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9. Supplementary Figures 1 from Entinostat Enhances the Efficacy of Chemotherapy in Small Cell Lung Cancer Through S-phase Arrest and Decreased Base Excision Repair

Author

Solta, Anna, primary, Boettiger, Kristiina, primary, Kovács, Ildikó, primary, Lang, Christian, primary, Megyesfalvi, Zsolt, primary, Ferk, Franziska, primary, Mišík, Miroslav, primary, Hoetzenecker, Konrad, primary, Aigner, Clemens, primary, Kowol, Christian R., primary, Knasmueller, Siegfried, primary, Grusch, Michael, primary, Szeitz, Beáta, primary, Rezeli, Melinda, primary, Dome, Balazs, primary, and Schelch, Karin, primary

Published
2023
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10. Data from Entinostat Enhances the Efficacy of Chemotherapy in Small Cell Lung Cancer Through S-phase Arrest and Decreased Base Excision Repair

Author

Solta, Anna, primary, Boettiger, Kristiina, primary, Kovács, Ildikó, primary, Lang, Christian, primary, Megyesfalvi, Zsolt, primary, Ferk, Franziska, primary, Mišík, Miroslav, primary, Hoetzenecker, Konrad, primary, Aigner, Clemens, primary, Kowol, Christian R., primary, Knasmueller, Siegfried, primary, Grusch, Michael, primary, Szeitz, Beáta, primary, Rezeli, Melinda, primary, Dome, Balazs, primary, and Schelch, Karin, primary

Published
2023
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11. Supplementary Table 2 from Entinostat Enhances the Efficacy of Chemotherapy in Small Cell Lung Cancer Through S-phase Arrest and Decreased Base Excision Repair

Author

Solta, Anna, primary, Boettiger, Kristiina, primary, Kovács, Ildikó, primary, Lang, Christian, primary, Megyesfalvi, Zsolt, primary, Ferk, Franziska, primary, Mišík, Miroslav, primary, Hoetzenecker, Konrad, primary, Aigner, Clemens, primary, Kowol, Christian R., primary, Knasmueller, Siegfried, primary, Grusch, Michael, primary, Szeitz, Beáta, primary, Rezeli, Melinda, primary, Dome, Balazs, primary, and Schelch, Karin, primary

Published
2023
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12. Supplementary Table 1 from Entinostat Enhances the Efficacy of Chemotherapy in Small Cell Lung Cancer Through S-phase Arrest and Decreased Base Excision Repair

Author

Solta, Anna, primary, Boettiger, Kristiina, primary, Kovács, Ildikó, primary, Lang, Christian, primary, Megyesfalvi, Zsolt, primary, Ferk, Franziska, primary, Mišík, Miroslav, primary, Hoetzenecker, Konrad, primary, Aigner, Clemens, primary, Kowol, Christian R., primary, Knasmueller, Siegfried, primary, Grusch, Michael, primary, Szeitz, Beáta, primary, Rezeli, Melinda, primary, Dome, Balazs, primary, and Schelch, Karin, primary

Published
2023
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13. Entinostat enhances the efficacy of chemotherapy in small cell lung cancer through S-phase arrest and decreased base excision repair

Author

Solta, Anna, primary, Boettiger, Kristiina, additional, Kovács, Ildikó, additional, Lang, Christian, additional, Megyesfalvi, Zsolt, additional, Ferk, Franziska, additional, Mišík, Miroslav, additional, Hoetzenecker, Konrad, additional, Aigner, Clemens, additional, Kowol, Christian R., additional, Knasmueller, Siegfried, additional, Grusch, Michael, additional, Szeitz, Beáta, additional, Rezeli, Melinda, additional, Dome, Balazs, additional, and Schelch, Karin, additional

Published
2023
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14. Impact of mobile phone-specific electromagnetic fields on DNA damage caused by occupationally relevant exposures: results of ex vivo experiments with peripheral blood mononuclear cells from different demographic groups

Author

Mišík, Miroslav, primary, Kundi, Michael, additional, Worel, Nadine, additional, Ferk, Franziska, additional, Hutter, Hans-Peter, additional, Grusch, Michael, additional, Nersesyan, Armen, additional, Herrera Morales, Denise, additional, and Knasmueller, Siegfried, additional

Published
2023
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22. Impact of Bariatric Surgery on the Stability of the Genetic Material, Oxidation, and Repair of DNA and Telomere Lengths

