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The interest in adopting more sustainable diets can be a driver for consumers to engage the consumption of edible insects, even in countries where they are not culturally accepted as food. This work aimed to study the perceptions and knowledge of consumers in different countries towards edible insects and their relation with environment and sustainability. The present investigation was based on a questionnaire survey (11 questions) and this descriptive cross-sectional study was carried out on a non-probabilistic sample of 7221 participants from 14 countries. The participants in the survey revealed high knowledge (over 50%) about the sustainability issues related with edible insects as food. The sociodemographic variables education, sex and age are influential, by decreasing order of importance. Regarding living environment, rural areas have the lowest percentage of informed participants. Comparing countries, statistically significant differences were observed for all questions, making this the most important predictor for information about sustainability of edible insects. This is expected, having in mind that cultural influences are highly variable among the set of countries included in the study, with a high number of European countries, but also with Latin American countries like Mexico or Brazil, or Middle East countries like Turkey or Lebanon. info:eu-repo/semantics/publishedVersion

Food behaviour is governed by different kinds of motivations, some of individual nature and others related with the external food environment. This study investigated the eating motivations in sixteen countries with respect to commercial and marketing influences on food choices. The questionnaire survey was developed between September 2017 and June 2018, via online tools, targeting a convenience sample of residents in sixteen countries (Argentina, Brazil, Croatia, Egypt, Greece, Hungary, Italy, Latvia, Lithuania, Netherlands, Poland, Portugal, Romania, Serbia, Slovenia and the United States of America). The number of valid responses received was 11, 919 participants. The data were treated using SPSS software, and the main statistical techniques used included exploratory factor analysis, evaluation of internal reliability through Cronbach’s alpha, cluster analysis (hierarchical and k-means) and logistic regression. The results obtained showed two groups of people: low motivated and notably motivated consumers. The results showed high asymmetries between countries, with highest percentage of highly motivated consumers in Egypt and the lowest percentage of highly motivated in Portugal. It was further observed that consumers more influenced by commercial and marketing aspects (the notably motivated) tend to be women, young, single, less educated, less likely to be professionally active, and those who live mostly in rural or suburban areas. Less exercise and overweight are also factors associated with greater propensity for commercial and marketing motivations. Furthermore, health problems such as shellfish or gluten intolerance, hypertension and high cholesterol confer less propensity to be in the segment of the notably motivated consumers. In conclusion, this work highlighted the role of geographic, sociodemographic and lifestyle factors as food choice determinants.

Resumo: Introdução – O consumo de insetos é uma prática tradicional ao longo da história humana, mas o seu consumo é muito variável de acordo com a região do globo. Objetivos – Pretendeu-se investigar o nível de conhecimento sobre insetos comestíveis numa amostra de participantes de treze países. Métodos – Os dados foram recolhidos em 2021 por questionário online. Obtiveram-se 6899 respostas válidas. Para a análise dos dados usou-se análise fatorial, análise de clusters e testes qui-quadrado. Resultados – Foram usados 27 itens para medir o conhecimento, utilizando uma escala do tipo Likert de cinco pontos. Aplicando análise fatorial obteve-se uma solução que explica 55% da variância total observada. Esta inclui 4 fatores que retiveram 22 dos 27 itens iniciais: F1 = Sustentabilidade (8 it); F2 = Nutrição (8 it); F3 = Fatores de Produção (2 it); F4 = Preocupações com a Saúde (4 it). A análise de clusters produziu três grupos de participantes (indivíduos 'receosos', 'agricultores' e 'ecológicos'). A caracterização dos clusters revelou que a idade não influenciou a inclusão nos clusters, enquanto sexo, escolaridade, país, meio onde reside, área profissional e rendimento influenciaram a composição dos clusters. Conclusões – O nível de conhecimento sobre insetos comestíveis é altamente variável de acordo com as características individuais e localização geográfica. Por outro lado, a segmentação permitiu identificar 3 tipos de indivíduos, 'receosos', 'agricultores' e 'ecológicos'. info:eu-repo/semantics/publishedVersion

