77 results on '"Kohorst M"'
Search Results
2. Improved detection of sinusoidal obstructive syndrome using pediatric–AYA diagnostic criteria and severity grading
- Author
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Ragoonanan, D., Khazal, S. J., Wang, J., Payne, A., Kohorst, M., Harden, A., Tewari, P., Petropoulos, D., Shoberu, B., Kebriaei, P., Mahadeo, K. M., and Tambaro, F. P.
- Published
- 2021
- Full Text
- View/download PDF
3. Attempted desensitization to calaspargase pegol: a familiar approach to a new problem.
- Author
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Kuhn AK, Martin C, Galardy P, McCullough K, Greenmyer J, O'Keefe M, Walter A, Thompson C, Ferdjallah A, Kalmes J, and Kohorst M
- Subjects
- Humans, Polyethylene Glycols adverse effects, Asparaginase, Drug Hypersensitivity diagnosis, Drug Hypersensitivity etiology, Drug Hypersensitivity therapy
- Published
- 2024
- Full Text
- View/download PDF
4. Profound Hypoglycemia and High Anion Gap Metabolic Acidosis in a Pediatric Leukemic Patient Receiving 6-Mercaptopurine.
- Author
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O'Shea M, Kuhn A, Creo AL, Kohorst M, and Ferdjallah A
- Abstract
A 13-year-old male undergoing maintenance chemotherapy with methotrexate and 6-mercaptopurine (6MP), for very high-risk B-cell acute lymphoblastic leukemia (ALL), presented with vomiting due to severe hypoglycemia with metabolic acidosis. While his laboratory values were concerning for a critically ill child, the patient was relatively well appearing. Hypoglycemia is a rare but serious side effect of 6MP with an unexpectedly variable presentation; therefore, a high index of suspicion is needed for its prompt detection and treatment. This patient also had severe metabolic acidosis, likely secondary to hypoglycemia, creating a serious clinical picture despite a well-appearing child. This example of incongruity between laboratory tests and clinical appearance adds nuance to the existing literature. Moreover, although 6MP-associated hypoglycemia is rare, it may be more prevalent than the literature suggests, as symptoms of hypoglycemia-nausea, vomiting, and somnolence-mirror common chemotherapy side effects. 6MP-induced hypoglycemia can be ameliorated with the addition of allopurinol to shunt metabolism in favor of the production of therapeutic metabolites over hepatotoxic metabolites. Additionally, a morning administration of 6MP and frequent snacks may also help to prevent hypoglycemia. Overall, this case adds to the literature of unusual reactions to 6MP including hypoglycemia in an older child without traditional risk factors.
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- 2024
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5. Determinants of outcomes and advances in CD19-directed chimeric antigen receptor therapy for B-cell acute lymphoblastic leukemia.
- Author
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Gupta S, Kohorst M, and Alkhateeb HB
- Subjects
- Humans, Antigens, CD19, Immunotherapy, Adoptive adverse effects, Receptors, Antigen, T-Cell genetics, Recurrence, Burkitt Lymphoma drug therapy, Lymphoma, B-Cell drug therapy, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Receptors, Chimeric Antigen genetics
- Abstract
Relapsed and refractory B-cell acute lymphoblastic leukemia (B-ALL) is an aggressive B-cell neoplasm associated with poor outcomes. Conventional multiagent chemotherapy and bispecific antibody therapy may induce remission; however, relapse rates remain high and overall survival is poor. Chimeric antigen receptor T-cell (CAR-T) therapy provides durable, deep complete remission, and long-term cures in relapsed and refractory B-ALL. However, with this new treatment modality, 10%-30% of patients do not achieve remission, and over 50% experience relapse after therapy. Currently, there are two approved CD19-specific CAR-T cell constructs in B-ALL, Tisagenlecleucel and Brexucabtagene Autoleucel by the United States Food and Drug Administration, and the European Medicines Agency (EMA). In this review, we discuss patients, disease, and CAR-T predictors of outcomes in B-ALL. We describe the two approved CD19-directed CAR-T cell products, review the current literature, and discuss factors associated with high risks of therapy failure and future direction in CAR-T cell therapy for B-ALL., (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2024
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6. Autoimmune cytopenias following pediatric hematopoietic cell transplant.
- Author
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Greenmyer JR, Ariagno S, Ali A, Pence L, O'Shea M, Greenmyer LA, Khan S, Kuhn A, Martin C, Ferdjallah A, and Kohorst M
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- Humans, Child, Hematopoietic Stem Cell Transplantation adverse effects, Cytopenia, Thrombocytopenia, Anemia, Transplants, Anemia, Hemolytic, Autoimmune etiology
- Published
- 2024
- Full Text
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7. Evaluation of Children with Malignancies for Blood and Marrow Transplantation: A Report from the ASTCT Committee on Practice Guidelines.
- Author
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Fraint E, Abdel-Azim H, Bhatt NS, Broglie L, Chattha A, Kohorst M, Ktena YP, Lee MA, Long S, Qayed M, Sharma A, Stefanski H, Vatsayan A, Wray L, Hamadani M, and Carpenter PA
- Subjects
- Adult, Child, Humans, United States, Bone Marrow, Transplantation, Homologous, Research Report, Hematopoietic Stem Cell Transplantation, Neoplasms therapy
- Abstract
Evaluation of a candidate for hematopoietic cell transplantation (HCT) is a complex process with substantial intercenter variability. Although literature providing guidance for evaluating the eligibility of adults is well established, similar guidance for children is lacking. To address gaps between adult recommendations and the specific needs of children, we convened a panel of pediatric HCT experts from a wide geographic range of American Society of Transplantation and Cellular Therapy (ASTCT) member institutions to offer recommendations for pediatric-focused pre-HCT evaluation. In this report from the ASTCT Committee on Practice Guidelines, we present a practical framework for evaluating children with malignancies who are candidates for HCT. We also highlight key differences from adults and emphasize areas of unmet need that require additional research to delineate best practices., (Copyright © 2023 The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
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- 2023
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8. Letermovir as Cytomegalovirus Prophylaxis in a Pediatric Cohort: A Retrospective Analysis.
- Author
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Kuhn A, Puttkammer J, Madigan T, Dinnes L, Khan S, Ferdjallah A, and Kohorst M
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- Female, Humans, Child, Young Adult, Adult, Child, Preschool, Adolescent, Antiviral Agents therapeutic use, Antiviral Agents adverse effects, Retrospective Studies, Valganciclovir pharmacology, Valganciclovir therapeutic use, Cytomegalovirus, Cytomegalovirus Infections drug therapy, Cytomegalovirus Infections prevention & control
- Abstract
Letermovir is an attractive cytomegalovirus (CMV) prophylactic agent, but published data in children are scarce. This retrospective chart review aimed to describe our experience using letermovir as CMV prophylaxis in pediatric hematopoietic cell transplantation (HCT) recipients. Pediatric patients (age <20 years) undergoing allogeneic HCT and receiving letermovir prophylaxis in the Mayo Clinic Pediatric Bone Marrow Transplant Program were eligible for inclusion in this retrospective chart review. Medical records were reviewed to evaluate letermovir dosing, CMV levels, laboratory values, and reports of adverse effects. Between October 2020 and April 2022, 9 patients age 4 to 19 years undergoing allogeneic HCT in the Pediatric Bone Marrow Transplant Program received letermovir prophylaxis, either 240 mg or 480 mg daily at a mean and median dose of 10 mg/kg/day. Letermovir was crushed and administered via nasogastric tube in 4 of 9 patients. Two patients received letermovir for secondary CMV prophylaxis after initial treatment with ganciclovir/valganciclovir, and the remaining 7 received letermovir for primary prophylaxis. One patient, a 20-kg 6-year-old female receiving 240 mg (12 mg/kg), experienced low-level CMV viremia while on letermovir. No other patients experienced CMV reactivation while on letermovir prophylaxis. In 2 patients, transient mild transaminitis was noted within the first weeks of letermovir therapy, which resolved without intervention, and its relationship to letermovir could not be clearly established. Letermovir administration was feasible and well tolerated as CMV prophylaxis in our small cohort of pediatric patients undergoing HCT. Larger, prospective studies are warranted to confirm the safety and efficacy of letermovir in children. © 2022 American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc., Competing Interests: Declaration of Competing Interest There are no conflicts of interest to report., (Copyright © 2022 The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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9. Improved detection of sinusoidal obstructive syndrome using pediatric–AYA diagnostic criteria and severity grading
- Author
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Ragoonanan, D., primary, Khazal, S. J., additional, Wang, J., additional, Payne, A., additional, Kohorst, M., additional, Harden, A., additional, Tewari, P., additional, Petropoulos, D., additional, Shoberu, B., additional, Kebriaei, P., additional, Mahadeo, K. M., additional, and Tambaro, F. P., additional
- Published
- 2020
- Full Text
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10. Cellular variant of kaposiform lymphangiomatosis: a report of three cases, expanding the morphologic and molecular genetic spectrum of this rare entity.
