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30 results on '"Kobatake, Reiko"'

9. miRNA‐21 deficiency impairs alveolar socket healing in mice

Author

Strauss, Franz Josef; https://orcid.org/0000-0002-5832-7327, Stähli, Alexandra; https://orcid.org/0000-0002-5631-3300, Kobatake, Reiko, Tangl, Stefan; https://orcid.org/0000-0002-9023-4745, Heimel, Patrick; https://orcid.org/0000-0002-2499-325X, Apaza Alccayhuaman, Karol Alí; https://orcid.org/0000-0003-4565-5222, Schosserer, Markus; https://orcid.org/0000-0003-2025-0739, Hackl, Matthias; https://orcid.org/0000-0002-4136-7293, Grillari, Johannes; https://orcid.org/0000-0001-5474-6332, Gruber, Reinhard; https://orcid.org/0000-0001-5400-9009, Strauss, Franz Josef; https://orcid.org/0000-0002-5832-7327, Stähli, Alexandra; https://orcid.org/0000-0002-5631-3300, Kobatake, Reiko, Tangl, Stefan; https://orcid.org/0000-0002-9023-4745, Heimel, Patrick; https://orcid.org/0000-0002-2499-325X, Apaza Alccayhuaman, Karol Alí; https://orcid.org/0000-0003-4565-5222, Schosserer, Markus; https://orcid.org/0000-0003-2025-0739, Hackl, Matthias; https://orcid.org/0000-0002-4136-7293, Grillari, Johannes; https://orcid.org/0000-0001-5474-6332, and Gruber, Reinhard; https://orcid.org/0000-0001-5400-9009

Abstract

Objectives MicroRNAs (miRNAs) are small noncoding RNAs demonstrated as critical post‐transcriptional modulators in dental tissues and bone regeneration, particularly miR‐21‐5p. However, the role of miR‐21‐5p in the healing of alveolar sockets following tooth extraction remains unknown. In this study we evaluated the influence of miR‐21‐5p in the healing of alveolar socket after tooth extraction. Methods Eight miR‐21‐5p knockout mice and eight littermate controls underwent tooth extraction of the upper right incisor. After a healing period of 14 days microCT and histological analyses were performed. Results MicroCT analysis showed that the percentage of bone in the extraction socket was significantly higher in the control group than in the miR‐21 knockout mice; either in the coronal (39.0%, CI 31.8 to 48.0 versus 23.0%, CI 17.8 to 35.2, P = 0.03) or in the middle part of the alveolar socket (56.0%, CI 50.9 to 62.5 versus 43.5% CI 28.6 to 54.6, P = 0.03). These differences were not noted in the apical part of the extraction socket. Histological analysis supported the microCT findings. Newly bone volume per tissue volume (BV/TV) was significantly higher in the control group when compared to miR‐21 knockout mice, 27.4% (CI 20.6 to 32.9) versus 19.0% (CI 14.7 to 21.5, P < 0.05), respectively. Surprisingly, no evident signs of buccal bone resorption were observed in both groups. Conclusion Despite the limitation of one observation period, these findings suggest that miR‐21‐5p delays the early healing of alveolar socket following tooth extraction. Whether miR‐21‐5p is essential for healing of alveolar sockets remains to be elucidated.

Published
2020

14. miRNA‐21 deficiency impairs alveolar socket healing in mice

Author

Strauss, Franz Josef, primary, Stähli, Alexandra, additional, Kobatake, Reiko, additional, Tangl, Stefan, additional, Heimel, Patrick, additional, Apaza Alccayhuaman, Karol Alí, additional, Schosserer, Markus, additional, Hackl, Matthias, additional, Grillari, Johannes, additional, and Gruber, Reinhard, additional

Published
2020
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18. Acid bone lysates reduce bone regeneration in rat calvaria defects.

