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7. Sequential development of several RT‐qPCR tests using LNA nucleotides and dual probe technology to differentiate SARS‐CoV‐2 from influenza A and B

Author

Radvánszka, Monika, primary, Paul, Evan D., additional, Hajdu, Roman, additional, Boršová, Kristína, additional, Kováčová, Viera, additional, Putaj, Piotr, additional, Bírová, Stanislava, additional, Čirková, Ivana, additional, Čarnecký, Martin, additional, Buranovská, Katarína, additional, Szobi, Adrián, additional, Vojtaššáková, Nina, additional, Drobná, Diana, additional, Čabanová, Viktória, additional, Sláviková, Monika, additional, Ličková, Martina, additional, Vaňová, Veronika, additional, Fumačová Havlíková, Sabína, additional, Lukáčiková, Ľubomíra, additional, Kajanová, Ivana, additional, Koči, Juraj, additional, Rusňáková, Diana, additional, Sedláčková, Tatiana, additional, Max, Klaas E. A., additional, Tuschl, Thomas, additional, Szemes, Tomáš, additional, Klempa, Boris, additional, and Čekan, Pavol, additional

Published
2022
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10. Cover Image: A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens (Cellular Microbiology 02/2021)

Author

Yang, Xiuli, primary, Koči, Juraj, additional, Smith, Alexis A., additional, Zhuang, Xuran, additional, Sharma, Kavita, additional, Dutta, Shraboni, additional, Rana, Vipin S., additional, Kitsou, Chrysoula, additional, Yas, Ozlem B., additional, Mongodin, Emmanuel F., additional, and Pal, Utpal, additional

Published
2021
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13. A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.

Author

Yang, Xiuli, Koči, Juraj, Smith, Alexis A., Zhuang, Xuran, Sharma, Kavita, Dutta, Shraboni, Rana, Vipin S., Kitsou, Chrysoula, Yas, Ozlem B., Mongodin, Emmanuel F., and Pal, Utpal

Subjects
*LYME disease, *GUT microbiome, *IXODES scapularis, *TICKS, *BORRELIA burgdorferi, *CARRIER proteins, *CHITIN
Abstract

The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen. The structure is thought to play an important role in tick biology. The PM is also known to impact the persistence of tick‐borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance. Herein, we characterise a novel PM‐associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix. The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium. The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti‐PM_CBP antibodies, as revealed by its electron microscopic appearance. Additionally, the duration of tick engorgement on mice and the passage of experimentally‐inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression. The transfer of anti‐PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice. Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick‐borne infections. [ABSTRACT FROM AUTHOR]

Published
2021
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14. Genomic insights into the Ixodes scapularis tick vector of Lyme disease

Author

Gulia-Nuss, Monika, primary, Nuss, Andrew B., additional, Meyer, Jason M., additional, Sonenshine, Daniel E., additional, Roe, R. Michael, additional, Waterhouse, Robert M., additional, Sattelle, David B., additional, de la Fuente, José, additional, Ribeiro, Jose M., additional, Megy, Karine, additional, Thimmapuram, Jyothi, additional, Miller, Jason R., additional, Walenz, Brian P., additional, Koren, Sergey, additional, Hostetler, Jessica B., additional, Thiagarajan, Mathangi, additional, Joardar, Vinita S., additional, Hannick, Linda I., additional, Bidwell, Shelby, additional, Hammond, Martin P., additional, Young, Sarah, additional, Zeng, Qiandong, additional, Abrudan, Jenica L., additional, Almeida, Francisca C., additional, Ayllón, Nieves, additional, Bhide, Ketaki, additional, Bissinger, Brooke W., additional, Bonzon-Kulichenko, Elena, additional, Buckingham, Steven D., additional, Caffrey, Daniel R., additional, Caimano, Melissa J., additional, Croset, Vincent, additional, Driscoll, Timothy, additional, Gilbert, Don, additional, Gillespie, Joseph J., additional, Giraldo-Calderón, Gloria I., additional, Grabowski, Jeffrey M., additional, Jiang, David, additional, Khalil, Sayed M. S., additional, Kim, Donghun, additional, Kocan, Katherine M., additional, Koči, Juraj, additional, Kuhn, Richard J., additional, Kurtti, Timothy J., additional, Lees, Kristin, additional, Lang, Emma G., additional, Kennedy, Ryan C., additional, Kwon, Hyeogsun, additional, Perera, Rushika, additional, Qi, Yumin, additional, Radolf, Justin D., additional, Sakamoto, Joyce M., additional, Sánchez-Gracia, Alejandro, additional, Severo, Maiara S., additional, Silverman, Neal, additional, Šimo, Ladislav, additional, Tojo, Marta, additional, Tornador, Cristian, additional, Van Zee, Janice P., additional, Vázquez, Jesús, additional, Vieira, Filipe G., additional, Villar, Margarita, additional, Wespiser, Adam R., additional, Yang, Yunlong, additional, Zhu, Jiwei, additional, Arensburger, Peter, additional, Pietrantonio, Patricia V., additional, Barker, Stephen C., additional, Shao, Renfu, additional, Zdobnov, Evgeny M., additional, Hauser, Frank, additional, Grimmelikhuijzen, Cornelis J. P., additional, Park, Yoonseong, additional, Rozas, Julio, additional, Benton, Richard, additional, Pedra, Joao H. F., additional, Nelson, David R., additional, Unger, Maria F., additional, Tubio, Jose M. C., additional, Tu, Zhijian, additional, Robertson, Hugh M., additional, Shumway, Martin, additional, Sutton, Granger, additional, Wortman, Jennifer R., additional, Lawson, Daniel, additional, Wikel, Stephen K., additional, Nene, Vishvanath M., additional, Fraser, Claire M., additional, Collins, Frank H., additional, Birren, Bruce, additional, Nelson, Karen E., additional, Caler, Elisabet, additional, and Hill, Catherine A., additional