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Allen-Rhoades W, Al-Ibraheemi A, Kohorst M, Tollefson M, Hull N, Polites S, and Folpe AL
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- Child, Child, Preschool, Endothelial Cells pathology, Female, Humans, Male, Molecular Biology, Young Adult, Hemangioendothelioma genetics, Hemangioendothelioma pathology, Kasabach-Merritt Syndrome genetics, Kasabach-Merritt Syndrome pathology, Kasabach-Merritt Syndrome therapy, Sarcoma, Kaposi pathology
- Abstract
Kaposiform lymphangiomatosis (KLA) is a very rare form of generalized lymphatic anomaly, consisting of a diffuse proliferation of abnormal, dilated lymphatics, and small fascicles of hemosiderin-laden spindled lymphatic endothelial cells. KLA occurs in children and young adults and may present with multicentric disease, pleural and pericardial effusions, and life-threatening coagulopathy. Genetically, KLA most often harbors somatic activating mutations in NRAS. We recently encountered 3 cases of KLA with cellular features, resembling kaposiform hemangioendothelioma (KHE), and studied their clinicopathologic, radiologic, and molecular genetic features. The patients (1 male, 2 females; aged 2 years, 2 months, 4 years) presented with multicentric disease involving skin, soft tissue, bone, and spleen and thrombocytopenia/coagulopathy. Advanced imaging studies confirmed multicentric disease. Biopsies (skin, soft tissue, bone, and spleen) demonstrated both conventional KLA and much more cellular foci, consisting of sheets, nodules, glomeruloid structures, and sieve-like arrays of lymphatic endothelial cells (positive for CD31 and D2-40). Cellular areas superficially resembled KHE but displayed more epithelioid cytology and lacked surrounding hyaline fibrosis and minute platelet aggregates. Molecular genetic studies demonstrated NRAS c.181C > A p.Q61K (Gln61Lys) in 2 specimens from one patient and HRAS p.A59_Q61delinsGGSIL in another. Two patients were treated with sirolimus; all are currently alive with stable disease. We conclude that cellular morphology in KLA, a previously undescribed feature, does not appear to be associated with clinical features, site of disease, mutation type, response to sirolimus, or outcome. Although cellular KLA may mimic KHE, there are sufficient clinical, morphologic, and genetic differences such that these are likely unrelated diseases., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
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11. Pediatric Neoplasms Presenting with Monocytosis.
- Author
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Greenmyer JR and Kohorst M
- Subjects
- Biomarkers, Tumor, Combined Modality Therapy, Disease Management, Disease Susceptibility, Genetic Predisposition to Disease, Humans, Leukemia, Myelomonocytic, Juvenile etiology, Leukemia, Myelomonocytic, Juvenile metabolism, Leukemia, Myelomonocytic, Juvenile therapy, Mutation, Phenotype, Symptom Assessment, Leukemia, Myelomonocytic, Juvenile diagnosis
- Abstract
Purpose of Review: Juvenile myelomonocytic leukemia (JMML) is a rare but severe pediatric neoplasm with hematopoietic stem cell transplant as its only established curative option. The development of targeted therapeutics for JMML is being guided by an understanding of the pathobiology of this condition. Here, we review JMML with an emphasis on genetics in order to (i) demonstrate the relationship between JMML genotype and clinical phenotype and (ii) explore potential genetic targets of novel JMML therapies., Recent Findings: DNA hypermethylation studies have demonstrated consistently that methylation is related to disease severity. Increasing understanding of methylation in JMML may open the door to novel therapies, such as DNA methyltransferase inhibitors. The PI3K/AKT/MTOR, JAK/STAT, and RAF/MEK/ERK pathways are being investigated as therapeutic targets for JMML. Future therapy for JMML will be driven by an increased understanding of pathobiology. Targeted therapeutic approaches hold potential for improving outcomes in patients with JMML.
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- 2021
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12. Immune Effector Cell Associated Neurotoxicity (ICANS) in Pediatric and Young Adult Patients Following Chimeric Antigen Receptor (CAR) T-Cell Therapy: Can We Optimize Early Diagnosis?
- Author
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Brown BD, Tambaro FP, Kohorst M, Chi L, Mahadeo KM, Tewari P, Petropoulos D, Slopis JM, Sadighi Z, and Khazal S
- Abstract
The Cornell Assessment for Pediatric Delirium (CAPD) was first proposed by the Pediatric Acute Lung Injury and Sepsis Investigators Network-Stem Cell Transplantation and Cancer Immunotherapy Subgroup and MD Anderson CARTOX joint working committees, for detection of immune effector cell associated neurotoxicity (ICANS) in pediatric patients receiving chimeric antigen receptor (CAR) T-cell therapy. It was subsequently adopted by the American Society for Transplantation and Cellular Therapy. The utility of CAPD as a screening tool for early diagnosis of ICANS has not been fully characterized. We conducted a retrospective study of pediatric and young adult patients (n=15) receiving standard-of-care CAR T-cell products. Cytokine release syndrome (CRS) and ICANS occurred in 87% and 40% of patients, respectively. ICANS was associated with significantly higher peaks of serum ferritin. A change in CAPD from a prior baseline was noted in 60% of patients with ICANS, 24-72 h prior to diagnosis of ICANS. The median change from baseline to maximum CAPD score of patients who developed ICANS versus those who did not was 13 versus 3, respectively (p=0.0004). Changes in CAPD score from baseline may be the earliest indicator of ICANS among pediatric and young adult patients which may warrant closer monitoring, with more frequent CAPD assessments., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Brown, Tambaro, Kohorst, Chi, Mahadeo, Tewari, Petropoulos, Slopis, Sadighi and Khazal.)
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- 2021
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13. Lith-Entwicklung : die 'farbige Dimension' der Schwarzweissfotografie
- Author
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Kohorst, M.
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- 1999
- Full Text
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14. CAD speeds up dinnerware designs
- Author
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Sheppard, L., primary and Kohorst, M., additional
- Published
- 2002
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15. Haploidentical HSCT in the Treatment of Pediatric Hematological Disorders.