Author

Ferk, Franziska, primary, Mišík, Miroslav, additional, Ernst, Benjamin, additional, Prager, Gerhard, additional, Bichler, Christoph, additional, Mejri, Doris, additional, Gerner, Christopher, additional, Bileck, Andrea, additional, Kundi, Michael, additional, Langie, Sabine, additional, Holzmann, Klaus, additional, and Knasmueller, Siegfried, additional

Published
2023
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23. Use of the single cell gel electrophoresis assay for the detection of DNA-protective dietary factors:Results of human intervention studies

Author

Mišík, Miroslav, Staudinger, Marlen, Kundi, Michael, Worel, Nadine, Nersesyan, Armen, Ferk, Franziska, Dusinska, Maria, Azqueta, Amaya, Møller, Peter, Knasmueller, Siegfried, Mišík, Miroslav, Staudinger, Marlen, Kundi, Michael, Worel, Nadine, Nersesyan, Armen, Ferk, Franziska, Dusinska, Maria, Azqueta, Amaya, Møller, Peter, and Knasmueller, Siegfried

Abstract

The single cell gel electrophoresis technique is based on the measurement of DNA migration in an electric field and enables to investigate via determination of DNA-damage the impact of foods and their constituents on the genetic stability. DNA-damage leads to adverse effects including cancer, neurodegenerative disorders and infertility. In the last 25 years approximately 90 human intervention trials have been published in which DNA-damage, formation of oxidized bases, alterations of the sensitivity towards reactive oxygen species and chemicals and of repair functions were investigated with this technique. In approximately 50% of the studies protective effects were observed. Pronounced protection was found with certain plant foods (spinach, kiwi fruits, onions), coffee, green tea, honey and olive oil. Also diets with increased contents of vegetables caused positive effects. Small amounts of certain phenolics (gallic acid, xanthohumol) prevented oxidative damage of DNA; with antioxidant vitamins and cholecalciferol protective effects were only detected after intake of doses that exceed the recommended daily uptake values. The evaluation of the quality of the studies showed that many have methodological shortcomings (lack of controls, no calibration of repair enzymes, inadequate control of the compliance and statistical analyses) which should be avoided in future investigations., The single cell gel electrophoresis technique is based on the measurement of DNA migration in an electric field and enables to investigate via determination of DNA-damage the impact of foods and their constituents on the genetic stability. DNA-damage leads to adverse effects including cancer, neurodegenerative disorders and infertility. In the last 25 years approximately 90 human intervention trials have been published in which DNA-damage, formation of oxidized bases, alterations of the sensitivity towards reactive oxygen species and chemicals and of repair functions were investigated with this technique. In approximately 50% of the studies protective effects were observed. Pronounced protection was found with certain plant foods (spinach, kiwi fruits, onions), coffee, green tea, honey and olive oil. Also diets with increased contents of vegetables caused positive effects. Small amounts of certain phenolics (gallic acid, xanthohumol) prevented oxidative damage of DNA; with antioxidant vitamins and cholecalciferol protective effects were only detected after intake of doses that exceed the recommended daily uptake values. The evaluation of the quality of the studies showed that many have methodological shortcomings (lack of controls, no calibration of repair enzymes, inadequate control of the compliance and statistical analyses) which should be avoided in future investigations.

