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315 results on '"Koh, Angela S."'

6. High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform

Author

Tham, Cheng-Yong, Poon, LaiFong, Yan, TingDong, Koh, Javier Yu Peng, Ramlee, Muhammad Khairul, Teoh, Vania Swee Imm, Zhang, Suihan, Cai, Yi, Hong, Zebin, Lee, Gina S, Liu, Jin, Song, Hai Wei, Hwang, William Ying Khee, Teh, Bin Tean, Tan, Patrick, Xu, Lifeng, Koh, Angela S, Osato, Motomi, and Li, Shang

Subjects
Biological Sciences, Bioinformatics and Computational Biology, Genetics, Aging, Biotechnology, 2.1 Biological and endogenous factors, Underpinning research, 1.1 Normal biological development and functioning, Aetiology, Generic health relevance, Good Health and Well Being, Humans, Telomere, Shelterin Complex
Abstract

Telomeres are specialized nucleoprotein structures at the ends of linear chromosomes. The progressive shortening of steady-state telomere length in normal human somatic cells is a promising biomarker for age-associated diseases. However, there remain substantial challenges in quantifying telomere length due to the lack of high-throughput method with nucleotide resolution for individual telomere. Here, we describe a workflow to capture telomeres using newly designed telobaits in human culture cell lines as well as clinical patient samples and measure their length accurately at nucleotide resolution using single-molecule real-time (SMRT) sequencing. Our results also reveal the extreme heterogeneity of telomeric variant sequences (TVSs) that are dispersed throughout the telomere repeat region. The presence of TVSs disrupts the continuity of the canonical (5'-TTAGGG-3')n telomere repeats, which affects the binding of shelterin complexes at the chromosomal ends and telomere protection. These findings may have profound implications in human aging and diseases.

Published
2023

19. Current Frailty Knowledge, Awareness, and Practices Among Physicians following the 2022 European Consensus Document on Frailty in Cardiology

Author

Wong, Jie Jun, primary, Wang, Laureen Yi-Ting, additional, Hasegawa, Koji, additional, Ho, Kay Woon, additional, Huang, Zijuan, additional, Teo, Louis LY, additional, Tan, Jack Wei Chieh, additional, Kasahara, Kazuyuki, additional, Tan, Ru-San, additional, Ge, Junbo, additional, and Koh, Angela S, additional

Published
2024
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22. Characteristics of pulmonary artery strain assessed by cardiovascular magnetic resonance imaging and associations with metabolomic pathways in human ageing

Author

Zhang, Hongzhou, primary, Leng, Shuang, additional, Gao, Fei, additional, Kovalik, Jean-Paul, additional, Wee, Hai Ning, additional, Chua, Kee Voon, additional, Ching, Jianhong, additional, Allen, John C., additional, Zhao, Xiaodan, additional, Tan, Ru-San, additional, Wu, Qinghua, additional, Leiner, Tim, additional, Koh, Angela S., additional, and Zhong, Liang, additional

Published
2024
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26. The association between dietary omega-3 fatty acids and cardiovascular death: the Singapore Chinese Health Study

Author

Koh, Angela S, Pan, An, Wang, Renwei, Odegaard, Andrew O, Pereira, Mark A, Yuan, Jian-Min, and Koh, Woon-Puay

Subjects
Biomedical and Clinical Sciences, Nutrition and Dietetics, Heart Disease - Coronary Heart Disease, Cardiovascular, Complementary and Integrative Health, Prevention, Nutrition, Heart Disease, Aging, Clinical Research, 2.4 Surveillance and distribution, Aetiology, Stroke, Good Health and Well Being, Aged, Asian People, Cardiovascular Diseases, China, Diet, Docosahexaenoic Acids, Eicosapentaenoic Acid, Feeding Behavior, Female, Humans, Linear Models, Male, Middle Aged, Multivariate Analysis, Proportional Hazards Models, Protective Factors, Risk Assessment, Risk Factors, Singapore, Surveys and Questionnaires, Time Factors, alpha-Linolenic Acid, Cardiovascular diseases, Chinese, epidemiology, fatty acids, mortality, nutrition, Cardiovascular medicine and haematology
Abstract

