Search

Your search keyword '"Koebnik, Ralf [0000-0002-4419-0542]"' showing total 2 results
Start Over Author "Koebnik, Ralf [0000-0002-4419-0542]"
2 results on '"Koebnik, Ralf [0000-0002-4419-0542]"'

1. Comparative Genomics Identifies Conserved and Variable TAL Effectors in African Strains of the Cotton Pathogen Xanthomonas citri pv. malvacearum

Author

European Commission, Institut de Recherche pour le Développement (France), Universidad de Los Andes (Colombia), Pérez-Quintero, Alvaro. [0000-0003-3530-8251], Cuesta-Morrondo, Sara [0000-0002-8407-233X], Hakalová, Eliška [0000-0002-5433-8993], Betancurt-Anzola, Daniela [0000-0001-9160-8190], Valera, Laura Carolina Camelo [0000-0003-2569-681X], Chica Cardenas, Luis Alberto [0009-0007-7408-9385], Matiz-Céron, Luisa [0000-0001-8777-7047], Jacobs, Jonathan M. [0000-0002-1553-2013], Román-Reyna, Verónica [0000-0003-0072-8096], Reyes Muñoz, Alejandro [0000-0003-2907-3265], Bernal Giraldo, Adriana J. [0000-0002-3557-697X], Koebnik, Ralf [0000-0002-4419-0542], Pérez-Quintero, Alvaro., Rodriguez-R., Luis M., Cuesta-Morrondo, Sara, Hakalová, Eliška, Betancurt-Anzola, Daniela, Valera, Laura Carolina Camelo, Chica Cardenas, Luis Alberto, Matiz-Céron, Luisa, Jacobs, Jonathan M., Román-Reyna, Verónica, Reyes Muñoz, Alejandro, Bernal Giraldo, Adriana J., Koebnik, Ralf, European Commission, Institut de Recherche pour le Développement (France), Universidad de Los Andes (Colombia), Pérez-Quintero, Alvaro. [0000-0003-3530-8251], Cuesta-Morrondo, Sara [0000-0002-8407-233X], Hakalová, Eliška [0000-0002-5433-8993], Betancurt-Anzola, Daniela [0000-0001-9160-8190], Valera, Laura Carolina Camelo [0000-0003-2569-681X], Chica Cardenas, Luis Alberto [0009-0007-7408-9385], Matiz-Céron, Luisa [0000-0001-8777-7047], Jacobs, Jonathan M. [0000-0002-1553-2013], Román-Reyna, Verónica [0000-0003-0072-8096], Reyes Muñoz, Alejandro [0000-0003-2907-3265], Bernal Giraldo, Adriana J. [0000-0002-3557-697X], Koebnik, Ralf [0000-0002-4419-0542], Pérez-Quintero, Alvaro., Rodriguez-R., Luis M., Cuesta-Morrondo, Sara, Hakalová, Eliška, Betancurt-Anzola, Daniela, Valera, Laura Carolina Camelo, Chica Cardenas, Luis Alberto, Matiz-Céron, Luisa, Jacobs, Jonathan M., Román-Reyna, Verónica, Reyes Muñoz, Alejandro, Bernal Giraldo, Adriana J., and Koebnik, Ralf

Abstract

Strains of Xanthomonas citri pv. malvacearum cause bacterial blight of cotton, a potentially serious threat to cotton production worldwide, including in sub-Saharan countries. Development of disease symptoms, such as water soaking, has been linked to the activity of a class of type 3 effectors, called transcription activator-like (TAL) effectors, which induce susceptibility genes in the host's cells. To gain further insight into the global diversity of the pathogen, to elucidate their repertoires of TAL effector genes, and to better understand the evolution of these genes in the cotton-pathogenic xanthomonads, we sequenced the genomes of three African strains of X. citri pv. malvacearum using nanopore technology. We show that the cotton-pathogenic pathovar of X. citri is a monophyletic lineage containing at least three distinct genetic subclades, which appear to be mirrored by their repertoires of TAL effectors. We observed an atypical level of TAL effector gene pseudogenization, which might be related to resistance genes that are deployed to control the disease. Our work thus contributes to a better understanding of the conservation and importance of TAL effectors in the interaction with the host plant, which can inform strategies for improving resistance against bacterial blight in cotton.