Published
2023

24. Measuring DNA modifications with the comet assay:a compendium of protocols

Author

Collins, Andrew, Moller, Peter, Gajski, Goran, Vodenkova, Sona, Abdulwahed, Abdulhadi, Anderson, Diana, Bankoglu, Ezgi Eyluel, Bonassi, Stefano, Boutet-Robinet, Elisa, Brunborg, Gunnar, Chao, Christy, Cooke, Marcus S. S., Costa, Carla, Costa, Solange, Dhawan, Alok, de Lapuente, Joaquin, Del Bo, Cristian, Dubus, Julien, Dusinska, Maria, Duthie, Susan J. J., El Yamani, Naouale, Engelward, Bevin, Gaivao, Isabel, Giovannelli, Lisa, Godschalk, Roger, Guilherme, Sofia, Gutzkow, Kristine B. B., Habas, Khaled, Hernandez, Alba, Herrero, Oscar, Isidori, Marina, Jha, Awadhesh N. N., Knasmueller, Siegfried, Kooter, Ingeborg M. M., Koppen, Gudrun, Kruszewski, Marcin, Ladeira, Carina, Laffon, Blanca, Larramendy, Marcelo, Le Hegarat, Ludovic, Lewies, Angelique, Lewinska, Anna, Liwszyc, Guillermo E. E., de Cerain, Adela Lopez, Manjanatha, Mugimane, Marcos, Ricard, Milic, Mirta, de Andrade, Vanessa Moraes, Moretti, Massimo, Muruzabal, Damian, Novak, Matjaz, Oliveira, Rui, Olsen, Ann-Karin, Owiti, Norah, Pacheco, Mario, Pandey, Alok K. K., Pfuhler, Stefan, Pourrut, Bertrand, Reisinger, Kerstin, Rojas, Emilio, Runden-Pran, Elise, Sanz-Serrano, Julen, Shaposhnikov, Sergey, Sipinen, Ville, Smeets, Karen, Stopper, Helga, Teixeira, Joao Paulo, Valdiglesias, Vanessa, Valverde, Mahara, van Acker, Frederique, van Schooten, Frederik-Jan, Vasquez, Marie, Wentzel, Johannes F. F., Wnuk, Maciej, Wouters, Annelies, Zegura, Bojana, Zikmund, Tomas, Langie, Sabine A. S., Azqueta, Amaya, Collins, Andrew, Moller, Peter, Gajski, Goran, Vodenkova, Sona, Abdulwahed, Abdulhadi, Anderson, Diana, Bankoglu, Ezgi Eyluel, Bonassi, Stefano, Boutet-Robinet, Elisa, Brunborg, Gunnar, Chao, Christy, Cooke, Marcus S. S., Costa, Carla, Costa, Solange, Dhawan, Alok, de Lapuente, Joaquin, Del Bo, Cristian, Dubus, Julien, Dusinska, Maria, Duthie, Susan J. J., El Yamani, Naouale, Engelward, Bevin, Gaivao, Isabel, Giovannelli, Lisa, Godschalk, Roger, Guilherme, Sofia, Gutzkow, Kristine B. B., Habas, Khaled, Hernandez, Alba, Herrero, Oscar, Isidori, Marina, Jha, Awadhesh N. N., Knasmueller, Siegfried, Kooter, Ingeborg M. M., Koppen, Gudrun, Kruszewski, Marcin, Ladeira, Carina, Laffon, Blanca, Larramendy, Marcelo, Le Hegarat, Ludovic, Lewies, Angelique, Lewinska, Anna, Liwszyc, Guillermo E. E., de Cerain, Adela Lopez, Manjanatha, Mugimane, Marcos, Ricard, Milic, Mirta, de Andrade, Vanessa Moraes, Moretti, Massimo, Muruzabal, Damian, Novak, Matjaz, Oliveira, Rui, Olsen, Ann-Karin, Owiti, Norah, Pacheco, Mario, Pandey, Alok K. K., Pfuhler, Stefan, Pourrut, Bertrand, Reisinger, Kerstin, Rojas, Emilio, Runden-Pran, Elise, Sanz-Serrano, Julen, Shaposhnikov, Sergey, Sipinen, Ville, Smeets, Karen, Stopper, Helga, Teixeira, Joao Paulo, Valdiglesias, Vanessa, Valverde, Mahara, van Acker, Frederique, van Schooten, Frederik-Jan, Vasquez, Marie, Wentzel, Johannes F. F., Wnuk, Maciej, Wouters, Annelies, Zegura, Bojana, Zikmund, Tomas, Langie, Sabine A. S., and Azqueta, Amaya

Abstract

The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA–DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility., The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.The comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.

Published
2023

29. Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience: results from the HUMNxl international inter-laboratory scoring exercise

Author

Bolognesi, Claudia, Knasmueller, Siegfried, Nersesyan, Armen, Roggieri, Paola, Ceppi, Marcello, Bruzzone, Marco, Blaszczyk, Ewa, Mielzynska-Svach, Danuta, Milic, Mirta, Bonassi, Stefano, Benedetti, Danieli, Da Silva, Juliana, Toledo, Raphael, Salvadori, Daisy Maria Fávero, Groot de Restrepo, Helena, Filipic, Metka, Hercog, Klara, Aktaş, Ayça, Burgaz, Sema, Kundi, Michael, Grummt, Tamara, Thomas, Philip, Hor, Maryam, Escudero-Fung, Maria, Holland, Nina, and Fenech, Michael

Published
2017
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45. “Micronuclei and Disease” special issue: Aims, scope, and synthesis of outcomes

