Search

Objective: To evaluate the antidiabetic, antihyperlipidemic and in vivo antioxidant potential of aqueous extract of Anogeissus latifolia bark (AEALB) in streptozotocin-nicotinamide (STZ-NIN)- induced type 2 diabetic rats.Methods: Oral acute toxicity for AEALB was performed in rats at 2 g/kg dose. In rats, type 2 diabetes was induced by administration of STZ-NIN (65 mg/kg-110 mg/kg, i.p). AEALB 100, 200 mg/kg dose was administered orally to the diabetic rats up to four weeks. The body weight and blood glucose levels were determined at the end of every week. The haemoglobin, glycosylated haemoglobin, serum lipid profiles and other biochemical parameters were measured on fourth week. The antioxidants level in liver, kidney and pancreas of diabetic rats treated with AEALB were estimated. Results: In the acute toxicity study, AEALB was nontoxic at 2 g/kg in rats. The increased body weight, haemoglobin and decreased blood glucose, glycosylated haemoglobin and other biochemical parameters level was observed in diabetic rats treated with both doses of AEALB compared to diabetic control rats. In diabetic rats, AEALB administration, altered lipid profiles and antioxidants level were reversed to near normal than diabetic control rats. Conclusion: Aqueous extract of Anogeissus latifolia bark possess significant antidiabetic, antihyperlipidemic and in vivo antioxidant activity in type 2 diabetic rats.

Background: Abelmoschus esculentus (L.) Moench. fruit is a commonly consumed vegetable in many countries due to its rich medicinal value. However, till date, in vivo antioxidant property of A. esculentus has not been scientifically documented in animal models. Objective: The present investigation was aimed to evaluate the in vivo antioxidant property of A. esculentus (L.) Moench. peel and seed powder (AEPP and AESP) in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: In rats, acute toxicity assessment of AEPP and AESP at 2 g/kg did not show any toxicity. Diabetes was induced by STZ (60 mg/kg, i.p.) injection and diabetic rats received AEPP (100 and 200 mg/kg) as well as AESP (100 and 200 mg/ kg) orally up to 28 days. At the end of the 28 day, diabetic rats were killed and liver, kidney and pancreas were collected to determine superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and lipid peroxidation level. Results: In diabetic rats, significant (P < 0.001) reduction of liver, kidney and pancreas SOD, CAT, GPx, GSH levels and increase in thiobarbituric acid reactive substances (TBARS) were observed as compared to normal control rats. Administration of both doses of AEPP and AESP significantly (P < 0.001 and P < 0.01) increased liver, kidney and pancreas SOD, CAT, GPx, GSH levels and decreased TBARS (P < 0.001) levels in diabetic rats compared to diabetic control rats. Conclusion: Our findings confirmed that A. esculentus peel and seed powder has significant in vivo antioxidant property in diabetic rats.