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The structural elucidation of lipopolysaccharides (LPSs) from Gram‐negative marine bacteria, along with the assessment of their immunological properties, is a fascinating and active research field. Such studies can aid understanding of adaptation phenomena that occur in the marine environment, but they can also open up new perspectives on the design and development of new immunoregulatory drugs. In this paper, we report the structural characterization of the lipid A component of the LPS isolated from the marine bacterium Cobetia pacifica KMM 3879T, which is characterized by a family of structures differing in their acylation patterns. The structural assignment was achieved through extensive chemical analysis and matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry. Moreover, cellular immunology studies on the LPS highlighted its low immunostimulatory impact, as well as a very interesting and promising inhibitory activity of the toxic effects of Escherichia coli LPS. [ABSTRACT FROM AUTHOR]

The O-specific polysaccharide was isolated from the lipopolysaccharide of Rheinheimera pacifica KMM 1406T and studied by chemical methods along with 1H and 13C NMR spectroscopy. It was shown that the polysaccharide contains one residue each of 2-acetamido-2-deoxy- galactose (GalNAc), 2-acetamido-2-deoxy- and 2-acetamido-2-deoxy-galacturonic acids (GalNAcA, GalNAcA), 2,4-diacetamido-2,4,6-trideoxy- glucose (QuiNAc4NAc), and 4-(N-acetyl- alanyl)amino-4,6-dideoxy- glucose (Qui4NAlaAc) and has the following structure: (→4)-α- GalpNAc-(1→4)-α- GalpNAcA-(1→3)-β- QuipNAc4NAc-(1→2)-β- Quip4NDAlaAc-(1→4)-α- GalpNAcA-(1→) [ABSTRACT FROM AUTHOR]

Abstract: The O-specific polysaccharide was isolated from the lipopolysaccharide of a marine bacterium Oceanisphaera litoralis KMM 3654T and studied by chemical methods along with 1H and 13C NMR spectroscopy. The following new structure of the O-specific polysaccharide of O. litoralis containing d-glucose and two residues of 2-acetamido-2-deoxy-d-mannuronic acid was established: Display Omitted [Copyright &y& Elsevier]

Lipopolysaccharide from pathogenic Yersinia pseudotuberculosis type III, strain 209, has been isolated and characterized. Lipopolysaccharide was shown to contain lipid A (30%), protein (4.4%), 2-keto-3-deoxyoctonate (7.8%) and a polysaccharide component (45%). which consisted of paratose, L-fucose, D-mannose, D-glucose, D-galactose (trace), L- and D-glycero-D-manno-heptose, 2-deoxy-2-amino-D-glucose and 2-deoxy-2-amino-D-galactose residues. Molecular-sieve chromatography on Sephadex G-50 afforded an O-specific polysaccharide, being hydrolysed to yield equimolar amounts of paratose, L-fucose, D-mannose and 2-deoxy-2-amino-D-galactose. The chemical repeating unit of the O-specific polysaccharide has been suggested on the basis of methylation studies and periodate oxidation data. [ABSTRACT FROM AUTHOR]

Highlights: [•] Structure of the O-polysaccharide of C. pacifica KMM 3879T was established. [•] The O-polysaccharide is built up of trisaccharide repeating units. [•] Sulfate groups were found in OPS. [ABSTRACT FROM AUTHOR]

Highlights: [•] The new structure of the O-polysaccharide of L. taeanensis G5T is reported. [•] The simultaneous presence of l-Sug and d-QuiNAc4NR was found in OPS. [•] The configuration of the 3,5-dihydroxyhexanoic acid was established by Mosher esters. [Copyright &y& Elsevier]

The structural elucidation of lipopolysaccharides (LPSs) from Gram‐negative marine bacteria, along with the assessment of their immunological properties, is a fascinating and active research field. Such studies can aid understanding of adaptation phenomena that occur in the marine environment, but they can also open up new perspectives on the design and development of new immunoregulatory drugs. In this paper, we report the structural characterization of the lipid A component of the LPS isolated from the marine bacterium Cobetia pacifica KMM 3879T, which is characterized by a family of structures differing in their acylation patterns. The structural assignment was achieved through extensive chemical analysis and matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry. Moreover, cellular immunology studies on the LPS highlighted its low immunostimulatory impact, as well as a very interesting and promising inhibitory activity of the toxic effects of Escherichia coli LPS. [ABSTRACT FROM AUTHOR]

Mild acid degradation of the lipopolysaccharide of the bacterium Idiomarina zobellii, type strain KMM 231T, with aq 2% HOAc at 100 °C, yielded an oligosaccharide, which represents one repeating unit of the O-polysaccharide. A polysaccharide was obtained by mild base degradation of the lipopolysaccharide. The following structure of the O-polysaccharide was elucidated by 1H and 13C NMR spectroscopy of the oligosaccharide and base-degraded lipopolysaccharide, including COSY, TOCSY, ROESY, 1H, 13C HSQC, HSQC-TOCSY and HMBC experiments:→ 3)-α-d-Quip4N-(1 → 4)-α-d-GlcpA-(1 → 6)-α-d-GlcpNAc-(1 → 4)-α-l-GulpNA-(1 → 3)-β-d-FucpNAc-(1 →The O-polysaccharide is distinguished by the presence of two unusual amino sugars, 4-amino-4,6-dideoxy-d-glucose (d-Qui4N) and 2-amino-2-deoxy-l-guluronic acid (l-GulNA), both having the free amino group. The unexpectedly high acid lability of the glycosidic linkage of 2-acetamido-2,6-dideoxy-d-galactose (d-FucNAc) could be associated with the presence of a free amino group adjacent to the site of attachment of FucNAc to Qui4N. [Copyright &y& Elsevier]