Published
2023

2. Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary Regulated Xanthomonas

Author

Università degli Studi di Catania, Agencia Estatal de Investigación (España), European Commission, University of Zurich, Scottish Government, Catara, Vittoria [0000-0001-8076-258X], Cubero, Jaime [0000-0002-4314-857X], Pothier, Joël F. [0000-0002-9604-7780], Bosis, Eran [0000-0001-7762-5920], Bragard, Claude [0000-0003-3169-1716], Dermić, Edyta [0000-0002-0786-9062], Holeva, Maria C. [0000-0001-8645-5943], Jacques, Marie-Agnès [0000-0002-1442-917X], Petter, Francoise [0000-0001-9633-5830], Pruvost, Olivier [0000-0002-3175-9795], Robene, Isabelle [0000-0003-2012-4652], Studholme, David J. [0000-0002-3010-6637], Tavares, Fernando [0000-0001-9913-1155], Vicente, Joana G. [0000-0001-8442-5935], Koebnik, Ralf [0000-0002-4419-0542], Costa, Joana [0000-0001-7028-2873], Catara, Vittoria, Cubero, Jaime, Pothier, Joël F., Bosis, Eran, Bragard, Claude, Dermić, Edyta, Holeva, Maria C., Jacques, Marie-Agnès, Petter, Francoise, Pruvost, Olivier, Robene, Isabelle, Studholme, David J., Tavares, Fernando, Vicente, Joana G., Koebnik, Ralf, Costa, Joana, Università degli Studi di Catania, Agencia Estatal de Investigación (España), European Commission, University of Zurich, Scottish Government, Catara, Vittoria [0000-0001-8076-258X], Cubero, Jaime [0000-0002-4314-857X], Pothier, Joël F. [0000-0002-9604-7780], Bosis, Eran [0000-0001-7762-5920], Bragard, Claude [0000-0003-3169-1716], Dermić, Edyta [0000-0002-0786-9062], Holeva, Maria C. [0000-0001-8645-5943], Jacques, Marie-Agnès [0000-0002-1442-917X], Petter, Francoise [0000-0001-9633-5830], Pruvost, Olivier [0000-0002-3175-9795], Robene, Isabelle [0000-0003-2012-4652], Studholme, David J. [0000-0002-3010-6637], Tavares, Fernando [0000-0001-9913-1155], Vicente, Joana G. [0000-0001-8442-5935], Koebnik, Ralf [0000-0002-4419-0542], Costa, Joana [0000-0001-7028-2873], Catara, Vittoria, Cubero, Jaime, Pothier, Joël F., Bosis, Eran, Bragard, Claude, Dermić, Edyta, Holeva, Maria C., Jacques, Marie-Agnès, Petter, Francoise, Pruvost, Olivier, Robene, Isabelle, Studholme, David J., Tavares, Fernando, Vicente, Joana G., Koebnik, Ralf, and Costa, Joana

Abstract

Bacteria in the genus Xanthomonas infect a wide range of crops and wild plants, with most species responsible for plant diseases that have a global economic and environmental impact on the seed, plant, and food trade. Infections by Xanthomonas spp. cause a wide variety of non-specific symptoms, making their identification difficult. The coexistence of phylogenetically close strains, but drastically different in their phenotype, poses an added challenge to diagnosis. Data on future climate change scenarios predict an increase in the severity of epidemics and a geographical expansion of pathogens, increasing pressure on plant health services. In this context, the effectiveness of integrated disease management strategies strongly depends on the availability of rapid, sensitive, and specific diagnostic methods. The accumulation of genomic information in recent years has facilitated the identification of new DNA markers, a cornerstone for the development of more sensitive and specific methods. Nevertheless, the challenges that the taxonomic complexity of this genus represents in terms of diagnosis together with the fact that within the same bacterial species, groups of strains may interact with distinct host species demonstrate that there is still a long way to go. In this review, we describe and discuss the current molecular-based methods for the diagnosis and detection of regulated Xanthomonas, taxonomic and diversity studies in Xanthomonas and genomic approaches for molecular diagnosis.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results