- Author
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Marszołek, Anna, Leśniak, Maria, Sekunda, Anna, Siwek, Aleksander, Skiba, Zuzanna, Lejman, Monika, and Zawitkowska, Joanna
- Subjects
BLOOD diseases ,PEDIATRIC therapy ,HEMATOPOIETIC stem cell transplantation ,MYCOSES ,BACTERIAL diseases ,PRIMARY immunodeficiency diseases ,HEMATOLOGIC malignancies - Abstract
Allogeneic hematopoietic stem cell transplantation has become a treatment option for otherwise non-curative conditions, both malignant and benign, affecting children and adults. Nevertheless, the latest research has been focusing extensively on transplantation from related and unrelated haploidentical donors, suitable for patients requiring emergent hematopoietic stem cell transplantation (HSCT) in the absence of an HLA-matched donor. Haploidentical HSCT (haplo-HSCT) can be an effective treatment for non-malignant pediatric disorders, such as primary immunodeficiencies or hemoglobinopathies, by enabling a much quicker selection of the appropriate donor for virtually all patients, low incidence of graft-versus-host disease (GVHD), and transplant-related mortality (TRM). Moreover, the outcomes of haplo-HSCT among children with hematological malignancies have improved radically. The most demanding tasks for clinicians are minimizing T-cell-mediated alloreactivity as well as early GVHD prevention. As a result, several T-cell depletion approaches, such as ex vivo T-cell depletion (TCD), and T-cell replete approaches, such as a combination of anti-thymocyte globulin (ATG), post-transplantation cyclophosphamide (PTCy), cyclosporine/tacrolimus, mycophenolate mofetil, or methotrexate, have been taken up. As more research is needed to establish the most beneficial form of therapy, haplo-HSCT is currently considered an alternative donor strategy for pediatric and adult patients with complications like viral and bacterial infections, invasive fungal disease, and GVHD. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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16. Case report: Rapid resolution of grade IV ICANS after first line intrathecal chemotherapy with methotrexate, cytarabine and dexamethasone.
- Author
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Katsin, Mikalai, Shman, Tatsiana, Migas, Alexandr, Lutskovich, Dzmitry, Serada, Yuliya, Khalankova, Yauheniya, Kostina, Yuliya, and Dubovik, Simon
- Subjects
METHOTREXATE ,IMMUNE reconstitution inflammatory syndrome ,CYTARABINE ,CANCER chemotherapy ,DEXAMETHASONE ,CHIMERIC antigen receptors - Abstract
Corticosteroid therapy is the mainstay of immune effector cell-associated neurotoxicity syndrome (ICANS) management, although its use has been associated with worse overall survival (OS) and progression-free survival (PFS) after chimeric antigen receptor T-cell (CAR-T cell) therapy. Many options are being investigated for prophylaxis and management. Accumulating evidence supports the use of intrathecal (IT) chemotherapy for the management of high-grade ICANS. Here, we describe a case of a patient with stage IV Primary mediastinal Bcell lymphoma (PMBCL) successfully treated with IT methotrexate, cytarabine, and dexamethasone as first-line therapy for CD19 CAR-T cell-associated grade IV ICANS. The stable and rapid resolution of ICANS to grade 0 allowed us to discontinue systemic corticosteroid use, avoiding CAR-T cells ablation and ensuring preservation of CAR-T cell function. The described patient achieved a complete radiologic and clinical response to CD19 CAR-T cell therapy and remains disease-free after 9 months. This case demonstrates a promising example of how IT chemotherapy could be used as first-line treatment for the management of high-grade ICANS. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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17. DDX41: exploring the roles of a versatile helicase.
- Author
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Winstone, Lacey, Yohan Jung, and Yuliang Wu
- Subjects
ACUTE myeloid leukemia ,RNA splicing ,HEMATOLOGIC malignancies ,MYELODYSPLASTIC syndromes ,ORGANELLE formation ,DRUG development - Abstract
DDX41 is a DEAD-box helicase and is conserved across species. Mutations in DDX41 have been associated with myeloid neoplasms, including myelodysplastic syndrome and acute myeloid leukemia. Though its pathogenesis is not completely known, DDX41 has been shown to have many cellular roles, including in pre-mRNA splicing, innate immune sensing, ribosome biogenesis, translational regulation, and R-loop metabolism. In this review, we will summarize the latest understandings regarding the various roles of DDX41, as well as highlight challenges associated with drug development to target DDX41. Overall, understanding the molecular and cellular mechanisms of DDX41 could help develop novel therapeutic options for DDX41 mutation-related hematologic malignancies. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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18. Risk assessment and genetic counseling for hematologic malignancies-Practice resource of the National Society of Genetic Counselors.
- Author
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Stewart BL, Helber H, Bannon SA, Deuitch NT, Ferguson M, Fiala E, Hamilton KV, Malcolmson J, Pencheva B, and Smith-Simmer K
- Abstract
Hematologic malignancies (HMs) are a heterogeneous group of cancers impacting individuals of all ages that have been increasingly recognized in association with various germline predisposition syndromes. Given the myriad of malignancy subtypes, expanding differential diagnoses, and unique sample selection requirements, evaluation for hereditary predisposition to HM presents both challenges as well as exciting opportunities in the ever-evolving field of genetic counseling. This practice resource has been developed as a foundational resource for genetic counseling approaches to hereditary HMs and aims to empower genetic counselors who encounter individuals and families with HMs in their practice., (© 2024 National Society of Genetic Counselors.)
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- 2024
- Full Text
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19. A novel 3q interstitial deletion including GATA2 and ZNF148: A case report.
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Martin E, VanSickle EA, and Rossetti LZ
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- Humans, Male, DNA-Binding Proteins genetics, Agenesis of Corpus Callosum genetics, Agenesis of Corpus Callosum pathology, Female, Developmental Disabilities genetics, Developmental Disabilities pathology, GATA2 Transcription Factor genetics, Chromosome Deletion, Chromosomes, Human, Pair 3 genetics, Transcription Factors genetics
- Abstract
GATA2 and ZNF148 have both been mapped to chromosome 3q. Pathogenic variants in GATA2 have been associated with immunodeficiency and high risk for myelodysplasia, acute myeloid leukemia, and chronic myelomonocytic leukemia. Gain-of-function variants in ZNF148 have previously been suggested as a mechanism for agenesis of the corpus callosum (ACC). Here, we report a novel 10.4 Mb interstitial deletion on 3q12.33q22.1 including GATA2 and ZNF148 in a child with developmental delay, agenesis of the corpus callosum, and vertebral segmentation defects. With this diagnosis, we were able to suggest preemptive referrals to hematology/oncology and allergy/immunology for close monitoring of early myelodysplasia. We also propose a possible link between ZNF148 loss of function variants and ACC., (© 2024 The Authors. American Journal of Medical Genetics Part A published by Wiley Periodicals LLC.)
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- 2024
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20. The Role of Antibody-Based Therapies in Neuro-Oncology.
- Author
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Ramapriyan, Rishab, Sun, Jing, Curry, Annabel, Richardson, Leland G., Ramesh, Tarun, Gaffey, Matthew A., Gedeon, Patrick C., Gerstner, Elizabeth R., Curry, William T., and Choi, Bryan D.
- Subjects
CHIMERIC antigen receptors ,IMMUNE checkpoint inhibitors ,ANTIBODY-drug conjugates ,MONOCLONAL antibodies ,DRUG target - Abstract
This review explores the evolving landscape of antibody-based therapies in neuro-oncology, in particular, immune checkpoint inhibitors and immunomodulatory antibodies. We discuss their mechanisms of action, blood-brain barrier (BBB) penetration, and experience in neuro-oncological conditions. Evidence from recent trials indicates that while these therapies can modulate the tumor immune microenvironment, their clinical benefits remain uncertain, largely due to challenges with BBB penetration and tumor-derived immunosuppression. This review also examines emerging targets such as TIGIT and LAG3, the potential of antibodies in modulating the myeloid compartment, and tumor-specific targets for monoclonal antibody therapy. We further delve into advanced strategies such as antibody–drug conjugates and bispecific T cell engagers. Lastly, we explore innovative techniques being investigated to enhance antibody delivery, including CAR T cell therapy. Despite current limitations, these therapies hold significant therapeutic potential for neuro-oncology. Future research should focus on optimizing antibody delivery to the CNS, identifying novel biological targets, and discovering combination therapies to address the hostile tumor microenvironment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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21. Neurological adverse effects of chimeric antigen receptor T-cell therapy.
