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High‐depth whole‐genome resequencing of 53 diverse fig tree genotypes yielded a rich dataset of genetic variants. We successfully identified 5,501,460 single‐nucleotide polymorphisms (SNPs) and 1,228,537 insertions and deletions (InDels), providing a high‐density and excellent‐quality genetic map of the fig tree. We also performed a detailed population structure analysis, dividing the 53 genotypes into three geographical groups and assessing their genetic diversity and divergence. Analysis of structural variants (SVs) and copy number variations (CNVs) revealed their potential functional impact, particularly in plant‐pathogen interaction and secondary metabolism. Metabolomic fingerprinting of fig genotypes uncovered extensive variation in primary metabolites and polyphenolic compounds, highlighting the influence of genotype on fruit quality traits such as nutritional content and bioactive compound composition. The genome‐wide association study (GWAS) identified critical SNPs associated with fruit quality and morphological features. The discovery of significant candidate genes, such as AGL62, GDSL, and COBRA‐like protein 4 genes, offers promising targets for marker‐assisted selection and genome editing approaches to improve fig fruit morphological and quality traits. This extensive genomic analysis of fig trees enhances our understanding of the genetic basis of important agronomic traits and provides a rich resource for future research in this economically and nutritionally significant fruit. [ABSTRACT FROM AUTHOR]

Zero-discharge and low-input aquaponics systems are a promising alternative to the intensive agricultural and aquacultural production systems currently used, ensuring high environmental sustainability. However, new approaches and management practices are needed to increase their productivity to reach the yields of classic production systems. In this context, the present study investigated for the first time the potential of two biostimulants to improve lettuce performance in aquaponics, whether coupled or decoupled, with hydroponics serving as a control. A comprehensive evaluation was conducted to assess the plant functional (focusing on the photosynthetic process evaluation) and growth responses at the whole-plant level. In addition, the nutritional state of the leaves was determined and metabolomic analysis was performed at the cellular level, the latter also for the first time in aquaponics research. The results demonstrated the limitations that coupled aquaponics poses in relation to lettuce growth, function and metabolism, which were already obvious from the 12th day of the experiment. Indicatively, the plants grown under coupled aquaponics exhibited a notable decrease in the leaf fresh weight, potassium content and nitrogen content, with reductions of 80%, 60%, and 30%, respectively, in comparison to the hydroponics control. However, the combined physiological and metabolomic data indicate that these plants down-regulate processes and metabolism to acclimate to low nutrient levels in lettuce leaves rather than experiencing damage. The application of biostimulants did not significantly optimize the plants' performance, though one of them appeared to be effective in improving some aspects of the photochemical efficiency. The decoupled and hydroponics systems resulted in similarly high yields and efficiency in terms of plant function, without any marked contribution from the biostimulants. We conclude that the decoupled aquaponics system has been successful in achieving yields comparable to those of hydroponics, with lower chemical inputs. Future studies should focus on examining other biostimulants in this system to further improve its performance while maintaining its environmental benefits within a circular economy framework. [ABSTRACT FROM AUTHOR]