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1. Tissue Distribution of Human α1-Microglobulin

Author

Toshio Shikata, Kimiteru Takagi, Toshihiko Shimoda, Kohjin Kin, Yoshihisa Itoh, Tadashi Kawai, and Tadashi Kasahara

Subjects
Immunodiffusion, Chemical Phenomena, Lymphocyte, Carbohydrates, Fluorescent Antibody Technique, Biology, Alpha-Globulins, medicine, Humans, Lymphocytes, Amino Acids, Immunoelectrophoresis, Glycoproteins, Gel electrophoresis, Beta-2 microglobulin, Articles, General Medicine, Precipitin, Ouchterlony double immunodiffusion, Molecular biology, Staining, Molecular Weight, Chemistry, medicine.anatomical_structure, Tubular proteinuria
Abstract

Human alpha(1)-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. The carbohydrate content was 21.7%. Anti-alpha(1)-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified alpha(1)-microglobulin, as well as to normal human serum and urine. Sera from the domestic chicken, mouse, rat, rabbit, dog, calf, cow, goat, sheep, and horse, however, did not react to anti-alpha(1)-microglobulin serum in immunodiffusion. The lymphocyte culture supernate was found to contain alpha(1)-microglobulin. Both thymus-derived(T)- and bone marrow-derived(B)-lymphocyte culture media clearly displayed a specific precipitin line against anti-alpha(1)-microglobulin serum when tested with the Ouchterlony immunodiffusion method. The tissue distribution of alpha(1)-microglobulin was studied under immunofluorescence, and a positive staining was recognized on the lymphocyte surface. Identical staining patterns were noted on both T and B lymphocytes, though B lymphocytes took a more intense stain. It would thus seem quite possible that lymphocytes are the primary source of alpha(1)-microglobulin and that this is filtered through the glomerular basement membrane and partly reabsorbed by the renal tubules. This, then, would suggest the possibility that alpha(1)-microglobulin shares some immunological role in vivo with lymphocytes and(or) is one of the membrane proteins of lymphocytes.

Published
1979
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2. Therapeutic Evaluation on Combination Therapy with IG-100, pH4-Treated Human Immunoglobulin Preparation for i.v. Administration, and Antibiotics Against Severe Infections

Author

Keizo MATSUMOTO, Toru MASAOKA, Toru NAKAMURA, Kaoru SHIMADA, Ichiro URUSHIZAKI, Yutaka KOGO, Akira SAITO, Masumi TOMIZAWA, Ichiro NAKAYANA, Chikara MIKUNI, Kazuo TAKEBE, Katsuhiro OKAMOTO, Shuichiro YOSHIDA, Katsutoshi KARIYA, Yoko TOYAMA, Tomio ONUMA, Teruo NAKAMURA, Takeshi OSONOI, Shigeru OCHIAI, Morio SAGARA, Shigetoshi YANAGIYA, Osamu UEHARA, Naoyoshi MASAKI, Toyokazu TAMURA, Masao TAMURA, Takashi ITO, Yoshikatsu NEMOTO, Kiyoshi KONNO, Kotaro OIZUMI, Akira WATANABE, Shigeo KARIYONE, Tatsumi UCHIKA, Shin MATSUDA, Tadashi MAEKAWA, Shogo SHINONOME, Yasusada MIURA, Shinobu SAKAMOTO, Akihiro TSUBOYAMA, Shiro MIWA, Tadashi MIYAHARA, Jingoro SHIMADA, Hiroichi TANIMOTO, Yoshitaka NAKAMORI, Naohiko CHONABAYASHI, Hideyo KATSUNUMA, Yoshinori MIYAMOTO, Junzaburo KABE, Yasufumi MIYAMOTO, Kazuko OKABE, Keimei MASHIMO, Sumio YAMAOKA, Tetsuro UKAI, Katsunori UENO, Keiichi NAKAGAWA, Kohjin KIN, Fukuo IIJIMA, Takeshi ABE, Tadatoshi KINOSHITA, Jyuzo MATSUDA, Kazuo KAWASUGI, Toshiro OYAMA, Ichita AMAKI, Toshiteru OSHIMA, Hiroshi TAKEO, Hiroyuki KOBAYASHI, Hiroaki TAKEDA, Shin KAWAI, Hideo TERADA, Shoji HAGIWARA, Haruo NIIKURA, Kazuhiko MATSUNO, Hiraku MORI, Kunikane KIN, Yoshio TAKIZAWA, Ippei FUJIMORI, Yoshio KOBAYASHI, Akio ONAKA, Hiroshi NAKAMURA, Akira SHIBATA, Yoshiaki MORIYAMA, Hajime TAKEDA, Osamu SEKINE, Kaoru OYAMA, Michiko KANAKI, Kenichi HATTORI, Takashi YOSHIDA, Toshihiko TAKEUCHI, Masahito KATO, Toshiyuki YAMAMOTO, Kanzo SUZUKI, Hidekazu HANAKI, Motoko YAMAMOTO, Jiro YURA, Nagao SHINAGAWA, Tetsuro TAKAOKA, Akishige NAKAMURA, Yutaka HIROTA, Yoshihisa FUJIWARA, Masataka SASADA, Akemi KUBO, Tsuyoshi YONEZAWA, Yoshio KANAYAMA, Hirotoshi SHIBATA, Katsuji SAKAI, Takami UEDA, Teruo KITANI, Ichiro KONISHI, Kojiro YASUNAGA, Yuruko OKAMOTO, Keigo MAEHARA, Yoshihiro UEDA, Atsushi HORIUCHI, Hirofumi HASEGAWA, Haruto UCHINO, Hiroyoshi SAWADA, Shiro NAKAYAMA, Kenichi NAGAI, Kiyoyasu NAGAI, Akihisa KANAMURA, Soichiro YOSHIYASU, Takao HATTORI, Minoru NIIMOTO, Yukihisa MIYOSHI, Sadanori HAMAMOTO, Minoru TANADA, Hideyuki NAKANE, Rinzo SOEJIMA, Yoshito NIKI, Chikara NAKAHAMA, Toshio KANEKO, Kenji SHINOHARA, Kohei KAKU, Eiro TSUBURA, Masakazu TAMURA, Tomohiro KUSABA, Yoshiyuki NIHO, Seiichi OKAMURA, Masahide TAKII, Michio ONO, Kohei HARA, Atsushi SAITO, Keizo YAMAGUCHI, Yoji SUZUYAMA, Yoshiteru SHIGENO, Hiroshi TOMITA, Masao NAGASAWA, Michito ICHIMARU, Shuichi IKEDA, Tomoyuki HARADA, Tsuyoshi NAGATAKE, Shukuro ARAKI, Masayuki Ando, Mineharu SUGIMOTO, and Moritaka SUGA