Food behaviour is governed by different kinds of motivations, some of individual nature and others related with the external food environment. This study investigated the eating motivations in sixteen countries with respect to commercial and marketing influences on food choices. The questionnaire survey was developed between September 2017 and June 2018, via online tools, targeting a convenience sample of residents in sixteen countries (Argentina, Brazil, Croatia, Egypt, Greece, Hungary, Italy, Latvia, Lithuania, Netherlands, Poland, Portugal, Romania, Serbia, Slovenia and the United States of America). The number of valid responses received was 11,919 participants. The data were treated using SPSS software, and the main statistical techniques used included exploratory factor analysis, evaluation of internal reliability through Cronbach’s alpha, cluster analysis (hierarchical and k-means) and logistic regression. The results obtained showed two groups of people: low motivated and notably motivated consumers. The results showed high asymmetries between countries, with highest percentage of highly motivated consumers in Egypt and the lowest percentage of highly motivated in Portugal. It was further observed that consumers more influenced by commercial and marketing aspects (the notably motivated) tend to be women, young, single, less educated, less likely to be professionally active, and those who live mostly in rural or suburban areas. Less exercise and overweight are also factors associated with greater propensity for commercial and marketing motivations. Furthermore, health problems such as shellfish or gluten intolerance, hypertension and high cholesterol confer less propensity to be in the segment of the notably motivated consumers. In conclusion, this work highlighted the role of geographic, sociodemographic and lifestyle factors as food choice determinants. info:eu-repo/semantics/publishedVersion

Individual eating habits are influenced by a number of factors, including both internal variables such as physiology and emotion, as well as environmental factors such as food availability and cultural norms. Given the public health impact of dietary habits (choice, quality, amount, frequency) on health outcomes, it is important to understand what factors influence eating habits on a societal level. The aim of this research was to determine factors that influence eating habits and compare these factors between four different countries – Latvia, Lithuania, Portugal and the USA. An eating motivation questionnaire was used to measure eating habits in 3,348 respondents from different regions and countries. There were ten parts - demographical information, anthropometric data and behavioral and health related elements, sources of information about healthy eating, factors related to food choices according to motivations (health, emotional, economic, availability, social, cultural, environmental, political, marketing and commercials). Data were analyzed using descriptive statistics, and self-reported motivation was compared across countries. Health was the primary motivator of food selection in this sample (71% of respondents), whereas 34% reported that emotional factors impact their dietary habits and 35% reported that economic factors determine their food selection. A large number of respondents (44%) disagreed or strongly disagree or disagreed with the idea that marketing impacts their dietary habits. Portugal had the highest number of participants (86%), reporting that they agreed or strongly agreed with having health-related motivations for food selection, with Latvia (65%) and Lithuania (76%) showing more moderate levels of endorsement of healthy eating motivations, and the USA having the fewest respondents (52%) endorsing health-related motivations. Respondents from Portugal were more likely than respondents from the other countries to deny having emotional, economic and marketing motivations in food selection. From results can conclude that consumers are motivated by healthiness factors when making food choices (71% of respondents), but marketing, economic and emotional factors positively impact only 30% of consumers, other respondents completely disagreed or was indifferent to these types of motivations. Baltic countries (Latvia and Lithuania) were similar to each other, but Portugal and USA were completely different. Portugal strongly agreed with healthy motivations and disagreed with all other motivations, whereas USA and also Baltic countries had more equal division of opinions regarding impact of different motivations. info:eu-repo/semantics/publishedVersion

