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13 results on '"Knitlova, Jarmila"'

3. Adipose-Derived Stem Cells in Reinforced Collagen Gel: A Comparison between Two Approaches to Differentiation towards Smooth Muscle Cells

Author

Filova, Elena, primary, Supova, Monika, additional, Eckhardt, Adam, additional, Vrbacky, Marek, additional, Blanquer, Andreu, additional, Travnickova, Martina, additional, Knitlova, Jarmila, additional, Suchy, Tomas, additional, Ryglova, Sarka, additional, Braun, Martin, additional, Burdikova, Zuzana, additional, Schätz, Martin, additional, Jencova, Vera, additional, Lisnenko, Maxim, additional, Behalek, Lubos, additional, Prochazkova, Renata, additional, Sedlacek, Radek, additional, Kubasova, Kristyna, additional, and Bacakova, Lucie, additional

Published
2023
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8. Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Author

Filova, Elena, primary, Steinerova, Marie, additional, Travnickova, Martina, additional, Knitlova, Jarmila, additional, Musilkova, Jana, additional, Eckhardt, Adam, additional, Hadraba, Daniel, additional, Matejka, Roman, additional, Prazak, Simon, additional, Stepanovska, Jana, additional, Kucerova, Johanka, additional, Riedel, Tomáš, additional, Brynda, Eduard, additional, Lodererova, Alena, additional, Honsova, Eva, additional, Pirk, Jan, additional, Konarik, Miroslav, additional, and Bacakova, Lucie, additional

Published
2021
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10. Minoxidil decreases collagen I deposition and tissue-like contraction in clubfoot-derived cells: a way to improve conservative treatment of relapsed clubfoot?

Author

Knitlova, Jarmila, Doubkova, Martina, Plencner, Martin, Vondrasek, David, Eckhardt, Adam, Ostadal, Martin, Musilkova, Jana, Bacakova, Lucie, and Novotny, Tomas

Subjects
*CONSERVATIVE treatment, *CELL contraction, *MINOXIDIL, *CLUBFOOT, *INHIBITION of cellular proliferation, *FOOT
Abstract

Clubfoot is a congenital deformity affecting the musculoskeletal system, resulting in contracted and stiff tissue in the medial part of the foot. Minoxidil (MXD) has an inhibitory effect on lysyl hydroxylase, which influences the quality of extracellular matrix crosslinking, and could therefore be used to reduce the stiffness and to improve the flexibility of the tissue. We assessed the in vitro antifibrotic effects of minoxidil on clubfoot-derived cells. Cell viability and proliferation were quantified by xCELLigence, MTS, and LIVE/DEAD assays. The amount of collagen I deposited into the extracellular matrix was quantified using immunofluorescence with subsequent image segmentation analysis, hydroxyproline assay, and Second Harmonic Generation imaging. Extracellular matrix contraction was studied in a 3D model of cell-populated collagen gel lattices. MXD concentrations of 0.25, 0.5, and 0.75 mM inhibited the cell proliferation in a concentration-dependent manner without causing a cytotoxic effect. Exposure to ≥0.5 mM MXD resulted in a decrease in collagen type I accumulation after 8 and 21 days in culture. Changes in collagen fiber assembly were observed by immunofluorescence microscopy and nonlinear optical microscopy (second harmonic generation). MXD also inhibited the contraction of cell-populated collagen lattices (0.5 mM by 22%; 0.75 mM by 28%). Minoxidil exerts an in vitro inhibitory effect on the cell proliferation, collagen accumulation, and extracellular matrix contraction processes that are associated with clubfoot fibrosis. This study provides important preliminary results demonstrating the potential relevance of MXD for adjuvant pharmacological therapy in standard treatment of relapsed clubfoot. [ABSTRACT FROM AUTHOR]

Published
2021
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11. Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Author

Eckhardt, Adam, primary, Novotny, Tomas, additional, Doubkova, Martina, additional, Hronkova, Lucia, additional, Vajner, Ludek, additional, Pataridis, Statis, additional, Hadraba, Daniel, additional, Kulhava, Lucie, additional, Plencner, Martin, additional, Knitlova, Jarmila, additional, Liskova, Jana, additional, Uhlik, Jiri, additional, Zaloudikova, Marie, additional, Vondrasek, David, additional, Miksik, Ivan, additional, and Ostadal, Martin, additional

Published
2019
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