1. ß-galactosidase from Penicillium canescens. Properties and immobilization
- Author
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Gervydas Dienys, Natalija Gorochovceva, Saulute Budriene, Asta Zubriene, Aldona Miezeliene, Tatjana Romaskevic, and Lyubov V. Yugova
- Subjects
Lactose hydrolysis ,Polyurethane foams ,General Chemistry ,Chitosan ,chemistry.chemical_compound ,Hydrolysis ,chemistry ,Penicillium canescens ,Biochemistry ,Kluyveromyces-fragilis ,Yield (chemistry) ,Materials Chemistry ,Bed ,Lactose ,Polyurethane ,Nuclear chemistry - Abstract
ß-galactosidase from Penicillium canescens was immobilized on chitosan, sepharose-4B, foamable polyurethane and some other carriers. The highest yield of immobilization (up to 98 %) was obtained by using chitosan as a carrier. The optimum pH and temperature were not significantly altered by immobilization. High stability of immobilized ß-galactosidase during storage was demonstrated. Efficient lactose saccharification (over 90 %) in whey was achieved by using immobilized ß-galactosidase. (C) Central European Science Journals. All rights reserved.
- Published
- 2005