- Author
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Saleki, Kiarash, Mohamadi, Mohamad Hosein, Alijanizadeh, Parsa, and Rezaei, Nima
- Subjects
CHIMERIC antigen receptors ,T cells ,CYTOKINE release syndrome ,CEREBRAL edema ,SYMPTOMS - Abstract
Chimeric antigen receptor (CAR) T-cell is among the most prevalent approaches that act by directing T-cells toward cancer; however, they need to be optimized to minimize side effects and maximize efficacy before being used as standard treatment for malignancies. Neurotoxicity associated with CAR T-cell therapy has been well-documented in recent works. In this regard, two established syndromes exist. Immune effector cell-associated neurotoxicity syndrome (ICANS), previously called cytokine release encephalopathy syndrome (CRES), is a neuropsychiatric condition which can occur after therapy by immune effector cells (IEC) and T-lymphocytes utilizing treatments. Another syndrome is cytokine release syndrome (CRS), which may overlap with ICANS. ICANS clinical manifestations include cerebral edema, mild lethargy, aphasia, and seizures. Notably, ICANS is associated with changes to EEG and neuroradiological findings. Therefore, it is necessary to make a timely and accurate diagnosis of neurological complications of CAR T-cells by clinical presentations, neuroimaging, and EEG. Since neurological events by different CAR T-cell products are heterogeneous, guides should be developed according to each product. Here, we provide an updated review of general information on CAR T-cell therapies and applications, neurological syndromes associated with their use, and risk factors contributing to ICANS. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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22. Relations between Video Game Engagement and Social Development in Children: The Mediating Role of Executive Function and Age-Related Moderation.
- Author
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Xu, Ke, Geng, Shuliang, Dou, Donghui, and Liu, Xiaocen
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EXECUTIVE function ,SOCIAL development ,CHILD development ,VIDEO games ,GAMES - Abstract
The global proliferation of video games, particularly among children, has led to growing concerns about the potential impact on children's social development. Executive function is a cognitive ability that plays a crucial role in children's social development, but a child's age constrains its development. To examine the association between video game engagement and children's social development while considering the mediating role of executive function and the moderating role of age, a questionnaire was distributed to a sample of 431 parents. The results revealed a negative relation between video game engagement and social development in children, with executive function found to mediate this relation fully. Additionally, the negative association between video game engagement and executive function became more pronounced as children grew older. In light of these findings, it is advisable to adopt proactive strategies to limit excessive video game use, consider the developmental characteristics of children at different ages, and prioritize the promotion of executive function to facilitate social development among children. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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23. Pathogenic variants in PIK3CA are associated with clinical phenotypes of kaposiform lymphangiomatosis, generalized lymphatic anomaly, and central conducting lymphatic anomaly.
- Author
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Grenier, Jeremy M., Borst, Alexandra J., Sheppard, Sarah E., Snyder, Kristen M., Li, Dong, Surrey, Lea F., Al‐Ibraheemi, Alyaa, Weber, David R., Treat, James R., Smith, Christopher L., Laje, Pablo, Dori, Yoav, Adams, Denise M., Acord, Michael, and Srinivasan, Abhay S.
- Published
- 2023
- Full Text
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24. Autoimmune Limbic Encephalitis in Patients with Hematologic Malignancies after Haploidentical Hematopoietic Stem Cell Transplantation with Post-Transplant Cyclophosphamide.
- Author
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Heo, Bu Yeon, Lee, Myung-Won, Choi, Suyoung, Jung, Yunju, Pham, Thi Thuy Duong, Jang, Yunseon, Park, Jung-Hyun, Kang, Sora, Koh, Jeong Suk, Jo, Deog-Yeon, Kwon, Jaeyul, and Song, Ik-Chan
- Subjects
HEMATOPOIETIC stem cell transplantation ,T cells ,METHYL aspartate receptors ,REGULATORY T cells ,HEMATOLOGIC malignancies ,T cell differentiation ,ENCEPHALITIS - Abstract
Autoimmune limbic encephalitis (LE) is a rare, but devastating complication of allogeneic hematopoietic stem cell transplantation (HSCT). There is currently limited evidence describing the risk factors, laboratory features, and underlying mechanisms of this neurologic adverse event. We retrospectively reviewed available clinical, imaging, and laboratory data from adult patients with hematological malignancies who underwent haploidentical HSCT with post-transplant cyclophosphamide (PTCy) at Chungnam National University Hospital from June 2016 to May 2020. Patients who developed LE were compared to those who did not based on clinical assessment, serum inflammatory biomarkers, and reconstitution of various T cell populations. Of 35 patients, 4 developed LE. There were no differences in patient demographics, donor demographics, or treatment conditions between patients that did and did not develop LE. Overall, patients with LE had worse clinical outcomes and overall survival than those without. In addition, they tended to have higher markers of systemic inflammation in the early post-transplant period, including fever, C-reactive protein (CRP), and cytokines. Remarkably, baseline interleukin-6 levels before HSCT were found to be higher in patients who developed LE than those who did not. In addition, analysis of T cell subsets showed impaired expansion of CD25+FOXP3+ regulatory T (Treg) cells in LE compared to non-LE patients despite appropriate reconstitution of the total CD4+ T cell population. Patients that developed LE within the first 30 days of HSCT were likely to have high serum IL-6 among other inflammatory cytokines coupled with suppression of regulatory T cell differentiation. Further work is needed on the mechanisms underlying impaired Treg expansion following HSCT and potential therapies. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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25. Early diagnosis of sinusoidal obstruction syndrome after hematopoietic stem cell transplantation, with modified diagnostic criteria including refractory thrombocytopenia.
- Author
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Ichikawa, Hiroya, Yakushijin, Kimikazu, Miyata, Yoshiharu, Kanehira, Hirofumi, Joyce, Miki, Hirakawa, Yuri, Matsumoto, Sakuya, Nagao, Shigeki, Sakai, Rina, Kurata, Keiji, Kitao, Akihito, Saito, Yasuyuki, Kawamoto, Shinichiro, Yamamoto, Katsuya, Ito, Mitsuhiro, Murayama, Tohru, Matsuoka, Hiroshi, and Minami, Hironobu
- Published
- 2023
- Full Text
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26. The role of MSCs and CAR-MSCs in cellular immunotherapy.
- Author
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Yan, Lun, Li, Jing, and Zhang, Cheng
- Subjects
CYTOKINE release syndrome ,MESENCHYMAL stem cells ,CHIMERIC antigen receptors ,IMMUNOTHERAPY ,DENDRITIC cells ,KILLER cells ,PROGRAMMED cell death 1 receptors - Abstract
Chimeric antigen receptors (CARs) are widely used by T cells (CAR-T cells), natural killer cells dendritic cells and macrophages, and they are of great importance in cellular immunotherapy. However, the use of CAR-related products faces several challenges, including the poor persistence of cells carrying CARs, cell dysfunction or exhaustion, relapse of disease, immune effector cell-associated neurotoxicity syndrome, cytokine release syndrome, low efficacy against solid tumors and immunosuppression by the tumor microenvironment. Another important cell therapy regimen involves mesenchymal stem cells (MSCs). Recent studies have shown that MSCs can improve the anticancer functions of CAR-related products. CAR-MSCs can overcome the flaws of cellular immunotherapy. Thus, MSCs can be used as a biological vehicle for CARs. In this review, we first discuss the characteristics and immunomodulatory functions of MSCs. Then, the role of MSCs as a source of exosomes, including the characteristics of MSC-derived exosomes and their immunomodulatory functions, is discussed. The role of MSCs in CAR-related products, CAR-related product-derived exosomes and the effect of MSCs on CAR-related products are reviewed. Finally, the use of MSCs as CAR vehicles is discussed. 4iUUHD81rmzA1EBQmMyRQx Video Abstract [ABSTRACT FROM AUTHOR]
- Published
- 2023
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27. Combination Therapies with Kinase Inhibitors for Acute Myeloid Leukemia Treatment.
