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Introduction: Due to the content of nutrients and taste attributes, the plant sprouts could become a valuable component of a healthy diet.

Introduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields.

Introduction: Hop (Humulus lupulus L.) is a common plant in Europe, with many beneficial health effects. In addition to the use in brewing, hops are a valuable source of active substances used in conventional and folk medicine, such as humulones and lupulones, as well as antioxidants, including phenolic compounds.

Introduction: Quince (Cydonia oblonga Mill.) is a plant of which both the fruits and the leaves are sources of compounds with antioxidant potential. Such activity could be helpful to prevent the development of so-called oxidative stress.

Introduction: Antioxidants, isolated from different plant parts, are widely used due to their ability to prevent the development of so-called oxidative stress. Sea buckthorn (Hippophae rhamnoides L.) is one of the plants with expected antioxidant properties.

Summary: Background: Insulin resistance (IR) is an important risk factor for multiple chronic diseases, increasing mortality and reducing life expectancy. The associations between emerging surrogates for IR, triglyceride-glucose index (TyG) and TyG-related indicators, with all-cause mortality and life expectancy in middle-aged and older patients in primary care are unclear. Methods: This study originated from the Polish primary care cohort LIPIDOGRAM2015, including patients aged ≥45 years. Baseline fasting triglycerides and fasting glucose were used to derive TyG. Other TyG-related indicators included TyG-adjusted body mass index (TyG-BMI), TyG-adjusted waist circumference (TyG-WC), TyG-adjusted waist-to-hip, and TyG-adjusted waist-to-height. In this longitudinal analysis, we assessed associations between TyG-related indicators with total all-cause mortality, premature (age at death ≤75 years) all-cause mortality and years of life lost (YLL). Findings: We included 10,688 patients (mean age 61.8 ± 9.3 years; 63.5% female). Cumulative total and premature all-cause mortality were 7.2% and 4.6%, respectively, during 5.7 years (IQR 5.6–5.7) of follow-up. Lowest (Q1) and highest quartile (Q4) of TyG-BMI and TyG-WC were associated with total all-cause mortality (second quartile [Q2]: reference; TyG-BMI: Q1: aHR 1.33, 95% CI 1.07–1.65, Q4: aHR 1.28, 95% CI 1.03–1.58; TyG-WC: Q1: aHR 1.44, 95% CI 1.14–1.82, Q4: aHR 1.29, 95% CI 1.04–1.59), similar results for premature all-cause mortality. Within age 45–80 years, compared with Q2 and third quartile, YLL were 4.49 and 5.46 years for TyG-BMI Q1 and Q4, respectively, 3.24 and 5.31 years for TyG-WC Q1 and Q4, respectively. Interpretation: TyG-BMI and TyG-WC demonstrated a U-shaped association with total and premature all-cause mortality. Low and high levels of TyG-BMI and TyG-WC were associated with reduced life expectancy. Despite the relatively short follow-up period, significant associations were still observed, but longer follow-up studies are required to further explore these relationships. Funding: Polish Lipid Association, College of Family Physician in Poland, Valeant in Poland.

The purpose of this article is to present the policy toward Jewish youth carried out by the main Jewish institutions operating in Lublin in the postwar period (until 1968), including the local representation of the Central Committee of Polish Jews (CKŻP) (1945–1950) and the Lublin branch of the Social and Cultural Society of Jews in Poland (TSKŻ), as well as the activities of local Zionist youth organizations. The paper presents the organizational structures of the youth-specialized units operating within the aforementioned institutions, the formal and personal changes within them, as well as the local particularities of their activity. The ideological assumptions of these organizations are analyzed and set in the context of the cultural debate over the possibility of rebuilding Jewish life in Poland: nusekh Poyln and yetsiyes Poyln.