Subjects
I v administration, Combination therapy, medicine.drug_class, business.industry, Clinical investigation, Antibiotics, Immunology, medicine, General Medicine, Therapeutic evaluation, business, Human immunoglobulin
Published
1984
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3. Cellular Cooperation in Lymphocyte Activation

Author

Yoshihisa Itoh, Kohjin Kin, Tadashi Kasahara, Tadashi Kawai, Mamoru Morita, and Kohei Shioiri-Nakano

Subjects
Cell type, T cell, B-cell receptor, Immunology, Biology, medicine.disease_cause, Interleukin 21, medicine, Cytotoxic T cell, Immunology and Allergy, IL-2 receptor, Antigen-presenting cell, Interleukin 3, CD40, Pokeweed mitogen, ZAP70, General Medicine, Molecular biology, Peripheral blood, medicine.anatomical_structure, Biochemistry, Concanavalin A, Staphylococcus aureus, Lymphocyte activation, biology.protein, Protein A
Abstract

Human T and B lymphocytes were highly purified from tonsil and peripheral blood, and their proliferative responses to various mitogens were studied. T cells [T(III)] were purified by filtration through a nylon fiber column (NC), followed by a double cycle of E rosette formation and Ficoll-Paque sedimentation. B cells [B(VIII)] were obtained from the NC-retained fraction by twice removal of E rosette forming cells. A second T cell preparation [T(VI)] was obtained from the NC-retained fraction. Purified T(III) and B(VIII) preparations contained less than 1% of other cell types. Phytohemagglutinin, pokeweed mitogen and concanavalin A (T mitogens) stimulated T(III) and T(VI) well, but had no effect upon B(VIII). Staphylococcus aureus Cowan I (SpA) and insolubilized protein A stimulated B(VIII) in the complete absence of T cells. Under the reconstituted culture of T(III) and B(VIII), responses to T mitogens were greatly enhanced by the addition of B(VIII). This enhancing effect was mainly caused by B cells and was not diminished by mitomycin treatment, suggesting that the observed T-B cooperation was attributable to direct cell-to-cell interaction. B(VIII) were also significantly stimulated by T mitogens in the presence of smallest number of T cells. SpA stimulated B(VIII) in proportion of their cell number independent of the addition of T cells. On the other hand, T(VI), which contained 3–10% B cells, showed rather higher response to T mitogens than T(III), failed to show coorepation with B(VIII).

