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The helical duplex architecture of DNA was discovered by Francis Crick and James Watson in 1951 and is well known and understood. However, nucleic acids can also adopt alternative structural conformations that are less familiar, although no less biologically relevant, such as the G-quadruplex. G-quadruplexes continue to be the subject of a rapidly expanding area of research, owing to their significant potential as therapeutic targets and their unique biophysical properties. This review begins by focusing on G-quadruplex structure, elucidating the intermolecular and intramolecular interactions underlying its formation and highlighting several substructural variants. A variety of methods used to characterize these structures are also outlined. The current state of G-quadruplex research is then addressed by proffering seven pertinent questions for discussion. This review concludes with an overview of possible directions for future research trajectories in this exciting and relevant field. [ABSTRACT FROM AUTHOR]

The concurrent control group is the most important reference for the interpretation of toxicity studies. However, pooled information on control animals from independent studies, i.e., historical control data (HCD), is also used for the interpretation of results. Currently, an overview on actual HCD use in regulatory toxicology is lacking. Therefore, we evaluated the HCD use of the Joint FAO/WHO Meeting on Pesticide Residues from 2004 to 2021 and compared it with recommendations in regulatory guidelines and in the literature. We found that HCD was used routinely and exclusively to avoid potential false positive decisions regarding the treatment-relatedness of effects, mostly using the HCD range, i.e., the most extreme values, as a benchmark. HCD were not used to avoid potential false negative decisions or for quality control of the index study. The central assumption of the HCD use, namely that the HCD and control group of the index study follow the same underlying distribution because they are samples of the same data generation process, was not investigated, although numerous factors potentially contribute to effect variation between the different control groups pooled in the HCD. We recommend that the existing guidelines be revised to improve the robustness and transparency of toxicological assessments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Unmanned aerial spraying systems (UASS), i.e., unmanned aerial vehicles designed for pesticide applications, are widely used in East Asia and increasingly prevalent in other regions of the world, including North America and Europe. However, according to a recent report of the Organization for Economic Co-operation and Development, spray drift and exposure caused by these systems are not yet fully understood. In particular, there are at present no peer-reviewed reports on direct exposure of residents and bystanders to spray drift following UASS applications. This lack of data results in regulatory concerns with respect to the environment and human safety. The objective of this study was to quantify environmental, resident and bystander exposure following the application of a plant protection product to an orchard using a commercial UASS under field conditions. Using a fluorescent tracer, horizontal and vertical downwind drift data were collected and direct exposure of residents and bystanders located downwind the sprayed area to spray drift was quantified using display mannequins equipped with personal air sampling pumps. Spray drift and exposure inversely correlated with sampling height and downwind distance. Furthermore, drift and exposure were strongly influenced by wind speed and direction, albeit hardly affected by the growth stage of the trees. In addition, substantially less tracer was extracted from the filters of the air sampling pumps than from the coveralls worn by mannequins, suggesting that direct resident/bystander exposure to spray drift may predominantly occur via the dermal route. This report provides essential data on UASS spray drift potential that are relevant for environmental and health risk assessments related to these systems. The results are compared to predicted values of current regulatory models and previously reported field data on drift and exposure caused by different spraying equipment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Designed armadillo repeat proteins (dArmRPs) were developed to create a modular peptide binding technology where each of the structural repeats binds two residues of the target peptide. An essential prerequisite for such a technology is a dArmRP geometry that matches the peptide bond length. To this end, we determined a large set (n=27) of dArmRP X-ray structures, of which 12 were previously unpublished, to calculate curvature parameters that define their geometry. Our analysis shows that consensus dArmRPs exhibit curvatures close to the optimal range for modular peptide recognition. Binding of peptide ligands can induce a curvature within the desired range, as confirmed by single-molecule FRET experiments in solution. On the other hand, computationally designed ArmRPs, where side chains have been chosen with the intention to optimally fit into a geometrically optimized backbone, turned out to be more divergent in reality, and thus not suitable for continuous peptide binding. Furthermore, we show that the formation of a crystal lattice can induce small but significant deviations from the curvature adopted in solution, which can interfere with the evaluation of repeat protein scaffolds when high accuracy is required. This study corroborates the suitability of consensus dArmRPs as a scaffold for the development of modular peptide binders., (Copyright © 2017 Elsevier Inc. All rights reserved.)