IntroductionSanguisorba officinalis L. is classified as a medicinal plant and used in traditional medicine. The root of this plant is mainly used as a medicinal raw material, but the above-ground parts are also a valuable source of health-promoting biologically active compounds.MethodThe study aimed to evaluate the antioxidant activity and total polyphenol content (TPC) of extracts prepared in 70% and 40% aqueous ethanol solution (dry extract content 50–500 g/L) from the aerial parts of S. officinalis. The essential oil was isolated from the tested raw material, and its composition was determined using GC-MS. Ethanolic extracts and essential oil have been tested for antibacterial activity. The extract in 70% v/v ethanol (dry extract content: 500 g/L) was subjected to HPLC analysis for the content of selected phenolic acids and an ex vivo skin permeation study. The ability of these metabolites to permeate and accumulate in the skin was analysed.ResultsExtracts prepared at both ethanol concentrations showed similar antioxidant activity and TPC. Depending on the method, concentration of solvent, and dry extract content (50–500 g/L), the activity ranged from 1.97 to 84.54 g Trolox/L. TPC range of 3.80–37.04 g GA/L. Gallic acid (424 mg/L) and vanillic acid (270 mg/L) had the highest concentrations among the phenolic acids analysed. Vanillic acid (10 μg) permeates the skin at the highest concentration. The highest accumulation in the skin was found for 2,5-dihydroxybenzoic acid (53 μg/g skin), 2,3-dihydroxybenzoic acid (45 μg/g skin), and gallic acid (45 μg/g skin). The tested ethanolic extracts exhibited antibacterial activity. Samples with a dry extract concentration of 500 g/L showed the largest growth inhibition zones. The most sensitive strains to these extracts were P. aeruginosa (24 mm), S. lutea (23 mm), and S. pneumoniae (22 mm). The smallest inhibition zones were observed for B. subtilis (17 mm). The essential oil showed weaker antimicrobial activity (growth inhibition zone 8–10 mm). The GC-MS method identified 22 major components of the essential oil, including aliphatic hydrocarbons, unsaturated terpene alcohols, aliphatic aldehydes, unsaturated and saturated fatty acids, sesquiterpene, phytyl ester of linoleic acid, nitrogen compound, phytosterol, terpene ketone, phenylpropanoids, aliphatic alcohol, diterpenoid, aromatic aldehyde, and aliphatic carboxylic acid.DiscussionThe conducted research has shown that ethanolic extracts from Sanguisorbae herba are a valuable source of compounds with antibacterial and antioxidant potential, including phenolic acids. The fact that selected phenolic acids contained in the tested extract have the ability to permeate and accumulate in the skin provides the basis for conducting extended research on the use of extracts from this plant raw material in cosmetic and pharmaceutical preparations applied to the skin.

The paper presents an application of the ASMD-FSMD technique for designing high-performance digital circuits on the example of an implementation of sequential multipliers in reconfigurable FPGA devices. The method primarily enables multiple operations on the same variable within a single clock cycle. The experiments were conducted using the QuartusPrime tool and Cyclone 10 LP devices, as well as Vivado tools and the Kintex UltraScale family device. The bit size of multiplicands varied from 4 to 128. A comparison of the ASMD-FSMD technique with the traditional approach using datapath with the controller has shown that the performance of the sequential multipliers increases by a factor of 2 and, for some examples, by a factor of 3. Practical recommendations for using the ASMD-FSMD technique to improve the performance of digital devices, as well as directions for further studies, are given in the conclusion.

Substances that delay the skin aging process have become very popular lately. Undoubtedly, this is influenced by all kinds of efforts to maintain a youthful appearance for as long as possible. Plant-derived antioxidants are a group of compounds that exhibit protective properties against the degenerative effects of oxidative stress on skin aging. Another important factor that protects skin against aging is photoprotective agents. The comparison of antioxidant and photoprotective activities seems to be interesting. The aim of this study was to evaluate the antioxidant properties of Pinus strobus, Pinus nigra, and Pinus mugo extracts using two frequently applied methods, i.e., DPPH and ABTS. Moreover, the polyphenol content was evaluated using Folin–Ciocalteu method. The correlation between the polyphenol content, antioxidant potential of the extracts, and sun protection factor in vitro was evaluated. Extracts were prepared using methanol, ethanol, isopropanol, and n-propanol in three concentrations: 40% (v/v), 70% (v/v), and undiluted. Ultrasound-assisted extraction, which is a type of green extraction technique, was applied for 15, 30, or 60 min. The highest antioxidant activity determined by the DPPH and ABTS methods was observed for Pinus mugo extracts in 40% ethanol and 40% methanol, respectively, both after 15 min extraction. The highest total polyphenol content was also found in Pinus mugo extracts. These activities were significantly higher than those of Pinus strobus and Pinus nigra. Similarly, the highest SPF values were also found for Pinus mugo extracts. Moreover, a strong correlation was observed between the antioxidant potential and SPF—the highest values were found for the correlation between the SPF and antioxidant activity determined using the ABTS method. Based on the obtained results, Pinus mugo could be suggested as a possible component for use in cosmetics.