Published
2016
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23. Candidatus Neoehrlichia mikurensis and its cocirculation with Anaplasma phagocytophilum in Ixodes ricinus ticks across cologically different habitats of Central Europe.

Author

Derdáková, Markéta, Václav, Radovan, Pangrácova-Blanárová, Lucia, Selyemová, Diana, Koči, Juraj, Walder, Gernot, and Špitalská, Eva

Subjects
TICK-borne diseases, ANAPLASMATACEAE, CASTOR bean tick, ANAPLASMA phagocytophilum
Abstract

Background Candidatus Neoehrlichia mikurensis is a newly emerging tick-borne bacterium from the family Anaplasmataceae. Its presence in Ixodes ricinus ticks was reported from various European countries, however, it's ecology and co-circulation with another member of the same family, Anaplasma phagocytophilum has not been rigorously studied yet. Findings Candidatus N. mikurensis was detected in all sampling sites. In total, 4.5% of ticks were positive including larvae. The highest positivity was detected in Austria with a prevalence of 23.5%. The probability of Candidatus N. mikurensis occurrence increased with the proportion of ticks infected with Anaplasma phagocytophilum. Conclusion A positive association between the occurrences of Candidatus N. mikurensis and A. phagocytophilum indicates that both bacteria share similar ecology for their natural foci in Central Europe. [ABSTRACT FROM AUTHOR]

Published
2014
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24. Ultrastructural Changes Caused by Snf7 RNAi in Larval Enterocytes of Western Corn Rootworm (Diabrotica virgifera virgifera Le Conte).

Author

Koči, Juraj, Ramaseshadri, Parthasarathy, Bolognesi, Renata, Segers, Gerrit, Flannagan, Ronald, and Park, Yoonseong

Subjects
*ULTRASTRUCTURE (Biology), *RNA interference, *ENTEROCYTES, *WESTERN corn rootworm, *LARVAE, *PEST control, *ENDOSOMES, *AUTOPHAGY, *UBIQUITINATION
Abstract

The high sensitivity to oral RNA interference (RNAi) of western corn rootworm (WCR, Diabrotica virgifera virgifera Le Conte) provides a novel tool for pest control. Previous studies have shown that RNAi of DvSnf7, an essential cellular component of endosomal sorting complex required for transport (ESCRT), caused deficiencies in protein de-ubiquitination and autophagy, leading to WCR death. Here we investigated the detailed mechanism leading to larval death by analyzing the ultrastructural changes in midgut enterocytes of WCR treated with double-stranded RNA (ds-DvSnf7). The progressive phases of pathological symptoms caused by DvSnf7-RNAi in enterocytes include: 1) the appearance of irregularly shaped macroautophagic complexes consisting of relatively large lysosomes and multi-lamellar bodies, indicative of failure in autolysosome formation; 2) cell sloughing and loss of apical microvilli, and eventually, 3) massive loss of cellular contents indicating loss of membrane integrity. These data suggest that the critical functions of Snf7 in insect midgut cells demonstrated by the ultrastructural changes in DvSnf7 larval enterocytes underlies the conserved essential function of the ESCRT pathway in autophagy and membrane stability in other organisms. [ABSTRACT FROM AUTHOR]

Published
2014
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25. Borrelia afzelii Gene Expression in Ixodes ricinus (Acari: Ixodidae) Ticks

Author

Koči, Juraj, Derdákova, Markéta, Peterková, Kamila, Kazimirová, Mária, Selyemová, Diana, and Labuda, Milan

Abstract

Spirochetes belonging to the Borrelia burgdorferi sensu lato (s. l.) complex have evolved remarkable ability to survive in diverse ecological niches during transmission cycles between ticks and vertebrate hosts by variable gene expression. To understand the events during spirochete transmission from feeding ticks to hosts, mRNA levels of selected B. afzelii genes (bbk32, dbpA, ospA, ospC and vlsE) were measured by quantitative real-time SYBR Green PCR. B. afzelii infected Ixodes ricinus nymphs fed on laboratory BALB/c mice for 0, 24, 48, and 72 hours. The mRNA levels of the constantly expressed flagellin gene were used for the relative quantification of selected genes. Differences in gene expression profiles were observed in unfed ticks and during tick feeding. mRNA levels of bbk32 and dbpA showed distinctive decreasing patterns during the first 24 hours post-attachment, while ospC and vlsE mRNA levels increased significantly during the feeding process. In contrast, ospA levels decreased for the 48 hours of tick feeding and slightly increased by 72 hours. More detailed and comprehensive studies on regulation of gene expression in different Borellia genospecies on the vector–host interface would aid to develop effective strategies in preventing pathogen transmission.

Published
2006
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