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1. Bioprinting for Skin

Author

Koch, Lothar, primary, Michael, Stefanie, additional, Reimers, Kerstin, additional, Strauß, Sarah, additional, Vogt, Peter M., additional, and Chichkov, Boris, additional

Published
2022
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2. List of contributors

Author

Atala, Anthony, primary, Azhari, Amir, additional, Basalah, Ahmad, additional, Berry, David, additional, Bonilla, Diana, additional, Castro, Nathan J., additional, Chang, Robert C., additional, Chen, Shaochen, additional, Chichkov, Boris, additional, Chrisey, Douglas B., additional, Copus, Joshua, additional, Corr, David T., additional, Dean, David, additional, Deng, Heng, additional, Dias, Andrew D., additional, Dorthé, Erik W., additional, D’Lima, Darryl D., additional, Ferrill, Elizabeth D., additional, Fisher, John P., additional, Grayson, Warren, additional, Grigoryan, Bagrat, additional, Grogan, Shawn P., additional, Hann, Sung Yun, additional, Holmes, Benjamin, additional, Huang, Yong, additional, Kandel, Rita, additional, Kimicata, Megan, additional, Kingsley, David M., additional, Koch, Lothar, additional, Kopcow, Joel, additional, Larsen, Michael, additional, Lee, Jia Min, additional, Lee, Sang Jin, additional, Lin, Jian, additional, Ma, Wai Cheung, additional, Ma, Xuanyi, additional, Maharjan, Sushila, additional, Michael, Stefanie, additional, Miller, Jordan S., additional, Miller, Kathleen, additional, Miller, Michael, additional, Mishra, Ruchi, additional, O’Brien, Christopher, additional, Phamduy, Theresa B., additional, Placone, Jesse K., additional, Rajan, Kai, additional, Reimers, Kerstin, additional, Roberge, Cassandra L., additional, Saksena, Jayant, additional, Sarkar, Naboneeta, additional, Shen, Yin-Lin, additional, Shirwaiker, Rohan, additional, Sklare, S.C., additional, Starly, Binil, additional, Strauß, Sarah, additional, Tang, Min, additional, Tourlomousis, Filippos, additional, Toyserkani, Ehsan, additional, Vlasea, Mihaela, additional, Vogt, Peter M., additional, Yeong, Wai Yee, additional, Zhang, Lijie Grace, additional, Zhang, Yu Shrike, additional, Zhou, Yuxiao, additional, and Zhu, Wei, additional

Published
2022
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3. Beiträge zum 29. Darmstädter Geotechnik-Kolloquium am 7. März 2024

Author

Zachert, Hauke, Kochnev, Alexander, Siegel, Simon, Black, Christoph, Wall, Johannes, Dallinger, Christina, Rebhan, Matthias J., Daxer, Hans-Peter, Tschuchnigg, Franz, Marte, Roman, Schuch, Markus, Klass, Clemens, Breit, Klaus, Beckhaus, Karsten, Ibuk, Hursit, Jäger, Stefan, Labenski, Johannes, Poenaru, Alexandru, Treffeisen, Torben, Schnell, Sebastian, Sklorz, Michael, Schöppner, Clemens, Jaramillo Castro, July Ellen, Henke, Sascha, Detert, Oliver, Daubner, Marcus, Schneider, Marc, Hell, Marylin, Wittekoek, Britt, Eekelen, Suzanne J. M. van, Schauber, Pascal, Pandrea, Paul, Topolnicki, Michał, Makowska, Karolina, Baumgartner, Lorenz, Kasper, Tobias, Cagimda, Inan, Karpa, Gudrun, Fontara, Ioanna-Kleonik, Appel, Silke, Wegerl, Markus, Baur, Raphael, Voigt, Jens, Koch, Lothar, Maier-Rotter, Mona, Gündling, Norbert, Gutwald, Jörg, Niemetz, Gunther, Wehr, Christoph, Böhm, Sebastian, Zachert, Hauke, Kochnev, Alexander, Siegel, Simon, Black, Christoph, Wall, Johannes, Dallinger, Christina, Rebhan, Matthias J., Daxer, Hans-Peter, Tschuchnigg, Franz, Marte, Roman, Schuch, Markus, Klass, Clemens, Breit, Klaus, Beckhaus, Karsten, Ibuk, Hursit, Jäger, Stefan, Labenski, Johannes, Poenaru, Alexandru, Treffeisen, Torben, Schnell, Sebastian, Sklorz, Michael, Schöppner, Clemens, Jaramillo Castro, July Ellen, Henke, Sascha, Detert, Oliver, Daubner, Marcus, Schneider, Marc, Hell, Marylin, Wittekoek, Britt, Eekelen, Suzanne J. M. van, Schauber, Pascal, Pandrea, Paul, Topolnicki, Michał, Makowska, Karolina, Baumgartner, Lorenz, Kasper, Tobias, Cagimda, Inan, Karpa, Gudrun, Fontara, Ioanna-Kleonik, Appel, Silke, Wegerl, Markus, Baur, Raphael, Voigt, Jens, Koch, Lothar, Maier-Rotter, Mona, Gündling, Norbert, Gutwald, Jörg, Niemetz, Gunther, Wehr, Christoph, and Böhm, Sebastian