Author

Fenech, Michael, primary, Knasmueller, Siegfried, additional, Knudsen, Lisbeth E., additional, Kirsch-Volders, Micheline, additional, Deo, Permal, additional, Franzke, Bernhard, additional, Stopper, Helga, additional, Andreassi, Maria-Grazia, additional, Bolognesi, Claudia, additional, Dhillon, Varinderpal S., additional, Laffon, Blanca, additional, Wagner, Karl-Heinz, additional, and Bonassi, Stefano, additional

Published
2021
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46. Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7)

Author

Bonassi, Stefano, Ceppi,Marcello, Møller, Peter, Azqueta, Amaya, Milić, Mirta, Neri, Monica, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine A. S., Teixeira, João Paulo, Bruzzone, Marco, Da Silva, Juliana, Benedetti, Danieli, Cavallo, Delia, Ursini, Cinzia Lucia, Giovannelli, Lisa, Moretti, Silvia, Riso, Patrizia, Del Bo’, Cristian, Russo, Patrizia, Dobrzyńska, Malgorzata, Goroshinskaya, Irina A., Surikova, Ekaterina I., Staruchova, Marta, Barančokova, Magdalena, Volkovova, Katarina, Kažimirova, Alena, Smolkova, Bozena, Laffon, Blanca, Valdiglesias, Vanessa, Pastor, Susana, Marcos, Ricard, Hernández, Alba, Gajski, Goran, Spremo-Potparević, Biljana, Živković, Lada, Boutet-Robinet, Elisa, Perdry, Hervé, Lebailly, Pierre, Perez, Carlos L., Basaran, Nursen, Nemeth, Zsuzsanna, Safar, Anna, Dusinska, Maria, Collins, Andrew, Anderson, Diana, Andrade, Vanessa, Pereira, Cristiana Costa, Costa, Solange, Gutzkow, Kristine B., Ladeira, Carina, Moretti, Massimo, Costa, Carla, Orlow, Irene, Rojas, Emilio, Pourrut, Bertrand, Kruszewski, Marcin, Knasmueller, Siegfried, Shaposhnikov, Sergey, Žegura, Bojana, and Stopper, Helga

Abstract

Link to the corrected article: [https://farfar.pharmacy.bg.ac.rs/handle/123456789/3944]

Published
2021

47. DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Author

Bonassi, Stefano, Ceppi, Marcello, Møller, Peter, Azqueta, Amaya, Milić, Mirta, Monica, Neri, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine A. S., Teixeira, João Paulo, Bruzzone, Marco, Da Silva, Juliana, Benedetti, Danieli, Cavallo, Delia, Ursini, Cinzia Lucia, Giovannelli, Lisa, Moretti, Silvia, Riso, Patrizia, Del Bo’, Cristian, Russo, Patrizia, Dobrzyńska, Malgorzata, Goroshinskaya, Irina A., Surikova, Ekaterina I., Staruchova, Marta, Barančokova, Magdalena, Volkovova, Katarina, Kažimirova, Alena, Smolkova, Bozena, Laffon, Blanca, Valdiglesias, Vanessa, Pastor, Susana, Marcos, Ricard, Hernández, Alba, Gajski, Goran, Spremo-Potparević, Biljana, Živković, Lada, Boutet-Robinet, Elisa, Perdry, Hervé, Lebailly, Pierre, Perez, Carlos L., Basaran, Nursen, Nemeth, Zsuzsanna, Safar, Anna, Dusinska, Maria, Collins, Andrew, Anderson, Diana, Andrade, Vanessa, Pereira, Cristiana Costa, Costa, Solange, Gutzkow, Kristine B., Ladeira, Carina, Moretti, Massimo, Costa, Carla, Orlow, Irene, Rojas, Emilio, Pourrut, Bertrand, Kruszewski, Marcin, Knasmueller, Siegfried, Shaposhnikov, Sergey, Žegura, Bojana, Stopper, Helga, LESUR, Hélène, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS San Raffaele Pisana), Ospedale Policlinico San Martino [Genoa], University of Copenhagen = Københavns Universitet (UCPH), Universidad de Navarra [Pamplona] (UNAV), Instituto de Investigación Sanitaria de Navarra [Pamplona, Spain] (IdiSNA), Institute for Medical Research and Occupational Health, Norwegian Institute of Public Health [Oslo] (NIPH), School of Nutrition and Translational Research in Metabolism [Maastricht] (NUTRIM), Maastricht University [Maastricht], Flemish Institute for Technological Research (VITO), Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Universidade Luterana do Brasil - ULBRA (BRAZIL), Universidade Luterana do Brasil (ULBRA), Istituto Nazionale per l’Assicurazione contro gli Infortuni sul Lavoro [Italian Workers Compensation Authority] (INAIL), NEUROFARBA Department [Firenze, Italy], Università degli Studi di Firenze = University of Florence (UniFI), Université de Florence, Università degli Studi di Milano = University of Milan (UNIMI), IRCCS San Raffaele Scientific Institute [Milan, Italie], National Institute of Public Health - National Institute of Hygiene [Poland], N.N. Blokhin National Medical Research Center of Oncology, Slovak Medical University of Bratislava (SMU), Slovak Academy of Science [Bratislava] (SAS), University of A Coruña (UDC), Instituto de Investigación Biomédica de A Coruña [La Corogne, Espagne] (INIBIC), A Coruña University Hospital [La Corogne, Espagne], Universitat Autònoma de Barcelona (UAB), CIBER de Epidemiología y Salud Pública (CIBERESP), School of Medecine [Belgrade], University of Belgrade [Belgrade], Contaminants & Stress Cellulaire (ToxAlim-COMICS), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Unité de recherche interdisciplinaire pour la prévention et le traitement des cancers (ANTICIPE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM), Instituto de Ciencias Básicas y Preclínicas Victoria de Girón, Hacettepe University = Hacettepe Üniversitesi, National center for public health [Hungary], Norwegian Institute for Air Research (NILU), University of Oslo (UiO), This article is based upon work from COST Action CA15132 (hCOMET), supported by COST (European Cooperation in Science and Technology). The work of SB and PR was supported by funds granted by the Italian Ministry of Health for Institutional Research (Ricerca Corrente)., Farmacologie en Toxicologie, RS: NUTRIM - R3 - Respiratory & Age-related Health, University of Copenhagen = Københavns Universitet (KU), Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Università degli Studi di Milano [Milano] (UNIMI), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER, and Instituto de Saúde Pública da Universidade do Porto

