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27 results on '"Komatsu, Yumiko"'

8. Eel Serum Synergistically Enhances Growth of Fish Cell Line GAKS

Author

TAKUMI Shota, KOMATSU Yumiko, DOMEN Yasuhito, SUMIZAWA Tomoyuki, SHIOZAKI Kazuhiro, ISHIKAWA Manabu, KOMATSU Masaharu, HAYASHI Seiichi, and YAMADA Shoji

Subjects
cell culture, eel serum, fish cell line, GAKS
Abstract

Generation of cell lines derived from fish is getting to increase. Generally, mammalian cell line culture needs appropriate media with approximately 5 to 10 % fetal calf serum. However, the optimal culture conditions for fish cell lines have not been established so far. We investigated the effect of exposure to inactivated fish serum on cell culture of goldfish scale cell line GAKS. The 5 % sera from gibel, carp, tilapia, and eel were toxic to GAKS cells. In addition, these fish serum were toxic to not only the GAKS, but also the mammalian cell lines HEK293 and SH-SY5Y. GAKS cells did not proliferate under the culture condition with 5% fish serum. Although it is known that fish serum contains thiaminase, the effect of supplemental addition of vitamin B1 was negligible for the proliferation of GAKS cells. Lower concentrations of fish sera, especially less than 5 % of eel serum stimulated the cell survival of GAKS compared with 5 % of each fish serum. The culture medium containing around 1 % eel serum coupled with 1% FCS was comparable to 5 % FCS conditions for proliferation of GAKS cells. Furthermore, the passage culture was successful under these culture conditions.

Published
2021

15. Safety and tolerability of diazoxide in Japanese patients with hyperinsulinemic hypoglycemia

Author

Komatsu, Yumiko, 1000070552420, Nakamura, Akinobu, Takihata, Masahiro, Inoue, Yuichiro, Yahagi, Satoko, Tajima, Kazuki, Tsuchiya, Hirohisa, Takano, Tatsuro, Yamakawa, Tadashi, Yoshida, Masahiro, 1000030360902, Miyoshi, Hideaki, Terauchi, Yasuo, Komatsu, Yumiko, 1000070552420, Nakamura, Akinobu, Takihata, Masahiro, Inoue, Yuichiro, Yahagi, Satoko, Tajima, Kazuki, Tsuchiya, Hirohisa, Takano, Tatsuro, Yamakawa, Tadashi, Yoshida, Masahiro, 1000030360902, Miyoshi, Hideaki, and Terauchi, Yasuo

Abstract

Diazoxide is a non-diuretic benzothiadiazine derivative, one of a group of substances introduced into clinical practice in the 1950s for the treatment of hypertension. Fajans reported the use of diazoxide for the treatment of insulinoma in 1979. Although patients with hyperinsulinemic hypoglycemia worldwide have been treated with diazoxide for more than 30 years, there are no recent reports about the adverse effects of this drug in Asian patients, including Japanese patients. Herein, we report the results of our retrospective clinical record review of 6 Japanese patients (3 females and 3 males, ranging in age from 58 to 91 years) with hyperinsulinemic hypoglycemia and inoperable insulinoma treated with diazoxide. Diazoxide improved control of hypoglycemic symptoms and maintained normoglycemia in 5 of the 6 patients, and was ineffective in one patient. Surprisingly, although all 6 patients received diazoxide according to the treatment strategy recommended in Western patients, 5 of the 6 patients developed edema and two developed congestive heart failure. Thus, when starting treatment with diazoxide in Japanese patients, the symptoms and signs of fluid retention should be evaluated carefully. Also, appropriate protocols for treatment with diazoxide should be evaluated by means of clinical trials in Japanese patients with hyperinsulinemic hypoglycemia.

Published
2016

17. Safety and tolerability of diazoxide in Japanese patients with hyperinsulinemic hypoglycemia

Author

Komatsu, Yumiko, primary, Nakamura, Akinobu, additional, Takihata, Masahiro, additional, Inoue, Yuichiro, additional, Yahagi, Satoko, additional, Tajima, Kazuki, additional, Tsuchiya, Hirohisa, additional, Takano, Tatsuro, additional, Yamakawa, Tadashi, additional, Yoshida, Masahiro, additional, Miyoshi, Hideaki, additional, and Terauchi, Yasuo, additional

Published
2016
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23. Deletions and epimutations affecting the human 14q32.2 imprinted region in individuals with paternal and maternal upd(14)-like phenotypes

Author

Kagami, Masayo, primary, Sekita, Yoichi, additional, Nishimura, Gen, additional, Irie, Masahito, additional, Kato, Fumiko, additional, Okada, Michiyo, additional, Yamamori, Shunji, additional, Kishimoto, Hiroshi, additional, Nakayama, Masahiro, additional, Tanaka, Yukichi, additional, Matsuoka, Kentarou, additional, Takahashi, Tsutomu, additional, Noguchi, Mika, additional, Tanaka, Yoko, additional, Masumoto, Kouji, additional, Utsunomiya, Takeshi, additional, Kouzan, Hiroko, additional, Komatsu, Yumiko, additional, Ohashi, Hirofumi, additional, Kurosawa, Kenji, additional, Kosaki, Kenjirou, additional, Ferguson-Smith, Anne C, additional, Ishino, Fumitoshi, additional, and Ogata, Tsutomu, additional

Published
2008
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25. Factors associated with antisocial behavior in patients with developmental disorder.