In recent years, insects have been suggested as an alternative source of animal protein, and a more sustainable way to provide the needs in protein of the growing world population. Edible insects are nutritive foods, rich in macronutrients and particularly protein and fat, but also contain micronutrients important for many body functions, such as vitamins and dietary minerals. The project "EISuFood – Study about food habits and knowledge about edible insects as sustainable foods" is developed in different countries and aims to study the habits, knowledge, and perceptions of consumers in different social and cultural contexts to edible insects. The project was approved by the CERNAS-IPV Research Centre at the Polytechnic Institute of Viseu, in December 2020, and the Principal investigator and leader of the international team is Raquel Guiné. The project involves a total of 69 researchers in the 18 participating countries (Brazil, Cape Verde, Colombia, Croatia, Greece, Latvia, Lebanon, Lithuania, Mexico, Morocco, Nigeria, Poland, Romania, Serbia, Slovenia, Spain, Turkey, with Portugal being the leader country). The project involves four tasks as follows: 1. Preparation of the instruments for data collection ; 2. Collection of data in the 18 countries ; 3. Analysis of the data ; and 4. Preparation of scientific outputs and dissemination of results. The questionnaire used in the project addresses seven different dimensions: Culture and Tradition, Gastronomic Innovation and Gourmet Kitchen, Environment and Sustainability, Economic and Social Aspects, Commercialization and Marketing, Nutritional Aspects and, finally, Health Effects. The questionnaire was prepared by the whole international team and approved by the Ethics Committee of the Polytechnic of Viseu with Reference No. 45/SUB/2021. An initial screening involved the validation for the Portuguese sample1, before global application in all countries. The validation of this questionnaire confirms its usefulness for investigating consumer perceptions of and knowledge about edible insects, making possible its application in different countries

The adequate intake of dietary fibres is essential to human health. Hence, this work intended to evaluate the level knowledge of about food fibres and investigate what factors might be associated with it. It was undertaken a descriptive cross-sectional study on a sample composed of 6,010 participants from ten different countries. The survey was based on a questionnaire of self-response, approved and complying with all ethical issues. The data collected were subjected to factor analysis and cluster analysis techniques. Validation was made by splitting the data set into two equal parts for confirmation of the results. Factor analysis allowed concluding that ten from the 12 variables used to measure the knowledge about dietary fibre should be grouped into two dimensions or factors: one linked to health effects of dietary fibre (α =0.854) and the other to its sources (α =0.644). Cluster analysis showed that the participants could be divided into three groups: Cluster 1 – Good knowledge both about sources and health effects of dietary fibre; Cluster 2 – Good knowledge about the sources of dietary fibre but poor knowledge about its health effects; Cluster 3 – Poor knowledge both about sources and health effects of dietary fibre. The data was appropriate for analysis by means of factor and cluster analysis, so that two factors and three clusters were clearly identified. Moreover, the cluster membership was found varying mostly according to country, living environment and level of education but not according to age or gender. info:eu-repo/semantics/publishedVersion

Poor diets have proven very detrimental for the maintenance of good health and eating an excessive amount of fast food and highly processed meals can harm the general health status of the population. Hence, this work investigates the habits related to eating outside the home and frequency of eating fast food meals in participants from six different countries. A cross-sectional descriptive study was undertaken on a sample of 4,904 adult participants. For the data treatment, basic statistics were complemented with tree classification analysis, using the CRT algorithm. This study concluded that in the sample under study the frequency of eating out of home was in general low, corresponding to four or fewer times per week for 71.9% of the participants, and the consumption of fast food meals was also acceptable, i.e., with a low frequency of up to two times per week in 88.6% of the respondents. The biggest differences were encountered when comparing participants from different age groups, countries or sexes, and not so much for living environment or education level. Tree classification analysis highlighted the relative importance of the considered factors for eating out and fast food meals, being country and age confirmed as the most influential factors. In the evaluated sample the incidence of eating out and fast food meals were low, which is a good indicator to contribute globally for the good health status of the participants involved in the study. info:eu-repo/semantics/publishedVersion