ABSTRACT The Gardnerella genus, comprising at least 13 species, is associated with the polymicrobial disorder bacterial vaginosis (BV). However, the details of BV pathogenesis are poorly defined, and the contributions made by individual species, including Gardnerella spp., are largely unknown. We report here that colony phenotypes characterized by size (large and small) and opacity (opaque and translucent) are phase variable and are conserved among all tested Gardnerella strains, representing at least 10 different species. With the hypothesis that these different variants could be an important missing piece to the enigma of how BV develops in vivo, we characterized their phenotypic, proteomic, and genomic differences. Beyond increased colony size, large colony variants showed reduced vaginolysin secretion and faster growth rate relative to small colony variants. The ability to inhibit the growth of Neisseria gonorrhoeae and commensal Lactobacillus species varied by strain and, in some instances, differed between variants. Proteomics analyses indicated that 127–173 proteins were differentially expressed between variants. Proteins with increased expression in large variants of both strains were associated with amino acid and protein synthesis and protein folding, whereas those increased in small variants were related to nucleotide synthesis, phosphate transport, ABC transport, and glycogen breakdown. Furthermore, whole genome sequencing analyses revealed an abundance of genes associated with variable homopolymer tracts, implicating slipped strand mispairing in Gardnerella phase variation and illuminating the potential for previously unrecognized heterogeneity within clonal populations. Collectively, these results suggest that phase variants may be primed to serve different roles in BV pathogenesis.IMPORTANCEBacterial vaginosis is the most common gynecological disorder in women of childbearing age. Gardnerella species are crucial to the development of this dysbiosis, but the mechanisms involved in the infection are not understood. We discovered that Gardnerella species vary between two different forms, reflected in bacterial colony size. A slow-growing form makes large amounts of the toxin vaginolysin and is better able to survive in human cervix tissue. A fast-growing form is likely the one that proliferates to high numbers just prior to symptom onset and forms the biofilm that serves as a scaffold for multiple BV-associated anaerobic bacteria. Identification of the proteins that vary between different forms of the bacteria as well as those that vary randomly provides insight into the factors important for Gardnerella infection and immune avoidance.

European society is aging, and it is becoming visible than ever before. The Old World is not only a name identified with the cradle of the Western civilisation, but also a symbol of demographic changes, which are one of the main challenges for local authorities. The aging of the European society is a process that has a significant impact on the local policy. In many European cities and municipalities, actions taken by local authorities have become an example of finding solutions that will be an adequate response to this process. The subject of the analysis in this article is membership of European cities and municipalities in the network of the World Health Organization (WHO) as a phenomenon expressing the activity of local authorities towards the process of aging residents. Membership in the Global Network of Age-friendly Cities and Communities can be treated, on the one hand, as one of the forms of support in these activities. It brings together cities and communities that meet certain conditions regarding actions taken for the benefit of the elderly people. On the one hand, membership is a form of recognition by WHO of the activities of local authorities. On the other hand, it is a platform for exchanging experiences and good practices. European cities and municipalities constitute a significant part of this network. The main research problem addressed in this article concerns the membership cities and communities from Europe in the Global Network of Age-friendly Cities and Communities. The development of the network is an expression of the importance and legitimacy of the age-friendly concept and the participation of European cities and communities in the network indicates its importance in Europe. This analysis is aimed on determining the representativeness of European cities and municipalities in the network, including the division into individual European countries.

Abstract Background Resistance to apoptosis is a hallmark of cancer and proteins regulating apoptosis have been proposed as prognostic markers in several malignancies. However, the prognostic impact of apoptotic markers has not been consistently demonstrated in oral squamous cell carcinoma (OSCC). This inconsistency in reported associations between apoptotic proteins and prognosis can be partly attributed to the intrinsic low resolution and misclassification associated with manual, semi-quantitative methods of biomarker expression measurement. The aim of this study was to examine the association between apoptosis-regulating proteins and clinical outcomes in oral squamous cell carcinoma (OSCC) using the quantitative fluorescence immunohistochemistry (IHC) based AQUAnalysis technique. Methods Sixty-nine OSCC patients diagnosed between 1998–2005 in Calgary, Alberta, Canada were included in the study. Clinical data were obtained from the Alberta Cancer Registry and chart review. Tissue microarrays (TMAs) were assembled from triplicate cores of formalin-fixed paraffin embedded pre-treatment tumour tissue. Bax, Bcl-2 and Bcl-XL protein expression was quantified using fluorescent IHC and AQUA technology in normal oral cavity squamous epithelium (OCSE) and OSCC tumour samples. Survival was analyzed using Kaplan-Meier plots and the Cox proportional hazard model. Results Bax expression was predominantly nuclear in OCSE and almost exclusively cytoplasmic in OSCC. No similar differences in localization were observed for Bcl-2 or Bcl-XL. Only Bax expression associated with disease-specific survival (DSS), with 5-year survival estimates of 85.7% for high Bax versus 50.3% for low Bax (p = 0.006), in univariate analysis. High Bax expression was also significantly associated with elevated Ki67 expression, indicating that increased proliferation might lead to an improved response to radiotherapy in patients with elevated Bax expression. In multivariate analyses, Bax protein expression remained an independent predictor of DSS in OSCC [HR 0.241 (0.078-0.745), p = 0.013]. Conclusions The AQUA technique used in our study eliminates observer bias and provides reliable and reproducible estimates for biomarker expression. AQUA also provides essential measures of quality control that cannot be achieved with manual biomarker scoring techniques. Our results support the use of Bax protein expression as a prognostic marker in conjunction with other clinico-pathological variables when designing personalized treatment strategies for OSCC patients.