Published
1979
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4. Contents, Vol. 59, 1979

Author

T.R. Deline, Yasuhiko Kano, S.O. Emejuaiwe, M. Tinelli, P. Eyre, Satoru Oka, Tetsuo Shimada, Christopher J. Meade, Takeru Ishikawa, Bernard J. De Cueninck, Rokuro Okuda, Hidenori Suzuki, D. Mancino, Elizabeth J. Harling, Takami Yoshida, Tadashi Kawai, Yoshihisa Itoh, B.F. Jones, H.O. Hanson, E. Penner, S.Y. Li Hsü, Keisuke Shinomiya, P. Falagiani, S.D. Jayakar, Haruki Mikawa, H.F. Hsü, Takao Hirao, Toshihiko Jyo, M.R. Robertson, J.W. Quin, Kunio Haga, Yoshinobu Yamamoto, N. Bevilacqua, Kohei Shioiri-Nakano, Herbert Megel, R.H. Holloway, Moriyasu Tsuji, Tiuzi Sindo, Nobuko Kessoku, H.J. Schiller, A. Peracino, Masayoshi Kiyoi, H.F. Cheng, R. Revoltella, Kyosuke Ozawa, G.D. Penick, T.J. Sharpe, A.G. Siccardi, J.A. Charlesworth, Tadashi Kasahara, J.C. Desmarais, M. Scaglia, F. Milgrom, Kohjin Kin, Donald L. Denney, Yuzuru Hayashi, and H. Smith

Subjects
business.industry, Immunology, Immunology and Allergy, Medicine, General Medicine, business
Published
1979
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5. Human IgA1 half-molecules: clinical and immunologic features in a patient with multiple myeloma

Author

Tadashi Kawai, Kohjin Kin, and Ikunosuke Sakurabayashi

Subjects
Immunology, Molecular Conformation, Fluorescent Antibody Technique, Immunoglobulin light chain, medicine.disease_cause, Biochemistry, chemistry.chemical_compound, medicine, Molecule, Humans, Immunoelectrophoresis, Multiple myeloma, Aged, Mutation, Chemistry, Immune Sera, Cell Biology, Hematology, Electrophoresis, Cellulose Acetate, IgA myeloma, medicine.disease, Molecular biology, Immunoglobulin A, Molecular Weight, Urea, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Female, Multiple Myeloma, Alpha chain, Enzymatic degradation
Abstract

Abnormal IgA1 half-molecules consisting of one heavy and one light chain were found in a patient (N.N.) with typical multiple myeloma. The serum and the urine of this patient contained both 7.0S and 3.9S IgA myeloma proteins. The IgA half-molecules (3.9S) were found to have a molecular weight of 59,000 daltons and were composed of one alpha1 chain of about 40,000 daltons and one light chain of 22,000 daltons. Furthermore, enzymatic degradation suggested that the alpha chain of the N.N. half-molecules had a large deletion in its Fc portion. We suggest that its heavy and light chains were probably bound noncovalently, since the interchains connecting the heavy and light chains of these IgA half-molecules were easily dissociated with 1% SDS and 8 M urea. Cytologic studies identified at least two types of myeloma cells, and it is possible that half-molecule IgA production might result from mutation among the myeloma cells producing whole- molecule IgA.

Published
1979

6. [A double-blind comparative study of aspoxicillin and piperacillin in the treatment of respiratory tract infections]