This is an original research work that is a part of the EATMOT project, which aims to study the eating motivations of different nature in a set of countries all over the world. In this work will be presented some results of the project, regarding three major areas: perceptions about healthy eating, sources of information about healthy diet and healthy motivations for food choice. This is a longitudinal observational study carried out on a non- probabilistic sample with 4870 participants residing in 6 European countries: Hungary, Latvia, Lithuania, Portugal, Romania and Slovenia. Analysis of the data was performed using descriptive statistics and t-test for independent samples or ANOVA with Post-Hoc Tukey HSD, depending on the case. The results obtained allowed concluding that most of the participants have some knowledge about what a healthy diet entails, but still one third did not manifest an opinion for the questions related to healthy eating. Regarding the sources of information about healthy eating, internet was the source more frequently used by a significant part of the participants, while hospitals and health centres were quite irrelevant. This raises some concerns because the internet mighht not always be a reliable source of information. Finally, it was found that the health factors were more influential for the food choices in older people, females, widowed, retired and people working or studying in the area of nutrition. info:eu-repo/semantics/publishedVersion

Introduction: The ingestion of fruits, vegetables and cereals, especially whole grain, is associated with a healthy lifestyle and has been recognized as having multiple health benefits, associated, among others, to the ingestion of adequate amounts of dietary fibre. Objective: The aim of this work was to evaluate some eating habits related to fibre rich foods in six different countries: Argentina, Croatia, Hungary, Latvia, Portugal and Romania. Methods: A cross-sectional descriptive study was carried out by means of questionnaire survey applied to a sample of 4905 participants, all over the age of 18 years old. The preparation and application of the questionnaire followed the necessary ethical guidelines and the treatment was made by SPSS. Results: The results showed that for the global sample was observed a low ingestion of salads and vegetables (78.2%), being this particularly problematic for Croatia (86.6%). Regarding the consumption of fruits, for the whole sample the great majority also showed a low consumption (92.3%), and for Latvian participants the percentage is very much expressive (98.3%). As for the consumption of whole cereals, most participants also showed a low consumption, either for the global sample (72.6%) or in the different countries, and particularly for Latvia (90.0%). Some eating habits were also studied and it was observed that for the whole sample 71.9% showed a low frequency of meals ate out of home, while 88.6% revealed acceptable frequency of eating fast food, i.e, only once or twice a week. Conclusions: The results indicated that in the countries at study the ingestion of foods rich in dietary fibre is very low, and therefore it is necessary to implement strategies to increase the consumption of such foods. info:eu-repo/semantics/publishedVersion

Introduction: Dietary fibre (DF) is recognized as healthy for long, so that health claims are allowed by the European Food Safety Authority (EFSA) due to its proved benefits, extended but not only confined to many diseases of the gastrointestinal tract. Objective: This work intended to analyse the level of information about DF in 10 countries, situated in Europe, Africa and South America. Methods: A descriptive cross-sectional study was undertaken through a survey based on a questionnaire of self-response applied to a sample of 6010 participants. The data were lately treated by factor and cluster analyses, including validation methodologies. Results: Factor analysis showed that ten of the twelve items used to assess the knowledge about DF could be arranged into two factors: one related to health effects (α =0.854) and the other to the sources (α =0.644). Furthermore, cluster analysis showed that the participants could be divided into three groups: 1) Good knowledge about sources and health effects of DF; 2) Good knowledge about the sources of DF but poor knowledge about the health effects; 3) Poor knowledge about the sources and health effects of DF. Conclusions: The results clearly allowed identifying two factors and three clusters, and the variables that most influenced cluster membership were country, living environment and level of education. info:eu-repo/semantics/publishedVersion

