich, C., Komorowski, L., Probst, C., Seitz-Polski, B., Esnault, V., Wetzels, J.F.M., Hofstra, J.M., Hoxha, E., Stahl, R.A.K., Lambeau, G., Stocker, W., and Schlumberger, W.

Abstract

Item does not contain fulltext, BACKGROUND: Autoantibodies against the M-type phospholipase A2 receptor (PLA2R1) are specific markers for primary membranous nephropathy (pMN) and anti-PLA2R1 serum levels may be useful to monitor disease activity. So far, a recombinant cell-based indirect immunofluorescence assay (RC-IFA) using recombinant PLA2R1 as a substrate has been widely available but lacks a finely graduated assessment of antibody concentrations. METHODS: In order to setup a standardized ELISA, the extracellular domain of human PLA2R1 was expressed in HEK293. The purified protein was used to form the solid-phase in an ELISA which was then employed to analyze sera from 200 patients with primary MN, 27 patients with secondary MN, 230 patients with other glomerular diseases, 316 patients with systemic autoimmune diseases, and from 291 healthy blood donors. RESULTS: At a set specificity of 99.9% the sensitivity of the anti-PLA2R1 IgG ELISA was found to be 96.5%. A similar sensitivity (98.5%) was obtained when binding of only subclass IgG4 was analyzed. The calibrated assay showed a good class correlation with the results of the RC-IFA, was robust and could be stored for several months without any loss of quality. CONCLUSION: The results demonstrate that the new test system is qualified for routine use and that it has an almost perfect agreement with both, the clinical characterization of the patients and the results generated with RC-IFA.

Published
2013

49. SAT-387 - Autoreactive Iga Antibodies against the Pancreatic Major Glycoprotein 2 are Associated with Primary Sclerosing Cholangitis and Related Biliary Tract Cancer

Author

Jendrek, S.T., Gotthardt, D., Nitzsche, T., Korf, T., Michaels, M.A., Weiss, K.-H., Liaskou, E., Vesterhus, M., Karlsen, T.H., Mindorf, S., Schemmer, P., Bär, F., Schröder, T., Ehlers, M., Hammers, C.M., Komorowski, L., Lehnert, H., Fellermann, K., Derer, S., Hov, J.R., and Sina, C.

Published
2016
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