Abstract

Themenschwerpunkte: 1. Klimawandelfolgen und Nachhaltigkeit 2. Digitalisierung und künstliche Intelligenz 3. Planung und Bemessung von Infrastrukturprojekten 4. Großprojekte

Published
2024

5. Epilog

Author

Koch, Lothar Nishkam, Dietrich, Wolfgang, Series editor, and Koch, Lothar Nishkam

Published
2018
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13. Laser-Based Cell Printing

Author

Koch, Lothar, Deiwick, Andrea, Chichkov, Boris, Ovsianikov, Aleksandr, editor, Yoo, James, editor, and Mironov, Vladimir, editor

Published
2018
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15. Laser bioprinting of human iPSC-derived neural stem cells and neurons: Effect on cell survival, multipotency, differentiation, and neuronal activity

Author

Koch, Lothar, Deiwick, Andrea, Soriano, Jordi, Chichkov, Boris, Koch, Lothar, Deiwick, Andrea, Soriano, Jordi, and Chichkov, Boris

Abstract

Generation of human neuronal networks by three-dimensional (3D) bioprinting is promising for drug testing and hopefully will allow for the understanding of cellular mechanisms in brain tissue. The application of neural cells derived from human induced-pluripotent stem cells (hiPSCs) is an obvious choice, since hiPSCs provide access to cells unlimited in number and cell types that could be generated by differentiation. The questions in this regard include which neuronal differentiation stage is optimal for printing of such networks, and to what extent the addition of other cell types, especially astrocytes, supports network formation. These aspects are the focus of the present study, in which we applied a laser-based bioprinting technique and compared hiPSC-derived neural stem cells (NSCs) with neuronal differentiated NSCs, with and without the inclusion of co-printed astrocytes. In this study, we investigated in detail the effects of cell types, printed droplet size, and duration of differentiation before and after printing on viability, as well as proliferation, stemness, differentiation potential, formation of dendritic extensions and synapses, and functionality of the generated neuronal networks. We found a significant dependence of cell viability after dissociation on differentiation stage, but no impact of the printing process. Moreover, we observed a dependence of the abundance of neuronal dendrites on droplet size, a marked difference between printed cells and normal cell culture in terms of further differentiation of the cells, especially differentiation into astrocytes, as well as neuronal network formation and activity. Notably, there was a clear effect of admixed astrocytes on NSCs but not on neurons.

Published
2023

32. Development of in vitro 3D brain models on laser fabricated scaffolds

Author

Koroleva, Anastasia, El-Tamer, Ayman, Deiwick, Andrea, Koch, Lothar, Soriano, Jordi, Hinze, Ulf, and Chichkov, Boris

Subjects
nervous system
Abstract

In this work, we present the design and implementation of a 3D scaffold platform that supports and promotes intricate neuronal network development. 3D scaffolds were produced by two-photon polymerization (2PP), a high-resolution 3D laser microstructuring technology, using the biocompatible and non-degradable photopolymer. Human iPSC-derived neuronal networks have developed and interconnected in a 3D environment shaped by vertically stacked scaffold layers. The iPSC-derived neuronal progenitor cells could develop into cortical projection neurons (CNPs) of all six layers, different types of inhibitory neurons and glia. The network showed strong neuronal spontaneous activity that combined individual and collective signaling events. Our results highlight advanced microstructured 3D scaffolds as a reliable platform for 3D in vitro modelling of neuronal functions., Transactions on Additive Manufacturing Meets Medicine, Vol 3 No 1 (2021): Trans. AMMM

Published
2021
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33. List of Contributors