Article The aim of the study was to analyse the quality of extruded cereals enriched with plant by-products and to obtain fermented drinks from production rejects. Extrusion was performed with co-rotating twin-screw extruder (compression ratio 8:1) at MILZU Ltd. from rye and oat flour (80:20, control samples) with addition of apple (ABF), carrot (CBF) and pumpkin (PBF) by-product flour in various amounts (10%, 15% and 20%). Naturally fermented kvass production process was used for non-alcoholic fermented beverage production. Total dietary fibre (TDF), textural properties and sensory features of extruded products after addition of by-products (BP) were determined. Dry matter, active acidity and sensory properties were analysed in fermented beverages. The obtained results showed a 12-55% increase in TDF of extruded cereals (11.8 g 100 g -1 ) after addition of plant by-products. All extruded samples with BP showed lower hardness levels than control (35.55 ± 2.95 N); samples with PBF were the least hard (P < 0.05). Samples with the lowest bulk density were obtained by the addition of 10% and 15% PBF, and 15% CBF, whereas addition of apple by-product flour in all tested concentrations gave the samples a higher bulk density compared to control. Highest taste and aftertaste scores using 5-point hedonic scale were given to samples with addition of 15% and 20% ABF, which also showed high consumer acceptance. With regards to fermented drinks, the highest dry matter content was found in PBF and ABF drink, 8.1 ± 0.1 and 7.0 ± 0.1, respectively. Sensory evaluation of fermented beverages showed that the intensity of flavour, acidity and aroma was most pronounced in sample with ABF, whereas colour was most pronounced in sample with PBF. In order to reduce production costs, it is possible to use production rejects of extruded cereals enriched with plant by-products to obtain new products.

Article High pressure processing (HPP) is widely used as an alternative to thermal food preservation technologies, including processed meats treatment. This technology affects food texture and water-holding capacity, which may have beneficial effect on product yield. After thermal treatment, meat partially releases water together with water-soluble proteins, which is concerned as a loss. It is very important not only because of changes in taste properties, but also economic aspects such as reduced final product weight. The aim of the study was to evaluate changes in the meat microstructure and water-holding capacity upon high pressure treatment. Pork samples were treated at various pressures and holding times, namely, 300 and 600 MPa with a 1 and 15 minutes holding time at each pressure. Untreated sample was regarded as a control. Microstructure of pork meat was evaluated after the paraffination of the samples. Fibre cross section area and space between fibres were measured and reported. Water-holding capacity was measured by centrifugation of meat samples over filter and calculating released amount of juice. Results indicated that fibre size did not change significantly after treatment at 300–600 MPa pressure comparing to the control sample – untreated meat. However, high pressure can affect hydrophobic properties of myofibrillar protein. The experimental results showed that waterholding capacity increases with the high pressure treatment. It is an important issue in meat processing industry, because HPP treatment allows reducing the water loss in fresh pork.

Article Consumer interest in breakfast cereal flakes has increased during the last few years. Various technologies, used to produce flakes, significantly influence their quality parameters and shelf - life stab ility. The main purpose of the present research was to investigate how different processing methods affect the physical parameters of triticale flakes. For obtaining the flakes, cleaned whole triticale grains were treated using the following technologies: dry processing (hot air), steam processing and soaking with subsequent steaming. For preparing the flakes different kilning methods and traditional flaking rolls were used. Traditionally made rolled and dried whole grain triticale flakes were analysed as a control sample. Using standard methods, the flakes’ moisture content, water activity, microstructure, swelling capacity and colour changes were analysed. The gap settings of flake rollers do not influence significantly ( P < 0.05) changes of starch during processing. However, the starch granules were fully transformed into sugars in the flake samples with greater thickness. Non - significant ( P < 0.05) steaming and hot air drying (toasting) conditions’ effects were observed on the changes of the starch granules during processing. Strong correlation was determined during the analysis of water activity and moisture content. The moisture content of the ready - to - eat flakes varied from 2.54% to 10.66%, and the water activity value was from 0.108 to 0.494. Compared with traditionally processed flakes (control sample) the colour of the flakes prepared using other technologies changed significantly, the  E values varied from 9.587 to 18.554. The colour of the soaked - steamed - rolled - hot air dried samples was similar but those significantly differed from the colour of soaked - dried - rolled - hot air dried flake samples. The soaked - dried - rolled - hot air dried flakes were darker compared with other analysed flake samples.