Author

Strauss, Franz‐Josef, Kuchler, Ulrike, Kobatake, Reiko, Heimel, Patrick, Tangl, Stefan, and Gruber, Reinhard

Abstract

Acid bone lysates (ABLs) represent the growth factors and other molecules released during autologous graft resorption. However, the impact of these bone‐derived growth factors on the healing of bone defects has not yet been investigated. The aim of the present study was, therefore, to examine the impact of ABLs adsorbed to collagen membranes on bone regeneration. To this end, in 16 female Sprague Dawley rats, a standardized 5‐mm‐diameter critical size defect on the calvarial bone was created. The defects were covered with collagen membranes that had been soaked either in serum‐free media or ABLs followed by lyophilization. After a healing period of 4 weeks, micro‐computed tomography (μCT) and histological analyses by means of undecalcified thin ground sections were performed. μCT analysis of the inner 4 mm of the calvaria defect showed a greater bone defect coverage in the control group when compared to ABL group, 29.8% (confidence interval [CI]: 17.7–50.3) versus 5.6% (CI: 1.0–29.8, p =.03), respectively. Moreover, we found significantly more absolute bone volume (BV) in the control group when compared to ABL group, 0.59 mm3 (CI: 0.27–1.25) versus 0.07 mm3 (CI: 0.06–0.59, p =.04), respectively. Histomorphometry confirmed these findings with a relative BV in the central compartment of 14.1% (CI: 8.4–20.6) versus 5.6% (CI: 3.4–7.9, p =.004), respectively. These findings indicate that bone‐derived growth factors contained in ABLs are able to attenuate bone regeneration within collagen membranes. [ABSTRACT FROM AUTHOR]

Published
2021
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23. Use of an intraoral scanner to evaluate oral health.

Author

Kazuya Doi, Chihiro Yoshiga, Reiko Kobatake, Maiko Kawagoe, Kaien Wakamatsu, Kazuhiro Tsuga, Doi, Kazuya, Yoshiga, Chihiro, Kobatake, Reiko, Kawagoe, Maiko, Wakamatsu, Kaien, and Tsuga, Kazuhiro

Subjects
DENTAL hygiene, SCANNING systems, ORAL health, DENTAL plaque, INTRINSIC optical imaging, DENTAL technology, THREE-dimensional imaging, COMPUTER-aided design, DENTAL casting
Abstract

The purpose of this study was to determine if intraoral scanners (IOS) are useful for dental hygiene instruction. The dental plaque of eight volunteers with healthy dentition was stained with a plaque-disclosing solution, and the O'Leary Plaque Control Record (PCR) was measured by direct observation and by evaluating IOS images. PCR values were higher for IOS images than for direct observation. The difference was greatest for the lingual surface of mandibular anterior teeth. Use of IOS for dental plaque examination might be useful as a novel method for dental hygiene instruction. [ABSTRACT FROM AUTHOR]

Published
2021
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30. Evaluation of blue and red two-tone colored staining solution for dental plaque imaging using an intraoral scanner.

Author

Yoshiga C, Kobatake R, Doi K, Oue H, Kawagoe M, and Tsuga K

Abstract

Background: We first reported that intraoral imaging with a color-imaging intraoral scanner could evaluate stained plaques and contribute to oral hygiene instructions. In this study, we aimed to evaluate the effects of red and blue staining on dental hygiene assessment with an intraoral scanner using plaque control record values and plaque-stained areas., Material and Methods: Fifteen patients (six males and nine females, aged 25-85 years) were included in this study. The patients' teeth were stained with a two-tone (blue and red) dental plaque staining solution, and images of their teeth were recorded with an intraoral scanner and a digital camera. The plaque control record was measured by direct viewing, as usual, and on a monitor using intraoral scanner image. In addition, the plaque deposition area was measured using images obtained using an intraoral scanner and images taken by a digital camera., Results: Most parts were stained red and blue dental plaque staining was also observed. Plaque control record values tended to be higher in the intraoral scanner evaluation than in the direct evaluation. The plaque-stained area was larger in intraoral scanner images than in camera images., Conclusions: It is possible to use a two-tone plaque-staining solution for oral health evaluation using an intraoral scanner. In the future, we need to investigate cases of blue-stained plaques. Key words: Intraoral scanner, oral hygiene, dental plaque, preventive dentistry., Competing Interests: The authors declare that they have no competing interests., (Copyright: © 2024 Medicina Oral S.L.)

Published
2024
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