IgE induced by type 2 immune responses in atopic dermatitis is implicated in the progression of atopic dermatitis to other allergic diseases, including food allergies, allergic rhinitis, and asthma. However, the keratinocyte-derived signals that promote IgE and ensuing allergic diseases remain unclear. Herein, in a mouse model of atopic dermatitis-like skin inflammation induced by epicutaneous Staphylococcus aureus exposure, keratinocyte release of IL‑36α along with IL-4 triggered B cell IgE class-switching, plasma cell differentiation, and increased serum IgE levels-all of which were abrogated in IL-36R-deficient mice or anti-IL‑36R-blocking antibody-treated mice. Moreover, skin allergen sensitization during S. aureus epicutaneous exposure-induced IL-36 responses was required for the development of allergen-specific lung inflammation. In translating these findings, elevated IL‑36 cytokines in human atopic dermatitis skin and in IL‑36 receptor antagonist-deficiency patients coincided with increased serum IgE levels. Collectively, keratinocyte-initiated IL‑36 responses represent a key mechanism and potential therapeutic target against allergic diseases.

We designed an activity-based science curriculum that used Minecraft to support microbiology learning for students enrolled in the Lang Science Program at the American Museum of Natural History (AMNH) in New York City. Minecraft offered an option to consolidate complex science content into digestible activities for modeling concepts and demonstrate student mastery. We will (1) present a background of the course, design processes, and how we used Minecraft in the curriculum, (2) describe the design of the educational Minecraft activities, (3) articulate design issues, adjustments, and constraints, and (4) discuss future changes.

The effect of the diet modification (soybean and lupine addition) on the content of protein and amino acids (AA) in eggs was studied. Both the sampling day and the diet influenced the total protein content. In albumen, the lowest protein content (10.6%) was noted after administering a diet containing 25% lupine; in the same egg the yolk contained the most proteins (16.7%). In the content of nonessential AA (NAA) in egg yolks, differences were noted only for cysteine, with its the highest content in the yolks of the control group. The stable content of essential yolk amino acids (EAA) was observed only for isoleucine, leucine, tryptophan and phenylalanine. The highest contents of EAA and NAA were recorded in the yolks of the control group (~47 and ~53 g/100 g of protein, respectively) and in the group with 25% additions of lupine (~42 and ~51 g/100 g of protein, respectively). AA with constant content in the tested albumens were methionine, tryptophan and alanine. The highest content of EAA (>~42 g/100 g of protein) and NAA (>~62 g/100 g of protein) were determined in albumen of eggs determined in the group with at least 20% additions of lupine. The highest content of EAA for humans delivered eggs from groups 4–6 (with the addition of soy into the diet ≤5%). The protein sources used in the hen diet significantly influenced the content of protein and individual AA in the produced eggs.

Bovine Respiratory Disease (BRD) poses a significant threat to cattle welfare worldwide, affecting their respiratory system and causing substantial economic losses. BRD is multifactorial in nature. This research aimed to investigate the serological profile of calves for the five main bovine respiratory pathogens. Serum samples were collected from dairy calves aged 7–12 months that had never been vaccinated against tested pathogens and had recently shown signs of BRD. A total of 4095 calves from 650 dairy herds located in south-western Poland were examined. The Commercial Indirect Respiratory ELISA Kit Multiplexed—Double well—BIO K 284/5 (Bio-X Diagnostics, Rochefort, Belgium) was used to determine the presence of antibodies against BVDV, BoAHV1, BRSV, BPIV3, and M. bovis. The presence of antibodies against at least one of the tested pathogens was found in 306 (47.08%) herds. The overall prevalence of antibodies was as follows: BoAHV1 21.54%, BVDV 32.0%, BRSV 34.15%, BPIV3 34.31%, and M. bovis 31.38%. The strongest correlation was between BRSV antibodies positive sera and BPIV3 antibodies positive sera. Among the five pathogens tested, antibodies to BVDV, BRSV, BPIV3, and M. bovis were most commonly detected simultaneously. The results of this study indicate the need for preventive measures against these pathogens in the studied region.