It is well known that dietary fibre (DF) is recognized as very important to maintain health and prevent some chronic diseases. Therefore, the objective of the present study was to evaluate the level of knowledge about the effect for the human body derived from ingesting DF. For that purpose, a descriptive cross-sectional study was carried out on a sample of 6010 participants, from 10 countries. The results obtained were compiled into a variable that accounted for the general level of knowledge, and this showed that globally the level of information about the benefits of DF was acceptable (average score of 3.54+/-0.5, on a scale from 1 to 5). Some of the variables considered from the study were gender, level of education, living environment and country, for which significant differences were found between groups. The highest level of knowledge was found for women, with university degree, from Portugal and living in urban areas. As a conclusion, it was possible to see that, in general, the participants in the study were quite well informed about the benefits of DF for the improvement of human health.

Article Th e aim of current research was to assess the nutritional value of breakfast cereals containing germinated spring grain flakes and its changes after 6 month storage. Three types of breakfast cereals were prepared and packaged in two types of Standup pouches – Pap50g/Alu7/Pe60 (AL), Pap40g/PELD20/PE40 (PE). For the accelerated shelf life test the samples were stored at 35 ± 2 °C and dietary fibre, protein, fat, B - group vitamins, sugars, total phenol content and DPPH, ABTS+ radical scavenging activity were dete rmined. Developed breakfast cereals have high nutritional value and all are high in fibre and thiamine. Additionally, sample S2 is source of protein, riboflavin, niacin, and S3 – is source of riboflavin and high in niacin. Comparing total phenolic content and antioxidant capacity of tested samples S3 showed the highest values. Storage and selected packaging influenced stability of nutrients, and for S1 and S2 AL showed bett er results whereas for S3 – PE.

Dietary fibre (DF) has been recognized as a major determinant for improvement of health. Hence the means of information through which people become aware of its benefits are crucial. This work aimed at studying the sources of information about DF, as a means to educate people about aspects related to healthy eating. Factors such as gender, level of education, living environment or country were evaluated as to their effect on the selection of sources and preferences. For this, a descriptive cross-sectional study by means of a questionnaire, applied to a non-probabilistic sample of 6010 participants from 10 countries in different continents (Europe, Africa and America), answered after informed consent by all participants. For the analysis were used several descriptive statistics tools, crosstabs and chi square test to assess the relations between some of the variables under study. The results showed that mostly the information about DF comes from the internet, but the participants recognize that television might be a most suitable way to disseminate information about DF. The results also indicated differences between genders, levels of education, living environments and countries. The internet, the preferred source of information, got highest scores for Hungary, for urban areas, for university level of education and for female gender. The radio, the least scored source of information, was preferred in Egypt, for men and with lower education (primary school). As a conclusion, people get information through the internet due to easy access. However, it is to some extent a risk given the impossibility to control de information made public on the internet. The role of health centers and hospitals as well as schools should definitely be increased, as a responsible way to ensure correct information.

The positive benefits of DF associated with the regulation of the intestinal transit, and the prevention or treatment of diabetes, cardiovascular diseases and regulate colon cancer and obesity. The aim of this research was to collect information on knowledge about dietary fibre on citizens’ of Latvia. The questionnaire consists 22 questions, which aim was to evaluate knowledge on three distinct areas: six for knowledge about food fibres; six for the relation between fibres and variety of foods and 12 for the relations between fibres and diseases. The methodological study was conducted with 231 participants, from which 64.9% were female and 35.1% male, aged between 17 and 80 years. The respondents were selected by convenience, although of age, literacy, gender, geographical area of residence, including people from different cities and smaller villages. 67% of respondents consider that the consumption of sufficient amounts of fibres can prevent or treat different diseases. 85% of respondents have noted that legumes (peas, beans) are source of fibres, but more possible take up the fibre with a combination of vegetables and animal products. This research was prepared in the ambit of the multinational project from CI&DETS Research Centre (IPV e Viseu, Portugal) with reference PROJ/CI&DETS/2014/0001.