Subjects
Oncology, [SDV]Life Sciences [q-bio], Chromosomal aberrations, Human cancer, Lymphocytes, Predicts, Frequency, Validation, Biomarkers, Hallmarks, Adducts, Repair, Cohort, Kaplan-Meier Estimate, 0302 clinical medicine, Neoplasms, Leukocytes, Medicine, Gel electrophoresis, 0303 health sciences, Multidisciplinary, Hazard ratio, 3. Good health, [SDV] Life Sciences [q-bio], 030220 oncology & carcinogenesis, Biomarker (medicine), LYMPHOCYTES PREDICTS, Comet Assay, Cell-Free Nucleic Acids, medicine.medical_specialty, DNA damage, Science, FREQUENCY, Article, VALIDATION, 03 medical and health sciences, ADDUCTS, Internal medicine, Humans, COHORT, Risk factor, Author Correction, 030304 developmental biology, Proportional Hazards Models, HALLMARKS, REPAIR, HUMAN CANCER, business.industry, Proportional hazards model, Comet assay, biomarker, leukocytes, epidemiology, Comet assay, CHROMOSOMAL-ABERRATIONS, Risk factors, Genotoxicidade Ambiental, business, DNA Damage
Abstract

Author Correction: https://doi.org/10.1038/s41598-021-98620-6. The original version of this Article contained errors: The spelling of the author Monica Neri was incorrectly given as Neri Monica; Additionally, Affiliation 39 was incorrectly given as ‘National Institute of Health, Lisbon, Portugal’, the correct affiliation is listed below: National Institute of Health Doutor Ricardo Jorge, Porto, Portugal. The original Article has been corrected. [Abstract] The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p

Published
2021

48. “Micronuclei and Disease” special issue:Aims, scope, and synthesis of outcomes

Author

Fenech, Michael, Knasmueller, Siegfried, Knudsen, Lisbeth E., Kirsch-Volders, Micheline, Deo, Permal, Franzke, Bernhard, Stopper, Helga, Andreassi, Maria Grazia, Bolognesi, Claudia, Dhillon, Varinderpal S., Laffon, Blanca, Wagner, Karl Heinz, Bonassi, Stefano, Fenech, Michael, Knasmueller, Siegfried, Knudsen, Lisbeth E., Kirsch-Volders, Micheline, Deo, Permal, Franzke, Bernhard, Stopper, Helga, Andreassi, Maria Grazia, Bolognesi, Claudia, Dhillon, Varinderpal S., Laffon, Blanca, Wagner, Karl Heinz, and Bonassi, Stefano