- Author
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Shinichiro Takahashi
- Subjects
ACUTE myeloid leukemia ,KINASE inhibitors ,LITERATURE reviews ,CHECKPOINT kinase 1 ,CELL differentiation - Abstract
Targeting kinase activity is considered to be an attractive therapeutic strategy to overcome acute myeloid leukemia (AML) since aberrant activation of the kinase pathway plays a pivotal role in leukemogenesis through abnormal cell proliferation and differentiation block. Although clinical trials for kinase modulators as single agents remain scarce, combination therapies are an area of therapeutic interest. In this review, the author summarizes attractive kinase pathways for therapeutic targets and the combination strategies for these pathways. Specifically, the review focuses on combination therapies targeting the FLT3 pathways, as well as PI3K/AKT/mTOR, CDK and CHK1 pathways. From a literature review, combination therapies with the kinase inhibitors appear more promising than monotherapies with individual agents. Therefore, the development of efficient combination therapies with kinase inhibitors may result in effective therapeutic strategies for AML. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
28. Chimeric antigen receptor T‐cell therapy in multiple myeloma: A comprehensive review of current data and implications for clinical practice.
- Author
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Parikh, Rujul H. and Lonial, Sagar
- Subjects
CHIMERIC antigen receptors ,MULTIPLE myeloma ,T cells ,PLASMACYTOMA - Abstract
Multiple myeloma (MM) is a hematologic malignancy defined by the clonal proliferation of transformed plasma cells. Despite tremendous advances in the treatment paradigm of MM, a cure remains elusive for most patients. Although long‐term disease control can be achieved in a very large number of patients, the acquisition of tumor resistance leads to disease relapse, especially in patients with triple‐class refractory MM (defined as resistance to immunomodulatory agents, proteosome inhibitors, and monoclonal antibodies). There is an unmet need for effective treatment options in these patients. Chimeric antigen receptor (CAR) T‐cell therapy is a novel approach that has demonstrated promising efficacy in the treatment of relapsed, refractory MM (RRMM). These genetically modified cellular therapies have demonstrated deep and durable remissions in other B‐cell malignancies, and current efforts aim to achieve similar results in patients with RRMM. Early studies have demonstrated remarkable response rates with CAR T‐cell therapy in RRMM; however, durable responses with CAR T‐cell therapies in myeloma have yet to be realized. In this comprehensive review, the authors describe the development of CAR T‐cell therapies in myeloma, the outcomes of notable clinical trials, the toxicities and limitations of CAR T‐cell therapies, and the strategies to overcome therapeutic challenges of CAR T cells in the hope of achieving a cure for multiple myeloma. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
29. Low Plasma Levels of Hyaluronic Acid Might Rule Out Sinusoidal Obstruction Syndrome after Hematopoietic Stem Cell Transplantation.
- Author
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De Ramón Ortiz, Carmen, Justo Sanz, Raul, Beauverd, Yan, Humala, Karem, López de la Guia, Ana, De Paz, Raquel, Gasior, Mercedes, Gómez Prieto, Pilar, Fabra Urdiola, Marta, Canales Albendea, Miguel, Butta, Nora, and Jiménez Yuste, Victor
- Subjects
HEMATOPOIETIC stem cell transplantation ,HEPATIC veno-occlusive disease ,HYALURONIC acid ,STEM cell transplantation ,HEMATOPOIETIC stem cells ,CELL adhesion ,CORD blood - Abstract
Background. Sinusoidal obstructive syndrome (SOS) is a potentially fatal complication secondary to hematopoietic stem cell transplant (HSCT) conditioning. Endothelial damage plasma biomarkers such as plasminogen activator inhibitor-1 (PAI-1), hyaluronic acid (HA), and vascular adhesion molecule-1 (VCAM1) represent potential diagnostic tools for SOS. Methods. We prospectively collected serial citrated blood samples (baseline, day 0, day 7, and day 14) in all adult patients undergoing HSCT at La Paz Hospital, Madrid. Samples were later analyzed by ELISA (enzyme-linked immunosorbent assay) for HA, VCAM1, and PAI-1 concentrations. Results. During sixteen months, we prospectively recruited 47 patients. Seven patients (14%) were diagnosed with SOS according to the EBMT criteria for SOS/VOD diagnosis and received treatment with defibrotide. Our study showed a statistically significant elevation of HA on day 7 in SOS patients, preceding clinical SOS diagnosis, with a sensitivity of 100%. Furthermore, we observed a significant increase of HA and VCAM1 levels on day 14. Regarding risk factors, we observed a statistically significant association between SOS diagnosis and the fact that patients received 3 or more previous lines of treatment before HSCT. Conclusions. The early significant increase in HA levels observed opens the door to a noninvasive peripheral blood test which could have the potential to improve diagnosis and facilitate prophylactic and therapeutic management of SOS before clinical/histological damage is established. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
30. Kaposiform lymphangiomatosis: Diagnosis, pathogenesis, and treatment.
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McDaniel, C. Griffin, Adams, Denise M., Steele, Kimberley E., Hammill, Adrienne M., Merrow, A. Carl, Crane, Janet L., Smith, Christopher L., Kozakewich, Harry P. W., and Le Cras, Timothy D.
- Published
- 2023
- Full Text
- View/download PDF
31. Novel pathophysiological insights into CAR-T cell associated neurotoxicity.
- Author
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Genoud, Vassilis and Migliorini, Denis
- Subjects
CYTOKINE release syndrome ,NEUROTOXICOLOGY ,CHIMERIC antigen receptors ,T cells ,CELLULAR therapy - Abstract
Chimeric antigen receptor (CAR) T cell therapy represents a scientific breakthrough in the treatment of advanced hematological malignancies. It relies on cell engineering to direct the powerful cytotoxic T-cell activity toward tumor cells. Nevertheless, these highly powerful cell therapies can trigger substantial toxicities such as cytokine release syndrome (CRS) and immune cell-associated neurological syndrome (ICANS). These potentially fatal side effects are now better understood and managed in the clinic but still require intensive patient follow-up and management. Some specific mechanisms seem associated with the development of ICANS, such as cytokine surge caused by activated CAR-T cells, off-tumor targeting of CD19, and vascular leak. Therapeutic tools are being developed aiming at obtaining better control of toxicity. In this review, we focus on the current understanding of ICANS, novel findings, and current gaps. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
