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29 results on '"Kochkin, D. V."'

21. Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea Deltoidea, Tribulus Terrestris and Panax Japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

Author

Povydysh, M. N., Titova, M. V., Ivanov, I. M., Klushin, A. G., Kochkin, D. V., Galishev, B. A., Popova, E. V., Ivkin, D. Yu., Luzhanin, V. G., Krasnova, M. V., Demakova, N. V., and Nosov, A. M.

Subjects
DRUG EFFECT, DIABETES MELLITUS, EXPERIMENTAL, BLOOD, ADRENALINE MODEL, STREPTOZOTOCIN-INDUCED TYPE 2 DIABETES MELLITUS, DIOSCOREA, EXPERIMENTAL DIABETES MELLITUS, BIOMASS, CARBOHYDRATE METABOLISM, CELL CULTURE TECHNIQUE, RATS, PLANTS, MEDICINAL, HYPERGLYCEMIA, PLANT EXTRACT, PLANT EXTRACTS, PLANT CELL CULTURE, DIURESIS, FUROSTANOL-TYPE GLYCOSIDES, LIPID METABOLISM, CHOLESTEROL, MEDICINAL PLANT, ANIMALS, ANIMAL, CELL CULTURE TECHNIQUES, DIABETES MELLITUS, TYPE 2, BLOOD GLUCOSE, NON INSULIN DEPENDENT DIABETES MELLITUS, RAT, HEMATURIA, YAM, GLUCOSE BLOOD LEVEL, TRIBULUS, BIOREACTORS, BIOREACTOR, PANAX
Abstract

In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species Dioscorea deltoidea, Tribulus terrestris and Panax japonicus to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1–2.5 h (D. deltoidea and T. terrestris) or 1 h (P. japonicus) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of D. deltoidea and T. terrestris acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39– 57%), blood-glucose level (by 46–51%), blood content in urine (by 78–80%) and total cholesterol (25– 36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in D. deltoidea; furostanol-type steroidal glycosides and quinic acid derivatives in T. terrestris; and ginsenosides and malonyl-ginsenosides in P. japonicus. These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This. Funding: Bioreactor cultivation of plant cell suspensions and their biochemical analysis were financially supported by the Ministry of Science and Higher Education of Russian Federation through Megagrant project no. 075-15-2019-1882 and performed by using the equipment of the large-scale research facilities “Experimental biotechnological facility” and “All-Russian Collection of cell cultures of higher plants” of the IPPRAS (EBF IPPRAS and ARCCC HP IPPRAS). Hypoglycemic activity evaluation of the cell biomass was performed with the financial support of the Russian Science Foundation project no. 19-14-00387. The results of the hypoglycemic activity evaluation were obtained by using the equipment of the Center for Collective Use “Analytical Center of the Federal State Budgetary Educational Institution of Higher Education Saint Petersburg State Chemical Pharmaceutical University (SPCPU) of the Ministry of Health of Russia”, equipped with the financial support of the Ministry of Education and Science of Russia.

Published
2021

28. [Ginsenosides affect the system of Toll-like receptors in the brain of rats under conditions of long-term alcohol withdrawal].

Author

Airapetov MI, Eresko SO, Kochkin DV, Nosov AM, Bychkov ER, Lebedev AA, and Shabanov PD

Subjects
Rats, Animals, Toll-Like Receptor 4 genetics, Toll-Like Receptors genetics, Brain, RNA, Messenger metabolism, Ginsenosides metabolism, Ginsenosides pharmacology, Alcoholism, Substance Withdrawal Syndrome
Abstract

Long-term alcohol consumption causes the development of neuroinflammation in various brain structures. One of the mechanisms involved in this process is the increased activity of TLR-signaling intracellular pathways. Studies confirm the ability of ginseng extract or its individual ginsenosides to reduce the increased activity of TLR-signaling pathways. The aim of our study was to study the effect of the amount of ginsenosides obtained from the extract of the Panax japonicus cell line on the state of the TLR-signaling system in the nucleus accumbens and hippocampus of the rat brain in a model of long-term alcohol consumption during alcohol withdrawal. The results of the study showed that ginsenosides were able to make changes in the TLR signaling system, which has been altered by long-term alcohol consumption. A significant effect of ginsenosides on the level of TLR3 and TLR4 mRNA in the nucleus accumbens was found, while in the hippocampus, ginsenosides significantly affected the level of TLR7 mRNA. The effect of ginsenosides on the level of mRNA of transcription factors and cytokines involved in TLR-signaling was evaluated. Thus, results of our study confirm that ginsenosides are able to influence the state of TLR-signaling pathways, but this effect is multidirectional in relation to different brain structures. In the future, it seems interesting to evaluate the role of individual ginsenosides in relation to genes of TLR-signaling, as well as the effect of ginsenosides on other brain structures.

Published
2022
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29. [Formation of protodioscin and deltoside isomers in suspension cultures of Nepal yam (Dioscorea deltoidea Wall.) cells].

Author

Khandy MT, Titova MV, Konstantinova SV, Kochkin DV, Ivanov IM, and Nosov AM

Subjects
Dioscorea cytology, Dioscorea metabolism, Diosgenin analogs & derivatives, Glycosides biosynthesis, Plant Cells metabolism, Saponins biosynthesis
Abstract

Changes in the content of the furostanol glycosides protodioscin and deltoside, particularly that of the (25S)-isomers of the glycosides, during suspension cultivation of different lines of Nepal yam (Dioscorea deltoidea Wall.) cells of the strain IFR-DM-0.5 has been investigated. The composition of furostanol glycosides has been characterized, and the dynamics of the accumulation of individual glycosides during lengthy subcultivation of cells maintained in flasks or in a barbotage bioreactor has been analyzed. A positive correlation between the growth and accumulation of substances that belonged to the class of furostanol glycosides has been demonstrated for cultured dioscorea cells, whereas the content of some of the individual glycosides varied considerably between the lines of the strain, cultures maintained under different conditions, and even between cells in different phases of the growth cycle. The increased content of (25R)-forms of the glycosides (protodioscin and deltoside) was correlated with a decrease in the cellular growth rate, whereas an increase in culture growth intensity occurred concomitantly to an increase of the amount of (25S)-isomers. This may be indicative of the specific stimulatory effect of (25S)-glycosides, but not the (25R)-forms, on cell proliferation in vitro. Thus, the concentration of (25S)-forms may increase due to the autoselection of cells capable of intensive division during prolonged cultivation.

Published
2016

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