Author

Azhari, Amir, primary, Basalah, Ahmad, additional, Castro, Nathan J., additional, Chang, Robert C., additional, Chen, Shaochen, additional, Chichkov, Boris, additional, Chrisey, Douglas B., additional, Corr, David T., additional, Curly, J. Lowry, additional, D’Lima, Darryl D., additional, Dean, David, additional, Dias, Andrew D., additional, Dorafshar, Amir, additional, Dorthé, Erik W., additional, Fisher, John P., additional, Grayson, Warren L., additional, Grigoryan, Bagrat, additional, Grogan, Shawn P., additional, Holmes, Benjamin, additional, Hornick, John F., additional, Huang, Yong, additional, Hung, Ben P., additional, Huri, Pinar Yilgor, additional, Kandel, Rita, additional, Kingsley, David M., additional, Koch, Lothar, additional, Larsen, Michael, additional, Li, Wei, additional, Ma, Xuanyi, additional, Michael, Stefanie, additional, Miller, Jordan S., additional, Miller, Michael, additional, Min, Lee Jia, additional, Mishra, Ruchi, additional, O’Brien, Christopher, additional, Ock, JinGyu, additional, Phamduy, Theresa B., additional, Placone, Jesse K., additional, Rajan, Kai, additional, Reimers, Kerstin, additional, Edgar, Tan Yong Sheng, additional, Shirwaiker, Rohan, additional, Sklare, S.C., additional, Starly, Binil, additional, Temple, Joshua P., additional, Tourlomousis, Filippos, additional, Toyserkani, Ehsan, additional, Vlasea, Mihaela, additional, Vogt, Peter M., additional, Yee, Yeong Wai, additional, Zhang, Lijie Grace, additional, Zhu, Wei, additional, and Zicheng, Zhu, additional

Published
2015
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34. Bioprinting for Skin

Author

Koch, Lothar, primary, Michael, Stefanie, additional, Reimers, Kerstin, additional, Vogt, Peter M., additional, and Chichkov, Boris, additional

Published
2015
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40. Capillary-like Formations of Endothelial Cells in Defined Patterns Generated by Laser Bioprinting

Author

Koch, Lothar, Deiwick, Andrea, Chichkov, Boris, Koch, Lothar, Deiwick, Andrea, and Chichkov, Boris

Abstract

Bioprinting is seen as a promising technique for tissue engineering, with hopes of one day being able to produce whole organs. However, thick tissue requires a functional vascular network, which naturally contains vessels of various sizes, down to capillaries of ~10 µm in diameter, often spaced less than 200 µm apart. If such thick tissues are to be printed, the vasculature would likely need to be printed at the same time, including the capillaries. While there are many approaches in tissue engineering to produce larger vessels in a defined manner, the small capillaries usually arise only in random patterns by sprouting from the larger vessels or from randomly distributed endothelial cells. Here, we investigated whether the small capillaries could also be printed in predefined patterns. For this purpose, we used a laser-based bioprinting technique that allows for the combination of high resolution and high cell density. Our aim was to achieve the formation of closed tubular structures with lumina by laser-printed endothelial cells along the printed patterns on a surface and in bioprinted tissue. This study shows that such capillaries are directly printable; however, persistence of the printed tubular structures was achieved only in tissue with external stimulation by other cell types.

Published
2021

43. Use of secondary ion mass spectrometry in nuclear forensic analysis for the characterization of plutonium and highly enriched uranium particles

Author

Betti, Maria, Tamborini, Gabriele, and Koch, Lothar

Subjects
Secondary ion mass spectrometry -- Usage, Mass spectrometry -- Forensic applications, Plutonium -- Research, Uranium -- Research, Chemistry
Abstract

The application of secondary ion mass spectrometry (SIMS) analysis is described for the characterization of plutonium and highly enriched uranium (HEU) particles with a diameter to 10 [[micro]meter]. Applying a method previously described, particles of HEU could be detected in a scrap material, together with natural uranium. The isotopic composition of the particles was measured with a typical accuracy and precision of 0.5%. The spectrum of the trace elements in the uranium particles was also recorded. From the results it was possible to deduce that the uranium oxide, as U[O.sub.2], was produced via a pyrochemical process. In a sample consisting of a mixture of three different species of particles, two of these were identified as plutonium particles. They were characterized according to their isotopic ratio 239/240 as well as to their dimension and shape. The results obtained by SIMS for the isotopic ratio were compared with those obtained analyzing the particles by Thermal Ionization Mass Spectrometry (TIMS). The shape and dimensions were confirmed by the analysis with Scanning Electron Microscopy (SEM). In both the cases the results obtained by SIMS were in good agreement with those from TIMS and SEM.

Published
1999

47. Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting

Author

Deiwick Andrea, Koch Lothar, Unger Claudia, Gruene Martin, and Chichkov Boris

Subjects
Medical technology, R855-855.5
Abstract

Abstract Background Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities. Methods Time-resolved imaging of the hydrogel jet dynamics and quantitative assessment of the dependence of printed droplet diameter on the process characteristics were conducted. Results The existence of a counterjet was visualised, proving the bubble collapsing theory for the jet formation. Furthermore, by adjusting the viscosity and height of the applied hydrogel layer in combination with different laser pulse energies, the printing of volumes in the range of 10 to 7000 picolitres was demonstrated. Additionally, the relationship between the viscosity and the layer thickness at different laser pulse energies on the printed droplet volume was identified. Conclusions These findings are essential for the advancement of laser-assisted bioprinting by enabling predictable printing results and the integration of computational methods in the generation of 3 D multi-cellular constructs.

Published
2011
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