α-Pinene is the bicyclic, unsaturated terpene hydrocarbon present in many plants. Due to its beneficial chemical properties, this compound is of great interest and has found numerous applications as a raw material in many chemical industries as well as in medicine and cosmetics. The aim of this study was to evaluate the antioxidant activities of ethanolic extracts obtained from plants containing α-pinene and to test the properties of cosmetic emulsions prepared with these extracts. The raw plant materials consisted of fresh parts of Pinus sylvestris L., such as cones, needles, and branches, as well as dried unground and ground pinecones; dried and fresh Rosmarinus officinalis leaves; dried Levisticum officinale leaves; and dried Salvia officinalis L. leaves. The plant materials were individually extracted with 40% (v/v), 70% (v/v), and 96% (v/v) ethanol using ultrasound-assisted extraction (UAE) for 15, 30, or 60 min. This method is a green extraction technique, frequently applied to isolate active substances from plants. For the selected plant materials, Soxhlet extraction with 96% (v/v) ethanol was also performed. The qualitative and quantitative analyses of the components in the selected extracts were performed with gas chromatography coupled with mass spectrometry (GC-MS). The antioxidant activities of the extracts were evaluated with the DPPH and ABTS methods. The extracts of three plant materials with the highest antioxidant activities—dried Rosmarinus officinalis leaves, dried Salvia officinalis L. leaves, and dried and ground Pinus sylvestris L. cones—were selected to be incorporated in cosmetic emulsions containing glyceryl monostearate and Olivem 1000 as emulsifiers. The stabilities and antioxidant activities of the emulsions were evaluated. Moreover, the antimicrobial properties of the emulsions using microbiological tests were also determined. The findings suggest that the prepared emulsions are stable cosmetic products with a high antioxidant potential.

The paper presents an application of the ASMD-FSMD technique for designing high-performance digital circuits on the example of an implementation of sequential multipliers in reconfigurable FPGA devices. The method primarily enables multiple operations on the same variable within a single clock cycle. The experiments were conducted using the QuartusPrime tool and Cyclone 10 LP devices, as well as Vivado tools and the Kintex UltraScale family device. The bit size of multiplicands varied from 4 to 128. A comparison of the ASMD-FSMD technique with the traditional approach using datapath with the controller has shown that the performance of the sequential multipliers increases by a factor of 2 and, for some examples, by a factor of 3. Practical recommendations for using the ASMD-FSMD technique to improve the performance of digital devices, as well as directions for further studies, are given in the conclusion. [ABSTRACT FROM AUTHOR]

Substances that delay the skin aging process have become very popular lately. Undoubtedly, this is influenced by all kinds of efforts to maintain a youthful appearance for as long as possible. Plant-derived antioxidants are a group of compounds that exhibit protective properties against the degenerative effects of oxidative stress on skin aging. Another important factor that protects skin against aging is photoprotective agents. The comparison of antioxidant and photoprotective activities seems to be interesting. The aim of this study was to evaluate the antioxidant properties of Pinus strobus, Pinus nigra, and Pinus mugo extracts using two frequently applied methods, i.e., DPPH and ABTS. Moreover, the polyphenol content was evaluated using Folin–Ciocalteu method. The correlation between the polyphenol content, antioxidant potential of the extracts, and sun protection factor in vitro was evaluated. Extracts were prepared using methanol, ethanol, isopropanol, and n-propanol in three concentrations: 40% (v/v), 70% (v/v), and undiluted. Ultrasound-assisted extraction, which is a type of green extraction technique, was applied for 15, 30, or 60 min. The highest antioxidant activity determined by the DPPH and ABTS methods was observed for Pinus mugo extracts in 40% ethanol and 40% methanol, respectively, both after 15 min extraction. The highest total polyphenol content was also found in Pinus mugo extracts. These activities were significantly higher than those of Pinus strobus and Pinus nigra. Similarly, the highest SPF values were also found for Pinus mugo extracts. Moreover, a strong correlation was observed between the antioxidant potential and SPF—the highest values were found for the correlation between the SPF and antioxidant activity determined using the ABTS method. Based on the obtained results, Pinus mugo could be suggested as a possible component for use in cosmetics. [ABSTRACT FROM AUTHOR]

Numerous applications of AI are found in the banking sector. Starting from the front-office, enhancing customer recognition and personalized services, continuing in the middle-office with automated fraud-detection systems, ending with the back-office and internal processes automatization. In this paper we provide comprehensive information on the phenomenon of peer-to-peer lending in the modern view of alternative finance and crowdfunding from several perspectives. The aim of this research is to explore the phenomenon of peer-to-peer lending market model. We apply and check the suitability and effectiveness of credit scorecards in the marketplace lending along with determining the appropriate cut-off point. We conducted this research by exploring recent studies and open-source data on marketplace lending. The scorecard development is based on the P2P loans open dataset that contains repayments record along with both hard and soft features of each loan. The quantitative part consists in applying a machine learning algorithm in building a credit scorecard, namely logistic regression.