Abstract

The purpose of the “Micronuclei and Disease” special issue (SI) is to: (i) Determine the level of evidence for association of micronuclei (MN), a biomarker of numerical and structural chromosomal aberrations, with risk of specific diseases in humans; (ii) Define plausible mechanisms that explain association of MN with each disease; (iii) Identify knowledge gaps and research needed to translate MN assays into clinical practice. The “MN and Disease” SI includes 14 papers. The first is a review of mechanisms of MN formation and their consequences in humans. 11 papers are systematic reviews and/or meta-analyses of the association of MN with reproduction, child health, inflammation, auto-immune disease, glycation, metabolic diseases, chronic kidney disease, cardiovascular disease, eleven common cancers, ageing and frailty. The penultimate paper focuses on effect of interventions on MN frequency in the elderly. A road map for translation of MN data into clinical practice is the topic of the final paper. The majority of reviewed studies were case-control studies in which the ratio of mean MN frequency in disease cases relative to controls, i.e. the mean ratio (MR), was calculated. The mean of these MR values, estimated by meta-analyses, for lymphocyte and buccal cell MN in non-cancer diseases were 2.3 and 3.6 respectively, and for cancers they were 1.7 and 2.6 respectively. The highest MR values were observed in studies of cancer cases in which MN were measured in the same tissue as the tumour (MR = 4.9–10.8). This special issue is an important milestone in the evidence supporting MN as a reliable genomic biomarker of developmental and degenerative disease risk. These advances, together with results from prospective cohort studies, are helping to identify diseases in which MN assays can be practically employed in the clinical setting to better identify high risk patients and to prioritise them for preventive therapy.

Published
2021

49. “Micronuclei and Disease” special issue: Aims, scope, and synthesis of outcomes

Author

Fenech, Michael, Knasmueller, Siegfried, Knudsen, Lisbeth E., Kirsch-Volders, Micheline, Deo, Permal, Franzke, Bernhard, Stopper, Helga, Andreassi, Maria-Grazia, Bolognesi, Claudia, Dhillon, Varinderpal S., Laffon, Blanca, Wagner, Karl-Heinz, Bonassi, Stefano, Fenech, Michael, Knasmueller, Siegfried, Knudsen, Lisbeth E., Kirsch-Volders, Micheline, Deo, Permal, Franzke, Bernhard, Stopper, Helga, Andreassi, Maria-Grazia, Bolognesi, Claudia, Dhillon, Varinderpal S., Laffon, Blanca, Wagner, Karl-Heinz, and Bonassi, Stefano

Abstract

[Abastract] The purpose of the “Micronuclei and Disease” special issue (SI) is to: (i) Determine the level of evidence for association of micronuclei (MN), a biomarker of numerical and structural chromosomal aberrations, with risk of specific diseases in humans; (ii) Define plausible mechanisms that explain association of MN with each disease; (iii) Identify knowledge gaps and research needed to translate MN assays into clinical practice. The “MN and Disease” SI includes 14 papers. The first is a review of mechanisms of MN formation and their consequences in humans. 11 papers are systematic reviews and/or meta-analyses of the association of MN with reproduction, child health, inflammation, auto-immune disease, glycation, metabolic diseases, chronic kidney disease, cardiovascular disease, eleven common cancers, ageing and frailty. The penultimate paper focuses on effect of interventions on MN frequency in the elderly. A road map for translation of MN data into clinical practice is the topic of the final paper. The majority of reviewed studies were case-control studies in which the ratio of mean MN frequency in disease cases relative to controls, i.e. the mean ratio (MR), was calculated. The mean of these MR values, estimated by meta-analyses, for lymphocyte and buccal cell MN in non-cancer diseases were 2.3 and 3.6 respectively, and for cancers they were 1.7 and 2.6 respectively. The highest MR values were observed in studies of cancer cases in which MN were measured in the same tissue as the tumour (MR = 4.9–10.8). This special issue is an important milestone in the evidence supporting MN as a reliable genomic biomarker of developmental and degenerative disease risk. These advances, together with results from prospective cohort studies, are helping to identify diseases in which MN assays can be practically employed in the clinical setting to better identify high risk patients and to prioritise them for preventive therapy.

Published
2021

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