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29 results on '"Ko, Jae Hyung"'

5. Effect of flowable thrombin-containing collagen-based hemostatic matrix for preventing pancreatic fistula after pancreatectomy: A randomized clinical trial

Author

PARK, Yejong, primary, KO, Jae Hyung, additional, KANG, Dae Ryong, additional, LEE, Jun Hyeok, additional, HWANG, Dae Wook, additional, LEE, Jae Hoon, additional, LEE, Woohyung, additional, KWON, Jaewoo, additional, PARK, Si-Nae, additional, SONG, Ki-Byung, additional, and KIM, Song Cheol, additional

Published
2021
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7. Effect of Flowable Thrombin-Containing Collagen-Based Hemostatic Matrix for Preventing Pancreatic Fistula after Pancreatectomy: A Randomized Clinical Trial

Author

Park, Yejong, primary, Ko, Jae Hyung, additional, Kang, Dae Ryong, additional, Lee, Jun Hyeok, additional, Hwang, Dae Wook, additional, Lee, Jae Hoon, additional, Lee, Woohyung, additional, Kwon, Jaewoo, additional, Park, Si-Nae, additional, Song, Ki-Byung, additional, and Kim, Song Cheol, additional

Published
2020
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27. Characterization of Flavonoid 7-O-Glucosyltransferase from Arabidopsis thaliana

Author

KIM, Jeong Ho, KIM, Bong Gyu, PARK, Younghee, KO, Jae Hyung, LIM, Chae Eun, LIM, Jun, LIM, Yoongho, and AHN, Joong-Hoon

Abstract

Most flavonoids found in plants exist as glycosides, and glycosylation status has a wide range of effects on flavonoid solubility, stability, and bioavailability. Glycosylation of flavonoids is mediated by Family 1 glycosyltransferases (UGTs), which use UDP-sugars, such as UDP-glucose, as the glycosyl donor. AtGT-2, a UGT from Arabidopsis thaliana, was cloned and expressed in Escherichia colias a gluthatione S-transferase fusion protein. Several compounds, including flavonoids, were tested as potential substrates. HPLC analysis of the reaction products indicated that AtGT-2 transfers a glucose molecule into several different kinds of flavonoids, eriodictyol being the most effective substrate, followed by luteolin, kaempferol, and quercetin. Based on comparison of HPLC retention times with authentic flavonoid 7-O-glucosides and nuclear magnetic resonance spectroscopy, the glycosylation position in the reacted flavonoids was determined to be the C-7 hydroxyl group. These results indicate that AtGT-2encodes a flavonoid 7-O-glucosyltransferase.

Published
2006
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28. Molecular cloning, expression, and characterization of a flavonoid glycosyltransferase from Arabidopsis thaliana

Author

Kim, Jeong Ho, Kim, Bong Gyu, Ko, Jae Hyung, Lee, Youngshim, Hur, Hor-Gil, Lim, Yoongho, and Ahn, Joong-Hoon

Subjects
*MOLECULAR cloning, *FLAVONOIDS, *ARABIDOPSIS thaliana, *PLANT physiology
Abstract

Abstract: Flavonoids are phytochemicals that owe their structural diversity to modifications such as methylation, hydroxylation, and glycosylation. The glycosylation of flavonoids affects solubility, stability and bioavailability and is mediated by the glycosyltransferase (GT) family 1. A GT from Arabidopsis thaliana (AtGT-1) was cloned based on the homology with flavonoid GTs from other plants and classified as UGT73B2 based on the UDP-glycosyltransferase annotation system. Expression of AtGT-1 was detected higher in the flower and leaf than in root but not in the stem. The recombinant AtGT-1 protein expressed in E. coli was tested for activity on several substrates including apigenin, cyanidin, eriodictyol, isorhamnetin, kaempferol, luteolin, naringenin, and quercetin. Flavonols were better substrates for AtGT-1 than flavones or flavanones, and flavanones were better than flavones. Glycosylation of substrates preferentially occurred on the 3-hydroxyl group but the 7-hydroxyl group was glycosylated when the 3-hydroxy group was not available. The structures of kaempferol and naringenin reaction products were determined to be 3-glycosylated kaempferol and 7-glycosylated naringenin, respectively. Kaempferol was the most effective substrate tested. Based on HPLC, LC/MS, and NMR analysis of reaction products, along with the known flavonoid contents of Arabidopsis, AtGT-1 is likely to encode a kaempferol 3-O-UDP glucose transferase. [Copyright &y& Elsevier]

Published
2006
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29. Mannitol production by Leuconostoc citreum KACC 91348P isolated from Kimchi.

Author

Otgonbayar GE, Eom HJ, Kim BS, Ko JH, and Han NS

Subjects
Fermentation, Fructose metabolism, Hydrogen-Ion Concentration, Leuconostoc growth & development, Leuconostoc isolation & purification, Temperature, Food Microbiology, Leuconostoc metabolism, Mannitol metabolism, Vegetables microbiology
Abstract

Leuconostoc genus, which comprise heterofermentative lactic acid bacteria, reduces fructose to mannitol by recycling intracellular NADH. To evaluate the mannitol productivities of different Leuconostoc species, 5 stock cultures and 4 newly isolated strains were cultivated in MRS and simplified media containing glucose and fructose (1:2 ratio). Among them, L. citreum KACC 91348P, which was isolated from kimchi, showed superior result in cell growth rate, mannitol production rate, and yield in both media. The optimal condition for mannitol production of this strain was pH 6.5 and 30°C. When L. citreum KACC was cultured in simplified medium in a 2 l batch fermenter under optimal conditions, the maximum volumetric productivity was 14.83 g·l(-1)h(-1) and overall yield was 86.6%. This strain is a novel and efficient mannitol producer originated from foods to be used for fermentation of fructose-containing foods.

Published
2011
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