32. The Importance of New EBMT Criteria on the Diagnosis of Veno-Occlusive Liver Disease in Children.
- Author
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Füssiová, Mária, Švec, Peter, Horáková, Júlia, Sedláček, Petr, Rohoň, Peter, Celec, Peter, Boďová, Ivana, Adamčáková, Jaroslava, Sýkora, Tomáš, Dobšinská, Veronika, Pozdechová, Miroslava, Dóczyová, Dominika, Vargová, Santia, and Kolenová, Alexandra
- Subjects
LIVER disease diagnosis ,HEPATIC veno-occlusive disease ,HEMATOPOIETIC stem cell transplantation ,SYMPTOMS ,CHILD patients ,DIAGNOSIS - Abstract
Background: Early recognition and specific therapy facilitate a favorable disease course in hepatic venous-occlusive disease (HVOD) following hematopoietic stem cell transplantation (HCT). Diagnostic and classification criteria, published by the European Society for Blood and Marrow Transplantation (EBMT), better account for clinical differences in disease presentation in pediatric populations. Objectives: To compare the course of HVOD in children before and after the implementation of new EBMT criteria. Material and methods: The study retrospectively evaluates 26 HVODs in 179 children treated in a single HCT unit (Slovakia) comparing the period of 2014–2017 using the Baltimore and modified Seattle criteria with the period of 2018–2021, when new EBMT criteria were adopted. Results: No difference in HVOD incidence (11.2% vs. 14.8%, p = 0.46) and in time of diagnosis post-HCT (15.6 days vs. 15.7 days, p = 0.75) was found. With EBMT criteria we observed more frequent anicteric disease at diagnosis (50% vs. 87.5%, p = 0.04), lower serum bilirubin at diagnosis (3.4 mg/dL vs. 1.23 mg/dL, p = 0.045), and non-significant trends of shorter defibrotide treatment (21.7 days vs. 15.6 days, p = 0.73), decreased mortality (30% vs. 6.2%, p = 0.10) and shorter hospitalization (73.1 days vs. 59.6 days, p = 0.54). Conclusions: Different time periods around the implementation of new criteria are evaluated, underling that pediatric EBMT criteria for post-transplant HVOD diagnosis appear more sensitive. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
33. Letermovir use in children after hematopoietic cell transplantation: summary of reported data.
- Author
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Styczyński, Tomasz, Sadlok, Jagoda, Richert-Przygońska, Monika, and Czyżewski, Krzysztof
- Abstract
Introduction: Letermovir (LMV) is approved for primary prophylaxis of cytomegalovirus infection (CMVi) in CMV-seropositive adult patients undergoing allogeneic hematopoietic stem cell transplantation. However, it is not registered for CMVi preemptive treatment, CMVi secondary prophylaxis, or the treatment of CMV disease. There is very limited data regarding LMV's use in pediatric patients, as it has not been approved so far as any kind of treatment in children, with its use remaining off label. The aim of this study was to summarize reported data on the efficacy and safety of LMV in pediatric patients. Material and methods: Studies and case reports regarding LMV's use in pediatric patients were searched in PubMed. Results: Overall, nine reports that fulfilled the search criteria, published between 2019 and 2022, were found and analyzed. The total number of cases involved in research was 46 with patient age ranging from 2-19 years; one child was counted twice due to another transplant. The most common serostatus of donor/recipient was D+/R+ (47%), followed by D-/R+ (42%), then D+/R- (2%), and then unknown (9%). Most patients had received the transplant from a matched unrelated donor (40%). There were 47 incidents of LMV administration as CMV management strategy. The analyzed patients received LMV as primary prophylaxis (74%), secondary prophylaxis (15%), pre-emptive therapy (6%), or treatment of CMV disease (4%). One patient received LMV as a treatment and then as a secondary prophylaxis. In 44/46 (95.6%) cases, no symptomatic CMVi occurred during LMV administration, with only transient CMV DNA-emia present on rare occasions. Conclusion: The use of LMV is safe in pediatric patients. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
34. Angiotensin-Converting Enzyme (ACE) Insertion/Deletion (I/D) Polymorphism as a Conjoint Regulator of Coagulation, Fibrinolytic, and RAAS Pathway in Infertility and Associated Pregnancy Complications.
- Author
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Thakur, Sunil, Sharma, Vaishnavi, Kaur, Dipneet, and Purkait, Pulakes
- Subjects
PREGNANCY complications ,ANGIOTENSIN converting enzyme ,INFERTILITY ,RECURRENT miscarriage ,REPRODUCTIVE technology ,HUMAN artificial insemination - Abstract
Despite the increase in assisted reproductive technologies, the high rates of infertility and pregnancy complications are a major concern to infertility specialists worldwide. Infertility may be attributed to pregnancy complications like thrombophilia, preeclampsia and fibrin-induced recurrent pregnancy loss (RPL). Renin-angiotensin-aldosterone system (RAAS) directly or indirectly causes preeclampsia and thrombophilia through the fibrinolytic pathway that ultimately leads to RPL or infertility. The underlying mechanisms of this interaction are still unclear. The present comprehensive review is intended to demonstrate the role and interaction of RAAS and fibrinolytic pathways in pregnancy complications. How this interaction can induce pregnancy complications, and ultimately infertility, is also discussed in the light of current evidence. This study also presents common markers that link RAAS and fibrinolytic processes in developing thrombophilia, preeclampsia and RPL. The common link in these pathways is ACE gene I/D polymorphism. Apart from ACE, PAI-1, VIIa, XIIa, AT1R, AT1AA, and TF are common molecules that can delineate the underlying causes of pregnancy complications and infertility. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
35. Mechanisms of immune effector cell‐associated neurotoxicity syndrome after CAR‐T treatment.
- Author
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Gu, Tianning, Hu, Kejia, Si, Xiaohui, Hu, Yongxian, and Huang, He
- Published
- 2022
- Full Text
- View/download PDF
36. intrinsic and microenvironmental features of diffuse midline glioma: Implications for the development of effective immunotherapeutic treatment strategies.
- Author
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Persson, Mika L, Douglas, Alicia M, Alvaro, Frank, Faridi, Pouya, Larsen, Martin R, Alonso, Marta M, Vitanza, Nicholas A, and Dun, Matthew D
- Published
- 2022
- Full Text
- View/download PDF
37. Immunogenic Cell Death Enhances Immunotherapy of Diffuse Intrinsic Pontine Glioma: From Preclinical to Clinical Studies.
- Author
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Liu, Guohao, Qiu, Yanmei, Zhang, Po, Chen, Zirong, Chen, Sui, Huang, Weida, Wang, Baofeng, Yu, Xingjiang, and Guo, Dongsheng
- Subjects
CELL death ,GLIOMAS ,IMMUNOTHERAPY ,CENTRAL nervous system ,CANCER chemotherapy - Abstract
Diffuse intrinsic pontine glioma (DIPG) is the most lethal tumor involving the pediatric central nervous system. The median survival of children that are diagnosed with DIPG is only 9 to 11 months. More than 200 clinical trials have failed to increase the survival outcomes using conventional cytotoxic or myeloablative chemotherapy. Immunotherapy presents exciting therapeutic opportunities against DIPG that is characterized by unique and heterogeneous features. However, the non-inflammatory DIPG microenvironment greatly limits the role of immunotherapy in DIPG. Encouragingly, the induction of immunogenic cell death, accompanied by the release of damage-associated molecular patterns (DAMPs) shows satisfactory efficacy of immune stimulation and antitumor strategies. This review dwells on the dilemma and advances in immunotherapy for DIPG, and the potential efficacy of immunogenic cell death (ICD) in the immunotherapy of DIPG. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
38. The Evolution of Chimeric Antigen Receptor T-Cell Therapy in Children, Adolescents and Young Adults with Acute Lymphoblastic Leukemia.
- Author
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Ragoonanan, Dristhi, Sheikh, Irtiza N., Gupta, Sumit, Khazal, Sajad J., Tewari, Priti, Petropoulos, Demetrios, Li, Shulin, and Mahadeo, Kris M.