BackgroundAlthough studies suggest that omega-3 fatty acids intake may reduce cardiovascular disease (CVD) mortality risk, few studies have differentiated dietary eicosapentaenoic/docosahexaenoic acid (EPA/DHA) from alpha-linolenic acid (ALA), and epidemiological research in Asian populations is limited.MethodsThe Singapore Chinese Health Study is a population-based cohort that recruited 63,257 Chinese adults aged 45-74 years from 1993 to 1998. Usual diet was measured at recruitment using a validated semiquantitative food-frequency questionnaire, and mortality information was identified via registry linkage up to 31 December 2011. Cox proportional hazard models were used to calculate hazard ratios (HRs) with adjustment for potential confounders.ResultsWe documented 4780 cardiovascular deaths (including 2697 coronary heart disease (CHD) deaths and 1298 stroke deaths) during 890,473 person-years of follow up. Omega-3 fatty acids intake was monotonically associated with reduced risk of cardiovascular mortality. Compared to the lowest quartile, the HR was 0.88 (95% confidence interval, CI, 0.81-0.96), 0.88 (95% CI 0.80-0.97), and 0.83 (95% CI 0.74-0.92) for the second, third, and highest quartile, respectively (p-trend = 0.003). Both EPA/DHA and ALA were independently associated with reduced risk of cardiovascular mortality: HR comparing extreme quartiles was 0.86 (95% CI 0.77-0.96, p-trend = 0.002) and 0.81 (95% CI 0.73-0.90, p-trend

Published
2015

29. Current frailty knowledge, awareness, and practices among physicians following the 2022 European consensus document on Frailty in Cardiology.

Author

Jie Jun Wong, Laureen Yi-Ting Wang, Koji Hasegawa, Kay Woon Ho, Zijuan Huang, Teo, Louis L.Y., Jack Wei Chieh Tan, Kazuyuki Kasahara, Ru-San Tan, Junbo Ge, and Koh, Angela S.

Subjects
FRAILTY, HEALTH care teams, PHYSICIANS, CARDIOLOGISTS, CARDIOLOGY, HEART failure
Abstract

Aims: Aging-related cardiovascular disease and frailty burdens are anticipated to rise with global aging. In response to directions from major cardiovascular societies, we investigated frailty knowledge, awareness, and practices among cardiologists as key stakeholders in this emerging paradigm a year after the European Frailty in Cardiology consensus document was published. Methods and results: We launched a prospective multinational web-based survey via social networks to broad cardiology communities representing multiple World Health Organization regions, including Western Pacific and Southeast Asia regions. Overall, 578 respondents [38.2% female; ages 35-49 years (55.2%) and 50-64 years (34.4%)] across subspecialties, including interventionists (43.3%), general cardiologists (30.6%), and heart failure specialists (HFSs) (10.9%), were surveyed. Nearly half had read the consensus document (38.9%). Non-interventionists had better perceived knowledge of frailty assessment instruments (fully or vaguely aware, 57.2% vs. 45%, adj. P = 0.0002), exercise programmes (well aware, 12.9% vs. 6.0%, adj. P = 0.001), and engaged more in multidisciplinary team care (frequently or occasionally, 52.6% vs. 41%, adj. P = 0.002) than interventionists. Heart failure specialists more often addressed pre-procedural frailty (frequently or occasionally, 43.5% vs. 28.2%, P = 0.004) and polypharmacy (frequently or occasionally, 85.5% vs. 71%, adj. P = 0.014) and had consistently better composite knowledge (39.3% vs. 21.6%, adj. P = 0.001) and practice responses (21% vs. 11.1%, adj. P = 0.018) than non-HFSs. Respondents with better knowledge responses also had better frailty practices (40.3% vs. 3.6%, adj. P < 0.001). Conclusion: Distinct response differences suggest that future strategies strengthening frailty principles should address practices peculiar to subspecialties, such as pre-procedural frailty strategies for interventionists and rehabilitation interventions for HFSs. [ABSTRACT FROM AUTHOR]

Published
2024
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