BACKGROUND:The majority of patients with pancreatic cancer who undergo curative resection experience rapid disease recurrence. In previous small studies, high expression of the mismatch-repair protein mutL protein homolog 1 (MLH1) in pancreatic cancers was associated with better outcomes. The objective of this study was to validate the association between MLH1 expression and survival in patients who underwent resection of pancreatic cancer and received adjuvant chemoradiation. METHODS:Samples were obtained from the NRG Oncology Radiation Therapy Oncology Group 9704 prospective, randomized trial (clinicaltrials.gov identifier NCT00003216), which compared 2 adjuvant protocols in patients with pancreatic cancer who underwent resection. Tissue microarrays were prepared from formalin-fixed, paraffin-embedded, resected tumor tissues. MLH1 expression was quantified using fluorescence immunohistochemistry and automated quantitative analysis, and expression was dichotomized above and below the median value. RESULTS:Immunohistochemical staining was successfully performed on 117 patients for MLH1 (60 and 57 patients from the 2 arms). The characteristics of the participants who had tissue samples available were similar to those of the trial population as a whole. At the time of analysis, 84% of participants had died, with a median survival of 17 months. Elevated MLH1 expression levels in tumor nuclei were significantly correlated with longer disease-free and overall survival in each arm individually and in both arms combined. Two-year overall survival was 16% in patients who had low MLH1 expression levels and 53% in those who had high MLH1 expression levels (P

BACKGROUND: The presence of Major Depressive Disorder (MDD) is often comorbid in patients with a variety of general medical conditions (GMCs) which could lead to less favorable outcomes. OBJECTIVE: The goal of this analysis is to examine functional outcomes of QOL and functioning before and after antidepressant treatment among patients with MDD with and without GMCs. METHODS: We performed a secondary analysis based on the STAR*D database. The analysis included two patient groups from the STAR*D trial: 1,198 patients comorbid with MDD and GMCs (MDD + GMC) and 1,082 patients with MDD and no GMCs (MDDnoGMC), as defined by the Cumulative Illness Rating Scale. We analyzed depressive symptom severity, functioning and quality of life (QOL) before and after level 1 treatment with citalopram. RESULTS: At baseline, the MDD + GMC group had significantly lower QOL (p < 0.001) and functioning (p = 0.001) than the MDDnoGMC group, although depressive symptom severity was not significantly different. Following antidepressant treatment, QOL, functioning and depressive symptom severity significantly improved for both MDD + GMC and MDDnoGMC groups. However, patients with MDD + GMC were more likely to experience severe impairments in QOL in (56.8% vs. 43.5% for MDDnoGMC, p < 0.001) and functioning (42.5% vs. 29.3% for MDDnoGMC, p < 0.001) following treatment. The remission rate was significantly lower for MDD + GMC (30.6% vs. 41.1% for MDDnoGMC, p < 0.001). CONCLUSIONS: Our findings suggest that antidepressant treatment had a positive impact on patients with and without GMCs. However, those with GMCs experienced not only a lower remission rate, but also continued to experience more significantly severe impairments in QOL and functioning.

Abstract Bronchial hyperresponsiveness is a typical, but non-specific feature of cough variant asthma (CVA). This study aimed to determine whether bronchial hyperresponsiveness may be considered as a predictor of CVA in non-smoking adults with chronic cough (CC). The study included 55 patients with CC and bronchial hyperresponsiveness confirmed in the methacholine provocation test, in whom an anti-asthmatic, gradually intensified treatment was introduced. The diagnosis of CVA was established if the improvement in cough severity and cough-related quality of life in LCQ were noted.The study showed a high positive predictive value of bronchial hyperresponsiveness in this population. Cough severity and cough related quality of life were not related to the severity of bronchial hyperresponsiveness in CVA patients. A poor treatment outcome was related to a low baseline capsaicin threshold and the occurrence of gastroesophageal reflux-related symptoms. In conclusion, bronchial hyperresponsiveness could be considered as a predictor of cough variant asthma in non-smoking adults with CC.