Author

Nakamura Y, Shimazaki M, Komatsu Y, Nakano Y, Matsuoka Y, Miyata, and Oka A

Subjects
Adolescent, Child, Child, Preschool, Female, Humans, Male, Social Behavior, Antisocial Personality Disorder, Developmental Disabilities
Abstract

Objective: This study investigated the factors associated with antisocial behavior (AB) in children with developmental disorder and effective treatments. Methods: Participants were 110 schoolchildren with developmental disorder and with or without accompanying AB who visited our hospital between October 2009 and October 2012. Among the children with AB, those who exhibited one or more symptoms of conduct disorder (CD) were assigned to the CD subgroup. We examined the background characteristics, past history, type of antisocial behavior, and symptom improvement after treatment in the children with AB and compared the relevant factors with children with developmental disorder without AB. Results: Of the 110 participants, 72 (65.5%) did not exhibit AB and 38 (34.5%) did, 7 (5.5%) of whom fulfilled the criteria for CD. Compared to the children without AB, the children with AB showed a significantly higher occurrence of attention deficit/hyperactivity disorder (AD/HD), maltreatment, institutionalization due to maltreatment, parental mental/psychological problems, and family instability. After medical treatment combined with social-skills training and parental education, 22 of the 38 children with AB showed improved behavior. In the CD subgroup, 4 children were diagnosed with AD/HD and 3 with pervasive developmental disorder, and none of the 7 improved with treatment. Conclusion: AB was associated with AD/HD, maltreatment, institutionalization, parental mental/psychological problems, and family instability. The most effective therapy was parental education. Children with AB need early intervention given that those who already exhibited symptoms of CD showed little improvement with treatment.

Published
2016

26. [A case of large arteriovenous malformation in the frontal lobe complicating attention-deficit/hyperactivity disorder].

Author

Nakamura Y, Shimazaki M, Komatsu Y, Miwa M, Bessho F, and Oka A

Subjects
Arteriovenous Malformations pathology, Arteriovenous Malformations surgery, Child, Preschool, Humans, Magnetic Resonance Imaging, Male, Tomography, X-Ray Computed, Arteriovenous Malformations complications, Attention Deficit Disorder with Hyperactivity etiology, Frontal Lobe pathology
Abstract

We report a 4-year old boy with a large arteriovenous malformation (AVM) exhibiting attention-deficit hyperactivity disorder (AD/HD). He presented with hyperkinesis at the age of 3 years and jacksonian seizure at 3 years 11 months, when he was diagnosed as AVM by cranial computed tomography. Magnetic resonance imaging revealed an AVM of 6 cm in diameter in the left frontal lobe. After 1 year, the AVM developed a varix, and both were surgically removed. We speculate that the prefrontal area was affected by direct compression from AVM and chronic ischemia due to steal phenomenon. Although AD/HD is rarely caused by parenchymal lesions, such as AVM, physicians should carefully investigate causative lesions.

Published
2014

27. [Nonconvulsive status epilepticus as an initial symptom in a boy with frontal lobe epilepsy].

Author

Miyata Y, Shimazaki M, Komatsu Y, Nakamura Y, and Oka A

Subjects
Anticonvulsants therapeutic use, Child, Diazepam therapeutic use, Electroencephalography, Epilepsy, Frontal Lobe drug therapy, Epilepsy, Frontal Lobe physiopathology, Humans, Male, Treatment Outcome, Epilepsy, Frontal Lobe complications, Status Epilepticus etiology
Abstract

An 11-year-old boy, who had no remarkable past history, exhibited disorientation and abnormal behavior lasting for several hours. Continuous ictal discharges on his EEG lead to the diagnosis of nonconvulsive status epilepticus (NCSE). The administration of diazepam instantly resulted in the cessation of ictal discharges, associated with clinical recovery. Interictal spikes distributed in frontal lobes are sporadically seen, suggesting frontal lobe as an epileptic focus. After starting medication, he showed excellent clinical course without recurrence of seizure or neurological sequelae. Although NCSE is generally suggestive of poor prognosis, some subtypes of NCSE, such as partial status epilepticus and absence status epilepticus, are not always associated with adverse outcome. The present case suggests that epileptic patients who present NCSE at onset and lack interictal neurological impairments might have good outcome.

Published
2014

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