Search

Neisseria gonorrhoeae causes the sexually transmitted disease gonorrhoea by evading innate immunity. Colonizing the mucosa of the reproductive tract depends on the bacterial outer membrane porin, PorB, which is essential for ion and nutrient uptake. PorB is also targeted to host mitochondria and regulates apoptosis pathways to promote infections. How PorB traffics from the outer membrane of N. gonorrhoeae to mitochondria and whether it modulates innate immune cells, such as macrophages, remains unclear. Here, we show that N. gonorrhoeae secretes PorB via outer membrane vesicles (OMVs). Purified OMVs contained primarily outer membrane proteins including oligomeric PorB. The porin was targeted to mitochondria of macrophages after exposure to purified OMVs and wild type N. gonorrhoeae. This was associated with loss of mitochondrial membrane potential, release of cytochrome c, activation of apoptotic caspases and cell death in a time-dependent manner. Consistent with this, OMV-induced macrophage death was prevented with the pan-caspase inhibitor, Q-VD-PH. This shows that N. gonorrhoeae utilizes OMVs to target PorB to mitochondria and to induce apoptosis in macrophages, thus affecting innate immunity., Author summary Neisseria gonorrhoeae causes the sexually transmitted disease gonorrhoea in more than 100 million people worldwide every year. The bacteria replicate in the reproductive tract by evading innate and adaptive immunity. In the absence of effective vaccines and the rise of antibiotic resistance, understanding the molecular interactions between innate immune cells and N. gonorrhoeae may lead to new strategies to combat bacterial growth and the symptoms of gonorrhoea. It has long been known that the N. gonorrhoeae porin, PorB, promotes bacterial survival but also targets host mitochondria in infections. The mechanism by which PorB traffics form the bacterial outer membrane to host mitochondria remains unclear. Here, we utilized proteomics and super-resolution microscopy to show that N. gonorrhoeae secretes PorB via outer membrane vesicles. These vesicles are taken up by macrophages and deliver PorB to mitochondria. Macrophages treated with N. gonorrhoeae vesicles contained damaged mitochondria and active caspase-3. A caspase inhibitor prevented apoptosis of macrophages treated with N. gonorrhoeae vesicles. This suggests that N. gonorrhoeae secretes membrane vesicles, which are readily detectable in gonorrhoea patients, to target macrophages and to promote infections.