Introduction: Sanguisorba officinalis L. is classified as a medicinal plant and used in traditional medicine. The root of this plant is mainly used as a medicinal raw material, but the above-ground parts are also a valuable source of healthpromoting biologically active compounds. Method: The study aimed to evaluate the antioxidant activity and total polyphenol content (TPC) of extracts prepared in 70% and 40% aqueous ethanol solution (dry extract content 50-500 g/L) from the aerial parts of S. officinalis. The essential oil was isolated from the tested raw material, and its composition was determined using GC-MS. Ethanolic extracts and essential oil have been tested for antibacterial activity. The extract in 70% v/v ethanol (dry extract content: 500 g/L) was subjected to HPLC analysis for the content of selected phenolic acids and an ex vivo skin permeation study. The ability of these metabolites to permeate and accumulate in the skin was analysed. Results: Extracts prepared at both ethanol concentrations showed similar antioxidant activity and TPC. Depending on the method, concentration of solvent, and dry extract content (50-500 g/L), the activity ranged from 1.97 to 84.54 g Trolox/L. TPC range of 3.80-37.04 g GA/L. Gallic acid (424 mg/L) and vanillic acid (270 mg/L) had the highest concentrations among the phenolic acids analysed. Vanillic acid (10 µg) permeates the skin at the highest concentration. The highest accumulation in the skin was found for 2,5-dihydroxybenzoic acid (53 μg/g skin), 2,3-dihydroxybenzoic acid (45 µg/g skin), and gallic acid (45 µg/g skin). The tested ethanolic extracts exhibited antibacterial activity. Samples with a dry extract concentration of 500 g/L showed the largest growth inhibition zones. The most sensitive strains to these extracts were P. aeruginosa (24 mm), S. lutea (23 mm), and S. pneumoniae (22 mm). The smallest inhibition zones were observed for B. subtilis (17 mm). The essential oil showed weaker antimicrobial activity (growth inhibition zone 8-10 mm). The GC-MS method identified 22 major components of the essential oil, including aliphatic hydrocarbons, unsaturated terpene alcohols, aliphatic aldehydes, unsaturated and saturated fatty acids, sesquiterpene, phytyl ester of linoleic acid, nitrogen compound, phytosterol, terpene ketone, phenylpropanoids, aliphatic alcohol, diterpenoid, aromatic aldehyde, and aliphatic carboxylic acid. Discussion: The conducted research has shown that ethanolic extracts from Sanguisorbae herba are a valuable source of compounds with antibacterial and antioxidant potential, including phenolic acids. The fact that selected phenolic acids contained in the tested extract have the ability to permeate and accumulate in the skin provides the basis for conducting extended research on the use of extracts from this plant raw material in cosmetic and pharmaceutical preparations applied to the skin. [ABSTRACT FROM AUTHOR]

Simple Summary: Bovine Respiratory Disease (BRD) is a major concern for cattle welfare globally, causing respiratory issues and significant economic losses. This study investigated the presence of antibodies against five BRD pathogens in calves from south-western Poland. Researchers tested serum samples from 4095 dairy calves, aged 7–12 months, that recently showed BRD symptoms and had never been vaccinated. They found that 47.08% of the herds had antibodies against at least one of the tested pathogens. The overall prevalence rates were as follows: BoAHV1 (21.54%), BVDV (32.0%), BRSV (34.15%), BPIV3 (34.31%), and M. bovis (31.38%). Notably, BRSV and BPIV3 antibodies often appeared together in serum samples. This study underscores the importance of preventive measures against these pathogens to protect cattle health and reduce economic losses in the region. Bovine Respiratory Disease (BRD) poses a significant threat to cattle welfare worldwide, affecting their respiratory system and causing substantial economic losses. BRD is multifactorial in nature. This research aimed to investigate the serological profile of calves for the five main bovine respiratory pathogens. Serum samples were collected from dairy calves aged 7–12 months that had never been vaccinated against tested pathogens and had recently shown signs of BRD. A total of 4095 calves from 650 dairy herds located in south-western Poland were examined. The Commercial Indirect Respiratory ELISA Kit Multiplexed—Double well—BIO K 284/5 (Bio-X Diagnostics, Rochefort, Belgium) was used to determine the presence of antibodies against BVDV, BoAHV1, BRSV, BPIV3, and M. bovis. The presence of antibodies against at least one of the tested pathogens was found in 306 (47.08%) herds. The overall prevalence of antibodies was as follows: BoAHV1 21.54%, BVDV 32.0%, BRSV 34.15%, BPIV3 34.31%, and M. bovis 31.38%. The strongest correlation was between BRSV antibodies positive sera and BPIV3 antibodies positive sera. Among the five pathogens tested, antibodies to BVDV, BRSV, BPIV3, and M. bovis were most commonly detected simultaneously. The results of this study indicate the need for preventive measures against these pathogens in the studied region. [ABSTRACT FROM AUTHOR]