- Subjects
CHIMERIC antigen receptors ,YOUNG adults ,LYMPHOBLASTIC leukemia ,ACUTE leukemia ,T cells - Abstract
Chimeric antigen receptor T-cell (CAR T) therapy is a revolutionary treatment for pediatric, adolescent and young adult patients (AYA) with relapsed/refractory B-cell acute lymphoblastic leukemia. While the landscape of immunotherapy continues to rapidly evolve, widespread use of CAR T therapy is limited and many questions remain regarding the durability of CAR T therapy, methods to avoid CAR T therapy resistance and the role of consolidative stem cell transplant. Modified strategies to develop effective and persistent CAR T cells at lower costs and decreased toxicities are warranted. In this review we present current indications, limitations and future directions of CAR T therapy for ALL in the pediatric and AYA population. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
39. Severe persistent neurotoxicity associated with CAR T therapy in children.
- Author
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Andrew EC, Hughes D, Gilsenan M, Mignone C, Khaw SL, and Wang SS
- Subjects
- Humans, Child, Cytokine Release Syndrome, Immunotherapy, Adoptive adverse effects, Adaptor Proteins, Signal Transducing, Antigens, CD19 adverse effects, Receptors, Chimeric Antigen, Neurotoxicity Syndromes etiology
- Abstract
CD19-directed chimeric antigen receptor (CAR) T-cell therapy is an important therapy for relapsed or refractory acute lymphoblastic leukaemia, but its use carries the risk of immune effector cell-associated neurotoxicity syndrome (ICANS). In children, severe ICANS is almost universally reported in association with cytokine release syndrome and is reversible. We describe two cases of severe, intractable neurotoxicity following CAR T-cell therapy in children with pre-existing central nervous system (CNS) vulnerabilities. The cases were atypical in their delayed onset and independence from cytokine release syndrome and did not respond to standard therapies., (© 2023 British Society for Haematology and John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
40. Review: Sustainable Clinical Development of CAR-T Cells – Switching From Viral Transduction Towards CRISPR-Cas Gene Editing.
- Author
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Wagner, Dimitrios L., Koehl, Ulrike, Chmielewski, Markus, Scheid, Christoph, and Stripecke, Renata
- Subjects
GENOME editing ,CRISPRS ,BIOENGINEERING ,SUSTAINABLE development ,MANUFACTURING cells - Abstract
T cells modified for expression of Chimeric Antigen Receptors (CARs) were the first gene-modified cell products approved for use in cancer immunotherapy. CAR-T cells engineered with gammaretroviral or lentiviral vectors (RVs/LVs) targeting B-cell lymphomas and leukemias have shown excellent clinical efficacy and no malignant transformation due to insertional mutagenesis to date. Large-scale production of RVs/LVs under good-manufacturing practices for CAR-T cell manufacturing has soared in recent years. However, manufacturing of RVs/LVs remains complex and costly, representing a logistical bottleneck for CAR-T cell production. Emerging gene-editing technologies are fostering a new paradigm in synthetic biology for the engineering and production of CAR-T cells. Firstly, the generation of the modular reagents utilized for gene editing with the CRISPR-Cas systems can be scaled-up with high precision under good manufacturing practices, are interchangeable and can be more sustainable in the long-run through the lower material costs. Secondly, gene editing exploits the precise insertion of CARs into defined genomic loci and allows combinatorial gene knock-ins and knock-outs with exciting and dynamic perspectives for T cell engineering to improve their therapeutic efficacy. Thirdly, allogeneic edited CAR-effector cells could eventually become available as "off-the-shelf" products. This review addresses important points to consider regarding the status quo , pending needs and perspectives for the forthright evolution from the viral towards gene editing developments for CAR-T cells. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
41. Computational Analysis of the Related Factors of Deep Vein Thrombosis (DVT) Formation in Patients Undergoing Hip Fracture Surgery.
- Author
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Zhou, Yi, Chen, Huali, Zhang, Yan, Yang, Chao, Yi, Xiaohui, Liu, Shanshan, and Zeng, Yao
- Subjects
SURGICAL complication risk factors ,CORONARY heart disease risk factors ,HYPERTENSION risk factors ,HIP surgery ,RESEARCH ,AGE distribution ,RETROSPECTIVE studies ,ACQUISITION of data ,TREATMENT duration ,CARDIOVASCULAR diseases ,VENOUS thrombosis ,RISK assessment ,T-test (Statistics) ,SEX distribution ,HYPERLIPIDEMIA ,MEDICAL records ,STATISTICAL correlation ,FIBRIN fibrinogen degradation products ,DISEASE risk factors ,DISEASE complications - Abstract
A retrospective study was conducted on 51 patients undergoing hip fracture surgery to investigate the factors associated with the formation of deep venous thrombosis (DVT). The independent sample t-test and correlation analysis were used to sort out and analyze the data. The findings are as follows. (1) Different gender samples showed significant differences in the Caprini score and thrombus location. Most DVTs in females are located in the posterior tibial vein and intermuscular veins. The Caprini score of females was significantly higher than that of males. (2) Age displays a positive correlation with DVT, coronary heart disease, hypertension, and different surgical types, respectively. (3) There is a correlation between age and operation duration. (4) Hyperlipidemia and cerebrovascular disease show a positive correlation with DVT. (5) There was a significant negative correlation between the Caprini score and the quantification of D-dimer. This indicates that in this sample, the higher the patients' Caprini score is, the lower the quantitation of D-dimer will be. (6) Hyperlipidemia and cardiac insufficiency show a positive correlation with cerebrovascular disease. Patients with hyperlipidemia and cardiac insufficiency may also suffer from cerebrovascular diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
42. CAR-T Cells/-NK Cells in Cancer Immunotherapy and the Potential of MSC to Enhance Its Efficacy: A Review.
- Author
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Chan, Ler Yie, Dass, Sylvia Annabel, Tye, Gee Jun, Imran, Siti A. M., Wan Kamarul Zaman, Wan Safwani, and Nordin, Fazlina
- Subjects
CANCER cells ,MESENCHYMAL stem cells ,CHIMERIC antigen receptors ,CANCER treatment ,IMMUNOTHERAPY - Abstract
The chimeric antigen receptor (CAR) plays a dynamic role in targeting tumour-associated antigens in cancer cells. This novel therapeutic discovery combines fragments of monoclonal antibodies with the signalling and co-stimulatory domains that have been modified to its current fourth generation. CAR has been widely implemented in T-cells and natural killer (NK) cells immunotherapy. The significant advancement in CAR technology is evident based on numerous ongoing clinical trials on CAR-T/-NK cells and successful CAR-related products such as Kymriah (Novartis) and Yescarta (Kite Pharma, Gilead). Another important cell-based therapy is the engineering of mesenchymal stem cells (MSC). Researchers have been exploring MSCs and their innate homing abilities to tumour sites and secretion cytokines that bridge both CAR and MSC technologies as a therapeutic agent. This combination allows for both therapies to overcome each one's flaw as an immunotherapy intervention. Herein, we have provided a concise review on the background of CAR and its applications in different cancers, as well as MSCs' unique ability as delivery vectors for cancer therapy and the possibility of enhancing the CAR-immune cells' activity. Hence, we have highlighted throughout this review the synergistic effects of both interventions. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
43. Cytokine Release Syndrome and Associated Acute Toxicities in Pediatric Patients Undergoing Immune Effector Cell Therapy or Hematopoietic Cell Transplantation.
- Author
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Baumeister, Susanne H. C., Mohan, Gopi S., Elhaddad, Alaa, and Lehmann, Leslie
- Subjects
HEMATOPOIETIC stem cell transplantation ,CYTOKINE release syndrome ,CELLULAR therapy ,CHILD patients ,PEDIATRIC therapy ,T cells - Abstract
Immune effector cells (IEC) are a powerful and increasingly targeted tool, particularly for the control and eradication of malignant diseases. However, the infusion, expansion, and persistence of autologous or allogeneic IEC or engagement of endogenous immune cells can be associated with significant systemic multi-organ toxicities. Here we review the signs and symptoms, grading and pathophysiology of immune-related toxicities arising in the context of pediatric immunotherapies and haploidentical T cell replete Hematopoietic Cell Transplantation (HCT). Principles of management are discussed with particular focus on the intersection of these toxicities with the requirement for pediatric critical care level support. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
44. Genetic Therapy and Molecular Targeted Therapy in Oncology: Safety, Pharmacovigilance, and Perspectives for Research and Clinical Practice.