Author

Akira SAITO, Kiyofumi ISHIKAWA, Ichiro NAKAYAMA, Masumi TOMIZAWA, Morikuni ABE, Hidetoshi SHIBAKI, Takehisa CHIMOTO, Masahiro TANAKA, Choei ITO, Keisuke TAKEUCHI, Aiji KOJIMA, Kazuo SHIMOYAMA, Yoshinobu TAKASHIRO, Shinji OHNISHI, Masahide SHINOHARA, Akira UJIIE, Osamu YAJIMA, Fumio NAGAHAMA, Hisaji NISHIHARA, Yuichi SASAKI, Takehito NAKABAYASHI, Tetsuji KOROKU, Rensuke KURODA, Jutaro SHIMOMURA, Shin-ichi OSHIMA, Sokichi ONODERA, Nobuhiro SASAKI, Akira SUZUKI, Tohru NATSUIZAKA, Yohmei HIRAGA, Kohki KIKUCHI, Asako YAMAMOTO, Tsuyoshi KIKUIRI, Tadashi MURAKAMI, Kazufumi TSUCHIHASHI, Kazuo TAKEBE, Katsuhiro OKAMOTO, Shuichiro TOSHIDA, Tadanao OHMI, Shuichi TAKAHASHI, Katsutoshi KARIYA, Tomio ONUMA, Shigeru OCHIAI, Yoshihiro KUMASAKA, Shigetoshi YANAGIYA, Osamu UEHARA, Yasukimi WATABIKI, Naoyoshi MASAKI, Toyoichi TAMURA, Naoki TAMAZAWA, Hajime INOOKA, Masao TAMURA, Takashi ITOH, Ken-ichi TAKEUCHI, Ryoji OGATA, Jun KOMURO, Hiroaki ITOH, Tamotsu TAKISHIMA, Yasuo TANNO, Kiyoshi KONNO, Kotaro OHIZUMI, Akira WATANABE, Makoto KASHIWAGI, Hideo TOGASHI, Hidetaka SATO, Masato HAYASHI, Tsugushi ITOH, Izumi HAYASHI, Osamu SEKINE, Yoshimaru USUDA, Nobuki AOKI, Fusanosuke YAMASAKU, Yasutoshi SUZUKI, Masataka KATSU, Masanori ADACHI, Keimei MASHIMO, Tetsuro UKAI, Chizuru ITOH, Atsushi SAITO, Tadashi MIYAHARA, Hiroichi TANIMOTO, Akio TACHIBANA, Yoshitaka NAKAMORI, Naohiko CHONABAYASHI, Kunihiko YOSHIMURA, Junzaburo KABE, Hiroyoshi ISHIBASHI, Hideo IKEMOTO, Kazuyoshi WATANABE, Keiichi NAKAGAWA, Masaru KOYAMA, Kohjin KIN, Masami UEDA, Fukuo IIJIMA, Ryuji AKIYOSHI, Yuzo FUNATSU, Fuyuhiko HIGASHI, Hiroyuki KOBAYASHI, Hiroshi OSHITANI, Ippei FUJIMORI, Yoshio KOBAYASHI, Yasushi NAKAMURA, Kazufuto FUKAYA, Kenzo TAKAGI, Ikuo YAMAKAWA, Michihiro HARA, Tatsuo SATAKE, Toshihiko TAKEUCHI, Masahito KATO, Saburo KITAURA, Hidekazu HANAKI, Toshiyuki YAMAMOTO, Kanzo SUZUKI, Akihiko KISHIMOTO, Youichi ABE, Katsutaka TORIGAI, Kaoru OHYAMA, Kuninori SUZUKI, Nobuo OHYA, Mitsuru HAYASE, Nobuo MAEKAWA, Michiyasu NAKANISHI, Kohei AOKI, Tsuneo MATSUBARA, Nobuaki IKEDA, Takekuni IWATA, Nobuo INABA, Hiroyuki TSUJINO, Yuruko OKAMOTO, Keigo MAEHARA, Muneto YOSHIOKA, Yube IIDA, Hiroshi OKUBO, Yoshihiro UEDA, Fumio MIKI, Kenji TAKAMATSU, Osamu MIYAMOTO, Eiro TSUBURA, Toshihiro GOTO, Masakazu TAMURA, Rinzo SOEJIMA, Yoshito NIKI, Masatoshi WATANABE, Michio YAMAKIDO, Kanoko HAYASHI, Yoshiro SAWAE, Tomohiro KUSABA, Seizaburo KASHIWAGI, Akira UEDA, Kaoru OKADA, Michio ONO, Masahide TAKII, Takatoshi OTONARI, Kohei HARA, Keizo YAMAGUCHI, Yoji SUZUYAMA, Yoshiteru SHIGENO, Koichi WATANABE, Masao NAGASAWA, Keizo MATSUMOTO, Harumi SHISHIDO, Atsushi TAKAHASHI, Masakazu TAKASUGI, Shigeru TAMAKI, Hisao KIMURA, Masayuki ANDO, Mineharu SUGIMOTO, Moritaka SUGA, Katsumasa TOKUNAGA, Yasutsugu FUKUDA, and Haruhiko TOKUOMI

Subjects
Piperacillin, medicine.medical_specialty, Clinical Trials as Topic, Respiratory tract infections, business.industry, Amoxicillin, Lung abscess, General Medicine, Pneumonia, medicine.disease, Double blind, Aspoxicillin, Chronic disease, Double-Blind Method, Internal medicine, Chronic Disease, medicine, Humans, Lung Abscess, business, Respiratory Tract Infections, medicine.drug
Published
1985

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