Partitioning proteins are well studied as molecular organizers of chromosome and plasmid segregation during division, however little is known about the roles partitioning proteins can play within type IV secretion systems. The single-stranded DNA (ssDNA)-secreting gonococcal T4SS has two partitioning proteins, ParA and ParB. These proteins work in collaboration with the relaxase TraI as essential facilitators of type IV secretion. Bacterial two-hybrid experiments identified interactions between each partitioning protein and the relaxase. Subcellular fractionation demonstrated that ParA is found in the cellular membrane, whereas ParB is primarily in the membrane, but some of the protein is in the soluble fraction. Since TraI is known to be membrane-associated, these data suggest that the gonococcal relaxosome is a membrane-associated complex. In addition, we found that translation of ParA and ParB is controlled by an RNA switch. Different mutations within the stem-loop sequence predicted to alter folding of this RNA structure greatly increased or decreased levels of the partitioning proteins.

The diet rich in fruits is widely recommended for health-promoting properties. Regular consumption of fruits could reduce the risk of many diseases. The fruit-based alcoholic beverages have been produced for centuries and, in many countries, are still very popular. ‘Nalewka’ is a traditional name of Polish liqueur, i.e., an alcoholic beverage made by maceration of fruits. Homemade nalewkas are often stored for a long time before consumption, which can affect the content of valuable secondary metabolites. The aim of the study was to compare the effect of long-time storage on antioxidant activity and total polyphenol, total anthocyanin, tannin as well as gallic acid content in three homemade nalewkas. Cherry, plum, and multifruit nalewka were produced in 1997 and between 2013–2019 years. The antioxidant activity was measured by the DPPH, ABTS, FRAP, and CUPRAC methods. The content of gallic acid was estimated by high-performance liquid chromatography (HPLC). The oldest nalewkas (from 1997) showed significantly lower antioxidant activity as well as a lower content of polyphenols, anthocyanins, and gallic acid compared to the later-produced nalewkas, in particular those produced in 2016–2019. In most cases, a correlation was also found between the parameters of antioxidant activity as well as the total content of polyphenols and anthocyanins. Long-term storage of nalewkas reduces the content of valuable secondary metabolites responsible for the antioxidant activity and, thus, the health properties of the beverage.

Ferulic acid (FA) has been widely used in the pharmaceutical and cosmetics industry due to its, inter alia, antioxidant, antiaging and anti-inflammatory effects This compound added to cosmetic preparations can protect skin because of its photoprotective activity. However, the usefulness of FA as a therapeutic agent is limited due to its low solubility and bioavailability. The paper presents the synthesis, identification, and physicochemical properties of new FA derivatives with propyl esters of three amino acids, glycine (GPr[FA]), L-leucine (LPr[FA]), and L-proline (PPr[FA]). The NMR and FTIR spectroscopy, DSC, and TG analysis were used as analytical methods. Moreover, water solubility of the new conjugates was compared with the parent acid. Both ferulic acid and its conjugates were introduced into hydrogel and emulsion, and the resulting formulations were evaluated for stability. Additionally, in vitro penetration of all studied compounds from both formulations and for comparative purposes using Franz diffusion cells was evaluated from the solution in 70% (v/v) ethanol. Finally, cytotoxicity against murine fibroblasts L929 was tested. All of the analyzed compounds permeated pig skin and accumulated in it. LPr[FA] and PPr[FA] were characterized by much better permeability compared to the parent ferulic acid. Additionally, it was shown that all the analyzed derivatives are characterized by high antioxidant activity and lack of cytotoxicity. Therefore, they can be considered as an interesting alternative to be applied in dermatologic and cosmetic preparations.

Alzheimer’s disease, a neurodegenerative disease, is one of the most common causes of dementia if elderly people worldwide. Alzheimer’s disease leads to the alienation of individuals and their exclusion from social and professional life. It is characterized mainly by the degradation of memory and disorientation, which occurs as a result of the loss of neuronal structure and function in different brain areas. In recent years, more and more attention has been paid to use in the treatment of natural bioactive compounds that will be effective in neurodegenerative diseases, including Alzheimer’s disease. G. biloba L. and its most frequently used standardized extract (EGb 761), have been used for many years in supportive therapy and in the prevention of cognitive disorders. The paper presents an overview of reports on the pathogenesis of Alzheimer’s disease, as well as a summary of the properties of G. biloba extract and its effects on the possible pathogenesis of the disease. By exploring more about the pathogenesis of the disease and the benefits of G. biloba extract for patients with Alzheimer’s disease, it will be possible to create an individualized therapeutic protocol to optimize the treatment.