Search

Your search keyword '"Kodde, L. P."' showing total 15 results
Start Over Author "Kodde, L. P."
15 results on '"Kodde, L. P."'

10. Efficient distinction of invasive aquatic plant species from non-invasive related species using DNA barcoding.

Author

Ghahramanzadeh, R., Esselink, G., Kodde, L. P., Duistermaat, H., Valkenburg, J. L. C. H., Marashi, S. H., Smulders, M. J. M., and Wiel, C. C. M.

Subjects
AQUATIC plants, PLANT species, GENETIC barcoding, MYRIOPHYLLUM, CHLOROPLASTS, MOLECULAR genetics
Abstract

Biological invasions are regarded as threats to global biodiversity. Among invasive aliens, a number of plant species belonging to the genera Myriophyllum, Ludwigia and Cabomba, and to the Hydrocharitaceae family pose a particular ecological threat to water bodies. Therefore, one would try to prevent them from entering a country. However, many related species are commercially traded, and distinguishing invasive from non-invasive species based on morphology alone is often difficult for plants in a vegetative stage. In this regard, DNA barcoding could become a good alternative. In this study, 242 samples belonging to 26 species from 10 genera of aquatic plants were assessed using the chloroplast loci trn H- psb A, mat K and rbc L. Despite testing a large number of primer sets and several PCR protocols, the mat K locus could not be amplified or sequenced reliably and therefore was left out of the analysis. Using the other two loci, eight invasive species could be distinguished from their respective related species, a ninth one failed to produce sequences of sufficient quality. Based on the criteria of universal application, high sequence divergence and level of species discrimination, the trn H-psb A noncoding spacer was the best performing barcode in the aquatic plant species studied. Thus, DNA barcoding may be helpful with enforcing a ban on trade of such invasive species, such as is already in place in the Netherlands. This will become even more so once DNA barcoding would be turned into machinery routinely operable by a nonspecialist in botany and molecular genetics. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

11. Microsatellite markers spanning the apple (Malusx domesticaBorkh.) genome

Author

Silfverberg-Dilworth, E., Matasci, C., Weg, W., Kaauwen, M., Walser, M., Kodde, L., Soglio, V., Gianfranceschi, L., Durel, C., Costa, F., Yamamoto, T., Koller, B., Gessler, C., and Patocchi, A.

Abstract

A new set of 148 apple microsatellite markers has been developed and mapped on the apple reference linkage map Fiesta x Discovery. One-hundred and seventeen markers were developed from genomic libraries enriched with the repeats GA, GT, AAG, AAC and ATC; 31 were developed from EST sequences. Markers derived from sequences containing dinucleotide repeats were generally more polymorphic than sequences containing trinucleotide repeats. Additional eight SSRs from published apple, pear, and Sorbus torminalisSSRs, whose position on the apple genome was unknown, have also been mapped. The transferability of SSRs across Maloideaespecies resulted in being efficient with 41% of the markers successfully transferred. For all 156 SSRs, the primer sequences, repeat type, map position, and quality of the amplification products are reported. Also presented are allele sizes, ranges, and number of SSRs found in a set of nine cultivars. All this information and those of the previous CH-SSR series can be searched at the apple SSR database (http://www.hidras.unimi.it) to which updates and comments can be added. A large number of apple ESTs containing SSR repeats are available and should be used for the development of new apple SSRs. The apple SSR database is also meant to become an international platform for coordinating this effort. The increased coverage of the apple genome with SSRs allowed the selection of a set of 86 reliable, highly polymorphic, and overall the apple genome well-scattered SSRs. These SSRs cover about 85% of the genome with an average distance of one marker per 15 cM.A new set of 148 apple microsatellite markers has been developed and mapped on the apple reference linkage map Fiesta x Discovery. One-hundred and seventeen markers were developed from genomic libraries enriched with the repeats GA, GT, AAG, AAC and ATC; 31 were developed from EST sequences. Markers derived from sequences containing dinucleotide repeats were generally more polymorphic than sequences containing trinucleotide repeats. Additional eight SSRs from published apple, pear, and Sorbus torminalisSSRs, whose position on the apple genome was unknown, have also been mapped. The transferability of SSRs across Maloideaespecies resulted in being efficient with 41% of the markers successfully transferred. For all 156 SSRs, the primer sequences, repeat type, map position, and quality of the amplification products are reported. Also presented are allele sizes, ranges, and number of SSRs found in a set of nine cultivars. All this information and those of the previous CH-SSR series can be searched at the apple SSR database (http://www.hidras.unimi.it) to which updates and comments can be added. A large number of apple ESTs containing SSR repeats are available and should be used for the development of new apple SSRs. The apple SSR database is also meant to become an international platform for coordinating this effort. The increased coverage of the apple genome with SSRs allowed the selection of a set of 86 reliable, highly polymorphic, and overall the apple genome well-scattered SSRs. These SSRs cover about 85% of the genome with an average distance of one marker per 15 cM.

Published
2006
Full Text
View/download PDF

15. An overview of the position and robustness of scab resistance QTLs and major genes by aligning genetic maps of five apple progenies

Author

Cesare Gessler, R. Liebhard, Fanny Calenge, L.P. Kodde, Stefano Tartarini, Luciana Parisi, Fabio Gennari, W.E. Van De Weg, M. Thiermann, Frank Dunemann, Yves Lespinasse, C.E. Durel, ProdInra, Migration, Unité mixte de recherche génétique et horticulture Genhort, Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-Institut National d'Horticulture, Unité de recherche Pathologie végétale et phytobactériologie, Institut National de la Recherche Agronomique (INRA), LAURENS F. AND EVANS K., Durel, C. E., Calenge, F., Parisi, L., van de Weg, W. E., Kodde, L. P., Liebhard, R., Gessler, C., Thiermann, M., Dunemann, F., Gennari, F., Tartarini, S., and Lespinasse, Y.

Subjects
0106 biological sciences, Quantitative trait loci, [SDV]Life Sciences [q-bio], Population, Horticulture, Biology, Quantitative trait locus, 01 natural sciences, 03 medical and health sciences, Broad spectrum, Quantitative resistance, Genetic map, PRI Biodiversiteit en Veredeling, education, MALUS X DOMESTICA, Gene, Malus x domestica, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Genetics, 0303 health sciences, education.field_of_study, QUANTITATIVE TRAIT LOCI, Venturia inaequalis, Robustness (evolution), food and beverages, Molecular markers, biology.organism_classification, SSR, PRI Biodiversity and Breeding, [SDV] Life Sciences [q-bio], GENE MAJEUR DE RESISTANCE, Genetic marker, Malus x domestica, Venturia inaequalis, quantitative resistance, quantitative trait loci, genetic map, molecular markers, Amplified fragment length polymorphism, 010606 plant biology & botany
Abstract

In the frame of the D.A.R.E. project, five mapping populations have been studied for partial scab resistance against several races of Venturia inaequalis. A main objective was to identify QTLs (quantitative traits loci) with broad spectrum of resistance towards a wide range of strains of the fungus. Genetic markers (mainly SSR and AFLP) were tested on each population and genetic maps were constructed for both parents of each population. Meanwhile, pathological tests with several isolates of different races of V. inaequalis were performed. Four major genomic regions appear to be involved in scab resistance: they are located on linkage groups (LG) LG-1, LG-2, LG-11, and LG-17. Some other linkage groups carry either QTLs or major resistance genes that are isolate specific: a QTL on LG-5, Vd on LG-10, and Vg on LG-12. The QTL region located on LG-17 clearly exhibited the widest spectrum of resistance

Published
2004

Catalog

Books, media, physical & digital resources
See catalog results