- Author
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Orzetti, Sabrina, Tommasi, Federica, Bertola, Antonella, Bortolin, Giorgia, Caccin, Elisabetta, Cecco, Sara, Ferrarin, Emanuela, Giacomin, Elisa, and Baldo, Paolo
- Subjects
DRUG side effects ,DRUG therapy ,DRUG target ,ONCOLOGY ,GENE therapy ,SAFETY - Abstract
The impressive advances in the knowledge of biomarkers and molecular targets has enabled significant progress in drug therapy for crucial diseases such as cancer. Specific areas of pharmacology have contributed to these therapeutic outcomes—mainly targeted therapy, immunomodulatory therapy, and gene therapy. This review focuses on the pharmacological profiles of these therapeutic classes and intends, on the one hand, to provide a systematic definition and, on the other, to highlight some aspects related to pharmacovigilance, namely the monitoring of safety and the identification of potential toxicities and adverse drug reactions. Although clinicians often consider pharmacovigilance a non-priority area, it highlights the risk/benefit ratio, an essential factor, especially for these advanced therapies, which represent the most innovative and promising horizon in oncology. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
45. L-arginine Improves Plasma Lipid Profile and Muscle Inflammatory Response in Trained Rats After High-Intense Exercise.
- Author
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Pinto da Silva Jr., Edenilson, Borges, Leandro, Lacerda Bachi, André Luis, Massao Hirabara, Sandro, and Herling Lambertucci, Rafael
- Subjects
ARGININE ,BLOOD lipids ,MYOSITIS ,EXERCISE intensity ,HIGH-intensity interval training - Abstract
Purpose: This study aimed to evaluate whether supplementation with L-arginine alone or in combination with physical exercise training can modulate rats' lipid and inflammatory profiles after a single intense exercise session. Methods: Male Wistar rats were divided into four different groups: control (C), trained (T), supplemented with L-arginine (C + A) and trained and supplemented (T + A). Animals from supplemented groups (C + A and T + A groups) received 300 mg/kg animal body weight L-arginine diluted in 30 mL of drinking water for 8 weeks. Exercise training protocol (moderate intensity--70% achieved in the maximum effort test) was held 5 days/week for 8 weeks. Results: Exercise training induced a decrease in the amount of plasma, cholesterol and triglyceride totals, and skeletal muscle VEGF and CINC. Supplementation alone showed a benefit by reducing LDL levels. Conclusion: Training combined with supplementation showed a pronounced reduction in skeletal muscle VEGF and CINC amount. L-arginine supplementation, especially when associated with the regular aerobic physical exercise at moderate intensity was able to improve not only plasma lipid profile but also the inflammatory response of skeletal muscle immediately after an exhaustive physical exercise session. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
46. CHEMICAL STABILITY IN ARTIFICIAL SALIVA OF ZIRCONIA BIOCERAMICS.
- Author
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VOLCEANOV, ENIKO, POPA, CRISTINA GEORGIANA, VOLCEANOV, ADRIAN, and CIUCĂ, SORIN
- Subjects
ARTIFICIAL saliva ,CHEMICAL stability ,BIOCERAMICS ,ZIRCONIUM oxide ,DENTAL materials ,SCANNING electron microscopy ,CEMENT admixtures - Abstract
Copyright of Romanian Journal of Materials / Revista Romana de Materiale is the property of Foundation for Materials Science & Engineering Serban Solacolu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
47. Author Index.
- Subjects
- AGARWAL, P., BALYAN, R., BOUCHARD, P.
- Published
- 2019
- Full Text
- View/download PDF
48. Defibrotide-treated patients with anicteric or icteric veno-occlusive disease/sinusoidal obstruction syndrome after hematopoietic cell transplantation: an EBMT study.
- Author
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Mohty M, Cluzeau T, Jubert C, Lawson S, Ryan RJ, Hanvesakul R, and Perruccio K
- Subjects
- Humans, Polydeoxyribonucleotides therapeutic use, Hematopoietic Stem Cell Transplantation adverse effects, Hepatic Veno-Occlusive Disease etiology, Vascular Diseases
- Published
- 2022
- Full Text
- View/download PDF
49. Diagnosis, grading and management of toxicities from immunotherapies in children, adolescents and young adults with cancer.
- Author
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Ragoonanan D, Khazal SJ, Abdel-Azim H, McCall D, Cuglievan B, Tambaro FP, Ahmad AH, Rowan CM, Gutierrez C, Schadler K, Li S, Di Nardo M, Chi L, Gulbis AM, Shoberu B, Mireles ME, McArthur J, Kapoor N, Miller J, Fitzgerald JC, Tewari P, Petropoulos D, Gill JB, Duncan CN, Lehmann LE, Hingorani S, Angelo JR, Swinford RD, Steiner ME, Hernandez Tejada FN, Martin PL, Auletta J, Choi SW, Bajwa R, Dailey Garnes N, Kebriaei P, Rezvani K, Wierda WG, Neelapu SS, Shpall EJ, Corbacioglu S, and Mahadeo KM
- Subjects
- Adolescent, Adult, Age Factors, Age of Onset, Antineoplastic Agents, Immunological adverse effects, Child, Hematopoietic Stem Cell Transplantation adverse effects, Hematopoietic Stem Cell Transplantation methods, Humans, Immunologic Factors adverse effects, Immunotherapy methods, Neoplasms diagnosis, Neoplasms epidemiology, Neoplasms pathology, Receptors, Chimeric Antigen immunology, Receptors, Chimeric Antigen metabolism, Severity of Illness Index, Transfusion-Related Acute Lung Injury diagnosis, Transfusion-Related Acute Lung Injury etiology, Transfusion-Related Acute Lung Injury therapy, Young Adult, Drug-Related Side Effects and Adverse Reactions diagnosis, Drug-Related Side Effects and Adverse Reactions pathology, Drug-Related Side Effects and Adverse Reactions therapy, Immunotherapy adverse effects, Neoplasms therapy, Transfusion Reaction diagnosis, Transfusion Reaction pathology, Transfusion Reaction therapy
- Abstract
Cancer immunotherapies are associated with remarkable therapeutic response rates but also with unique and severe toxicities, which potentially result in rapid deterioration in health. The number of clinical applications for novel immune effector-cell therapies, including chimeric antigen receptor (CAR)-expressing cells, and other immunotherapies, such as immune-checkpoint inhibitors, is increasing. In this Consensus Statement, members of the Pediatric Acute Lung Injury and Sepsis Investigators (PALISI) Network Hematopoietic Cell Transplantation-Cancer Immunotherapy (HCT-CI) Subgroup, Paediatric Diseases Working Party (PDWP) of the European Society of Blood and Marrow Transplantation (EBMT), Supportive Care Committee of the Pediatric Transplantation and Cellular Therapy Consortium (PTCTC) and MD Anderson Cancer Center CAR T Cell Therapy-Associated Toxicity (CARTOX) Program collaborated to provide updated comprehensive recommendations for the care of children, adolescents and young adults receiving cancer immunotherapies. With these recommendations, we address emerging toxicity mitigation strategies, we advocate for the characterization of baseline organ function according to age and discipline-specific criteria, we recommend early critical care assessment when indicated, with consideration of reversibility of underlying pathology (instead of organ failure scores) to guide critical care interventions, and we call for researchers, regulatory agencies and sponsors to support and facilitate early inclusion of young patients with cancer in well-designed clinical trials.
- Published
- 2021
- Full Text
- View/download PDF
50. The coming of age of the pediatric EBMT criteria.
- Author
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Corbacioglu S
- Subjects
- Child, Humans, Transplantation, Homologous, Hematopoietic Stem Cell Transplantation
- Published
- 2021
- Full Text
- View/download PDF
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